Standard name
Human Ortholog
Description Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.62 0.58 0.85 0.85 0.91 0.66 0.59 0.6 0.51 0.49 0.43 0.38 0.96 0.99 0.99 0.91 0.91 0.83 0.41 0.43 0.38 0.65 0.87 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0.24 0.07 0.24 0.19 0.51 0.63 0.55 0.65 0.74 0.77 0.75 0 0 0 0 0 0 0.14 0 0.26 0 0 0
Nucleus 0.05 0.15 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0.21 0.07 0.1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Vac/Vac Membrane 0.08 0.14 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.1 0.16 0.19 0.12 0.11 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 4 1 2 1
Bud 0 3 2 2 1 4 9 5 14 8 13 13 0 1 0 0 0 1 1 4 3 1 3 1
Bud Neck 2 0 1 0 0 4 5 0 1 2 0 0 0 0 3 0 0 1 1 0 1 0 2 1
Bud Site 0 0 0 0 0 0 3 2 3 3 2 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 1 1 1 1 1 0 0 0 1 0 0 2 0 0 0 1 0 0 1
Cytoplasm 124 76 164 169 156 180 230 187 179 194 134 134 164 320 348 60 122 85 91 68 92 129 295 308
Endoplasmic Reticulum 1 1 0 2 0 2 1 0 0 4 1 0 3 0 1 2 16 6 0 0 3 0 1 0
Endosome 0 0 5 2 3 1 0 1 2 0 0 1 0 0 1 0 2 4 3 0 4 1 2 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 1 3
Mitochondria 85 32 13 48 32 138 246 171 229 294 240 268 0 1 1 0 2 4 31 6 64 5 8 8
Nucleus 9 20 4 0 1 1 6 7 8 9 9 4 2 0 0 1 3 7 28 33 16 19 2 0
Nuclear Periphery 0 2 0 1 0 1 1 1 0 4 3 0 0 0 2 0 0 0 0 1 2 0 1 0
Nucleolus 0 0 1 1 0 2 1 3 0 0 0 0 0 1 0 0 0 0 0 1 1 2 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 2 1 1 3 1 5 5 1 5 0 1 0 0 0 0 15 5 7 7 5 4
Vac/Vac Membrane 16 18 15 6 3 11 11 7 7 15 11 9 3 1 0 4 9 10 36 29 28 22 6 4
Unique Cell Count 199 131 193 198 171 271 392 312 352 395 310 357 171 323 351 66 134 102 224 158 245 200 341 350
Labelled Cell Count 238 153 207 233 197 346 517 386 449 539 414 439 172 326 356 67 157 118 224 158 245 200 341 350


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 4.0 3.8 3.6 3.8 3.1 3.0 3.0 3.0 2.7 2.7 2.5 5.0 4.8 4.8 4.9 5.0 5.0 4.7 5.0 5.4
Std Deviation (1e-4) 0.5 1.1 1.0 1.6 1.9 1.4 1.7 1.3 1.4 0.9 1.3 0.9 1.7 1.2 1.3 1.3 1.4 1.5 0.9 1.3 1.8
Intensity Change (Log2) – – – -0.11 -0.02 -0.32 -0.34 -0.38 -0.38 -0.53 -0.53 -0.63 0.39 0.32 0.3 0.36 0.37 0.39 0.28 0.37 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – -0.4 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.47 1.3743 0.987 0.7005 0.7786 0.8723 -0.4247 -0.4586 -0.447 -0.5063 -0.0187 -0.3079 -1.0572 -1.5714 -1.4073 -1.1883 -0.968 -1.4591
Actin 0.0011 0.0001 0.007 0.0014 0.0025 0.0042 0.023 0.0025 0.0015 0.0007 0.0292 0.0014 0.0475 0.0003 0.019 0.02 0.007 0.0039
Bud 0.0001 0.0002 0.0004 0.0006 0.0001 0.0045 0.0009 0.0047 0.0015 0.0002 0.0002 0.002 0.0008 0.0006 0.0005 0.0003 0.0001 0.0002
Bud Neck 0.0201 0.0005 0.0004 0.0003 0.0009 0.0058 0.0018 0.0005 0.0004 0.0016 0.0009 0.0053 0.0138 0.0008 0.0006 0.0129 0.0007 0.0042
