Standard name
Human Ortholog
Description Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.11 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0 0 0 0 0.15 0.09 0.34 0.12 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0.94 0.93 0.97 0.96 0.95 0.92 0.93 0.92 0.87 0.89 0.78 0.85 0.92 0.88 0.83 0.93 0.9 0.86 0.9 0.91 0.9 0.83 0.78 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0.06 0.11 0.07 0.13 0.12 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0.05 0.1 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 0 1 2 1 0 0 0 0 1 1 1 2 1 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 0 2 10 6 4 0 0 0 0 1 1 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 12 2 8 14 11 20 13 10 8 14 4 10 24 35 5 5 19 1 1 0 0 5 2
Endoplasmic Reticulum 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 2 4 3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 1 2 1 0 0 0 2 1 8 1 2 6 0 1 0 0 0 0
Golgi 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 2 1 1 1 0 0 0 0 0
Mitochondria 20 5 1 1 1 12 15 21 41 32 112 58 0 2 2 10 17 11 3 4 1 0 1 4
Nucleus 210 316 138 247 326 332 528 459 240 311 252 413 141 217 263 250 266 321 203 314 135 44 120 151
Nuclear Periphery 1 1 0 0 0 1 5 1 3 3 1 2 2 4 6 4 2 2 0 0 0 0 0 0
Nucleolus 18 7 0 1 3 10 12 32 31 26 41 56 4 4 9 6 10 6 7 6 2 1 5 16
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0 0 0 0
SpindlePole 1 1 1 0 1 1 1 4 0 3 1 3 0 2 1 3 0 3 1 0 1 0 0 5
Vac/Vac Membrane 1 9 1 1 4 7 10 3 3 4 3 15 0 2 8 13 10 25 5 8 5 2 15 33
Unique Cell Count 224 338 142 258 344 359 565 498 277 350 325 486 154 246 318 269 296 372 227 344 151 54 155 222
Labelled Cell Count 256 353 143 260 349 374 596 536 331 398 433 557 159 257 334 296 319 400 227 344 151 54 155 222


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.0 7.3 7.2 8.0 6.7 6.7 6.7 5.8 6.1 5.1 5.9 8.2 8.0 7.8 8.8 8.6 8.3 7.4 7.7 8.3
Std Deviation (1e-4) 0.8 1.2 1.0 1.5 1.4 1.4 1.3 1.2 1.3 1.3 1.3 1.3 2.4 2.1 2.9 2.1 2.1 1.9 1.1 2.3 3.1
Intensity Change (Log2) – – – -0.02 0.13 -0.14 -0.13 -0.13 -0.34 -0.26 -0.54 -0.32 0.15 0.12 0.08 0.26 0.23 0.18 0.0 0.06 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.2 3.2 3.5 0 0 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.5 3.4 7.8 4.0 0 0 0 0 2.5 0
Nucleus -0.7 -1.2 -2.0 -1.7 -2.1 -3.4 -3.0 -5.3 -3.9 -2.1 -3.1 -4.3 -1.8 -2.7 -3.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 3.1 4.1 3.3 4.4 4.2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6772 3.7881 3.4542 3.3398 2.8167 3.2672 2.4285 3.1534 2.6463 1.9257 2.4146 2.3573 2.3146 2.219 1.9811 1.9717 2.3353 1.8314
