Standard name
Description Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.62 0.58 0.85 0.85 0.91 0.66 0.59 0.60 0.51 0.49 0.43 0.38 0.96 0.99 0.99 0.91 0.91 0.83 0.41 0.43 0.38 0.65 0.87 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.43 0.24 0.07 0.24 0.19 0.51 0.63 0.55 0.65 0.74 0.77 0.75 0 0 0 0 0 0 0.14 0 0.26 0 0 0
Nucleus 0.05 0.15 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.13 0.21 0.07 0.10 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Vac/Vac Membrane 0.08 0.14 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.10 0.16 0.19 0.12 0.11 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 4 1 2 1
Bud 0 3 2 2 1 4 9 5 14 8 13 13 0 1 0 0 0 1 1 4 3 1 3 1
Bud Neck 2 0 1 0 0 4 5 0 1 2 0 0 0 0 3 0 0 1 1 0 1 0 2 1
Bud Site 0 0 0 0 0 0 3 2 3 3 2 5 0 0 0 0 0 0
Cell Periphery 0 0 1 0 0 1 1 1 1 1 0 0 0 1 0 0 2 0 0 0 1 0 0 1
Cytoplasm 124 76 164 169 156 180 230 187 179 194 134 134 164 320 348 60 122 85 91 68 92 129 295 308
Endoplasmic Reticulum 1 1 0 2 0 2 1 0 0 4 1 0 3 0 1 2 16 6 0 0 3 0 1 0
Endosome 0 0 5 2 3 1 0 1 2 0 0 1 0 0 1 0 2 4 3 0 4 1 2 6
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 1 3
Mitochondria 85 32 13 48 32 138 246 171 229 294 240 268 0 1 1 0 2 4 31 6 64 5 8 8
Nucleus 9 20 4 0 1 1 6 7 8 9 9 4 2 0 0 1 3 7 28 33 16 19 2 0
Nuclear Periphery 0 2 0 1 0 1 1 1 0 4 3 0 0 0 2 0 0 0 0 1 2 0 1 0
Nucleolus 0 0 1 1 0 2 1 3 0 0 0 0 0 1 0 0 0 0 0 1 1 2 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 2 1 1 3 1 5 5 1 5 0 1 0 0 0 0 15 5 7 7 5 4
Vac/Vac Membrane 16 18 15 6 3 11 11 7 7 15 11 9 3 1 0 4 9 10 36 29 28 22 6 4
Unique Cell Count 199 131 193 198 171 271 392 312 352 395 310 357 171 323 351 66 134 102 224 158 245 200 341 350
Labelled Cell Count 238 153 207 233 197 346 517 386 449 539 414 439 172 326 356 67 157 118 224 158 245 200 341 350

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 4.0 3.8 3.6 3.8 3.1 3.0 3.0 3.0 2.7 2.7 2.5 5.0 4.8 4.8 4.9 5.0 5.0 4.7 5.0 5.4
Std Deviation (1e-4) 0.5 1.1 1.0 1.6 1.9 1.4 1.7 1.3 1.4 0.9 1.3 0.9 1.7 1.2 1.3 1.3 1.4 1.5 0.9 1.3 1.8
Intensity Change (Log2) -0.1 -0.0 -0.3 -0.3 -0.4 -0.4 -0.5 -0.5 -0.6 0.4 0.3 0.3 0.4 0.4 0.4 0.3 0.4 0.5

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 0 0