Standard name
Human Ortholog
Description Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; regulates replication fork progression in DNA ligase I-deficient cells; paralog of Rad52p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.97 0.97 0.96 0.82 0.56 0.68 – 0.5 0.51 0.55 0.49 0.97 0.94 0.88 0.7 0.81 0.79 0.67 0.84 0.79 0.75 0.73 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.39 0.16 – 0.45 0.39 0.42 0.46 0 0 0 0.08 0 0 0.09 0 0.06 0.07 0.07 0
Nucleus 0 0 0 0 0.05 0.15 0.13 – 0.13 0.15 0.1 0.15 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.15 0.06 0.08 – 0.06 0.07 0 0 0 0 0.14 0.22 0.19 0.11 0.06 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 1 0 0 0 0 0 0 0 0 0 2 1 2 1 3 4
Bud 0 0 0 0 2 4 1 – 5 1 6 3 0 0 0 0 0 0 1 3 0 2 9 12
Bud Neck 0 0 5 1 1 5 5 – 10 5 8 4 0 2 0 0 0 0 0 2 0 1 4 8
Bud Site 0 0 0 0 0 3 0 – 0 1 2 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 1 1 3 1 – 0 0 2 0 1 0 0 0 1 0 0 0 0 1 1 1
Cytoplasm 166 387 261 187 140 109 203 – 189 124 160 180 260 432 357 42 39 15 166 343 225 185 254 315
Endoplasmic Reticulum 1 0 3 0 0 3 0 – 1 0 0 1 1 0 0 4 3 0 8 0 1 2 3 3
Endosome 5 1 0 1 1 0 5 – 1 4 2 0 0 7 8 6 1 0 9 3 3 3 5 5
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 5 3 0 0 1 0
Mitochondria 6 5 0 6 6 76 49 – 167 95 123 169 1 4 2 5 2 0 22 18 18 16 25 11
Nucleus 0 0 0 5 9 30 39 – 49 36 29 55 4 2 4 1 0 1 4 5 2 2 5 6
Nuclear Periphery 0 0 0 0 0 0 0 – 1 1 0 1 0 0 0 0 0 0 1 0 0 0 2 1
Nucleolus 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 0 0 0 0 0 – 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 2 3 6 – 3 2 7 9 1 1 1 0 0 2 4 8 7 10 9 14
Vac/Vac Membrane 8 8 2 7 25 11 25 – 21 17 4 14 8 20 59 13 9 2 15 12 9 8 12 17
Unique Cell Count 185 399 269 195 170 196 300 375 244 290 370 269 458 408 60 48 19 249 410 286 246 347 412
Labelled Cell Count 188 402 273 209 187 248 334 448 286 344 440 276 469 431 72 55 20 249 410 286 246 347 412


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.1 4.6 4.4 5.6 3.6 4.5 – 3.7 4.0 3.8 3.9 4.8 4.4 5.1 5.8 5.9 5.3 4.3 4.6 4.6
Std Deviation (1e-4) 1.4 0.9 0.8 1.2 2.2 1.1 1.6 – 1.2 1.2 1.0 0.8 1.1 1.4 1.6 1.9 1.5 0.9 1.9 2.1 2.0
Intensity Change (Log2) – – – -0.06 0.27 -0.36 -0.05 – -0.31 -0.2 -0.3 -0.27 0.05 -0.09 0.14 0.31 0.35 0.2 -0.11 -0.0 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.7544 -1.3946 -1.5586 -1.7801 -1.9938 -1.4759 1.4303 0.0599 0.3527 -0.0278 -0.0032 0.3557 2.0112 3.0633 2.7292 2.7936 2.4055 2.8595
Actin 0.0056 0.0003 0.0103 0.0074 0.0068 0.0007 0.1465 0.0001 0.005 0.0002 0.0005 0.0212 0.0002 0.0005 0.0007 0.0002 0.0001 0.0006
Bud 0.0003 0.0001 0.0022 0.0002 0.0004 0.0005 0.0018 0.004 0.0066 0.0487 0.0001 0.0002 0.0003 0.0012 0.0004 0.0001 0 0.0002
Bud Neck 0.0008 0.0003 0.0006 0.0004 0.0005 0.0007 0.0125 0.0001 0.0081 0.0002 0.0001 0.0006 0.0001 0.0002 0.0001 0.0005 0.0001 0.0007