Actin 0 0 0 0 0 0 0.0114 0 0.0109 0.0006 0.006 0.0014 0.0167 0 0.0052 0.0063 0.0284 0.0022
Bud 0 0 0 0 0 0 0.0004 0 0.0003 0 0.0034 0.0001 0.0003 0 0.0004 0.0006 0.0005 0.0001
Bud Neck 0 0 0 0 0.0002 0.0005 0.0008 0 0.0005 0.0002 0.0022 0.0032 0.0005 0 0.0002 0.0002 0.0006 0.0008
Bud Periphery 0 0 0 0 0 0 0.0006 0 0.0007 0.0001 0.0063 0.0004 0.0006 0 0.0004 0.0013 0.0014 0.0003
Bud Site 0 0 0 0 0 0 0.0031 0 0.0027 0.0001 0.0215 0.0002 0.0014 0 0.0029 0.0008 0.0013 0.0002
Cell Periphery 0 0 0 0 0 0 0.0003 0 0.0002 0 0.0023 0.0001 0.0002 0 0.0002 0.0002 0.0005 0.0001
Cytoplasm 0 0 0 0 0 0 0.0028 0 0.0007 0.0009 0.0019 0.0004 0.0157 0 0.0089 0.0051 0.0005 0.0003
Cytoplasmic Foci 0 0 0 0 0 0 0.0172 0 0.0065 0.03 0.0124 0.0024 0.0082 0 0.0062 0.0028 0.0092 0.0062
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0004 0.0004 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0009 0 0.0005 0.0002 0.0008 0.0005 0.0003 0 0.0015 0.0013 0.001 0.0002
Endosome 0 0 0 0 0 0 0.0137 0 0.0062 0.0051 0.0174 0.0106 0.0066 0 0.0073 0.0047 0.0084 0.0079
Golgi 0 0 0 0 0 0 0.0054 0 0.0036 0.002 0.0153 0.0069 0.0061 0 0.0031 0.0051 0.0165 0.0047
Lipid Particles 0 0 0 0 0 0 0.0205 0 0.0069 0.0168 0.0278 0.0025 0.018 0 0.0016 0.0068 0.0308 0.0021
Mitochondria 0.0001 0 0 0 0.0001 0 0.0042 0.0001 0.0058 0.0004 0.0272 0.0057 0.0062 0.0001 0.005 0.1479 0.0773 0.0041
None 0 0 0 0 0 0 0.0197 0 0.0003 0.0003 0.0009 0.0003 0.0015 0 0.0005 0.0445 0.0005 0.0001
Nuclear Periphery 0.0001 0.0002 0.0003 0.0002 0.0002 0.0001 0.0059 0.0002 0.0054 0.0006 0.0044 0.0058 0.0021 0.0002 0.0006 0.0011 0.0248 0.0033
Nucleolus 0.0108 0.0129 0.0087 0.0054 0.0424 0.0346 0.0144 0.0055 0.0131 0.0038 0.0746 0.0407 0.0141 0.0094 0.0094 0.0042 0.0667 0.0346
Nucleus 0.9888 0.9867 0.9909 0.9943 0.956 0.9645 0.8541 0.994 0.9196 0.9052 0.7406 0.9134 0.882 0.9901 0.9435 0.7606 0.6979 0.9294
Peroxisomes 0 0 0 0 0 0 0.0047 0 0.0107 0.0241 0.0071 0.0012 0.0152 0 0.0014 0.0021 0.0217 0.0005
Punctate Nuclear 0.0001 0.0002 0.0001 0.0001 0.0008 0.0001 0.0162 0.0001 0.0032 0.0096 0.0026 0.0021 0.0026 0.0001 0.0006 0.0011 0.0012 0.0005
Vacuole 0 0 0 0 0 0 0.0022 0 0.0016 0.0001 0.0198 0.0013 0.0008 0 0.0008 0.0011 0.0033 0.001
Vacuole Periphery 0 0 0 0 0 0 0.0012 0 0.0008 0 0.0055 0.0005 0.0005 0 0.0003 0.002 0.0072 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.195 7.0455 1.7427 11.4178 8.5155 7.2207 10.6856 11.0184 11.0848 14.2466
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
442 1559 207 1189 1604 2131 300 2814 2046 3690 507 4003

