Standard name
Human Ortholog
Description SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.09 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.09 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.17 0.16 0.1 0 0 0 0 0 0
Cytoplasm 0.87 0.86 0.9 0.91 0.81 0.77 0.7 0.71 – 0.7 0.64 0.62 0.96 0.99 0.99 0.85 0.92 0.9 0.82 0.74 0.81 0.72 0.82 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.14 0.29 0.42 0.45 – 0.41 0.61 0.56 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.07 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 14 16 3 3 10 1 0 – 0 0 0 2 0 0 4 4 2 4 2 5 1 2 2
Bud 0 4 3 3 6 13 23 18 – 5 14 19 1 0 0 1 0 1 6 6 9 3 5 6
Bud Neck 1 0 3 1 1 3 8 4 – 7 1 3 2 0 0 1 0 0 7 14 16 17 7 5
Bud Site 7 1 9 9 11 12 10 2 – 4 0 3 0 1 0 0 2 0 – – – – – –
Cell Periphery 2 4 3 8 1 2 1 4 – 7 2 2 5 15 6 9 16 7 0 0 0 0 0 2
Cytoplasm 153 134 251 267 312 271 407 357 – 316 195 299 219 431 440 46 89 64 151 124 234 135 267 369
Endoplasmic Reticulum 1 5 1 1 4 6 0 0 – 1 0 3 4 2 2 3 5 6 0 1 1 7 8 7
Endosome 1 0 0 1 16 1 5 2 – 4 0 1 1 0 0 0 0 0 4 3 3 4 0 3
Golgi 0 1 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0 0 0 0 0 0 1 2
Mitochondria 1 0 6 13 54 103 241 226 – 183 186 270 0 0 0 2 3 0 0 4 7 4 13 7
Nucleus 0 1 0 0 1 2 3 4 – 3 1 1 0 0 1 0 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 1 0 3 – 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 5 6 8 12 15 6 4 1 – 0 1 0 0 3 0 1 0 1 0 0 1 0 0 0
SpindlePole 4 1 2 13 28 13 27 8 – 14 5 14 2 4 0 1 0 2 4 2 3 2 0 1
Vac/Vac Membrane 1 1 0 1 7 3 5 5 – 5 1 2 0 1 1 0 1 2 2 4 1 8 11 13
Unique Cell Count 175 155 279 294 387 352 579 502 449 306 485 228 437 445 54 97 71 185 169 290 188 325 430
Labelled Cell Count 188 172 302 332 460 446 735 635 549 407 620 236 457 452 68 122 85 185 169 290 188 325 430


