Standard name | |
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Human Ortholog | |
Description | Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; regulates replication fork progression in DNA ligase I-deficient cells; paralog of Rad52p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 3 | 4 |
Bud | 0 | 0 | 0 | 0 | 2 | 4 | 1 | – | 5 | 1 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 9 | 12 |
Bud Neck | 0 | 0 | 5 | 1 | 1 | 5 | 5 | – | 10 | 5 | 8 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 4 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 0 | – | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 1 | 1 | 3 | 1 | – | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Cytoplasm | 166 | 387 | 261 | 187 | 140 | 109 | 203 | – | 189 | 124 | 160 | 180 | 260 | 432 | 357 | 42 | 39 | 15 | 166 | 343 | 225 | 185 | 254 | 315 |
Endoplasmic Reticulum | 1 | 0 | 3 | 0 | 0 | 3 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 4 | 3 | 0 | 8 | 0 | 1 | 2 | 3 | 3 |
Endosome | 5 | 1 | 0 | 1 | 1 | 0 | 5 | – | 1 | 4 | 2 | 0 | 0 | 7 | 8 | 6 | 1 | 0 | 9 | 3 | 3 | 3 | 5 | 5 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 3 | 0 | 0 | 1 | 0 |
Mitochondria | 6 | 5 | 0 | 6 | 6 | 76 | 49 | – | 167 | 95 | 123 | 169 | 1 | 4 | 2 | 5 | 2 | 0 | 22 | 18 | 18 | 16 | 25 | 11 |
Nucleus | 0 | 0 | 0 | 5 | 9 | 30 | 39 | – | 49 | 36 | 29 | 55 | 4 | 2 | 4 | 1 | 0 | 1 | 4 | 5 | 2 | 2 | 5 | 6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 2 | 3 | 6 | – | 3 | 2 | 7 | 9 | 1 | 1 | 1 | 0 | 0 | 2 | 4 | 8 | 7 | 10 | 9 | 14 |
Vac/Vac Membrane | 8 | 8 | 2 | 7 | 25 | 11 | 25 | – | 21 | 17 | 4 | 14 | 8 | 20 | 59 | 13 | 9 | 2 | 15 | 12 | 9 | 8 | 12 | 17 |
Unique Cell Count | 185 | 399 | 269 | 195 | 170 | 196 | 300 | 375 | 244 | 290 | 370 | 269 | 458 | 408 | 60 | 48 | 19 | 249 | 410 | 286 | 246 | 347 | 412 | |
Labelled Cell Count | 188 | 402 | 273 | 209 | 187 | 248 | 334 | 448 | 286 | 344 | 440 | 276 | 469 | 431 | 72 | 55 | 20 | 249 | 410 | 286 | 246 | 347 | 412 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 4.1 | 4.6 | 4.4 | 5.6 | 3.6 | 4.5 | – | 3.7 | 4.0 | 3.8 | 3.9 | 4.8 | 4.4 | 5.1 | 5.8 | 5.9 | 5.3 | 4.3 | 4.6 | 4.6 |
Std Deviation (1e-4) | 1.4 | 0.9 | 0.8 | 1.2 | 2.2 | 1.1 | 1.6 | – | 1.2 | 1.2 | 1.0 | 0.8 | 1.1 | 1.4 | 1.6 | 1.9 | 1.5 | 0.9 | 1.9 | 2.1 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.27 | -0.36 | -0.05 | – | -0.31 | -0.2 | -0.3 | -0.27 | 0.05 | -0.09 | 0.14 | 0.31 | 0.35 | 0.2 | -0.11 | -0.0 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.7544 | -1.3946 | -1.5586 | -1.7801 | -1.9938 | -1.4759 | 1.4303 | 0.0599 | 0.3527 | -0.0278 | -0.0032 | 0.3557 | 2.0112 | 3.0633 | 2.7292 | 2.7936 | 2.4055 | 2.8595 |
Actin | 0.0056 | 0.0003 | 0.0103 | 0.0074 | 0.0068 | 0.0007 | 0.1465 | 0.0001 | 0.005 | 0.0002 | 0.0005 | 0.0212 | 0.0002 | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.0006 |
Bud | 0.0003 | 0.0001 | 0.0022 | 0.0002 | 0.0004 | 0.0005 | 0.0018 | 0.004 | 0.0066 | 0.0487 | 0.0001 | 0.0002 | 0.0003 | 0.0012 | 0.0004 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0008 | 0.0003 | 0.0006 | 0.0004 | 0.0005 | 0.0007 | 0.0125 | 0.0001 | 0.0081 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0007 |
Bud Periphery | 0.0007 | 0.0001 | 0.001 | 0.0003 | 0.0012 | 0.0006 | 0.0036 | 0.0007 | 0.0116 | 0.0159 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.001 | 0 | 0 | 0.0001 |
