Standard name
Human Ortholog
Description Clathrin-coated vesicle component, regulator of endocytosis; copurifies with the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; required for efficient clathrin-mediated endocytosis; ortholog of fission yeast Ucp10

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.07 0 0.07 0.07 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.79 0.82 0.8 0.73 0.72 0.66 0.56 0.62 0.57 0.56 0.49 0.55 0.93 0.97 0.96 0.44 0.67 0.52 0.25 0.2 0.12 0.37 0.36 0.27
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0.1 0.11 0.11 0 0 0.12 0.07 0 0 0 0 0 0 0.11 0 0.05 0.16 0.16 0.23 0.06 0.06 0.09
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.07 0.06 0.13 0.16 0.3 0.44 0.28 0.4 0.52 0.58 0.51 0 0 0 0 0 0 0.26 0.16 0.32 0.23 0.19 0.18
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 0.06 0 0.07 0.13 0.1 0.06 0.08 0.08 0.08 0.1 0 0 0.07 0.62 0.45 0.53 0.23 0.37 0.2 0.26 0.3 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 2 2 2 3 4 0 1 0 0 1 4 1 0 0 0 0 4 2 12 5 4 6
Bud 3 3 9 10 18 13 57 32 43 59 60 60 1 0 0 1 0 0 1 6 5 3 6 14
Bud Neck 1 0 0 0 1 0 1 0 0 1 0 1 1 2 1 0 0 0 1 1 1 0 1 3
Bud Site 0 0 0 0 0 0 0 0 1 2 3 1 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 4 2 4 3 1 1 3 0 6 1 0 0 0 0 0 0 0 0 0
Cytoplasm 241 338 454 193 322 287 483 513 361 446 312 413 311 493 627 59 110 67 87 97 82 165 208 186
Endoplasmic Reticulum 6 8 5 2 2 4 3 2 0 0 3 8 3 7 2 5 10 4 8 12 17 8 9 21
Endosome 1 15 58 30 50 17 37 99 47 18 19 16 12 4 12 14 7 6 55 76 153 28 37 59
Golgi 0 3 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 4 8 24 3 3 7
Mitochondria 87 27 32 34 70 130 381 234 252 418 370 378 1 0 0 4 2 1 93 78 211 103 112 126
Nucleus 0 1 0 1 3 3 4 5 4 4 3 2 0 1 0 1 6 3 0 1 1 4 3 1
Nuclear Periphery 1 0 2 1 1 0 1 2 0 2 1 1 0 0 1 0 0 0 0 3 1 0 1 6
Nucleolus 0 0 1 0 0 0 4 2 2 2 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 7 1 2 0 3 8 0 1 1 1 1 0 6 0 0 0 3 0 2 1 0 0
SpindlePole 4 0 14 7 14 14 20 35 15 9 15 19 3 1 2 0 1 0 9 4 7 2 9 15
Vac/Vac Membrane 10 50 36 7 31 56 82 53 50 66 54 75 13 7 46 82 74 69 82 178 130 117 177 221
Unique Cell Count 307 410 571 265 446 433 859 832 633 798 639 748 336 506 656 133 165 129 355 477 663 451 583 686
Labelled Cell Count 355 446 621 288 516 531 1082 989 779 1030 843 980 351 522 698 166 210 150 355 477 663 451 583 686


