Standard name
Description Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.07 0.05 0 0 0.05 0 0 0 0 0.06 0.07 0.14 0 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.33 0 0 0 0.09 0.13 0.09 0.37 0.3 0.34 0.36 0 0 0 0.11 0.09 0 0 0 0 0 0 0
Nucleus 0.97 0.93 0.87 0.9 0.92 0.89 0.89 0.9 0.79 0.84 0.84 0.84 0.94 0.93 0.84 0.89 0.82 0.88 0.89 0.88 0.77 0.92 0.83 0.75
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 0 0 0 0 0 0 0.14 0.12 0.12 0.13 0.12 0 0 0 0 0 0 0 0.06 0 0 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0
Bud 0 0 0 0 0 0 1 1 2 4 2 5 0 0 0 2 0 0 1 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 2 6 3 15 11 18 24 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 1 16 17 17 12 16 19 10 8 8 8 1 10 19 4 8 7 1 0 7 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 2 1 0 1 0 0 0 2 3 0 0 0 0 0 0 1
Endosome 0 0 6 2 2 2 3 1 1 0 1 0 0 0 1 1 0 0 1 1 3 0 1 2
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Mitochondria 4 60 4 5 3 25 49 36 98 90 125 181 0 3 0 14 9 0 0 1 5 0 2 1
Nucleus 196 169 203 210 291 258 343 348 209 256 308 424 15 138 117 111 80 57 184 168 187 36 102 116
Nuclear Periphery 0 0 1 1 1 4 2 1 1 4 3 1 0 0 1 1 2 0 0 0 0 0 0 0
Nucleolus 4 10 9 3 4 10 16 7 36 36 45 65 2 4 2 3 3 2 5 2 14 0 4 13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 12 0 5 3 6 6 4 4 5 4 0 0 0 0 0 1 1 1 4 0 0 1
Vac/Vac Membrane 2 2 4 1 6 3 4 3 2 2 3 1 0 1 2 3 7 1 7 10 8 1 2 9
Unique Cell Count 203 181 234 233 317 289 385 386 263 303 366 502 16 149 139 125 97 65 207 190 244 40 124 156
Labelled Cell Count 210 243 255 241 330 320 447 426 380 416 518 715 18 156 142 142 112 68 207 190 244 40 124 156


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 4.6 7.2 6.4 6.7 6.1 5.6 5.9 4.9 5.1 4.6 4.8 5.4 6.6 6.0 5.5 7.9 7.6 5.4 7.6 7.8
Std Deviation (1e-4) 1.3 0.8 2.1 1.8 1.8 2.9 1.4 1.7 1.6 1.6 0.9 0.9 0.8 1.3 1.5 1.1 4.3 2.0 0.5 1.9 1.9
Intensity Change (Log2) – – – -0.17 -0.11 -0.25 -0.38 -0.3 -0.57 -0.52 -0.64 -0.61 -0.43 -0.13 -0.27 -0.39 0.12 0.06 -0.42 0.06 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8186 1.8022 1.5453 1.4534 1.2877 1.6229 4.5003 4.579 4.5801 4.1532 4.3059 4.7485 1.9691 2.3373 1.8562 1.7766 2.234 1.9098
Actin 0.0178 0 0.0082 0 0.0037 0.0002 0.0041 0 0.0013 0 0 0 0.0008 0 0.0117 0.0017 0.0171 0.0113
Bud 0.0002 0 0.0004 0 0.0001 0 0.0003 0 0 0 0 0 0.0002 0 0.0003 0.0001 0.0007 0.0001
Bud Neck 0.0006 0.0001 0.0036 0.0001 0.0005 0.0016 0.0009 0.0001 0.0004 0.0001 0.0003 0.0005 0.0023 0.0001 0.0003 0.0001 0.0012 0.0011