Bud Neck, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 6.4 4.6 4.7 4.4 3.9 3.4 3.3 – 3.2 3.0 3.0 6.9 6.7 6.7 8.3 8.1 7.9 7.4 7.4 7.4
Std Deviation (1e-4) 0.7 1.2 0.6 1.2 1.3 1.1 1.2 1.0 – 1.3 1.2 0.7 1.3 0.9 1.4 1.7 1.4 1.3 1.6 1.2 1.3
Intensity Change (Log2) – – – 0.05 -0.06 -0.24 -0.41 -0.45 – -0.52 -0.58 -0.62 0.6 0.54 0.56 0.87 0.83 0.8 0.69 0.7 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 -2.9 -5.1 -5.1 0 -0.6 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 2.0 0 0 0 0
Cytoplasm 0.3 -3.3 -4.3 -6.4 -6.1 – -6.2 -7.4 -8.4 2.6 5.3 5.6 -1.0 0.5 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8136 1.7026 1.8239 1.3987 1.6368 1.6494 3.155 3.5928 3.2868 2.7347 2.7618 3.2607 -0.1072 0.6984 0.1781 0.6092 -0.2265 0.3741
Actin 0.0397 0.0011 0.0462 0.0268 0.0315 0.0112 0.0504 0.001 0.0224 0.0003 0.0078 0.013 0.0516 0.0043 0.0255 0.0086 0.0026 0.006
Bud 0.0056 0.0152 0.0021 0.0206 0.0024 0.003 0.0006 0.0069 0.0016 0.0004 0.0005 0.0002 0.0006 0.0012 0.0016 0.0106 0.0011 0.0002
Bud Neck 0.0172 0.0096 0.0042 0.001 0.0027 0.3327 0.0311 0.0023 0.0027 0.0008 0.0245 0.3304 0.0187 0.0009 0.0016 0.0099 0.0003 0.5152
Bud Periphery 0.0042 0.0038 0.0041 0.012 0.0138 0.0026 0.0007 0.0118 0.0019 0.0003 0.0004 0.0004 0.0007 0.0016 0.0022 0.0451 0.0011 0.0004
Bud Site 0.0158 0.0496 0.0055 0.0079 0.0043 0.0009 0.0501 0.0314 0.0175 0.0035 0.0023 0.0011 0.0221 0.0213 0.0305 0.0576 0.0038 0.0005
Cell Periphery 0.0014 0.0008 0.0011 0.0004 0.0005 0.0006 0.0013 0.0008 0.0006 0.0001 0.0002 0.0005 0.0009 0.0007 0.0008 0.0037 0.0001 0.0002
Cytoplasm 0.5394 0.7184 0.5915 0.4556 0.5003 0.4994 0.5378 0.7224 0.6437 0.6284 0.6936 0.4838 0.5729 0.7276 0.6236 0.6929 0.5836 0.389
Cytoplasmic Foci 0.0375 0.0219 0.0239 0.0462 0.059 0.0141 0.0176 0.013 0.0185 0.0487 0.0719 0.0208 0.0506 0.0071 0.0123 0.0154 0.0341 0.0159
Eisosomes 0.0005 0.0001 0.0004 0.0002 0.0002 0.0002 0.0005 0 0.0001 0 0 0.0003 0.0004 0.0001 0.0004 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0091 0.0026 0.0055 0.0042 0.0056 0.0025 0.0061 0.0024 0.0038 0.0028 0.0024 0.0019 0.0051 0.0042 0.0043 0.0039 0.0042 0.0046
Endosome 0.0356 0.0045 0.0227 0.0469 0.051 0.0046 0.013 0.005 0.0086 0.0033 0.0123 0.0042 0.0091 0.002 0.007 0.0224 0.031 0.0108
Golgi 0.0166 0.0011 0.0148 0.0087 0.0213 0.0021 0.015 0.0006 0.0032 0.0003 0.0031 0.0013 0.0059 0.0003 0.0019 0.0037 0.0042 0.0023
Lipid Particles 0.0113 0.0008 0.0059 0.0125 0.0069 0.0026 0.0044 0.0002 0.0065 0.0003 0.0018 0.0007 0.0073 0.0002 0.0044 0.0061 0.001 0.0091
Mitochondria 0.0096 0.0004 0.0055 0.0153 0.0059 0.0197 0.0024 0.0003 0.0025 0.0001 0.0006 0.0003 0.0015 0.0001 0.0019 0.0094 0.0029 0.0009
None 0.2182 0.1624 0.2525 0.2735 0.1976 0.0927 0.2566 0.1952 0.2486 0.299 0.1702 0.1337 0.2302 0.2248 0.2707 0.0798 0.271 0.0255
Nuclear Periphery 0.0054 0.0004 0.0011 0.0032 0.0224 0.0045 0.0017 0.0004 0.0012 0.0006 0.001 0.004 0.0031 0.0004 0.001 0.0011 0.021 0.0049
Nucleolus 0.0007 0.0003 0.0003 0.0008 0.0021 0.0003 0.0003 0.0002 0.0005 0.0001 0.0001 0.0001 0.0005 0.0001 0.0013 0.0053 0.0006 0.0006
Nucleus 0.0053 0.0015 0.0013 0.0036 0.0046 0.0009 0.0009 0.0013 0.0009 0.0009 0.0007 0.0009 0.0024 0.0005 0.0014 0.0024 0.0108 0.0016
Peroxisomes 0.0172 0.0018 0.0062 0.0412 0.015 0.0023 0.0042 0.0002 0.0108 0.0016 0.0029 0.0003 0.0099 0.0001 0.0019 0.0049 0.0031 0.0033
Punctate Nuclear 0.0038 0.0016 0.0014 0.0114 0.0413 0.0009 0.002 0.0006 0.001 0.0058 0.002 0.0009 0.004 0.0003 0.0021 0.0007 0.0182 0.0078
Vacuole 0.0044 0.002 0.003 0.0065 0.0095 0.001 0.0029 0.0036 0.0032 0.0023 0.0016 0.0007 0.002 0.0021 0.0032 0.0141 0.0043 0.0008
Vacuole Periphery 0.0015 0.0002 0.0007 0.0014 0.0024 0.0014 0.0004 0.0002 0.0004 0.0003 0.0003 0.0003 0.0003 0.0001 0.0004 0.002 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.5857 11.504 34.1459 70.1526 21.7983 10.3724 11.2565 15.0681 47.7874 31.472
Translational Efficiency 0.6057 0.5755 0.5506 0.5963 0.5676 0.7108 0.4775 0.4598 0.6223 0.5495

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1667 353 2132 4 830 6 2704 2265 2497 359 4836 2269

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 760.51 788.69 919.57 347.24 760.60 904.36 952.28 940.02 760.54 790.62 937.86 938.97
Standard Deviation 95.16 127.98 131.86 7.03 94.76 135.52 119.80 110.29 95.03 128.97 126.31 112.97
Intensity Change Log 2 — 0.052491 0.273992 -1.131034 — 0.249759 0.324248 0.305554 — 0.154500 0.299340 -0.240821

