Standard name
Human Ortholog
Description ATPase dedicated chaperone that adapts proteasome assembly to stress; Tma17p is induced upon stress; interacts with Rpt6p to assist its pairing to Rpt3p and early steps in proteasome biogenesis; associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.96 0.95 0.89 0.61 0.66 0.72 0.67 0.66 0.98 0.99 0.97 0.97 0.98 0.96 0.97 0.92 0.94 0.9 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0 0.05 0.07 0.11 0.11 0.2 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.31 0.21 0.15 0.13 0.16 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 3 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 6 2 0 1 5 3 2 7 3 0 0 1 0 0 0 1 1 0 2 5 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1 1 4 5 8 4 12 6 9 1 0 1 3 2 1 0 0 0 0 0 0
Cytoplasm 451 100 150 200 304 286 329 116 211 166 241 352 497 571 271 247 236 433 98 150 134 366 419
Endoplasmic Reticulum 3 1 1 1 8 0 8 1 13 6 6 3 3 5 5 7 7 0 0 0 2 0 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 2 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 0 0 0 1 1
Mitochondria 4 0 3 12 4 17 40 20 31 49 67 2 1 2 2 1 0 2 0 0 0 0 0
Nucleus 1 2 1 0 1 1 7 2 1 2 4 2 1 2 0 1 0 2 1 1 3 5 2
Nuclear Periphery 0 0 0 0 0 0 4 0 1 2 3 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 7 5 0 0 5 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 0 0 4 10 21 167 36 43 33 58 3 1 11 9 3 9 0 1 2 1 3 3
Unique Cell Count 457 101 152 209 320 320 538 175 292 249 367 359 501 586 280 252 245 447 107 160 149 390 461
Labelled Cell Count 460 104 162 220 331 331 576 188 317 271 398 363 503 594 290 262 253 447 107 160 149 390 461


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 12.6 6.3 7.7 11.5 10.9 12.6 12.3 12.7 12.9 13.0 10.1 12.3 13.9 42.6 41.6 40.0 12.3 11.6 12.2
Std Deviation (1e-4) 1.8 2.8 1.9 1.7 2.5 2.2 3.4 3.6 3.9 4.4 4.5 2.2 2.9 3.4 15.0 15.0 14.2 16.0 8.3 9.7
Intensity Change (Log2) 0.29 0.88 0.8 1.01 0.97 1.02 1.04 1.06 0.69 0.97 1.15 2.76 2.73 2.68 0.98 0.89 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -2.0 -3.6 -8.9 -7.5 -6.8 -7.6 -7.9 -0.5 0.6 -0.9 -1.2 -0.5 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.8 0 1.7 2.5 3.3 3.2 5.1 4.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.2 7.9 5.9 5.0 4.7 5.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.3727 12.7065 9.7302 7.3759 5.9432 10.5318 7.0217 8.1502 7.072 7.1156 6.2341 6.9726 8.0383 10.8394 9.8455 11.0757 9.489 9.218
Actin 0.0244 0.0003 0.0051 0.0397 0.047 0.0003 0.0161 0.0097 0.0049 0.0017 0.0119 0.0022 0.0198 0.002 0.0053 0.0001 0.0003 0.0003
Bud 0.0006 0.0001 0.0002 0.0028 0.0016 0.0002 0.0004 0.0011 0.0006 0.0007 0.0004 0.0003 0.0003 0.001 0.0002 0.0002 0.0002 0.0001
