Standard name | |
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Human Ortholog | |
Description | Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 1 | 2 | 1 |
Bud | 0 | 3 | 2 | 2 | 1 | 4 | 9 | 5 | 14 | 8 | 13 | 13 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 3 | 1 | 3 | 1 |
Bud Neck | 2 | 0 | 1 | 0 | 0 | 4 | 5 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 3 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 124 | 76 | 164 | 169 | 156 | 180 | 230 | 187 | 179 | 194 | 134 | 134 | 164 | 320 | 348 | 60 | 122 | 85 | 91 | 68 | 92 | 129 | 295 | 308 |
Endoplasmic Reticulum | 1 | 1 | 0 | 2 | 0 | 2 | 1 | 0 | 0 | 4 | 1 | 0 | 3 | 0 | 1 | 2 | 16 | 6 | 0 | 0 | 3 | 0 | 1 | 0 |
Endosome | 0 | 0 | 5 | 2 | 3 | 1 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 4 | 3 | 0 | 4 | 1 | 2 | 6 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 3 |
Mitochondria | 85 | 32 | 13 | 48 | 32 | 138 | 246 | 171 | 229 | 294 | 240 | 268 | 0 | 1 | 1 | 0 | 2 | 4 | 31 | 6 | 64 | 5 | 8 | 8 |
Nucleus | 9 | 20 | 4 | 0 | 1 | 1 | 6 | 7 | 8 | 9 | 9 | 4 | 2 | 0 | 0 | 1 | 3 | 7 | 28 | 33 | 16 | 19 | 2 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 4 | 3 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 2 | 1 | 1 | 3 | 1 | 5 | 5 | 1 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 15 | 5 | 7 | 7 | 5 | 4 |
Vac/Vac Membrane | 16 | 18 | 15 | 6 | 3 | 11 | 11 | 7 | 7 | 15 | 11 | 9 | 3 | 1 | 0 | 4 | 9 | 10 | 36 | 29 | 28 | 22 | 6 | 4 |
Unique Cell Count | 199 | 131 | 193 | 198 | 171 | 271 | 392 | 312 | 352 | 395 | 310 | 357 | 171 | 323 | 351 | 66 | 134 | 102 | 224 | 158 | 245 | 200 | 341 | 350 |
Labelled Cell Count | 238 | 153 | 207 | 233 | 197 | 346 | 517 | 386 | 449 | 539 | 414 | 439 | 172 | 326 | 356 | 67 | 157 | 118 | 224 | 158 | 245 | 200 | 341 | 350 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.0 | 4.0 | 3.8 | 3.6 | 3.8 | 3.1 | 3.0 | 3.0 | 3.0 | 2.7 | 2.7 | 2.5 | 5.0 | 4.8 | 4.8 | 4.9 | 5.0 | 5.0 | 4.7 | 5.0 | 5.4 |
Std Deviation (1e-4) | 0.5 | 1.1 | 1.0 | 1.6 | 1.9 | 1.4 | 1.7 | 1.3 | 1.4 | 0.9 | 1.3 | 0.9 | 1.7 | 1.2 | 1.3 | 1.3 | 1.4 | 1.5 | 0.9 | 1.3 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.02 | -0.32 | -0.34 | -0.38 | -0.38 | -0.53 | -0.53 | -0.63 | 0.39 | 0.32 | 0.3 | 0.36 | 0.37 | 0.39 | 0.28 | 0.37 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | -0.4 | 0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.47 | 1.3743 | 0.987 | 0.7005 | 0.7786 | 0.8723 | -0.4247 | -0.4586 | -0.447 | -0.5063 | -0.0187 | -0.3079 | -1.0572 | -1.5714 | -1.4073 | -1.1883 | -0.968 | -1.4591 |
Actin | 0.0011 | 0.0001 | 0.007 | 0.0014 | 0.0025 | 0.0042 | 0.023 | 0.0025 | 0.0015 | 0.0007 | 0.0292 | 0.0014 | 0.0475 | 0.0003 | 0.019 | 0.02 | 0.007 | 0.0039 |
Bud | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0.0045 | 0.0009 | 0.0047 | 0.0015 | 0.0002 | 0.0002 | 0.002 | 0.0008 | 0.0006 | 0.0005 | 0.0003 | 0.0001 | 0.0002 |
Bud Neck | 0.0201 | 0.0005 | 0.0004 | 0.0003 | 0.0009 | 0.0058 | 0.0018 | 0.0005 | 0.0004 | 0.0016 | 0.0009 | 0.0053 | 0.0138 | 0.0008 | 0.0006 | 0.0129 | 0.0007 | 0.0042 |
Bud Periphery | 0.0002 | 0.0002 | 0.0011 | 0.001 | 0.0001 | 0.009 | 0.0017 | 0.0039 | 0.0019 | 0.0003 | 0.0002 | 0.0033 | 0.0017 | 0.0008 | 0.0007 | 0.0003 | 0.0001 | 0.0002 |
