Standard name
Description Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.05 0.06 0.07 0.23 0.33 0.32 0.18 0.2 0.18 0.25 0.13 0.13 0.15 0 0 0 0.15 0.28 0.19
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0.05 0.21 0.17 0.52 0.46 0.71 0.5 0.05 0 0 0 0 0 0 0 0
Nucleus 0.8 0.89 0.93 0.81 0.63 0.41 0.41 0.44 0.47 0.31 0.38 0.7 0.7 0.72 0.82 0.82 0.74 0.2 0.15 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0.13
Vac/Vac Membrane 0.06 0.1 0 0.09 0.12 0.19 0.17 0.08 0.1 0.08 0.1 0.2 0.17 0.17 0.09 0.1 0.16 0.23 0.33 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 1 2 0 4 1 0 0 0 0 4 3 0 0 1
Bud Neck 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 1 1 5 1 3 3 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 26 18 10 7 26 47 55 28 29 45 34 29 26 27 8 5 6 1 4 2
Endoplasmic Reticulum 1 0 1 0 0 0 0 1 0 0 2 13 4 1 1 0 1 0 0 0
Endosome 4 3 0 4 2 2 6 3 0 1 0 3 2 2 0 3 1 1 0 1
Golgi 0 0 0 1 0 0 1 0 0 0 0 0 1 0 1 3 0 0 0 0
Mitochondria 45 4 2 3 6 29 30 81 68 177 69 12 4 6 5 1 0 0 0 0
Nucleus 244 330 153 79 71 58 71 68 70 78 53 159 138 133 273 320 142 1 2 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0
Nucleolus 2 0 0 0 0 0 0 1 1 0 0 0 0 0 0 4 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 2 1 1 2 1 0 1 0 0 1 3 2 2 0 1 1
Vac/Vac Membrane 17 37 2 9 13 27 29 13 15 19 14 46 34 31 29 38 31 1 4 0
Unique Cell Count 305 370 164 98 113 141 174 155 148 248 138 227 197 184 332 389 193 7 15 14
Labelled Cell Count 341 392 168 103 120 165 196 205 185 328 178 262 209 201 332 389 193 7 15 14


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.9 5.7 5.5 5.9 4.7 5.1 4.2 4.3 3.8 4.0 7.2 7.2 7.0
Std Deviation (1e-4) 1.0 0.9 1.1 1.5 1.5 1.3 1.4 1.4 1.4 0.7 0.9 1.0 1.2 1.2
Intensity Change (Log2) -0.05 0.03 -0.28 -0.18 -0.43 -0.4 -0.59 -0.52 0.33 0.33 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 4.1 6.1 5.9 3.3 3.6 3.5 4.5 2.2 2.2 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 5.0 0 0 0 0 2.1 0 0
Nucleus -3.1 -6.3 -9.8 -10.2 -9.6 -9.0 -12.4 -10.2 -5.6 -5.6 -5.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.7 5.3 4.9 3.0 3.5 2.9 3.4 5.7 5.1 5.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thi3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available