Standard name
Description Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA; relocalizes to the cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.07 0.05 0 0 0.05 0 0 0 0 0.06 0.07 0.14 0 0.08 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.33 0 0 0 0.09 0.13 0.09 0.37 0.3 0.34 0.36 0 0 0 0.11 0.09 0 0 0 0 0 0 0
Nucleus 0.97 0.93 0.87 0.9 0.92 0.89 0.89 0.9 0.79 0.84 0.84 0.84 0.94 0.93 0.84 0.89 0.82 0.88 0.89 0.88 0.77 0.92 0.83 0.75
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 0 0 0 0 0 0 0.14 0.12 0.12 0.13 0.12 0 0 0 0 0 0 0 0.06 0 0 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 0
Bud 0 0 0 0 0 0 1 1 2 4 2 5 0 0 0 2 0 0 1 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 2 6 3 15 11 18 24 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 1 16 17 17 12 16 19 10 8 8 8 1 10 19 4 8 7 1 0 7 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 1 0 0 2 1 0 1 0 0 0 2 3 0 0 0 0 0 0 1
Endosome 0 0 6 2 2 2 3 1 1 0 1 0 0 0 1 1 0 0 1 1 3 0 1 2
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1
Mitochondria 4 60 4 5 3 25 49 36 98 90 125 181 0 3 0 14 9 0 0 1 5 0 2 1
Nucleus 196 169 203 210 291 258 343 348 209 256 308 424 15 138 117 111 80 57 184 168 187 36 102 116
Nuclear Periphery 0 0 1 1 1 4 2 1 1 4 3 1 0 0 1 1 2 0 0 0 0 0 0 0
Nucleolus 4 10 9 3 4 10 16 7 36 36 45 65 2 4 2 3 3 2 5 2 14 0 4 13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 12 0 5 3 6 6 4 4 5 4 0 0 0 0 0 1 1 1 4 0 0 1
Vac/Vac Membrane 2 2 4 1 6 3 4 3 2 2 3 1 0 1 2 3 7 1 7 10 8 1 2 9
Unique Cell Count 203 181 234 233 317 289 385 386 263 303 366 502 16 149 139 125 97 65 207 190 244 40 124 156
Labelled Cell Count 210 243 255 241 330 320 447 426 380 416 518 715 18 156 142 142 112 68 207 190 244 40 124 156


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 4.6 7.2 6.4 6.7 6.1 5.6 5.9 4.9 5.1 4.6 4.8 5.4 6.6 6.0 5.5 7.9 7.6 5.4 7.6 7.8
Std Deviation (1e-4) 1.3 0.8 2.1 1.8 1.8 2.9 1.4 1.7 1.6 1.6 0.9 0.9 0.8 1.3 1.5 1.1 4.3 2.0 0.5 1.9 1.9
Intensity Change (Log2) -0.17 -0.11 -0.25 -0.38 -0.3 -0.57 -0.52 -0.64 -0.61 -0.43 -0.13 -0.27 -0.39 0.12 0.06 -0.42 0.06 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Luc7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available