Standard name
Human Ortholog
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.14 0.07 0.07 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0.12 0.14 0.12 0.33 0.34 0.36 0.48 0.55 0.4 0 0 0 0.08 0.1 0.05 0 0 0 0.07 0.15 0.17
Nucleus 0 0.1 0 0 0 0 0 0 0.05 0 0 0.08 0 0.06 0.15 0.15 0.13 0 0 0 0 0 0
Nuclear Periphery 0.83 0.81 0.84 0.77 0.76 0.65 0.64 0.61 0.6 0.49 0.57 0.88 0.85 0.67 0.61 0.57 0.54 0.87 0.82 0.87 0.82 0.71 0.61
Nucleolus 0.11 0.07 0.19 0.26 0.19 0.31 0.3 0.31 0.35 0.39 0.35 0.09 0.08 0.06 0.11 0.14 0.14 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.28 0.23 0.17 0.08 0.16 0.06 0.06 0.05 0 0 0.06 0.16 0.17 0.29 0.28 0.25 0.26 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 4 0 1 2
Bud 0 0 0 0 0 0 1 4 1 8 1 0 0 0 0 1 0 1 1 3 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 1 6 8 5 0 0 2 0 1 0
Cell Periphery 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 3 8 9 10 14 10 10 8 8 1 1 8 30 20 17 18 0 0 1 0 0 0
Endoplasmic Reticulum 6 0 2 2 1 1 0 4 3 0 2 2 1 0 10 8 6 0 0 1 0 1 2
Endosome 3 0 13 4 10 14 21 21 10 6 16 1 1 5 5 10 3 1 5 8 1 12 14
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 1 7 11
Mitochondria 11 37 51 39 31 152 177 236 334 300 230 0 1 0 23 24 7 8 9 9 8 47 45
Nucleus 11 26 6 12 7 13 9 21 35 15 16 7 5 12 42 36 19 6 2 3 1 0 0
Nuclear Periphery 204 216 347 214 204 298 334 405 414 264 325 75 112 143 169 135 78 236 250 407 100 229 167
Nucleolus 26 18 78 72 52 142 157 202 243 211 203 8 10 13 31 32 21 2 8 4 2 3 3
Peroxisomes 0 0 0 0 0 1 3 1 2 1 2 0 0 0 0 1 0 0 0 0 0 4 5
SpindlePole 0 0 1 0 1 2 4 2 4 3 2 1 1 8 0 2 0 0 1 3 0 1 0
Vac/Vac Membrane 68 61 69 23 44 29 30 35 16 17 36 14 23 63 78 59 37 11 20 8 2 6 12
Unique Cell Count 246 268 412 279 269 459 520 659 692 544 573 85 132 214 275 236 145 272 305 467 122 323 273
Labelled Cell Count 336 361 575 375 362 667 748 942 1076 841 839 109 164 276 378 326 189 272 305 467 122 323 273