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000260 0.000816 0.000236 0.000124 0.000635 0.000400 0.000376 0.000187 0.000385 0.000809 0.000314 0.000187
Bud Neck 0.053056 0.032955 0.078436 0.000003 0.061324 0.039452 0.086630 0.055155 0.055804 0.033064 0.083018 0.055058
Bud Site 0.033673 0.066915 0.106284* 0.000009 0.053374 0.226940* 0.108769* 0.050964 0.040222 0.069590 0.107674* 0.050874
Cell Periphery 0.000152 0.000177 0.000078 0.000093 0.000245 0.000271 0.000078 0.000056 0.000183 0.000179 0.000078 0.000056
Cytoplasm 0.585687* 0.543154* 0.621665* 0.000011 0.450582* 0.403424* 0.630856* 0.691385* 0.540778* 0.540819* 0.626804* 0.690167*
Cytoplasmic Foci 0.187472* 0.081201 0.025996 0.000005 0.239467* 0.155386 0.022084 0.026589 0.204755* 0.082441 0.023808 0.026542
Eisosomes 0.000184 0.000075 0.000021 0.000315 0.000282 0.000117 0.000024 0.000026 0.000216 0.000076 0.000023 0.000026
Endoplasmic Reticulum 0.000251 0.000438 0.000383 0.000000 0.001109 0.001081 0.000201 0.000193 0.000536 0.000448 0.000281 0.000193
Endosome 0.007056 0.032603 0.004380 0.000000 0.033387 0.003880 0.003866 0.003334 0.015808 0.032123 0.004093 0.003328
Golgi 0.003284 0.006525 0.000340 0.000002 0.007616 0.000714 0.000813 0.000372 0.004724 0.006428 0.000605 0.000372
Lipid Particles 0.001244 0.002659 0.000383 0.000010 0.003821 0.000732 0.000242 0.000245 0.002100 0.002627 0.000305 0.000245
Mitochondria 0.002126 0.003217 0.000253 0.000005 0.003269 0.000034 0.002041 0.000209 0.002506 0.003164 0.001252 0.000209
Mitotic Spindle 0.000576 0.004758 0.015880 0.000135 0.001058 0.000084 0.017773 0.001954 0.000736 0.004680 0.016939 0.001950
None 0.004221 0.001445 0.002333 0.999159* 0.000743 0.000049 0.001308 0.000966 0.003065 0.001421 0.001760 0.002726
Nuclear Periphery 0.000054 0.000049 0.000286 0.000000 0.000161 0.000037 0.000154 0.000135 0.000089 0.000049 0.000212 0.000134
Nuclear Periphery Foci 0.000509 0.000376 0.000503 0.000001 0.000532 0.000489 0.000248 0.000414 0.000517 0.000378 0.000360 0.000413
Nucleolus 0.000367 0.000169 0.000337 0.000001 0.000731 0.000066 0.000174 0.000126 0.000488 0.000167 0.000246 0.000126
Nucleus 0.051203 0.007171 0.039659 0.000003 0.019823 0.041934 0.022769 0.046759 0.040773 0.007752 0.030215 0.046676
Peroxisomes 0.003336 0.008717 0.001756 0.000124 0.009059 0.001622 0.001775 0.001774 0.005238 0.008598 0.001767 0.001771
Vacuole 0.064664 0.205185* 0.100215 0.000000 0.110959 0.123136 0.098306 0.118867 0.080053 0.203813* 0.099148 0.118658
Vacuole Periphery 0.000623 0.001394 0.000575 0.000000 0.001824 0.000152 0.001512 0.000291 0.001022 0.001373 0.001099 0.000290

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -1.74 2.00 3.86 2.69 2.07
Bud Neck NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 4.95 1.59 3.67 -1.80 2.70
Bud Site NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -3.55 -8.09 0.85 3.85 8.03
Cell Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.06 7.44 8.36 2.45 5.02
Cytoplasm NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 0.21 -8.14 -15.75 -8.20 -9.34
Cytoplasmic Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 18.49 45.94 44.11 9.85 -3.80
Eisosomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 5.47 7.70 7.61 5.44 -1.71
Endoplasmic Reticulum NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 0.46 1.32 3.23 0.77 5.95
Endosome NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -3.63 14.23 14.03 7.22 0.76
Golgi NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.58 11.60 11.93 2.88 1.43
Lipid Particles NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.16 6.30 7.05 1.20 8.49
Mitochondria NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.24 3.51 5.61 1.74 3.32
Mitotic Spindle NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -1.57 -11.91 -1.70 1.11 10.65
None NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 1.61 3.22 0.27 -1.07 -1.04
Nuclear Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 1.41 -15.61 -5.69 -8.82 14.41
Nuclear Periphery Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 1.05 0.70 1.28 -0.56 1.00
Nucleolus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 2.03 1.41 2.24 1.02 5.58
Nucleus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN 13.20 -7.77 -7.80 -18.03 -1.26
Peroxisomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -1.33 10.29 7.92 3.18 -0.33
Vacuole NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -12.04 -25.15 -16.43 5.08 4.64
Vacuole Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN -0.83 1.82 6.80 3.49 2.33