Standard name
Description Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.05 0.06 0.07 0.23 0.33 0.32 0.18 0.20 0.18 0.25 0.13 0.13 0.15 0 0 0 0.15 0.28 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0.05 0.21 0.17 0.52 0.46 0.71 0.50 0.05 0 0 0 0 0 0 0 0
Nucleus 0.80 0.89 0.93 0.81 0.63 0.41 0.41 0.44 0.47 0.31 0.38 0.70 0.70 0.72 0.82 0.82 0.74 0.20 0.15 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0.13
Vac/Vac Membrane 0.06 0.10 0 0.09 0.12 0.19 0.17 0.08 0.10 0.08 0.10 0.20 0.17 0.17 0.09 0.10 0.16 0.23 0.33 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 1 2 0 4 1 0 0 0 0 4 3 0 0 1
Bud Neck 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 1 1 5 1 3 3 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 26 18 10 7 26 47 55 28 29 45 34 29 26 27 8 5 6 1 4 2
Endoplasmic Reticulum 1 0 1 0 0 0 0 1 0 0 2 13 4 1 1 0 1 0 0 0
Endosome 4 3 0 4 2 2 6 3 0 1 0 3 2 2 0 3 1 1 0 1
Golgi 0 0 0 1 0 0 1 0 0 0 0 0 1 0 1 3 0 0 0 0
Mitochondria 45 4 2 3 6 29 30 81 68 177 69 12 4 6 5 1 0 0 0 0
Nucleus 244 330 153 79 71 58 71 68 70 78 53 159 138 133 273 320 142 1 2 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0
Nucleolus 2 0 0 0 0 0 0 1 1 0 0 0 0 0 0 4 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 2 1 1 2 1 0 1 0 0 1 3 2 2 0 1 1
Vac/Vac Membrane 17 37 2 9 13 27 29 13 15 19 14 46 34 31 29 38 31 1 4 0
Unique Cell Count 305 370 164 98 113 141 174 155 148 248 138 227 197 184 332 389 193 7 15 14
Labelled Cell Count 341 392 168 103 120 165 196 205 185 328 178 262 209 201 332 389 193 7 15 14

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.9 5.7 5.5 5.9 4.7 5.1 4.2 4.3 3.8 4.0 7.2 7.2 7.0
Std Deviation (1e-4) 1.0 0.9 1.1 1.5 1.5 1.3 1.4 1.4 1.4 0.7 0.9 1.0 1.2 1.2
Intensity Change (Log2) -0.0 0.0 -0.3 -0.2 -0.4 -0.4 -0.6 -0.5 0.3 0.3 0.3

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 2.1 1.8 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 5.0 0 0 0 0 2.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.7 0 4.6 0 0 0 0 5.6 4.8 4.9