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.9 12.7 12.3 11.5 10.7 9.0 8.5 8.5 7.5 7.4 8.0 12.2 11.1 11.5 8.2 8.8 10.6 13.0 13.9 14.7
Std Deviation (1e-4) 3.8 3.3 3.0 3.4 3.4 2.8 2.1 2.5 2.3 2.3 2.3 2.9 2.8 3.5 2.3 3.1 3.6 2.8 2.7 2.8
Intensity Change (Log2) -0.09 -0.2 -0.44 -0.53 -0.54 -0.72 -0.73 -0.63 -0.01 -0.15 -0.1 -0.59 -0.48 -0.21 0.08 0.17 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 1.4 1.0 0 -0.5 -1.1 -0.6 0 0 0 6.0 3.5 3.3 5.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.4 0.4 -0.1 0.7 0 -1.9 -2.3 -0.3 0 0 -0.6 -1.1 0.7 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 -0.3 7.2 7.6 8.4 12.1 13.6 9.5 -3.4 -4.0 -5.4 -1.7 -0.8 -2.6
Nucleus 2.3 1.1 1.4 0.3 1.8 3.1 1.4 1.4 0 0 2.9 7.0 6.9 5.8
Nuclear Periphery -2.5 -2.7 -6.5 -6.8 -7.9 -8.5 -11.4 -9.1 0.9 0.2 -5.0 -6.8 -7.6 -7.4
Nucleolus 2.2 0.1 4.1 3.9 4.2 5.7 6.6 5.7 -2.1 -3.1 -4.3 -2.7 -1.8 -1.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.2 -0.1 -4.9 -5.4 -6.1 -8.7 -7.3 -5.2 -0.1 0.2 3.7 3.6 2.5 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.6512 9.6575 9.3608 8.8665 9.0529 9.1773 11.2245 12.0531 10.5861 9.7671 9.7166 9.9282 13.2286 12.0901 11.7687 11.0826 11.2475 11.2944
Actin 0.0129 0.0001 0.0044 0.0094 0.0007 0.0012 0.0004 0.0001 0.0168 0.0007 0.0005 0.0001 0.0003 0 0.0002 0.0001 0.0002 0
Bud 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0
Bud Neck 0.0012 0 0.0001 0.0003 0.0002 0 0.0002 0 0.0009 0 0 0 0.0001 0 0 0 0 0
Bud Periphery 0.0002 0 0.0001 0.0006 0.0002 0 0.0001 0 0.0003 0.0002 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0
Bud Site 0.0007 0 0.0001 0.0005 0.0001 0 0.0009 0 0.0007 0 0 0 0.0001 0 0 0 0 0
Cell Periphery 0.0001 0 0 0.0001 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0026 0.0001 0.0004 0.0002 0.0001 0 0.0016 0 0.0012 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasmic Foci 0.0019 0 0.0004 0.0031 0.0001 0.0003 0.0251 0 0.0009 0 0.0007 0 0.0004 0 0.0002 0 0.0001 0.0001
Eisosomes 0.0002 0 0 0.0002 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0024 0.0002 0.0005 0.0011 0.0015 0.0002 0.0005 0.0011 0.0056 0.0017 0.0005 0.0003 0.0005 0.0005 0.0004 0.0005 0.0002 0.0001
Endosome 0.0031 0.0002 0.0004 0.0022 0.0049 0.0012 0.0181 0.0002 0.0027 0.0004 0.0007 0.0002 0.0011 0.0003 0.0002 0.0001 0.0002 0.0001
Golgi 0.0011 0 0.0006 0.0017 0.0012 0.0008 0.0056 0.0001 0.0006 0.0003 0.0009 0.0001 0.0003 0.0001 0.0002 0.0001 0.0006 0.0001
Lipid Particles 0.0021 0.0002 0.0009 0.0069 0.0038 0.0012 0.0027 0.0008 0.0025 0.0014 0.0176 0.0005 0.0034 0.001 0.0091 0.0008 0.0197 0.002
Mitochondria 0.0014 0.0006 0.0007 0.0045 0.002 0.0005 0.0007 0.0011 0.0019 0.0015 0.0028 0.0014 0.0011 0.0132 0.0051 0.0005 0.0005 0.0013
None 0.0051 0 0.0002 0.0006 0.0002 0.0001 0.0002 0.0001 0.0005 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Nuclear Periphery 0.9142 0.9085 0.9412 0.9202 0.8707 0.9703 0.9057 0.926 0.8998 0.9457 0.9074 0.978 0.9515 0.9455 0.9454 0.9647 0.94 0.9793
Nucleolus 0.002 0.0018 0.0012 0.0009 0.0602 0.001 0.0002 0.0003 0.0006 0.0006 0.0041 0.0003 0.0041 0.0001 0.0004 0.0002 0.0069 0.0006
Nucleus 0.018 0.0644 0.021 0.0189 0.0256 0.0089 0.0011 0.0202 0.0167 0.0037 0.0081 0.0026 0.004 0.0015 0.0021 0.002 0.0023 0.0018
Peroxisomes 0.0017 0 0.0005 0.0068 0.0001 0.0004 0.0035 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0 0
Punctate Nuclear 0.0046 0.0004 0.0028 0.0009 0.0013 0.0009 0.0008 0.0003 0.0009 0.0009 0.0301 0.0004 0.0025 0.0001 0.0003 0.0004 0.0017 0.0009
Vacuole 0.0008 0.001 0.0002 0.0005 0.0017 0.0002 0.0004 0.0004 0.0007 0.0005 0.0004 0.0003 0.0005 0.0007 0.0003 0.0002 0.0004 0.0002
Vacuole Periphery 0.0234 0.0223 0.0243 0.0205 0.0252 0.0128 0.0321 0.0492 0.0462 0.0419 0.0254 0.0157 0.0295 0.0367 0.0357 0.0302 0.0267 0.0133

