Standard name
Human Ortholog
Description SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.49 0.24 0.46 0.21 0.31 0.23 0.19 0.25 0.24 0.18 0.41 0.43 0.39 0.28 0.37 0.39 0.19 0.15 0.17 0.22 0.23 0.17
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 0 0 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.19 0.07 0.19 0.32 0.33 0.28 0.39 0.4 0.38 0.4 0.44 0.08 0.12 0.1 0.19 0.09 0.05 0.63 0.67 0.66 0.48 0.44 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.69 0.71 0.69 0.47 0.61 0.45 0.48 0.44 0.43 0.38 0.36 0.66 0.61 0.62 0.68 0.62 0.6 0 0.08 0.09 0.16 0.14 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 1
Bud 0 0 0 0 0 0 1 1 1 1 1 0 0 0 0 0 0 8 7 2 3 5 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 50 42 10 68 58 50 68 33 54 41 42 48 81 71 36 30 38 27 15 12 19 46 30
Endoplasmic Reticulum 0 0 0 1 0 2 0 1 0 0 1 4 0 1 5 5 5 0 0 0 1 2 3
Endosome 1 0 0 0 0 0 3 5 2 0 5 0 1 0 0 0 0 1 0 1 1 4 4
Golgi 1 0 0 0 2 0 2 0 1 0 0 5 0 3 5 5 5 1 0 0 0 0 0
Mitochondria 1 0 0 0 0 14 0 3 8 14 14 0 0 0 1 3 0 0 0 0 0 1 0
Nucleus 25 6 8 48 90 45 114 71 83 70 104 9 22 18 24 7 5 88 69 48 42 89 70
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 4 6
Nucleolus 1 0 0 0 0 0 1 2 1 0 2 1 0 0 0 0 1 1 0 0 1 6 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 0 0 0 4 6
Vac/Vac Membrane 90 61 29 69 167 71 141 78 92 66 87 78 114 111 86 51 59 4 8 6 14 27 29
Unique Cell Count 131 86 42 148 273 159 296 178 216 174 239 118 187 180 127 82 98 140 103 74 89 204 175
Labelled Cell Count 169 109 47 187 317 182 330 194 242 193 258 146 219 204 157 101 114 140 103 74 89 204 175


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.0 15.8 13.2 12.3 12.9 8.2 11.0 9.5 10.0 9.0 9.9 15.6 15.9 16.3 23.8 24.0 23.2 13.8 14.2 15.3
Std Deviation (1e-4) 2.9 2.5 3.4 2.0 1.9 2.1 1.9 1.9 1.8 1.7 2.9 2.7 3.0 2.7 6.2 6.7 6.6 2.8 2.6 2.6
Intensity Change (Log2) -0.1 -0.03 -0.69 -0.26 -0.47 -0.4 -0.55 -0.42 0.24 0.27 0.3 0.85 0.86 0.81 0.06 0.1 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.6 -0.4 1.0 -0.1 -0.8 0.2 0 -1.0 2.0 2.3 1.9 0.6 1.4 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.7 1.8 1.2 2.5 2.5 2.4 2.6 3.0 0 -1.3 0 0 -1.7 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 -1.0 -2.8 -2.6 -2.9 -3.1 -3.6 -4.0 -0.3 -1.0 -0.9 -0.2 -0.8 -1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9549 8.3466 6.0276 6.4548 7.0101 6.2221 8.4503 11.6111 10.3052 7.838 8.9502 10.088 9.0349 11.8159 10.6988 10.9397 9.3581 10.6989
Actin 0.0172 0.0002 0.0839 0.0011 0.0001 0.0042 0.0034 0.0063 0.0258 0.1604 0 0.0005 0.0423 0.0008 0.0152 0.0001 0.0759 0.0099
Bud 0.0033 0.0001 0.0003 0.0005 0 0.0001 0.0002 0.0042 0.0003 0.0021 0 0.0004 0.0005 0.0012 0.0002 0.0001 0.0005 0.001
Bud Neck 0.0057 0.0005 0.0028 0.0017 0.0005 0.0012 0.0002 0.0002 0.0002 0.0012 0.0001 0.0025 0.001 0.0003 0.0006 0.0003 0.0011 0.002
Bud Periphery 0.0023 0 0.0003 0.0003 0 0.0001 0.0001 0.0025 0.0004 0.0036 0 0.0002 0.0007 0.0009 0.0002 0 0.0006 0.0008
Bud Site 0.1168 0.0004 0.0031 0.0028 0 0.0003 0.0011 0.004 0.0004 0.0021 0 0.0001 0.0009 0.0069 0.0041 0 0.0026 0.0007
Cell Periphery 0.001 0.0001 0.0002 0.0001 0 0 0 0 0.0002 0.0002 0 0 0.0002 0.0002 0.0001 0 0.0001 0.0001
Cytoplasm 0.0787 0.1183 0.0447 0.0827 0.1066 0.0648 0.1207 0.1311 0.1954 0.0056 0.0025 0.1007 0.0943 0.0717 0.0975 0.0173 0.0173 0.0758
Cytoplasmic Foci 0.0717 0.0003 0.0155 0.027 0.0001 0.0058 0.0418 0.0033 0.0037 0.0124 0 0.0003 0.0028 0.0055 0.0031 0 0.012 0.0032
Eisosomes 0.0002 0 0.0013 0 0 0 0 0 0.0001 0.0004 0 0 0.0004 0 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.0044 0.002 0.01 0.0024 0.0001 0.002 0.0028 0.0005 0.0243 0.0026 0 0.002 0.0026 0.0006 0.0028 0.0001 0.0102 0.0042
Endosome 0.0453 0.0012 0.0159 0.0564 0 0.0213 0.0196 0.0003 0.0082 0.0329 0 0.0012 0.0068 0.0079 0.0089 0 0.0392 0.0286
Golgi 0.0283 0 0.0057 0.0071 0 0.005 0.0029 0.0004 0.0095 0.0446 0 0 0.0064 0.0038 0.0051 0 0.0036 0.0108
Lipid Particles 0.014 0 0.0056 0.0461 0 0.0112 0.0148 0 0.0018 0.0382 0 0.0001 0.0048 0.0002 0.0017 0 0.0107 0.0041
Mitochondria 0.012 0.0002 0.0012 0.0017 0.0001 0.0011 0.001 0.0279 0.0032 0.0414 0 0.0004 0.0534 0.0015 0.0175 0.0001 0.0014 0.004
None 0.0119 0.0005 0.0037 0.0029 0.0001 0.0009 0.009 0.0003 0.0009 0.0007 0 0.0003 0.0009 0.0002 0.0013 0 0.0029 0.0008
Nuclear Periphery 0.0384 0.0252 0.0451 0.0217 0.0081 0.0167 0.0185 0.0177 0.0304 0.0104 0.0047 0.0501 0.0252 0.0072 0.0106 0.0083 0.0273 0.0177
Nucleolus 0.0017 0.0024 0.0006 0.0018 0.0004 0.0007 0.0008 0.0004 0.0003 0.0005 0.0003 0.0018 0.0016 0.0015 0.0004 0.0004 0.0011 0.0007
Nucleus 0.4843 0.8238 0.7425 0.7175 0.8818 0.8559 0.7033 0.7961 0.6759 0.6289 0.9919 0.8325 0.7435 0.8605 0.8247 0.9717 0.7683 0.8245
Peroxisomes 0.0111 0 0.0061 0.004 0 0.0016 0.0142 0.0018 0.001 0.0076 0 0 0.0017 0.0003 0.0033 0 0.0029 0.0013
Punctate Nuclear 0.0442 0.0048 0.005 0.0113 0.0018 0.005 0.044 0.0012 0.0015 0.0018 0.0003 0.0048 0.0046 0.0007 0.0012 0.0011 0.0136 0.0063
Vacuole 0.0061 0.0064 0.0045 0.0097 0.0001 0.0012 0.0011 0.0013 0.0102 0.0011 0 0.0012 0.0032 0.0214 0.0007 0.0002 0.0067 0.0022
Vacuole Periphery 0.0012 0.0135 0.002 0.0013 0 0.0006 0.0005 0.0003 0.0062 0.0014 0 0.0007 0.0024 0.0066 0.001 0.0001 0.0017 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.8522 68.6718 63.3107 62.5321 78.4724 79.1033 70.486 70.2948 50.7777 79.3305
Translational Efficiency 2.0893 2.2629 1.6086 1.4725 1.768 1.8558 1.7383 1.5672 2.017 1.679

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
79 217 804 74 2540 1637 876 616 2619 1854 1680 690

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1173.35 1374.09 1688.31 1671.37 1170.07 1279.31 1614.96 1592.19 1170.17 1290.40 1650.06 1600.68
Standard Deviation 170.16 226.22 282.34 311.80 182.69 205.34 265.43 321.25 182.33 210.11 276.10 321.18
Intensity Change Log 2 0.227843 0.524946 0.510398 0.128771 0.464904 0.444418 0.179227 0.495279 0.477831

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001697 0.000633 0.000496 0.002064 0.000121 0.000357 0.000294 0.006151 0.000168 0.000389 0.000391 0.005713
Bud Neck 0.009620 0.006800 0.007500 0.016113 0.007376 0.008170 0.006031 0.017574 0.007443 0.008009 0.006734 0.017417
Bud Site 0.008273 0.002005 0.002061 0.032846 0.001651 0.004106 0.000967 0.020113 0.001850 0.003860 0.001490 0.021479
Cell Periphery 0.000518 0.000178 0.000132 0.000573 0.000147 0.000133 0.000105 0.001861 0.000158 0.000138 0.000118 0.001723
Cytoplasm 0.074989 0.083702 0.026619 0.029264 0.145044* 0.134137* 0.033452 0.117642* 0.142931* 0.128234* 0.030182 0.108164*
Cytoplasmic Foci 0.002838 0.000991 0.000218 0.015050 0.000496 0.002665 0.000117 0.010491 0.000567 0.002469 0.000165 0.010980
Eisosomes 0.000039 0.000014 0.000014 0.000024 0.000007 0.000008 0.000010 0.000469 0.000008 0.000009 0.000012 0.000421
Endoplasmic Reticulum 0.013366 0.002713 0.000997 0.001851 0.001997 0.002762 0.000696 0.003176 0.002340 0.002756 0.000840 0.003034
Endosome 0.004882 0.000259 0.000097 0.094573 0.000202 0.001927 0.000048 0.020938 0.000343 0.001732 0.000071 0.028835
Golgi 0.001294 0.000087 0.000055 0.108802 0.000030 0.001126 0.000035 0.033551 0.000068 0.001004 0.000044 0.041621
Lipid Particles 0.000864 0.000206 0.000099 0.000531 0.000082 0.000292 0.000052 0.000635 0.000105 0.000282 0.000074 0.000624
Mitochondria 0.001163 0.000655 0.001327 0.001746 0.000619 0.001508 0.000929 0.014692 0.000636 0.001408 0.001119 0.013304
Mitotic Spindle 0.006002 0.001858 0.004868 0.181829* 0.006265 0.008912 0.004790 0.188895* 0.006257 0.008086 0.004827 0.188138*
None 0.003940 0.006159 0.003910 0.009824 0.005318 0.006546 0.007881 0.014349 0.005277 0.006501 0.005981 0.013864
Nuclear Periphery 0.001154 0.000498 0.000624 0.000748 0.001152 0.001333 0.000489 0.004123 0.001152 0.001235 0.000554 0.003761
Nuclear Periphery Foci 0.002473 0.000744 0.000121 0.000464 0.000165 0.001161 0.000058 0.015261 0.000235 0.001112 0.000088 0.013674
Nucleolus 0.004650 0.001435 0.000194 0.000961 0.000288 0.000770 0.000126 0.001100 0.000420 0.000848 0.000159 0.001085