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 5.1 4.6 4.6 4.8 8.0 4.3 4.3 4.4 4.2 4.0 4.1 6.0 5.7 5.4 6.8 6.7 6.9 5.9 6.2 6.4
Std Deviation (1e-4) 0.5 0.9 0.9 1.4 1.6 9.5 1.1 1.1 1.1 0.9 1.0 0.9 1.0 0.9 0.8 1.3 1.4 1.4 1.3 1.4 1.6
Intensity Change (Log2) 0.0 0.08 0.81 -0.11 -0.08 -0.06 -0.13 -0.19 -0.18 0.38 0.33 0.23 0.58 0.56 0.6 0.35 0.43 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.5 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.1 -2.7 -4.7 -9.1 -7.1 -8.3 -9.1 -11.1 -9.2 5.2 9.0 8.7 -8.2 -3.4 -6.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.8 0 0 0 0
Endosome 0 0 -3.7 0 0 0 0 0 0 -3.6 -6.6 -6.3 0.1 -2.4 -2.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 10.4 0 0 0 0 0 0 -4.1 -5.4 -6.1 -1.2 -2.4 -2.3
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 -0.5 0 0 0
SpindlePole 0 0 0.7 0 0 0 0 0 0 -1.7 -3.2 -3.3 0 0 0
Vacuole -2.2 0.4 3.6 2.2 0 1.1 1.4 1.4 2.4 -1.6 -4.1 0.5 15.4 12.2 13.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0585 1.9796 1.7831 1.4214 1.7918 1.8847 0.8385 1.1162 0.9983 0.8511 0.686 1.1715 -0.1206 0.6182 0.1066 0.431 0.1971 0.342
Actin 0.024 0.0002 0.0053 0.0005 0.015 0.008 0.0194 0.0028 0.0035 0.0018 0.0033 0.0053 0.0051 0.0009 0.0012 0 0.0121 0.002
Bud 0.0006 0.0002 0.0006 0.0005 0.0008 0.0031 0.0003 0.0028 0.001 0.0051 0.0005 0.0017 0.0007 0.0019 0.0033 0.0005 0.0007 0.003
Bud Neck 0.0056 0.0001 0.0009 0.0003 0.0005 0.0013 0.0015 0.001 0.0045 0.0004 0.0007 0.001 0.0022 0.0004 0.0005 0.0003 0.0019 0.0016
Bud Periphery 0.0009 0.0002 0.0007 0.0006 0.0019 0.0035 0.0003 0.0033 0.001 0.0107 0.0004 0.004 0.0008 0.0014 0.0023 0.0003 0.0005 0.0043
Bud Site 0.0042 0.0007 0.0015 0.0004 0.001 0.0003 0.0015 0.0154 0.0101 0.0022 0.0004 0.001 0.0048 0.0069 0.008 0.0002 0.0008 0.0008
Cell Periphery 0.0007 0.0002 0.0004 0.0002 0.0003 0.0002 0.0003 0.0017 0.0002 0.0001 0.0001 0.0001 0.0003 0.0004 0.0002 0 0.0002 0.0001
Cytoplasm 0.1675 0.2957 0.2377 0.2875 0.2713 0.3137 0.2457 0.3359 0.3453 0.2669 0.3246 0.4367 0.3191 0.4005 0.3553 0.3774 0.2929 0.4181
Cytoplasmic Foci 0.0993 0.0556 0.0737 0.077 0.0809 0.0842 0.0937 0.0868 0.0711 0.0593 0.0749 0.0844 0.0941 0.0904 0.0935 0.1162 0.0763 0.1063
Eisosomes 0.0005 0.0001 0.0009 0.0001 0.0014 0.0001 0.0005 0.0001 0 0 0 0.0001 0.0002 0.0001 0.0001 0 0.0013 0
Endoplasmic Reticulum 0.0066 0.0029 0.0124 0.0035 0.0061 0.0094 0.0072 0.0055 0.0063 0.0045 0.0032 0.0051 0.0084 0.0038 0.0043 0.002 0.0048 0.0039
Endosome 0.1704 0.1036 0.1572 0.1902 0.3135 0.1444 0.1674 0.1702 0.1421 0.2956 0.3455 0.1788 0.2177 0.1732 0.2239 0.3329 0.2333 0.2106
Golgi 0.0157 0.0022 0.0057 0.0054 0.0137 0.0046 0.0078 0.0054 0.0064 0.0047 0.0107 0.0038 0.0057 0.006 0.0104 0.0086 0.0087 0.0048
Lipid Particles 0.0189 0.0024 0.0097 0.0033 0.0202 0.0036 0.0072 0.0023 0.0026 0.0014 0.011 0.0026 0.0056 0.002 0.0024 0.0017 0.0053 0.002
Mitochondria 0.0164 0.0011 0.0081 0.0064 0.0327 0.0028 0.0028 0.0036 0.0037 0.0061 0.003 0.0038 0.0045 0.0049 0.0031 0.0029 0.0037 0.004
None 0.3738 0.4997 0.4347 0.3714 0.1649 0.3738 0.3882 0.3197 0.359 0.1989 0.13 0.2309 0.2833 0.2715 0.2553 0.0947 0.2142 0.2086
Nuclear Periphery 0.0039 0.0014 0.0037 0.0024 0.0111 0.0058 0.0089 0.0037 0.004 0.0307 0.0065 0.006 0.0057 0.0013 0.0016 0.0009 0.0098 0.0016
Nucleolus 0.0033 0.0003 0.0009 0.0005 0.0016 0.0003 0.0007 0.0005 0.0005 0.0009 0.0005 0.0003 0.0005 0.0005 0.0004 0.0002 0.0046 0.0002
Nucleus 0.004 0.0012 0.0021 0.002 0.0036 0.0031 0.0056 0.003 0.0023 0.009 0.0031 0.0032 0.0026 0.0021 0.0019 0.0021 0.0736 0.0015
Peroxisomes 0.0096 0.0005 0.0046 0.0012 0.0026 0.0042 0.0074 0.0019 0.0015 0.0016 0.0032 0.0009 0.0023 0.0009 0.0013 0.0007 0.0021 0.0019
Punctate Nuclear 0.0242 0.0008 0.0023 0.0009 0.0047 0.0051 0.0066 0.0021 0.0018 0.0052 0.0117 0.0017 0.0018 0.0013 0.0015 0.0004 0.0158 0.0012
Vacuole 0.0447 0.0271 0.0291 0.0359 0.0376 0.023 0.0231 0.0274 0.0266 0.0692 0.0508 0.0228 0.028 0.0247 0.0245 0.0486 0.0297 0.0175
Vacuole Periphery 0.0055 0.0038 0.0077 0.0098 0.0146 0.0054 0.0038 0.0048 0.0064 0.0255 0.016 0.0058 0.0065 0.0049 0.0053 0.0092 0.0078 0.0058