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0547 31.2058 27.5188 31.4529 25.2538 19.7198 33.0384 30.2459 27.7098 34.8855
Translational Efficiency 0.904 0.8521 0.8213 0.7676 0.8443 1.0067 0.7845 0.711 0.6808 0.6133

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
211 1275 248 342 2580 2116 2485 1125 2791 3391 2733 1467

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1419.61 1066.35 1515.47 1329.34 1107.01 1205.17 1436.27 1225.39 1130.64 1152.97 1443.46 1249.62
Standard Deviation 201.90 260.75 208.23 218.63 191.67 203.30 226.98 198.71 209.45 236.38 226.49 208.22
Intensity Change Log 2 -0.412814 0.094271 -0.094784 0.122568 0.375659 0.146573 -0.153551 0.224357 0.015962

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000471 0.001446 0.000647 0.000325 0.000313 0.000361 0.000449 0.000926 0.000325 0.000769 0.000467 0.000786
Bud Neck 0.003329 0.001439 0.002005 0.000950 0.000377 0.000435 0.000572 0.001950 0.000600 0.000812 0.000702 0.001717
Bud Site 0.002535 0.000646 0.000153 0.000060 0.000051 0.000103 0.000032 0.000269 0.000239 0.000307 0.000043 0.000220
Cell Periphery 0.000076 0.000210 0.000030 0.000017 0.000025 0.000029 0.000023 0.000049 0.000029 0.000097 0.000024 0.000042
Cytoplasm 0.000191 0.002707 0.000185 0.000061 0.000062 0.000189 0.000218 0.001034 0.000072 0.001136 0.000215 0.000807
Cytoplasmic Foci 0.002769 0.003280 0.000393 0.000862 0.000389 0.000761 0.000129 0.000895 0.000569 0.001708 0.000153 0.000887
Eisosomes 0.000011 0.000025 0.000010 0.000006 0.000008 0.000006 0.000005 0.000012 0.000008 0.000013 0.000005 0.000011
Endoplasmic Reticulum 0.002486 0.007760 0.002884 0.001893 0.001965 0.002052 0.000727 0.004465 0.002004 0.004198 0.000923 0.003865
Endosome 0.003848 0.003475 0.000428 0.000364 0.000482 0.000697 0.000114 0.001772 0.000736 0.001741 0.000142 0.001444
Golgi 0.005739 0.002609 0.000253 0.001042 0.000189 0.000332 0.000090 0.000695 0.000608 0.001188 0.000105 0.000776
Lipid Particles 0.001409 0.003850 0.000253 0.000556 0.001392 0.002054 0.000153 0.000579 0.001393 0.002729 0.000162 0.000574
Mitochondria 0.004275 0.009327 0.001979 0.001726 0.004888 0.005250 0.002614 0.004351 0.004842 0.006783 0.002557 0.003739
Mitotic Spindle 0.112816* 0.003971 0.023404 0.004178 0.001420 0.001286 0.002646 0.026166 0.009841 0.002296 0.004530 0.021040
None 0.000169 0.006364 0.000209 0.000159 0.000434 0.000367 0.000236 0.000851 0.000414 0.002622 0.000233 0.000690
Nuclear Periphery 0.559064* 0.485689* 0.648042* 0.637597* 0.641515* 0.692055* 0.763257* 0.473885* 0.635282* 0.614462* 0.752802* 0.512051*
Nuclear Periphery Foci 0.225407* 0.288431* 0.189970 0.290732* 0.301628* 0.249015* 0.158854 0.250290* 0.295865* 0.263835* 0.161678 0.259718*
Nucleolus 0.021812 0.074623 0.063485 0.027728 0.020878 0.016539 0.032622 0.100120 0.020949 0.038379 0.035422 0.083243
Nucleus 0.022404 0.073724 0.060805 0.017295 0.011816 0.011735 0.026913 0.125982 0.012616 0.035042 0.029988 0.100644
Peroxisomes 0.000904 0.001539 0.001218 0.000632 0.000583 0.000488 0.000566 0.001118 0.000608 0.000883 0.000625 0.001005
Vacuole 0.011117 0.010133 0.001361 0.002831 0.002537 0.004405 0.001487 0.001908 0.003185 0.006559 0.001476 0.002123
Vacuole Periphery 0.019167 0.018754 0.002286 0.010985 0.009050 0.011842 0.008293 0.002683 0.009815 0.014441 0.007748 0.004618

