Standard name
Description Transcription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.05 0.11 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.05 0.05 0.05 0 0 0 0 0.1 0.1 0.12 0.11 0 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.16 0.05 0.05 0.05 0.24 0.29 0.17 0.5 0.57 0.56 0.29 0.05 0 0 0 0.05 0.2 0 0 0 0 0 0
Nucleus 0.94 0.88 0.95 0.94 0.89 0.84 0.82 0.83 0.78 0.74 0.8 0.79 0.86 0.85 0.8 0.77 0.86 0.72 0.83 0.83 0.81 0.57 0.55 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0.06 0 0.05 0.12 0.12 0.21 0.12 0.09 0.05 0.08 0 0.12 0.08 0.08 0.06 0.06 0.21 0.19 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0 0.07 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0.06 0.1 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2
Bud 0 0 1 0 1 0 5 2 6 8 3 6 0 0 0 1 0 0 0 2 3 1 4 10
Bud Neck 0 0 2 1 2 3 2 5 1 3 3 4 0 0 0 0 0 0 0 0 1 1 2 4
Bud Site 0 0 1 5 2 16 18 6 18 22 25 14 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 9 12 7 19 20 26 23 11 10 7 9 31 25 37 8 1 3 0 2 2 1 2 2
Endoplasmic Reticulum 0 2 1 0 0 1 0 0 0 0 0 0 1 0 2 1 1 1 0 0 0 1 2 3
Endosome 0 1 1 0 1 0 1 0 1 0 0 0 0 4 3 0 1 2 2 3 2 3 11 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 3 0 0 1 2
Mitochondria 24 41 20 11 16 101 142 72 200 230 133 73 17 1 7 3 3 5 3 2 3 4 5 4
Nucleus 205 221 372 204 308 348 403 352 308 300 190 201 273 210 237 55 49 18 187 222 334 120 159 145
Nuclear Periphery 1 0 3 0 2 8 8 2 6 6 1 2 8 1 0 1 0 0 0 0 1 1 1 2
Nucleolus 18 5 3 4 6 23 21 22 48 49 50 30 29 12 23 1 7 2 18 16 25 44 55 51
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0 1
SpindlePole 2 3 28 8 23 8 22 29 10 10 5 6 2 4 2 1 0 0 0 2 7 4 6 14
Vac/Vac Membrane 0 5 2 0 5 2 3 3 1 2 0 1 2 4 6 4 3 1 7 7 24 20 30 27
Unique Cell Count 219 252 392 217 348 413 492 422 397 407 237 255 318 247 297 71 57 25 226 267 411 212 290 299
Labelled Cell Count 253 287 446 240 385 530 651 516 610 640 417 346 365 265 317 75 65 32 226 267 411 212 290 299


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.4 5.5 5.2 5.7 4.6 4.8 5.1 4.1 3.9 3.8 4.5 5.9 6.8 5.9 7.7 8.2 7.2 6.2 5.3 5.6
Std Deviation (1e-4) 0.8 0.8 0.8 1.3 1.2 1.3 1.2 1.3 0.7 0.7 1.0 1.1 2.3 4.1 2.0 2.2 1.5 1.7 4.4 2.0 1.7
Intensity Change (Log2) – – – -0.09 0.05 -0.26 -0.2 -0.13 -0.44 -0.5 -0.55 -0.31 0.09 0.29 0.09 0.46 0.56 0.37 0.17 -0.05 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 1.6 1.3 1.6 1.7 -0.2 -0.5 -0.1 0.3 3.7 3.7 4.8 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -0.3 0 0 5.4 0 0 0 0 0.1 -3.2 -1.8 0 0 0
Nucleus -0.5 -3.2 -4.9 -5.8 -5.2 -7.0 -8.2 -5.8 -6.3 -4.2 -4.3 -6.1 -5.0 -2.6 -4.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 3.7 0 0 0 0 5.3 3.3 4.8 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.7 -0.3 0 0 -0.2 0 0 0 0 -4.3 -3.1 -4.1 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0