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 731.76 745.53 1211.49 1175.33 819.06 823.62 1101.61 1206.22 800.20 790.63 1146.47 1197.04
Standard Deviation 92.02 163.05 134.76 170.60 106.48 120.46 113.08 168.07 109.59 145.26 133.78 169.41
Intensity Change Log 2 — 0.026896 0.727340 0.683623 — 0.008010 0.427573 0.558452 — 0.016952 0.576796 0.618870

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000133 0.000521 0.002153 0.000960 0.000317 0.000311 0.001222 0.000404 0.000277 0.000400 0.001602 0.000569
Bud Neck 0.001149 0.006470 0.016222 0.022113 0.004337 0.007931 0.005791 0.033268 0.003648 0.007314 0.010050 0.029955
Bud Site 0.000175 0.001019 0.002149 0.004634 0.000695 0.000970 0.001156 0.002349 0.000582 0.000991 0.001561 0.003028
Cell Periphery 0.000062 0.000250 0.000079 0.000074 0.000066 0.000142 0.000088 0.000066 0.000065 0.000188 0.000085 0.000069
Cytoplasm 0.003649 0.004720 0.000373 0.001836 0.002207 0.000988 0.001443 0.001578 0.002519 0.002565 0.001006 0.001655
Cytoplasmic Foci 0.000185 0.000250 0.000066 0.001035 0.000533 0.000378 0.000195 0.000170 0.000458 0.000324 0.000142 0.000427
Eisosomes 0.000012 0.000021 0.000080 0.000035 0.000018 0.000020 0.000053 0.000029 0.000016 0.000020 0.000064 0.000030
Endoplasmic Reticulum 0.001337 0.004059 0.005950 0.003355 0.002905 0.003560 0.007215 0.002365 0.002566 0.003771 0.006699 0.002659
Endosome 0.000206 0.000403 0.000197 0.002342 0.000312 0.000853 0.000212 0.000430 0.000289 0.000663 0.000206 0.000998
Golgi 0.000056 0.000096 0.000155 0.002873 0.000349 0.000316 0.000095 0.000466 0.000286 0.000223 0.000120 0.001181
Lipid Particles 0.000204 0.000114 0.000199 0.000132 0.000272 0.000196 0.000254 0.000023 0.000257 0.000161 0.000232 0.000056
Mitochondria 0.000336 0.001022 0.002152 0.002976 0.001912 0.002320 0.000912 0.002186 0.001571 0.001771 0.001419 0.002421
Mitotic Spindle 0.002024 0.000972 0.000247 0.016672 0.002684 0.003143 0.001003 0.006062 0.002541 0.002226 0.000695 0.009213
None 0.006468 0.018232 0.000881 0.001571 0.002542 0.001872 0.002979 0.001401 0.003390 0.008784 0.002122 0.001452
Nuclear Periphery 0.000165 0.000330 0.000362 0.001476 0.000417 0.001094 0.000334 0.001254 0.000363 0.000771 0.000346 0.001320
Nuclear Periphery Foci 0.000620 0.000337 0.000091 0.001662 0.000233 0.000738 0.000180 0.000158 0.000317 0.000568 0.000144 0.000605
Nucleolus 0.026901 0.017612 0.014851 0.010081 0.020195 0.016465 0.011867 0.005311 0.021643 0.016950 0.013085 0.006728
Nucleus 0.955869* 0.942278* 0.949536* 0.918826* 0.958824* 0.955164* 0.961885* 0.940780* 0.958186* 0.949719* 0.956843* 0.934259*