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.4527 4.3262 6.4205 6.9928 6.2746 2.4184 6.9259 8.3649 9.3749 8.362
Translational Efficiency 0.5075 0.8929 0.5129 0.5587 0.5105 1.9765 0.6487 0.5225 0.6148 0.7096

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2464 792 2253 1610 1973 2361 1249 99 4437 3153 3502 1709

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 724.36 708.15 817.49 794.84 685.60 735.68 852.45 885.19 707.12 728.76 829.96 800.07
Standard Deviation 83.42 117.74 105.66 107.47 81.48 88.17 109.43 147.05 84.78 97.19 108.32 112.15
Intensity Change Log 2 -0.032652 0.174494 0.133958 0.101711 0.314248 0.368620 0.034247 0.244142 0.252833

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000263 0.000678 0.000435 0.000785 0.000257 0.000487 0.000218 0.000178 0.000261 0.000535 0.000358 0.000750
Bud Neck 0.016245 0.018923 0.016840 0.013391 0.014201 0.019195 0.010453 0.002482 0.015336 0.019127 0.014562 0.012759
Bud Site 0.007240 0.018675 0.022653 0.047276 0.006366 0.018823 0.014109 0.038216 0.006852 0.018786 0.019606 0.046751
Cell Periphery 0.000081 0.000126 0.000074 0.000195 0.000079 0.000071 0.000077 0.000135 0.000080 0.000085 0.000075 0.000191
Cytoplasm 0.252415* 0.230476* 0.441693* 0.452453* 0.304653* 0.225898* 0.371664* 0.385808* 0.275643* 0.227048* 0.416717* 0.448592*
Cytoplasmic Foci 0.376202* 0.365068* 0.093141 0.034409 0.404938* 0.368387* 0.050262 0.039919 0.388980* 0.367553* 0.077848 0.034728
Eisosomes 0.000154 0.000122 0.000029 0.000048 0.000169 0.000105 0.000040 0.000057 0.000161 0.000109 0.000033 0.000049
Endoplasmic Reticulum 0.003310 0.002254 0.003219 0.002088 0.003183 0.001389 0.003252 0.001603 0.003254 0.001606 0.003231 0.002060
Endosome 0.072918 0.122078 0.033056 0.011651 0.054600 0.094360 0.034110 0.012161 0.064773 0.101322 0.033432 0.011681
Golgi 0.021453 0.021159 0.001946 0.004604 0.017531 0.030143 0.000400 0.023959 0.019709 0.027887 0.001395 0.005725
Lipid Particles 0.024238 0.016889 0.004829 0.001753 0.021149 0.011763 0.003969 0.001694 0.022865 0.013051 0.004522 0.001750
Mitochondria 0.014306 0.011921 0.003452 0.010833 0.011120 0.023019 0.002107 0.006637 0.012889 0.020231 0.002972 0.010590
Mitotic Spindle 0.003601 0.005417 0.032186 0.051723 0.002117 0.017414 0.006859 0.005475 0.002941 0.014400 0.023153 0.049044
None 0.001028 0.002755 0.001905 0.004596 0.000692 0.000593 0.004513 0.002040 0.000879 0.001136 0.002835 0.004448
Nuclear Periphery 0.000815 0.000438 0.005244 0.002204 0.000452 0.000375 0.003735 0.001227 0.000654 0.000391 0.004705 0.002148
Nuclear Periphery Foci 0.000400 0.001163 0.003276 0.001251 0.000435 0.000488 0.002930 0.002129 0.000416 0.000657 0.003152 0.001302
Nucleolus 0.000980 0.002533 0.002242 0.000801 0.000629 0.000833 0.003934 0.000613 0.000824 0.001260 0.002845 0.000790
Nucleus 0.041786 0.024464 0.140621* 0.162427* 0.045732 0.019315 0.220195* 0.094051 0.043541 0.020609 0.169001* 0.158466*
Peroxisomes 0.010547 0.020762 0.004774 0.001420 0.008941 0.016137 0.003865 0.000601 0.009833 0.017299 0.004450 0.001373
Vacuole 0.145125 0.125374 0.185451 0.191812 0.098597 0.141526 0.260787* 0.355029* 0.124435 0.137469 0.212320* 0.201267*
Vacuole Periphery 0.006892 0.008723 0.002933 0.004280 0.004160 0.009678 0.002522 0.025985 0.005677 0.009438 0.002787 0.005538

