Standard name
Description Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.08 0.05 0.09 0.08 0.07 0 0.05 0.09 0.14 0.2 0.13 0.11 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0 0 0.15 0.11 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.85 0.85 0.83 0.47 0.45 0.48 0.44 0.47 0.46 0.43 0.4 0.81 0.69 0.58 0.55 0.52 0.49 0.83 0.81 0.62 0.82 0.71 0.51
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 0 0 0 0.05 0 0 0.06 0.05 0.13 0.08 0 0 0 0 0 0 0.09 0.1 0.24 0.05 0.08 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.11 0.1 0.12 0.49 0.4 0.38 0.38 0.33 0.27 0.3 0.31 0.09 0.2 0.21 0.29 0.32 0.35 0.05 0.05 0.08 0.08 0.14 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 1 1 1 1 0 1 0 1 0 0 0 0 0 0
Bud 0 0 0 0 1 1 4 0 2 3 1 2 0 0 1 0 1 0 0 2 7 2 5 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 1 1 4 1 5 4 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 0 6 10 7 8 15 15 26 18 17 8 10 23 36 71 31 29 22 0 0 0 4 3 5
Endoplasmic Reticulum 0 0 0 0 0 1 0 2 1 2 0 1 0 0 1 6 11 6 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 1 1 1 1 0 2 1 5 0 0 0 0 1 1 3 2 8
Golgi 0 0 0 0 0 3 0 5 5 1 1 0 1 2 4 9 6 4 0 0 2 0 0 3
Mitochondria 0 0 0 0 0 7 16 9 9 37 22 33 1 0 0 2 6 2 0 1 0 1 1 4
Nucleus 33 257 233 150 100 89 134 121 101 118 87 78 219 181 208 132 132 91 56 264 209 261 223 237
Nuclear Periphery 0 0 4 4 4 3 9 5 2 6 4 7 1 3 2 2 2 0 0 0 4 0 2 5
Nucleolus 1 18 5 3 5 9 9 12 12 13 27 16 3 1 3 6 10 5 5 33 81 15 24 63
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 2 2
Vac/Vac Membrane 4 34 27 22 103 79 106 105 71 70 60 62 23 52 76 69 82 64 3 15 26 25 44 118
Unique Cell Count 36 301 274 181 211 196 278 274 213 255 201 197 269 261 356 241 255 184 68 325 339 318 315 462
Labelled Cell Count 38 315 279 186 221 208 294 290 224 273 216 214 274 278 373 258 279 195 68 325 339 318 315 462


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.3 29.0 23.2 21.1 21.2 20.0 19.5 18.9 18.5 17.4 16.4 14.6 24.6 25.4 22.1 27.5 23.0 26.6 24.9 23.8 26.0
Std Deviation (1e-4) 3.4 6.0 5.8 4.5 4.5 4.2 4.2 5.1 5.1 5.2 4.7 4.9 6.9 7.8 6.7 8.8 7.6 8.7 6.2 6.6 7.7
Intensity Change (Log2) – – – -0.14 -0.13 -0.22 -0.25 -0.3 -0.32 -0.42 -0.5 -0.66 0.08 0.13 -0.07 0.25 -0.01 0.2 0.1 0.04 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.1 1.9 1.0 2.8 2.3 1.6 0.2 0.8 2.4 4.2 6.1 3.9 3.4 3.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.0 0 0 6.5 5.6 7.0 0 0 0 0 0 0
Nucleus -0.6 -8.9 -9.1 -9.2 -10.0 -8.9 -9.4 -9.6 -10.3 -1.1 -4.3 -7.2 -7.5 -8.3 -8.2
Nuclear Periphery 0 0 0 1.4 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.7 1.1 1.7 2.3 2.1 5.0 3.3 0 0 0 0.5 1.5 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.8 9.6 7.8 7.8 7.8 6.4 5.2 5.6 5.9 -0.5 3.3 3.9 5.5 6.3 6.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 24.2761 26.1751 24.4194 23.6228 24.1004 24.0111 17.7138 29.5056 25.6913 23.1221 21.4815 23.4922 23.816 31.4337 28.933 27.6683 27.8896 28.6116