Peroxisomes 0.000170 0.000229 0.001167 0.000279 0.000664 0.000271 0.000432 0.000056 0.000557 0.000253 0.000732 0.000122
Vacuole 0.000183 0.000612 0.002889 0.005541 0.000335 0.001777 0.002566 0.001239 0.000302 0.001285 0.002698 0.002517
Vacuole Periphery 0.000097 0.000453 0.000201 0.001527 0.000183 0.001490 0.000116 0.000407 0.000164 0.001052 0.000151 0.000739

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -11.38 -15.30 -18.70 -11.21 8.18 0.14 -13.30 -7.92 -8.77 10.76 -4.45 -18.94 -16.33 -12.95 13.49
Bud Neck -7.94 -11.84 -19.50 -14.52 -5.53 -5.38 -4.24 -28.30 -23.77 -20.46 -7.36 -10.14 -33.36 -28.00 -19.40
Bud Site -5.27 -12.09 -4.52 -3.57 -1.52 -0.69 -2.44 -3.75 -3.50 -2.27 -1.31 -5.11 -5.44 -5.13 -2.34
Cell Periphery -2.80 -1.45 -1.08 2.56 1.05 -0.81 -0.74 0.04 0.84 0.97 -2.00 -1.25 -0.41 1.95 1.36
Cytoplasm -0.67 1.93 1.04 4.54 -4.39 1.47 0.05 0.82 -1.13 0.50 -0.26 1.36 1.27 2.52 -0.52
Cytoplasmic Foci -0.96 1.13 -1.59 -1.08 -2.20 0.77 1.72 2.34 2.29 1.00 0.75 2.08 1.47 1.13 -0.82
Eisosomes -5.16 -14.82 -16.43 -8.27 9.02 -1.11 -12.09 -8.34 -6.61 9.26 -2.82 -18.03 -13.87 -9.89 13.17
Endoplasmic Reticulum -10.04 -10.73 -10.25 0.97 6.00 -2.57 -11.13 2.37 4.55 12.29 -6.12 -14.31 -2.30 4.91 13.76
Endosome -1.52 -0.84 -2.78 -2.28 -2.64 -2.32 -0.11 -0.02 2.27 0.07 -2.54 -0.26 -2.23 0.20 -2.30
Golgi -2.04 -6.42 -1.99 -1.94 -1.79 0.10 0.92 0.09 0.01 -0.89 0.30 0.65 -1.26 -1.68 -1.95
Lipid Particles 1.08 -1.14 0.69 -1.13 3.30 0.71 -0.69 3.33 3.05 7.18 1.30 -0.90 3.23 3.00 8.26
Mitochondria -4.91 -15.43 -7.49 -5.46 -1.91 -0.51 1.11 -0.79 -0.30 -4.52 -0.35 -0.21 -1.95 -2.56 -4.20
Mitotic Spindle 0.52 0.77 -4.67 -5.78 -6.00 -0.33 0.52 -2.92 -2.91 -3.82 0.27 1.07 -5.31 -6.40 -7.17
None -5.04 4.31 3.29 8.15 -5.66 1.74 -0.57 2.21 -0.11 1.05 -5.84 1.16 3.86 7.90 0.45
Nuclear Periphery -4.71 -7.19 -5.92 -5.20 -4.45 -1.87 -1.05 -4.43 -0.67 -4.15 -1.93 -2.32 -6.39 -2.57 -5.72
Nuclear Periphery Foci 0.56 0.91 -0.83 -2.25 -2.75 -1.91 -0.80 1.53 2.30 1.24 -1.35 0.01 -0.70 0.81 -0.64
Nucleolus 3.14 3.00 3.45 0.84 0.17 2.67 2.67 6.10 3.64 0.78 3.77 3.65 6.73 3.75 1.01
Nucleus 2.48 2.71 7.06 5.96 5.42 1.00 1.86 9.76 8.34 4.01 2.84 3.26 12.18 9.92 6.18
Peroxisomes -1.49 -8.41 -4.03 -2.68 6.98 1.51 0.73 2.43 2.11 8.57 1.54 -1.29 2.10 1.67 10.28
Vacuole -3.59 -6.51 -4.95 -4.44 -0.06 -3.19 -2.79 -4.96 0.58 2.35 -3.72 -3.92 -6.71 -2.87 2.49
Vacuole Periphery -1.39 -4.59 -2.92 -1.25 -2.42 -2.85 1.00 -1.37 2.56 -2.08 -3.09 -0.52 -3.08 1.86 -3.05
Standard name
Human Ortholog
Description Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Pho2

Pho2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available