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.82 -2.97 -1.89 1.17 -0.07 -2.75 0.56 -0.03 2.53 -0.45 -3.62 -2.84 -2.04 0.59 -0.78
Bud Neck -1.51 -1.29 5.88 5.22 6.27 -4.24 2.18 9.82 13.60 5.79 -4.04 -0.20 6.29 8.89 5.70
Bud Site -5.62 -6.38 -6.90 -1.90 -1.73 -8.95 -2.83 -0.90 0.42 -0.32 -10.29 -7.05 -7.20 -2.00 -2.73
Cell Periphery -3.60 1.55 -3.25 0.62 -4.17 1.85 1.07 -1.84 -2.45 -2.20 -0.98 1.92 -3.61 -3.00 -4.37
Cytoplasm 2.08 -21.61 -16.53 -14.28 3.21 10.16 -6.09 0.07 -2.71 2.10 8.29 -20.47 -14.49 -19.93 1.69
Cytoplasmic Foci 1.37 54.12 73.89* 41.44 20.66 5.19 64.09 48.80 45.13 1.52 4.10 80.15 99.68 78.63 19.88
Eisosomes 3.50 18.64 16.00 11.22 -7.87 8.39 18.37 10.07 3.95 -5.24 9.12 26.33 22.62 16.85 -7.45
Endoplasmic Reticulum 2.22 -0.88 3.81 -0.08 6.20 4.37 -0.54 1.55 -3.36 3.01 6.37 -1.04 3.91 -4.32 7.54
Endosome -8.56 17.95 32.50* 21.78 14.11 -12.75 9.42 19.70 31.93 8.13 -13.71 19.65 38.83* 41.72 16.53
Golgi 0.30 17.26 16.03 10.00 -0.37 -7.21 15.85 1.84 3.89 -1.05 -5.89 22.93 17.75 20.16 -1.42
Lipid Particles 3.90 19.65 23.47 9.57 12.82 8.01 17.06 18.67 15.58 5.25 10.64 25.78 30.28 18.87 14.49
Mitochondria 1.64 12.63 7.24 4.37 -1.39 -7.19 8.43 5.00 10.32 -0.12 -5.91 14.91 7.21 10.99 -1.81
Mitotic Spindle -1.50 -11.79 -8.00 -7.06 1.19 -8.41 -5.18 -1.41 0.91 -0.39 -8.24 -12.78 -8.37 -4.08 -0.91
None -1.72 -4.24 -4.38 -0.62 -2.94 1.12 -6.41 -3.02 -3.13 0.80 -0.97 -8.01 -5.05 -4.12 -1.42
Nuclear Periphery 2.81 -11.29 -11.69 -15.06 5.15 1.05 -11.67 -5.61 -5.82 4.63 3.29 -15.20 -14.14 -16.27 5.70
Nuclear Periphery Foci -4.69 -8.42 -8.07 0.15 6.17 -0.26 -6.73 -4.47 -4.00 1.30 -1.58 -10.72 -9.15 -3.26 7.42
Nucleolus -3.42 -7.53 2.72 3.98 8.76 -2.98 -7.42 0.17 0.89 6.32 -3.44 -10.74 1.53 4.07 11.03
Nucleus 5.44 -26.73 -30.16 -30.92 -8.25 13.67 -27.83 -5.44 -6.97 5.07 15.05 -37.87 -30.76 -35.25 -3.53
Peroxisomes -4.78 7.86 16.28 9.65 7.92 -6.84 6.55 16.18 17.22 3.48 -7.89 10.18 21.51 19.04 7.81
Vacuole 2.75 -10.55 -18.44 -17.89 -9.77 -9.43 -21.53 -11.18 -9.81 -5.73 -3.64 -22.52 -23.98 -21.23 -8.54
Vacuole Periphery -2.17 11.51 10.06 8.57 1.45 -10.68 8.01 -0.45 0.40 -0.80 -8.30 13.58 6.52 11.39 0.30