Bud Site | 0.0023 | 0.0025 | 0.0052 | 0.0003 | 0.0002 | 0.0001 | 0.0248 | 0.0075 | 0.0054 | 0.0089 | 0.0001 | 0.0003 | 0.0002 | 0.0068 | 0.0006 | 0.0003 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0013 | 0.0004 | 0.0004 | 0.0016 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.1974 | 0.3301 | 0.2073 | 0.259 | 0.2554 | 0.3027 | 0.0672 | 0.2095 | 0.2189 | 0.1025 | 0.2062 | 0.2343 | 0.1995 | 0.2868 | 0.2364 | 0.2995 | 0.2541 | 0.3099 |
Cytoplasmic Foci | 0.0391 | 0.0241 | 0.0334 | 0.0515 | 0.0277 | 0.0225 | 0.0705 | 0.0064 | 0.0164 | 0.0085 | 0.0133 | 0.0069 | 0.0088 | 0.0129 | 0.0086 | 0.0037 | 0.0053 | 0.0066 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0012 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0044 | 0.0042 | 0.0031 | 0.0019 | 0.0038 | 0.0035 | 0.0026 | 0.0028 | 0.0036 | 0.0012 | 0.0033 | 0.0014 | 0.0023 | 0.0024 | 0.0018 | 0.0022 | 0.0009 | 0.0011 |
Endosome | 0.0201 | 0.009 | 0.0074 | 0.0046 | 0.0139 | 0.0048 | 0.0313 | 0.0038 | 0.0083 | 0.0016 | 0.0041 | 0.0086 | 0.0011 | 0.0021 | 0.0013 | 0.0022 | 0.0123 | 0.001 |
Golgi | 0.0034 | 0.0003 | 0.0023 | 0.0019 | 0.0037 | 0.0002 | 0.0199 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0158 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Lipid Particles | 0.0148 | 0.0027 | 0.0046 | 0.0024 | 0.011 | 0.0013 | 0.0372 | 0.0003 | 0.0015 | 0.0003 | 0.0003 | 0.0048 | 0.0005 | 0.0002 | 0.0002 | 0 | 0 | 0.0001 |
Mitochondria | 0.0023 | 0.0005 | 0.0039 | 0.005 | 0.0019 | 0.0004 | 0.0073 | 0.0003 | 0.0068 | 0.0005 | 0.0007 | 0.0366 | 0.0002 | 0.0069 | 0.0003 | 0.0006 | 0.0003 | 0.0002 |
None | 0.637 | 0.5691 | 0.6707 | 0.5288 | 0.6058 | 0.6105 | 0.505 | 0.7564 | 0.6613 | 0.801 | 0.7549 | 0.6558 | 0.7685 | 0.6671 | 0.7246 | 0.5784 | 0.6992 | 0.6479 |
Nuclear Periphery | 0.0147 | 0.0115 | 0.0042 | 0.0068 | 0.0226 | 0.0039 | 0.0027 | 0.0012 | 0.0051 | 0.0012 | 0.0024 | 0.0018 | 0.0014 | 0.0014 | 0.0011 | 0.0048 | 0.0008 | 0.0008 |
Nucleolus | 0.0016 | 0.0008 | 0.0015 | 0.0008 | 0.0014 | 0.0004 | 0.002 | 0.0002 | 0.0015 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0003 | 0.0005 | 0.0001 | 0.0003 |
Nucleus | 0.0194 | 0.0337 | 0.0262 | 0.0454 | 0.0212 | 0.0335 | 0.0012 | 0.0031 | 0.0098 | 0.003 | 0.009 | 0.0062 | 0.0059 | 0.0055 | 0.0095 | 0.1002 | 0.0068 | 0.0194 |
Peroxisomes | 0.0141 | 0.0006 | 0.0035 | 0.0235 | 0.0017 | 0.0006 | 0.0401 | 0.0001 | 0.0106 | 0.0001 | 0.0005 | 0.0016 | 0.0004 | 0.0007 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0168 | 0.0069 | 0.0105 | 0.0586 | 0.017 | 0.0113 | 0.0183 | 0.0012 | 0.0153 | 0.0014 | 0.0031 | 0.0025 | 0.0092 | 0.0028 | 0.0119 | 0.0055 | 0.0008 | 0.0101 |
Vacuole | 0.0029 | 0.0027 | 0.0014 | 0.0007 | 0.0021 | 0.0014 | 0.0025 | 0.0014 | 0.0021 | 0.0022 | 0.0007 | 0.0006 | 0.0007 | 0.0008 | 0.0006 | 0.0008 | 0.018 | 0.0004 |
Vacuole Periphery | 0.0014 | 0.0005 | 0.0004 | 0.0003 | 0.0015 | 0.0002 | 0.0007 | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.3862 | 8.2542 | 8.1666 | 7.4659 | 3.9905 | 7.6903 | 5.9116 | 8.9187 | 9.311 | 6.6271 |
Translational Efficiency | 1.5484 | 0.7595 | 0.9656 | 1.3837 | 1.4965 | 1.0859 | 1.3321 | 1.0196 | 1.0525 | 0.6571 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |