Standard name
Description Serine/threonine protein kinase, subunit of transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters; phosphorylates Ser5 residue of the PolII C-terminal domain (CTD) at gene promoters; relocalizes to the cytosol in response to hypoxia

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0 0.06 0 0 0 0 0.05 0 0 0.10 0.12 0.10 0.05 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0 0 0.12 0.23 0.22 0.39 0.34 0.52 0.27 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0.93 0.93 0.96 0.92 0.95 0.89 0.83 0.85 0.81 0.85 0.75 0.85 0.94 0.88 0.85 0.81 0.87 0.87 0.90 0.87 0.89 0.84 0.78 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.18 0.06 0 0 0.05 0.12 0.07 0.15 0.12 0.19 0.09 0 0 0 0 0 0.08 0.07 0.07 0.05 0 0.09 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.10 0 0 0 0 0.05 0.08 0.10
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 3 0 3 3 4 1 0 0 0 0 0 0 0 1 1 0 2 1
Bud Neck 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 0 0 3 4 6 12 7 14 13 10 0 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 2 14 13 21 23 16 5 5 15 6 12 35 61 13 3 4 0 0 1 1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 1 0 0 2 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1
Mitochondria 43 2 8 2 4 47 120 95 135 121 153 123 2 0 0 1 3 4 1 0 0 1 8 7
Nucleus 257 92 183 180 315 338 433 364 283 298 219 389 261 305 416 110 54 72 245 90 184 172 327 322
Nuclear Periphery 0 0 0 3 0 2 7 2 6 1 5 2 0 3 3 1 0 0 0 0 0 1 0 1
Nucleolus 46 18 12 6 10 18 62 32 54 42 56 41 9 7 22 6 2 7 19 6 9 8 37 50
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 3 0 5 3 1 2 1 6 2 5 5 0 0 0 0 0 1 1 2 3
Vac/Vac Membrane 0 0 1 1 2 0 0 6 0 3 2 1 1 1 7 8 6 3 3 3 5 10 31 44
Unique Cell Count 276 99 191 196 333 379 521 429 349 351 292 457 279 345 489 135 62 83 273 104 207 204 419 442
Labelled Cell Count 349 115 207 207 350 430 661 530 494 489 469 579 288 356 518 143 68 92 273 104 207 204 419 442

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 8.5 6.8 6.5 6.8 5.7 5.1 5.4 4.6 4.6 4.1 4.9 7.3 7.5 7.5 11.2 10.1 10.0 7.0 7.2 7.9
Std Deviation (1e-4) 0.8 1.5 1.3 1.4 1.4 1.7 1.4 1.3 1.3 1.1 1.3 1.0 1.2 1.6 1.8 2.8 3.0 2.8 1.8 1.4 1.4
Intensity Change (Log2) -0.06 -0.0 -0.25 -0.4 -0.32 -0.58 -0.55 -0.73 -0.45 0.1 0.15 0.15 0.72 0.58 0.56 0.04 0.09 0.22

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 5.8 5.5 0 0 0 6.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 3.3 4.4 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -1.8 -0.8 2.2 0.5 0 0 0 1.1 -1.6 -2.6 -1.0 -0.7 0 0.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0