Bud Neck 0.0007 0.0002 0.0008 0.0033 0.0006 0.0012 0.0013 0.0004 0.0009 0.0005 0.0011 0.0014 0.001 0.0002 0.0004 0.0005 0.0006 0.0011
Bud Periphery 0.0004 0 0.0001 0.0031 0.0025 0.0001 0.0004 0.0003 0.0002 0.0003 0.0003 0.0003 0.0002 0.0002 0 0 0 0
Bud Site 0.0009 0.0001 0.0036 0.0185 0.0042 0.0001 0.0014 0.0051 0.0015 0.0002 0.0006 0.0003 0.0006 0.0014 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0002 0 0.0001 0.0003 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.8029 0.8747 0.8597 0.8174 0.5799 0.8734 0.7943 0.921 0.8891 0.9185 0.7716 0.8891 0.7793 0.8466 0.879 0.7375 0.9188 0.8655
Cytoplasmic Foci 0.0267 0.001 0.0032 0.0079 0.0718 0.0016 0.0209 0.0024 0.0094 0.017 0.0336 0.011 0.0153 0.002 0.0018 0.0012 0.005 0.0034
Eisosomes 0.0001 0 0 0.0003 0.0005 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0043 0.0006 0.0011 0.0011 0.0068 0.0016 0.004 0.0011 0.0016 0.0019 0.0039 0.0018 0.003 0.0006 0.001 0.001 0.002 0.0018
Endosome 0.0276 0.0003 0.0008 0.0044 0.071 0.0012 0.0276 0.0006 0.0082 0.0048 0.0551 0.0178 0.0104 0.0003 0.0011 0.0006 0.0015 0.0019
Golgi 0.0038 0 0.0006 0.0078 0.0183 0 0.0108 0.0003 0.0037 0.0028 0.0254 0.0067 0.0019 0 0.0004 0 0.0001 0
Lipid Particles 0.0068 0 0.0004 0.03 0.0202 0 0.0143 0 0.0094 0.0071 0.0157 0.0064 0.0035 0 0.0002 0 0 0
Mitochondria 0.0023 0.0001 0.0002 0.0193 0.0883 0.0003 0.0099 0.0002 0.0009 0.0004 0.0078 0.006 0.0009 0.0002 0.0002 0.0001 0.0002 0.0003
None 0.0034 0.0012 0.0034 0.0009 0.0028 0.0011 0.005 0.0056 0.0039 0.0029 0.0029 0.0022 0.023 0.0057 0.0022 0.002 0.0014 0.0016
Nuclear Periphery 0.0112 0.003 0.0028 0.0017 0.0224 0.0084 0.0177 0.0024 0.0038 0.0033 0.031 0.0037 0.006 0.0022 0.0039 0.0042 0.0047 0.0065
Nucleolus 0.0004 0 0.0001 0.0002 0.0006 0.0002 0.0002 0.0001 0.0001 0 0.0003 0.0001 0.0003 0.0001 0 0.0001 0.0001 0.0001
Nucleus 0.0556 0.1171 0.1168 0.0108 0.0149 0.1078 0.0504 0.0476 0.0547 0.01 0.0251 0.0462 0.1164 0.1356 0.1019 0.2502 0.0615 0.1139
Peroxisomes 0.0021 0 0.0003 0.0285 0.0349 0 0.0192 0.0004 0.0045 0.025 0.0044 0.0013 0.0059 0.0001 0.0001 0 0.0001 0
Punctate Nuclear 0.0098 0.0009 0.0006 0.0008 0.0034 0.001 0.0025 0.0014 0.0015 0.0017 0.0025 0.0014 0.0109 0.0012 0.0012 0.0009 0.0026 0.0024
Vacuole 0.0134 0.0002 0.0002 0.001 0.0043 0.0012 0.0022 0.0004 0.0008 0.0009 0.0032 0.0012 0.0009 0.0004 0.0005 0.0011 0.0006 0.0006
Vacuole Periphery 0.0024 0 0.0001 0.0003 0.0036 0.0003 0.0013 0.0001 0.0003 0.0002 0.0031 0.0007 0.0004 0.0001 0.0001 0.0002 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.4862 30.484 11.3102 24.8375 33.1599 65.7294 33.5744 47.5498 53.5194 28.7485
