Standard name
Description Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.75 0.98 0.83 0.69 0.76 0.68 0.65 0.62 0.61 0.57 0.57 0.64 0.76 0.82 0.85 0.76 0.78 0.77 0.87 0.8 0.86 0.85 0.86 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.08 0.09 0.26 0.31 0.29 0.29 0.39 0.41 0.37 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0.05 0.12 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.28 0 0.25 0.33 0.21 0.21 0.21 0.27 0.26 0.21 0.18 0.15 0.28 0.24 0.23 0.33 0.35 0.28 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3 1 1 1
Bud 0 1 2 1 0 4 14 5 3 12 1 4 0 0 0 0 0 0 0 0 2 2 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2 3
Bud Site 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 6 2 2 3 0 3 0 3 2 2 2 2 0 0 0 0 0 0
Cytoplasm 109 191 312 216 199 313 353 280 266 381 245 271 303 436 505 216 226 210 161 163 351 324 330 394
Endoplasmic Reticulum 0 2 1 0 1 0 0 0 0 0 1 3 0 6 3 9 10 10 3 1 5 1 2 2
Endosome 3 0 12 10 7 2 9 3 5 6 1 0 17 5 2 9 7 2 0 1 2 2 4 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 2 0 0 0 1 2 4
Mitochondria 6 1 7 25 23 118 167 130 126 261 176 157 0 0 1 0 3 3 0 1 4 2 1 6
Nucleus 2 5 4 11 10 11 22 20 18 29 18 13 27 34 20 8 3 9 9 25 15 22 15 13
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 2 0 1 1
Nucleolus 1 0 2 0 0 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 3
Vac/Vac Membrane 41 5 95 103 56 96 113 120 114 142 79 64 111 128 137 95 103 77 4 6 8 11 13 14
Unique Cell Count 145 195 377 311 263 459 547 449 433 663 431 426 401 529 594 286 291 271 186 205 407 380 383 461
Labelled Cell Count 162 205 435 366 296 545 686 562 534 836 522 518 459 612 670 339 357 315 186 205 407 380 383 461


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.3 6.3 5.8 5.9 5.3 5.0 5.3 5.0 4.8 4.5 4.6 7.7 7.7 7.3 7.8 8.0 7.5 7.5 7.8 8.1
Std Deviation (1e-4) 0.9 1.0 1.4 1.3 1.7 1.6 1.4 1.6 1.2 1.0 1.1 1.1 1.0 1.1 0.9 1.2 1.7 1.2 1.3 1.2 1.2
Intensity Change (Log2) – – – -0.13 -0.1 -0.25 -0.32 -0.25 -0.34 -0.39 -0.49 -0.45 0.28 0.28 0.22 0.31 0.34 0.25 0.25 0.31 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.1 -2.2 -4.8 -6.1 -6.5 -6.7 -8.3 -7.9 -6.1 -2.5 -0.1 0.9 -2.3 -1.6 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 -0.4 -3.1 -1.5 -2.7 -2.0 0 0 0 0.8 -2.4 -3.6 0 -0.6 -2.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.8 4.1 9.6 11.0 10.4 10.4 0 0 0 0 0 0 0 0 0
Nucleus 2.2 2.3 1.4 2.7 2.9 2.7 2.9 2.7 2.0 4.0 4.0 2.3 1.7 0 2.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.3 -1.1 -1.5 -1.6 0.5 0.4 -1.4 -2.4 -3.6 0.8 -0.3 -0.8 2.3 2.9 0.9