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.52 -0.74 1.87 10.08 2.73 -0.79 -3.27 -10.31 -5.52 -7.38 -6.46 -3.41 -9.10 -0.30 -5.71
Bud Neck 3.96 2.79 3.77 0.46 1.87 -0.42 -1.88 -8.39 -7.94 -8.17 -1.04 -0.64 -6.30 -6.08 -6.85
Bud Site 1.78 2.10 2.14 3.44 0.55 -1.45 0.21 -4.90 -3.05 -5.09 -0.22 2.06 0.16 0.67 -4.63
Cell Periphery -4.30 2.19 2.96 9.46 2.08 -0.70 0.25 -4.19 -3.21 -3.21 -7.60 0.75 -2.56 5.94 -2.55
Cytoplasm -4.83 0.66 1.94 5.42 1.41 -1.45 -1.62 -2.12 -1.76 -1.73 -5.51 -1.56 -2.08 0.74 -1.62
Cytoplasmic Foci -0.58 3.12 2.39 3.61 -1.96 -1.86 2.88 -1.80 -0.39 -2.73 -4.10 4.11 -1.39 2.40 -3.31
Eisosomes -2.94 0.42 2.14 6.28 1.92 2.13 3.36 -3.45 -5.65 -6.73 -3.57 3.17 -2.32 1.50 -5.72
Endoplasmic Reticulum -7.27 -0.82 1.30 10.29 2.27 -0.41 9.12 -8.56 -7.50 -13.46 -8.70 7.54 -7.53 0.84 -12.52
Endosome 0.24 2.94 3.03 6.01 0.61 -1.29 4.08 -2.57 -2.08 -3.34 -4.18 4.85 -1.81 0.67 -3.40
Golgi 0.97 1.79 1.47 1.63 -1.03 -1.31 1.38 -1.70 -1.14 -2.08 -1.83 2.08 -0.49 1.15 -2.35
Lipid Particles -3.21 3.24 2.41 4.78 -2.30 -1.80 7.68 4.22 4.13 -4.09 -3.70 8.11 4.74 6.26 -4.85
Mitochondria -2.53 1.22 1.39 7.58 0.40 -0.37 2.90 0.52 0.88 -2.53 -2.29 3.10 1.33 4.09 -2.07
Mitotic Spindle 6.64 5.21 6.52 -0.55 2.61 0.24 -2.43 -8.39 -8.47 -7.58 5.03 2.92 -4.23 -8.06 -6.62
None -6.27 0.84 1.10 6.77 1.27 1.07 3.33 -3.04 -3.49 -4.35 -6.24 3.44 -2.42 5.19 -4.14
Nuclear Periphery 2.38 -3.44 -3.74 -10.28 0.22 -8.15 -20.04 17.13 21.87* 29.05* 3.17 -19.32 14.22 11.75 27.45
Nuclear Periphery Foci -4.92 1.99 -4.56 -0.17 -8.16 10.26 32.38* 7.44 -0.01 -13.97 6.61 31.40 5.91 0.85 -17.39
Nucleolus -6.79 -3.74 -0.38 7.78 3.76 2.34 -5.07 -15.77 -16.65 -12.83 -7.77 -6.19 -14.96 -10.39 -10.75
Nucleus -4.63 -2.74 1.62 10.30 4.77 0.05 -7.06 -17.94 -17.84 -14.95 -9.92 -7.70 -16.99 -12.36 -12.92
Peroxisomes -1.31 -0.35 1.26 4.60 2.34 1.95 -0.07 -7.21 -9.06 -7.24 -3.69 -0.40 -5.28 -1.31 -5.15
Vacuole 0.27 2.87 2.47 5.24 -1.03 -3.03 2.76 1.20 3.49 -0.96 -5.42 3.89 1.96 6.72 -1.49
Vacuole Periphery -0.03 3.31 1.38 2.65 -3.36 -2.49 0.90 9.80 8.90 7.74 -4.51 2.34 5.65 9.39 3.57
Standard name
Human Ortholog
Description Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107
Localization
Cell Percentages nuclear periphery (90%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nup84

Nup84


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup84-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available