Translational Efficiency 2.9848 1.9172 4.2879 2.7263 1.4886 2.0804 2.6031 2.4517 2.4358 2.4505

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1333 459 118 1854 1294 1830 823 1514 2627 2289 941 3368

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 832.40 1105.97 2113.97 1439.68 1001.15 1006.05 1497.28 1332.19 915.52 1026.09 1574.61 1391.36
Standard Deviation 170.06 251.98 432.84 420.77 171.94 160.13 320.03 351.29 190.67 186.64 393.42 394.71
Intensity Change Log 2 0.409963 1.344606 0.790399 0.007044 0.580686 0.412142 0.203984 0.977859 0.596220

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000145 0.000807 0.000765 0.001153 0.000436 0.000293 0.000289 0.000652 0.000288 0.000396 0.000348 0.000928
Bud Neck 0.002520 0.005407 0.005073 0.004544 0.002828 0.003030 0.002408 0.003929 0.002672 0.003507 0.002742 0.004267
Bud Site 0.005156 0.017483 0.006127 0.015700 0.008806 0.007627 0.009985 0.021158 0.006954 0.009604 0.009501 0.018153
Cell Periphery 0.000249 0.000214 0.000664 0.000079 0.000177 0.000069 0.000169 0.000086 0.000213 0.000098 0.000231 0.000082
Cytoplasm 0.742641* 0.624421* 0.477499* 0.686230* 0.704602* 0.760162* 0.687868* 0.712710* 0.723904* 0.732942* 0.661488* 0.698133*
Cytoplasmic Foci 0.082971 0.069582 0.019923 0.008583 0.074144 0.069835 0.007599 0.020543 0.078623 0.069785 0.009144 0.013960
Eisosomes 0.000028 0.000036 0.000013 0.000009 0.000030 0.000020 0.000005 0.000015 0.000029 0.000023 0.000006 0.000012
Endoplasmic Reticulum 0.001267 0.001944 0.000773 0.000359 0.001852 0.000675 0.000304 0.000511 0.001555 0.000930 0.000363 0.000427
Endosome 0.003675 0.008507 0.001101 0.007682 0.003633 0.006794 0.000867 0.012554 0.003654 0.007138 0.000896 0.009872
Golgi 0.000989 0.006576 0.000521 0.001535 0.000747 0.003705 0.000261 0.005610 0.000870 0.004281 0.000293 0.003367
Lipid Particles 0.001784 0.001308 0.001877 0.000631 0.001333 0.000760 0.000551 0.001418 0.001562 0.000870 0.000717 0.000985
Mitochondria 0.000634 0.002438 0.000631 0.001383 0.001803 0.001561 0.000285 0.014991 0.001209 0.001737 0.000329 0.007500
Mitotic Spindle 0.002675 0.041328 0.009399 0.109362* 0.008449 0.009911 0.023748 0.052438 0.005519 0.016211 0.021949 0.083774
None 0.005604 0.004673 0.005306 0.008814 0.002098 0.002537 0.012937 0.004925 0.003877 0.002966 0.011980 0.007066
Nuclear Periphery 0.000331 0.000454 0.001326 0.000540 0.000381 0.000201 0.000411 0.000325 0.000356 0.000252 0.000526 0.000443
Nuclear Periphery Foci 0.001641 0.003028 0.002862 0.001077 0.001917 0.001449 0.001670 0.002351 0.001777 0.001766 0.001819 0.001650