Bud Site | 0.0007 | 0.0006 | 0.0006 | 0.0001 | 0.0002 | 0.0005 | 0.0047 | 0.0077 | 0.008 | 0.0017 | 0.004 | 0.0019 | 0.013 | 0.0138 | 0.0011 | 0.0039 | 0.0001 | 0.0004 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0647 | 0.077 | 0.0821 | 0.0814 | 0.0863 | 0.1094 | 0.1032 | 0.1748 | 0.1746 | 0.0829 | 0.0381 | 0.1209 | 0.1106 | 0.1916 | 0.122 | 0.1846 | 0.1242 | 0.1477 |
Cytoplasmic Foci | 0.0049 | 0.003 | 0.0107 | 0.0027 | 0.0128 | 0.0186 | 0.0293 | 0.0117 | 0.0141 | 0.0158 | 0.0225 | 0.0084 | 0.0278 | 0.0051 | 0.0121 | 0.0106 | 0.0053 | 0.0111 |
Eisosomes | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0012 | 0.0013 | 0.0024 | 0.0065 | 0.0016 | 0.0018 | 0.0075 | 0.0028 | 0.0024 | 0.0014 | 0.0011 | 0.0016 | 0.0039 | 0.0041 | 0.0021 | 0.0043 | 0.0061 | 0.0017 |
Endosome | 0.0022 | 0.0017 | 0.0112 | 0.0048 | 0.0039 | 0.0031 | 0.0414 | 0.0035 | 0.015 | 0.0047 | 0.0344 | 0.0124 | 0.0215 | 0.0021 | 0.0074 | 0.0092 | 0.0112 | 0.0025 |
Golgi | 0.0004 | 0 | 0.0026 | 0.0001 | 0.0004 | 0.0004 | 0.0101 | 0.0011 | 0.0019 | 0.0028 | 0.0076 | 0.0115 | 0.0062 | 0.0001 | 0.0037 | 0.0059 | 0.0017 | 0.0011 |
Lipid Particles | 0.0013 | 0.0003 | 0.0046 | 0.0009 | 0.002 | 0.001 | 0.0207 | 0.003 | 0.0042 | 0.0174 | 0.0048 | 0.0065 | 0.0171 | 0.0009 | 0.015 | 0.0106 | 0.004 | 0.0011 |
Mitochondria | 0.0006 | 0.0005 | 0.0017 | 0.0016 | 0.0007 | 0.002 | 0.0121 | 0.0022 | 0.0039 | 0.0008 | 0.0026 | 0.013 | 0.0162 | 0.0009 | 0.004 | 0.0037 | 0.0021 | 0.0089 |
None | 0.6841 | 0.851 | 0.7068 | 0.4888 | 0.4534 | 0.4231 | 0.4672 | 0.643 | 0.5625 | 0.2851 | 0.1719 | 0.2433 | 0.4146 | 0.5766 | 0.5679 | 0.166 | 0.1976 | 0.2493 |
Nuclear Periphery | 0.005 | 0.0019 | 0.0057 | 0.0494 | 0.0094 | 0.0047 | 0.0149 | 0.0033 | 0.0096 | 0.0161 | 0.005 | 0.0088 | 0.0167 | 0.0088 | 0.0163 | 0.0228 | 0.0562 | 0.0083 |
Nucleolus | 0.0027 | 0.0017 | 0.0023 | 0.0023 | 0.0052 | 0.0066 | 0.0054 | 0.003 | 0.0018 | 0.0037 | 0.0063 | 0.0094 | 0.0081 | 0.002 | 0.0021 | 0.0021 | 0.0044 | 0.0064 |
Nucleus | 0.1873 | 0.0502 | 0.146 | 0.3404 | 0.3596 | 0.3628 | 0.217 | 0.1138 | 0.1672 | 0.5182 | 0.5833 | 0.5117 | 0.2407 | 0.1624 | 0.1893 | 0.5111 | 0.5343 | 0.5147 |
Peroxisomes | 0.0006 | 0.0001 | 0.002 | 0.0002 | 0.0008 | 0.0046 | 0.0126 | 0.0065 | 0.0061 | 0.0071 | 0.0177 | 0.0041 | 0.016 | 0.001 | 0.0045 | 0.0145 | 0.0019 | 0.0012 |
Punctate Nuclear | 0.0209 | 0.0082 | 0.0091 | 0.0132 | 0.0589 | 0.0359 | 0.0147 | 0.0082 | 0.0186 | 0.0376 | 0.0685 | 0.0315 | 0.0177 | 0.0254 | 0.0295 | 0.0148 | 0.0394 | 0.0355 |
Vacuole | 0.0011 | 0.0011 | 0.0021 | 0.0027 | 0.0008 | 0.0015 | 0.0077 | 0.0029 | 0.0033 | 0.0012 | 0.0012 | 0.0022 | 0.0035 | 0.002 | 0.0012 | 0.0012 | 0.0015 | 0.0011 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0006 | 0.0014 | 0.0002 | 0.0003 | 0.0031 | 0.0004 | 0.0013 | 0.0008 | 0.0003 | 0.0007 | 0.0014 | 0.0003 | 0.0007 | 0.0005 | 0.0018 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 364.0988 | 407.4962 | 430.0997 | 467.0105 | 392.0581 | 302.6309 | 433.5868 | 424.406 | 382.5467 | 414.6182 |
Translational Efficiency | 1.6498 | 1.7831 | 1.6595 | 1.3804 | 1.7202 | 2.1997 | 1.5968 | 1.5896 | 1.6242 | 1.5689 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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