Actin 0.0001 0 0 0 0.0001 0.0001 0.0348 0.0001 0.0146 0.0018 0.0001 0.0033 0.0065 0.0016 0.0027 0.0005 0.0001 0.0046
Bud 0.0001 0 0 0 0.0001 0.0002 0.0012 0.0001 0.0004 0.0001 0.0003 0.0011 0.0005 0.002 0.0005 0.0001 0.0001 0.0001
Bud Neck 0 0 0.0001 0.0001 0.0003 0.0005 0.0007 0 0.0001 0.0001 0.0003 0.0021 0.0004 0.0001 0.0001 0.0001 0.0002 0.0006
Bud Periphery 0.0001 0 0 0 0.0001 0.0004 0.0017 0 0.0004 0.0001 0.0003 0.0026 0.0007 0.001 0.0005 0.0001 0.0001 0.0003
Bud Site 0 0 0 0 0 0.0001 0.0027 0.0001 0.0005 0.0002 0.0001 0.0007 0.0012 0.0014 0.0011 0.0001 0 0.0001
Cell Periphery 0.0001 0 0.0001 0 0 0.0003 0.0007 0.0001 0.0003 0 0.0002 0.0012 0.0005 0.0001 0.0002 0.0002 0 0.0002
Cytoplasm 0.0007 0.001 0.0004 0.0002 0.0003 0.0008 0.0369 0.0029 0.0018 0.0027 0.0011 0.0086 0.0219 0.0026 0.0071 0.0051 0.0003 0.0018
Cytoplasmic Foci 0 0.0008 0.0007 0 0 0 0.0217 0 0.0061 0.0005 0 0.0021 0.0185 0.0012 0.004 0.0072 0 0.0003
Eisosomes 0 0 0.0001 0 0 0 0.0004 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.0002 0.0101 0 0.0002 0.0008 0.0001 0.0041 0.0032 0.0003 0.0005 0.0007 0 0.0004
Endosome 0 0 0.0002 0 0.0001 0.0002 0.0261 0 0.0006 0.0018 0.0001 0.031 0.0243 0.0014 0.0036 0.0006 0 0.0015
Golgi 0 0 0 0 0 0 0.0063 0 0.0005 0.0001 0 0.0037 0.0026 0.0002 0.0005 0.0003 0 0.0009
Lipid Particles 0 0.0002 0.0007 0 0 0.0001 0.0092 0 0.0016 0.0001 0 0.0024 0.0083 0.0002 0.0006 0.0026 0 0.0005
Mitochondria 0.0009 0.0005 0.0005 0.0004 0.0012 0.0004 0.0121 0.0007 0.0028 0.0015 0.0007 0.0043 0.0046 0.0007 0.0052 0.0016 0.0006 0.0061
None 0 0.0001 0.0101 0 0 0 0.0114 0.0001 0.0012 0.0005 0.0001 0.0005 0.0039 0.0002 0.0005 0.0041 0 0.0002
Nuclear Periphery 0.0148 0.0157 0.0079 0.0041 0.0071 0.0062 0.0749 0.0065 0.004 0.0377 0.0035 0.0137 0.0407 0.0202 0.0101 0.018 0.0148 0.0116
Nucleolus 0.0073 0.0147 0.0099 0.0061 0.0407 0.0172 0.0158 0.004 0.0172 0.0024 0.0352 0.0334 0.0545 0.0078 0.0077 0.0379 0.0232 0.0176
Nucleus 0.9732 0.9656 0.9619 0.9887 0.9484 0.9697 0.6492 0.9848 0.9358 0.9392 0.9543 0.8651 0.7598 0.9561 0.9482 0.9047 0.9595 0.9481
Peroxisomes 0 0 0 0 0 0 0.0075 0 0.0031 0.0001 0 0.0002 0.0024 0.0001 0.0006 0.0001 0 0.0001
Punctate Nuclear 0.0002 0.0006 0.0014 0.0001 0.0006 0.0002 0.0632 0.0001 0.0047 0.0097 0.0005 0.0051 0.029 0.0021 0.0032 0.0132 0.0003 0.0008
Vacuole 0.0009 0.0002 0.0046 0.0001 0.0003 0.0026 0.0072 0.0002 0.0021 0.0003 0.0018 0.0081 0.0077 0.0003 0.0015 0.0007 0.0003 0.0016
Vacuole Periphery 0.0015 0.0003 0.0012 0.0001 0.0006 0.0009 0.0065 0.0002 0.0022 0.0005 0.0012 0.0069 0.0086 0.0005 0.0015 0.002 0.0005 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 99.3487 92.2129 88.0564 116.6405 89.3447 109.8568 100.3056 118.6353 118.603 107.4355
Translational Efficiency 2.4868 1.9371 2.3097 1.8437 2.199 2.414 1.9612 1.7047 1.8751 1.7421

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