Bud Periphery 0.0005 0 0.001 0 0.0004 0.0001 0.0004 0 0.0001 0 0 0 0.0005 0 0.0003 0.0001 0.0012 0.0004
Bud Site 0.0007 0 0.004 0 0.0002 0.0001 0.0021 0 0.0001 0 0.0001 0.0001 0.0007 0.0006 0.007 0.0005 0.0038 0.0002
Cell Periphery 0.0002 0 0.0003 0 0.0001 0 0.0002 0 0 0 0 0 0.0001 0 0.0001 0 0.0002 0.0001
Cytoplasm 0.0013 0.0005 0.0002 0 0.0003 0.0004 0.0036 0.0008 0.0002 0.0001 0.0001 0 0.0102 0.0016 0.0016 0.0003 0.0012 0.0004
Cytoplasmic Foci 0.0089 0 0.003 0 0.0061 0.0001 0.0083 0 0.0004 0 0 0.0005 0.0035 0.0001 0.0064 0.0089 0.0007 0.0064
Eisosomes 0.0001 0 0.0002 0 0.0003 0 0 0 0 0 0 0 0 0 0.0001 0.0001 0.0003 0
Endoplasmic Reticulum 0.0011 0 0.0004 0 0.0021 0.0003 0.0031 0.0002 0.0002 0 0 0 0.0015 0.0001 0.0003 0.0007 0.0026 0.0004
Endosome 0.021 0 0.0046 0 0.006 0.0005 0.0111 0.0001 0.0013 0 0.0001 0.0004 0.0068 0.0001 0.0028 0.0025 0.005 0.0046
Golgi 0.0068 0 0.0035 0 0.0077 0 0.0035 0 0.0004 0 0 0.0004 0.0005 0 0.0017 0.0012 0.0011 0.0015
Lipid Particles 0.0123 0 0.0056 0 0.0443 0.0001 0.0145 0 0.0012 0 0 0.0052 0.0019 0 0.0037 0.0196 0.0012 0.0046
Mitochondria 0.0271 0.0001 0.0034 0.0001 0.038 0.0003 0.0024 0 0.0005 0 0.0002 0.0011 0.0014 0.0002 0.0089 0.0042 0.0099 0.0041
None 0.0011 0 0.0002 0 0.0002 0.0002 0.0014 0.0001 0.0005 0 0.0007 0 0.0059 0.0069 0.0003 0.001 0.0012 0.0004
Nuclear Periphery 0.0063 0.0035 0.0035 0.0005 0.007 0.0062 0.0125 0.0047 0.0024 0.0005 0.0015 0.0004 0.0184 0.0006 0.0013 0.0012 0.0169 0.0046
Nucleolus 0.0273 0.0165 0.0174 0.0133 0.0614 0.0537 0.0166 0.0111 0.0107 0.0107 0.0421 0.0437 0.023 0.016 0.0165 0.0121 0.0304 0.0329
Nucleus 0.8558 0.9736 0.9336 0.9641 0.8065 0.9329 0.8832 0.9791 0.9783 0.9876 0.949 0.944 0.8911 0.9713 0.9239 0.9401 0.8956 0.9133
Peroxisomes 0.0033 0 0.0031 0 0.0025 0 0.0097 0 0.0003 0 0 0.0018 0.0003 0 0.0009 0.003 0.0007 0.0014
Punctate Nuclear 0.0056 0.0055 0.0019 0.0219 0.0097 0.0027 0.0179 0.0037 0.0013 0.0008 0.0055 0.0014 0.0278 0.0019 0.0112 0.0019 0.007 0.0112
Vacuole 0.0012 0 0.0012 0 0.0006 0.0005 0.0031 0 0.0002 0 0.0001 0.0001 0.002 0.0002 0.0004 0.0003 0.001 0.0004
Vacuole Periphery 0.0008 0 0.0007 0 0.0021 0.0002 0.0011 0 0.0002 0 0.0001 0.0001 0.001 0 0.0002 0.0004 0.0009 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.1248 5.0197 9.3121 9.1438 11.8308 15.4335 13.323 10.2767 10.0582 12.6132
Translational Efficiency 1.1562 2.9735 1.1492 1.2971 0.89 0.798 0.8803 1.266 1.489 0.7602

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