Bud Periphery 0.0007 0.0001 0.001 0.0003 0.0012 0.0006 0.0036 0.0007 0.0116 0.0159 0.0001 0.0003 0.0002 0.0006 0.001 0 0 0.0001
Bud Site 0.0023 0.0025 0.0052 0.0003 0.0002 0.0001 0.0248 0.0075 0.0054 0.0089 0.0001 0.0003 0.0002 0.0068 0.0006 0.0003 0.0001 0.0001
Cell Periphery 0.0003 0.0001 0.0002 0 0.0001 0 0.0013 0.0004 0.0004 0.0016 0 0.0001 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.1974 0.3301 0.2073 0.259 0.2554 0.3027 0.0672 0.2095 0.2189 0.1025 0.2062 0.2343 0.1995 0.2868 0.2364 0.2995 0.2541 0.3099
Cytoplasmic Foci 0.0391 0.0241 0.0334 0.0515 0.0277 0.0225 0.0705 0.0064 0.0164 0.0085 0.0133 0.0069 0.0088 0.0129 0.0086 0.0037 0.0053 0.0066
Eisosomes 0.0003 0.0001 0.0002 0.0002 0.0002 0.0001 0.0012 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0044 0.0042 0.0031 0.0019 0.0038 0.0035 0.0026 0.0028 0.0036 0.0012 0.0033 0.0014 0.0023 0.0024 0.0018 0.0022 0.0009 0.0011
Endosome 0.0201 0.009 0.0074 0.0046 0.0139 0.0048 0.0313 0.0038 0.0083 0.0016 0.0041 0.0086 0.0011 0.0021 0.0013 0.0022 0.0123 0.001
Golgi 0.0034 0.0003 0.0023 0.0019 0.0037 0.0002 0.0199 0.0001 0.0008 0.0001 0.0002 0.0158 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Lipid Particles 0.0148 0.0027 0.0046 0.0024 0.011 0.0013 0.0372 0.0003 0.0015 0.0003 0.0003 0.0048 0.0005 0.0002 0.0002 0 0 0.0001
Mitochondria 0.0023 0.0005 0.0039 0.005 0.0019 0.0004 0.0073 0.0003 0.0068 0.0005 0.0007 0.0366 0.0002 0.0069 0.0003 0.0006 0.0003 0.0002
None 0.637 0.5691 0.6707 0.5288 0.6058 0.6105 0.505 0.7564 0.6613 0.801 0.7549 0.6558 0.7685 0.6671 0.7246 0.5784 0.6992 0.6479
Nuclear Periphery 0.0147 0.0115 0.0042 0.0068 0.0226 0.0039 0.0027 0.0012 0.0051 0.0012 0.0024 0.0018 0.0014 0.0014 0.0011 0.0048 0.0008 0.0008
Nucleolus 0.0016 0.0008 0.0015 0.0008 0.0014 0.0004 0.002 0.0002 0.0015 0.0006 0.0003 0.0001 0.0002 0.0007 0.0003 0.0005 0.0001 0.0003
Nucleus 0.0194 0.0337 0.0262 0.0454 0.0212 0.0335 0.0012 0.0031 0.0098 0.003 0.009 0.0062 0.0059 0.0055 0.0095 0.1002 0.0068 0.0194
Peroxisomes 0.0141 0.0006 0.0035 0.0235 0.0017 0.0006 0.0401 0.0001 0.0106 0.0001 0.0005 0.0016 0.0004 0.0007 0.0002 0.0001 0.0001 0.0001
Punctate Nuclear 0.0168 0.0069 0.0105 0.0586 0.017 0.0113 0.0183 0.0012 0.0153 0.0014 0.0031 0.0025 0.0092 0.0028 0.0119 0.0055 0.0008 0.0101
Vacuole 0.0029 0.0027 0.0014 0.0007 0.0021 0.0014 0.0025 0.0014 0.0021 0.0022 0.0007 0.0006 0.0007 0.0008 0.0006 0.0008 0.018 0.0004
Vacuole Periphery 0.0014 0.0005 0.0004 0.0003 0.0015 0.0002 0.0007 0.0002 0.0006 0.0003 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.3862 8.2542 8.1666 7.4659 3.9905 7.6903 5.9116 8.9187 9.311 6.6271
Translational Efficiency 1.5484 0.7595 0.9656 1.3837 1.4965 1.0859 1.3321 1.0196 1.0525 0.6571

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