Bud Periphery 0.0002 0.0002 0.0011 0.001 0.0001 0.009 0.0017 0.0039 0.0019 0.0003 0.0002 0.0033 0.0017 0.0008 0.0007 0.0003 0.0001 0.0002
Bud Site 0.0007 0.0006 0.0006 0.0001 0.0002 0.0005 0.0047 0.0077 0.008 0.0017 0.004 0.0019 0.013 0.0138 0.0011 0.0039 0.0001 0.0004
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0005 0.0003 0.0001 0.0001 0.0001 0.0001 0.0006 0.0002 0.0001 0.0001 0 0
Cytoplasm 0.0647 0.077 0.0821 0.0814 0.0863 0.1094 0.1032 0.1748 0.1746 0.0829 0.0381 0.1209 0.1106 0.1916 0.122 0.1846 0.1242 0.1477
Cytoplasmic Foci 0.0049 0.003 0.0107 0.0027 0.0128 0.0186 0.0293 0.0117 0.0141 0.0158 0.0225 0.0084 0.0278 0.0051 0.0121 0.0106 0.0053 0.0111
Eisosomes 0.0005 0.0001 0.0005 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0 0.0001 0 0.0006 0.0001 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0012 0.0013 0.0024 0.0065 0.0016 0.0018 0.0075 0.0028 0.0024 0.0014 0.0011 0.0016 0.0039 0.0041 0.0021 0.0043 0.0061 0.0017
Endosome 0.0022 0.0017 0.0112 0.0048 0.0039 0.0031 0.0414 0.0035 0.015 0.0047 0.0344 0.0124 0.0215 0.0021 0.0074 0.0092 0.0112 0.0025
Golgi 0.0004 0 0.0026 0.0001 0.0004 0.0004 0.0101 0.0011 0.0019 0.0028 0.0076 0.0115 0.0062 0.0001 0.0037 0.0059 0.0017 0.0011
Lipid Particles 0.0013 0.0003 0.0046 0.0009 0.002 0.001 0.0207 0.003 0.0042 0.0174 0.0048 0.0065 0.0171 0.0009 0.015 0.0106 0.004 0.0011
Mitochondria 0.0006 0.0005 0.0017 0.0016 0.0007 0.002 0.0121 0.0022 0.0039 0.0008 0.0026 0.013 0.0162 0.0009 0.004 0.0037 0.0021 0.0089
None 0.6841 0.851 0.7068 0.4888 0.4534 0.4231 0.4672 0.643 0.5625 0.2851 0.1719 0.2433 0.4146 0.5766 0.5679 0.166 0.1976 0.2493
Nuclear Periphery 0.005 0.0019 0.0057 0.0494 0.0094 0.0047 0.0149 0.0033 0.0096 0.0161 0.005 0.0088 0.0167 0.0088 0.0163 0.0228 0.0562 0.0083
Nucleolus 0.0027 0.0017 0.0023 0.0023 0.0052 0.0066 0.0054 0.003 0.0018 0.0037 0.0063 0.0094 0.0081 0.002 0.0021 0.0021 0.0044 0.0064
Nucleus 0.1873 0.0502 0.146 0.3404 0.3596 0.3628 0.217 0.1138 0.1672 0.5182 0.5833 0.5117 0.2407 0.1624 0.1893 0.5111 0.5343 0.5147
Peroxisomes 0.0006 0.0001 0.002 0.0002 0.0008 0.0046 0.0126 0.0065 0.0061 0.0071 0.0177 0.0041 0.016 0.001 0.0045 0.0145 0.0019 0.0012
Punctate Nuclear 0.0209 0.0082 0.0091 0.0132 0.0589 0.0359 0.0147 0.0082 0.0186 0.0376 0.0685 0.0315 0.0177 0.0254 0.0295 0.0148 0.0394 0.0355
Vacuole 0.0011 0.0011 0.0021 0.0027 0.0008 0.0015 0.0077 0.0029 0.0033 0.0012 0.0012 0.0022 0.0035 0.002 0.0012 0.0012 0.0015 0.0011
Vacuole Periphery 0.0002 0.0001 0.0006 0.0014 0.0002 0.0003 0.0031 0.0004 0.0013 0.0008 0.0003 0.0007 0.0014 0.0003 0.0007 0.0005 0.0018 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 364.0988 407.4962 430.0997 467.0105 392.0581 302.6309 433.5868 424.406 382.5467 414.6182
Translational Efficiency 1.6498 1.7831 1.6595 1.3804 1.7202 2.1997 1.5968 1.5896 1.6242 1.5689

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
97 393 2029 1577 953 1971 1616 205 1050 2364 3645 1782

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 711.20 737.91 797.01 827.81 651.78 717.81 851.39 900.40 657.27 721.15 821.12 836.16
Standard Deviation 141.56 98.19 92.95 111.07 71.22 80.57 102.01 100.31 82.16 84.09 100.76 112.30
Intensity Change Log 2 — 0.053190 0.164343 0.219044 — 0.139217 0.385435 0.466181 — 0.094968 0.274302 0.342514

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000545 0.000937 0.000139 0.000747 0.000120 0.000307 0.000172 0.000778 0.000159 0.000411 0.000154 0.000750
Bud Neck 0.066044 0.026982 0.007164 0.011699 0.005430 0.023449 0.005589 0.003975 0.011029 0.024036 0.006465 0.010810
Bud Site 0.048379 0.011854 0.003727 0.017150 0.006233 0.008915 0.003491 0.013213 0.010127 0.009404 0.003622 0.016697
Cell Periphery 0.000834 0.000191 0.000210 0.000165 0.000482 0.000222 0.000122 0.000142 0.000515 0.000217 0.000171 0.000163
Cytoplasm 0.189077* 0.309364* 0.082413 0.133603* 0.386935* 0.331936* 0.084218 0.207418* 0.368657* 0.328183* 0.083213 0.142094*
Cytoplasmic Foci 0.076543 0.164684 0.003531 0.005164 0.101299 0.128937 0.002929 0.052638 0.099012 0.134880 0.003264 0.010625
Eisosomes 0.000216 0.000130 0.000048 0.000038 0.000099 0.000103 0.000029 0.000071 0.000110 0.000107 0.000040 0.000042
Endoplasmic Reticulum 0.026236 0.001444 0.001838 0.006427 0.000528 0.000856 0.002389 0.004497 0.002903 0.000953 0.002082 0.006205
Endosome 0.045529 0.015513 0.000769 0.004916 0.003102 0.008560 0.001001 0.035482 0.007021 0.009716 0.000872 0.008433
Golgi 0.004260 0.002759 0.000041 0.004338 0.000456 0.002316 0.000316 0.026738 0.000808 0.002390 0.000163 0.006915
Lipid Particles 0.013310 0.008463 0.000541 0.001566 0.007995 0.008840 0.001117 0.002111 0.008486 0.008777 0.000796 0.001628
Mitochondria 0.020710 0.012682 0.000829 0.004908 0.002782 0.013569 0.001156 0.004860 0.004438 0.013422 0.000974 0.004902
Mitotic Spindle 0.022523 0.007537 0.000941 0.036804 0.000145 0.010853 0.004065 0.030458 0.002213 0.010302 0.002326 0.036074
None 0.008084 0.008296 0.007485 0.007410 0.008656 0.005141 0.007298 0.005947 0.008603 0.005665 0.007402 0.007242
Nuclear Periphery 0.003361 0.000951 0.001083 0.004602 0.000825 0.001244 0.001413 0.001961 0.001059 0.001195 0.001229 0.004298
Nuclear Periphery Foci 0.008685 0.000709 0.000157 0.002112 0.000285 0.000338 0.000201 0.001314 0.001061 0.000400 0.000177 0.002020
Nucleolus 0.009465 0.004910 0.001454 0.002189 0.001870 0.003229 0.001400 0.000986 0.002571 0.003508 0.001430 0.002050
Nucleus 0.323145* 0.357475* 0.880336* 0.707113* 0.457286* 0.406205* 0.869585* 0.419506* 0.444894* 0.398104* 0.875569* 0.674027*
Peroxisomes 0.009361 0.017837 0.000131 0.001255 0.002576 0.008981 0.000361 0.006533 0.003203 0.010454 0.000233 0.001862
Vacuole 0.103842 0.045984 0.006970 0.043190 0.012320 0.034602 0.012559 0.169041 0.020775 0.036494 0.009448 0.057668
Vacuole Periphery 0.019851 0.001300 0.000194 0.004606 0.000576 0.001398 0.000589 0.012331 0.002356 0.001381 0.000369 0.005495

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.96 2.48 -0.65 0.70 -7.31 -2.27 -2.65 -4.59 -2.61 -3.77 -2.77 -0.96 -7.22 -2.88 -7.68
Bud Neck 2.49 3.84 3.50 3.59 -4.06 -9.53 -1.19 -0.55 9.31 0.72 -5.89 2.01 -0.74 6.72 -4.73
Bud Site 2.53 3.13 2.49 -0.26 -5.01 -1.41 1.88 -1.79 -1.17 -2.77 0.38 3.40 -1.04 -1.88 -5.80
Cell Periphery 4.57 4.57 5.03 1.77 3.24 2.77 3.89 3.72 3.86 -1.13 3.42 4.06 4.34 4.15 1.95
Cytoplasm -4.37 4.93 2.80 12.82 -8.41 5.47 32.91 13.52 10.93 -4.87 4.36 34.06 25.88 28.22 -9.67
Cytoplasmic Foci -6.83 8.08 7.98 17.23 -1.74 -4.61 21.84 13.00 18.57 -4.22 -6.52 22.99 22.07 34.68 -4.54
Eisosomes 2.83 5.97 5.95 6.18 -1.10 -0.52 12.29 -1.66 -1.30 -8.50 0.29 8.83 8.00 8.19 -3.70
Endoplasmic Reticulum 2.13 2.05 1.48 -19.16 -17.80 -3.88 -17.25 -9.75 -9.26 -5.11 1.75 -0.14 -5.31 -25.09 -17.77
Endosome 3.81 6.19 5.83 4.41 -7.20 -7.48 4.86 -2.16 -0.61 -2.76 -2.64 7.23 2.84 6.54 -6.35
Golgi 1.20 3.87 2.00 1.02 -2.51 -4.31 1.39 -1.30 -0.82 -1.36 -3.99 4.53 -1.77 0.31 -2.73
Lipid Particles 1.57 4.84 4.38 4.08 -6.11 -0.70 9.25 6.89 6.98 -3.91 -0.21 11.02 9.44 9.19 -5.20
Mitochondria 1.29 3.42 3.00 2.87 -5.22 -6.13 1.42 0.77 7.37 -0.58 -5.27 2.95 1.14 7.89 -4.88
Mitotic Spindle 1.41 2.05 -0.33 -5.13 -8.12 -6.17 -3.76 -2.66 -1.42 -2.13 -4.67 -0.57 -7.77 -4.61 -7.81
None -0.01 0.41 1.24 1.36 1.85 3.29 1.96 2.66 0.16 1.00 2.92 1.86 3.05 0.52 1.82
Nuclear Periphery 2.10 1.79 -2.78 -14.57 -13.81 -3.73 -12.15 -8.14 -6.56 -4.12 -1.09 -4.86 -14.57 -14.45 -13.42
Nuclear Periphery Foci 2.00 2.15 1.48 -4.06 -5.31 -0.69 -1.00 -3.10 -3.05 -3.01 1.73 2.08 -3.14 -5.53 -5.82
Nucleolus 1.53 3.05 2.45 2.26 -2.50 -4.22 0.35 1.40 5.58 0.83 -2.61 2.49 -0.33 1.65 -2.23
Nucleus -0.71 -16.34 -10.31 -19.41 22.16 4.34 -36.54 1.22 -0.76 17.32 4.28 -43.17 -17.25 -25.69 25.68
Peroxisomes -2.94 4.83 4.46 7.18 -1.94 -6.62 3.06 0.64 7.21 -2.10 -7.65 4.31 3.12 13.08 -2.28
Vacuole 3.46 6.47 0.62 -6.91 -19.40 -10.88 -5.61 -13.36 -12.30 -12.77 -6.65 2.32 -19.03 -15.97 -20.90
Vacuole Periphery 3.22 3.41 3.10 -0.98 -2.87 -3.02 0.53 -0.95 -0.58 -1.01 1.60 3.54 0.47 -0.96 -2.84
Standard name
Human Ortholog
Description Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress
Localization
Cell Percentages nucleus (33%), cytoplasm (9%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sub2

Sub2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sub2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available