Nucleolus 0.000424 0.001428 0.000218 0.000600 0.000247 0.000453 0.000163 0.000392 0.000337 0.000649 0.000170 0.000506
Nucleus 0.107766 0.122074 0.396735* 0.081296 0.121574 0.073793 0.215802* 0.058825 0.114568 0.083475 0.238491* 0.071195
Peroxisomes 0.000536 0.002827 0.000127 0.001111 0.000715 0.001252 0.000407 0.002232 0.000624 0.001568 0.000372 0.001615
Vacuole 0.038035 0.079975 0.068878 0.064518 0.063039 0.055036 0.034133 0.079004 0.050351 0.060037 0.038490 0.071030
Vacuole Periphery 0.000929 0.005491 0.000184 0.004793 0.001187 0.000834 0.000139 0.005334 0.001056 0.001768 0.000145 0.005036

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.43 -7.72 -2.55 1.55 4.53 0.68 -0.49 -0.03 -0.68 0.42 -1.50 -3.86 -1.59 -0.25 1.48
Bud Neck -2.37 -7.31 -1.25 1.94 6.97 -0.11 -1.33 1.56 1.53 2.59 -1.75 -4.24 -0.01 1.82 4.36
Bud Site -4.10 -5.18 -2.71 3.10 3.14 0.77 -0.37 -1.41 -2.40 -0.83 -2.36 -2.01 -2.55 0.20 0.42
Cell Periphery 0.52 -5.70 1.73 2.94 7.97 4.16 -1.52 3.37 -2.65 7.73 2.44 -2.52 2.61 0.73 10.38
Cytoplasm 10.37 20.49 16.88 -0.57 -13.50 -7.41 9.90 5.28 13.26 -5.15 -0.77 17.89 15.39 14.90 -7.28
Cytoplasmic Foci 3.00 12.80 23.97 11.77 6.34 0.75 20.39 19.30 19.95 -2.74 2.87 28.14 30.87 24.63 2.67
Eisosomes -2.06 0.03 11.83 6.43 6.44 3.71 7.40 6.76 6.40 -2.01 3.05 10.28 11.95 9.71 2.38
Endoplasmic Reticulum -2.51 -0.02 4.93 7.73 6.36 5.13 6.19 7.16 5.46 4.53 3.66 6.34 8.61 9.24 7.68
Endosome -3.18 4.88 3.30 4.59 0.21 -3.62 8.67 0.83 3.62 -3.57 -4.47 11.13 3.00 5.78 -3.32
Golgi -2.22 0.73 2.79 2.58 4.03 -3.31 3.06 -1.20 2.48 -2.44 -3.83 4.13 0.46 3.91 -2.36
Lipid Particles 0.48 -3.41 0.88 1.73 6.88 2.12 -0.79 0.42 -2.50 1.75 1.69 -1.00 0.99 -2.55 5.18
Mitochondria -1.93 -0.55 0.28 2.32 2.56 0.22 1.69 -1.08 -1.41 -2.50 -0.81 1.77 -0.86 -0.23 -2.58
Mitotic Spindle -4.75 -2.49 -13.95 -2.84 -11.03 -0.50 -4.19 -7.02 -6.76 -2.07 -4.33 -5.40 -15.05 -9.99 -5.97
None 1.26 1.95 -4.14 -4.78 -7.37 -1.43 -13.38 -8.79 -7.54 8.03 2.57 -9.26 -7.81 -12.96 4.63
Nuclear Periphery -1.96 -9.12 -5.40 -6.91 7.14 5.92 -8.70 -3.96 -14.06 6.62 1.50 -9.31 -6.58 -19.68 5.80
Nuclear Periphery Foci -4.47 -5.42 2.84 5.83 6.48 1.39 -2.36 0.36 -0.87 2.45 -0.82 -4.69 1.97 2.36 6.21
Nucleolus -1.36 1.23 0.71 1.58 -0.87 -0.95 0.80 0.77 1.17 0.11 -1.13 1.98 0.84 1.50 -1.59
Nucleus -2.57 -13.81 -4.62 -0.04 12.83 10.34 -14.24 7.82 -3.46 21.90 8.34 -20.17 2.10 -7.60 22.17
Peroxisomes -2.20 4.62 1.71 2.50 -0.66 -2.08 2.52 0.27 2.07 -1.71 -3.40 3.46 1.17 3.92 -1.64
Vacuole -9.45 -8.03 -24.24 -5.32 1.12 3.14 -4.99 -17.22 -20.45 -9.87 -4.95 -10.25 -28.75 -23.11 -8.97
Vacuole Periphery -1.80 1.96 0.01 1.79 -1.70 0.73 2.53 -0.05 -0.76 -2.49 -1.02 3.45 -0.01 0.99 -3.19