Nucleus 0.832088* 0.884074* 0.947980* 0.408039* 0.825207* 0.812924* 0.941812* 0.485871* 0.825414* 0.821252* 0.944764* 0.477524*
Peroxisomes 0.000241 0.000062 0.000050 0.000361 0.000023 0.000140 0.000015 0.000954 0.000030 0.000131 0.000032 0.000890
Vacuole 0.028424 0.006855 0.002597 0.067873 0.003694 0.009544 0.002070 0.029282 0.004440 0.009230 0.002322 0.033421
Vacuole Periphery 0.001485 0.000072 0.000042 0.026466 0.000116 0.001478 0.000026 0.012871 0.000158 0.001313 0.000033 0.014329

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.16 2.04 0.77 -2.98 -2.71 -6.29 -8.09 -2.21 -1.78 -1.65 -6.17 -8.64 -2.33 -1.88 -1.62
Bud Neck 0.89 -1.84 -3.05 -3.68 -2.35 -1.04 -4.75 -8.09 -6.94 -5.13 -1.07 -7.50 -8.84 -7.71 -4.91
Bud Site 1.79 1.27 -1.29 -2.99 -2.58 -4.09 -2.17 -6.95 -4.59 -6.49 -3.81 -2.93 -7.44 -5.36 -6.15
Cell Periphery 2.03 1.78 0.16 -3.89 -3.39 0.07 -1.46 -1.97 -1.97 -1.84 0.26 -2.40 -2.08 -2.10 -1.85
Cytoplasm -0.16 4.02 1.75 2.28 -1.93 2.27 27.55 10.16 7.76 -7.98 3.12 29.91 11.00 8.01 -8.71
Cytoplasmic Foci 1.89 2.73 0.49 -1.02 -1.65 -4.94 2.96 -3.76 -0.15 -4.34 -4.83 1.98 -3.88 -0.39 -4.35
Eisosomes 4.31 2.36 -0.56 -4.58 -2.84 -3.81 -10.45 -1.46 -1.42 -1.24 -3.21 -12.99 -1.53 -1.49 -1.20
Endoplasmic Reticulum 4.34 5.08 4.60 1.15 -2.74 -5.56 5.96 0.05 3.72 -3.14 -3.71 7.46 1.18 3.88 -3.28
Endosome 1.65 1.69 -0.67 -1.32 -1.33 -3.80 5.98 -2.96 -1.58 -3.06 -3.40 2.48 -3.10 -2.06 -3.26
Golgi 1.22 1.15 -0.96 -1.06 -1.06 -2.25 -7.99 -2.31 -1.88 -2.29 -2.19 -2.23 -2.53 -2.16 -2.51
Lipid Particles 3.46 3.21 0.28 -3.50 -3.22 -5.67 -4.29 -9.60 -6.92 -8.32 -5.32 -4.89 -9.99 -7.47 -8.45
Mitochondria 2.04 -3.84 -4.10 -6.17 -2.15 -2.41 -11.96 -2.82 -2.13 -2.18 -2.39 -18.81 -2.99 -2.31 -2.06
Mitotic Spindle 0.56 -1.04 -2.42 -2.89 -2.13 -0.35 -1.27 -8.89 -8.77 -8.18 -0.12 -3.59 -9.29 -9.24 -7.86
None -0.23 -0.14 -2.63 -2.89 -3.05 -0.78 -2.70 -7.86 -6.76 -5.23 -0.85 -2.49 -8.36 -7.26 -6.89
Nuclear Periphery 3.51 -0.01 -2.81 -4.59 -3.00 -1.25 0.86 -7.51 -6.42 -7.65 -0.70 -1.64 -8.05 -7.22 -7.56
Nuclear Periphery Foci 2.22 2.33 2.61 1.04 0.30 -3.50 2.72 -2.86 -1.84 -2.95 -3.33 -0.16 -2.78 -1.81 -2.70
Nucleolus 1.02 1.17 0.95 -0.54 -2.89 -2.25 -1.25 -6.56 -2.35 -5.65 -1.57 -0.29 -4.87 -2.39 -5.79
Nucleus -2.91 -3.85 1.92 4.68 5.47 1.48 -13.81 10.70 9.57 16.95 0.32 -14.33 11.05 10.58 17.54
Peroxisomes 3.09 2.54 1.33 -2.72 -1.75 -4.10 -1.72 -3.43 -1.67 -3.32 -3.97 -2.94 -3.55 -1.85 -3.02
Vacuole 3.13 2.60 -0.87 -4.78 -4.17 -5.77 -4.19 -11.28 -9.82 -9.36 -4.52 -5.12 -11.88 -10.69 -10.13
Vacuole Periphery 1.38 1.39 -0.59 -1.13 -1.14 -2.80 2.78 -3.07 -1.15 -3.18 -2.69 2.58 -3.22 -1.47 -3.36