Standard name
Description RNA binding protein required for maturation of tRNA and U6 snRNA; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.07 0 0.11 0.05 0.12 0.06 0 0 0 0.06 0.05 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.97 0.98 0.95 0.94 0.91 0.95 0.92 0.9 0.9 0.89 0.91 0.87 0.92 0.92 0.84 0.88 0.84 0.89 0.96 0.95 0.95 0.82 0.75 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.06 0.1 0.18 0.16 0.18 0.16 0 0 0.07 0.06 0.08 0.05 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 12 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 0 0 0 0 0 1 4 2 2 4 0 2 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 0 2 11 15 1 15 10 6 5 2 7 9 14 28 3 13 10 0 1 0 11 20 15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 3 0 1
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 2
Mitochondria 0 2 0 1 0 2 2 1 8 9 5 10 0 1 0 4 2 7 0 0 0 1 8 3
Nucleus 74 126 191 242 255 369 434 469 244 354 358 378 119 292 217 56 92 149 87 126 201 160 310 237
Nuclear Periphery 0 0 0 0 0 0 2 1 0 0 4 2 0 1 1 0 0 0 0 0 0 0 0 0
Nucleolus 3 1 5 1 5 23 29 54 48 62 70 71 1 14 18 4 9 8 0 0 3 1 5 19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 1 0 4 5 0 0 1 0 0 0 1 9 12
Vac/Vac Membrane 1 0 4 3 6 4 11 9 0 9 1 1 0 1 4 1 1 5 3 3 4 5 35 53
Unique Cell Count 76 128 200 257 281 390 471 523 272 399 394 433 129 316 258 64 109 168 91 134 212 195 416 364
Labelled Cell Count 79 129 202 259 282 399 493 546 310 441 444 475 131 331 275 68 117 182 91 134 212 195 416 364


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.0 16.4 21.6 20.7 19.4 14.9 14.3 13.7 11.8 12.5 12.1 12.0 20.7 20.1 17.9 28.5 30.6 34.2 21.0 19.2 19.6
Std Deviation (1e-4) 6.3 4.3 6.1 7.2 5.8 4.0 4.1 3.5 3.6 3.9 3.5 3.4 7.1 7.4 7.4 13.6 16.7 16.7 9.8 9.5 9.2
Intensity Change (Log2) – – – -0.06 -0.16 -0.53 -0.6 -0.65 -0.87 -0.79 -0.83 -0.84 -0.06 -0.1 -0.27 0.4 0.5 0.66 -0.04 -0.17 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 2.5 0 1.6 0 0 0 0 0 0 2.2 4.2 0 4.3 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -0.6 -2.0 -0.5 -1.6 -2.5 -2.3 -2.7 -2.0 -3.2 -1.2 -1.4 -3.9 -2.3 -3.4 -2.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.8 2.0 3.4 5.2 4.8 5.3 5.0 0 1.1 2.2 0 0 1.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.439 23.2268 25.3704 22.4783 23.7446 22.7513 21.5156 24.2515 21.1342 16.6974 18.3869 21.2808 19.2823 23.4056 20.493 18.6942 16.7406 19.1737
Actin 0.0365 0 0.0085 0 0.0027 0.0018 0 0 0.001 0.0149 0.0166 0 0.0242 0 0.0097 0.0032 0.0153 0.0116
Bud 0.0006 0 0 0 0.0001 0.0001 0 0 0.0001 0.0002 0.0004 0 0.0006 0 0.0001 0.0007 0.0004 0.0001
Bud Neck 0.0006 0 0.0001 0 0.0001 0.0003 0 0 0.0001 0.0001 0.0003 0.0003 0.0005 0 0.0001 0.0001 0.0006 0.0007
Bud Periphery 0.001 0 0.0001 0 0.0001 0.0001 0 0 0.0001 0.0001 0.0005 0.0001 0.0007 0 0.0001 0.0016 0.0007 0.0002
Bud Site 0.002 0 0.0002 0 0.0001 0.0001 0 0 0.0004 0.0004 0.0005 0 0.0032 0 0.0002 0.0006 0.0012 0.0001
Cell Periphery 0.0008 0 0 0.0003 0 0.0001 0 0 0 0 0.0001 0.0001 0.0004 0 0.0001 0.0011 0.0009 0.0002
Cytoplasm 0.001 0 0 0 0 0 0 0 0.0015 0.0104 0.0005 0 0.0142 0 0.001 0.0029 0.0022 0.0008
Cytoplasmic Foci 0.004 0 0.0014 0 0.0006 0.0002 0 0 0.0078 0.0133 0.0039 0 0.0167 0 0.0048 0.0012 0.0297 0.003
Eisosomes 0.0011 0 0.0001 0 0.0001 0 0 0 0 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0.0004 0
Endoplasmic Reticulum 0.0007 0 0.0001 0 0 0 0 0 0.0004 0.002 0.0006 0 0.0029 0 0.0009 0.001 0.0013 0.0005
Endosome 0.004 0 0.0004 0 0.0002 0.0009 0 0 0.0047 0.0085 0.0147 0 0.0221 0 0.0031 0.0042 0.0161 0.005
Golgi 0.0038 0 0.0004 0 0.001 0.0015 0 0 0.0051 0.0017 0.0178 0 0.0123 0 0.0045 0.0003 0.0084 0.0019
Lipid Particles 0.0116 0 0.0036 0 0.01 0.0005 0 0 0.0026 0.0023 0.0012 0 0.0129 0 0.002 0.0006 0.028 0.0106
Mitochondria 0.0264 0.0006 0.0027 0.0021 0.072 0.0034 0.0011 0.0004 0.0041 0.0059 0.0404 0.0004 0.0173 0.0004 0.0018 0.0131 0.0569 0.0065
None 0.0168 0 0.0003 0 0 0 0 0 0.0008 0.0045 0.0002 0 0.0048 0 0.0012 0.0038 0.003 0.0005
Nuclear Periphery 0.0024 0.0019 0.0014 0.0005 0.0003 0.0002 0.0007 0.0006 0.0086 0.038 0.0007 0.0012 0.007 0.0005 0.0004 0.0161 0.0042 0.0038
Nucleolus 0.0402 0.0146 0.0137 0.0443 0.0827 0.0495 0.0089 0.0038 0.0084 0.004 0.0921 0.0294 0.0455 0.0045 0.0063 0.0093 0.0996 0.0416
Nucleus 0.8303 0.9821 0.9652 0.9498 0.8038 0.9407 0.9888 0.995 0.9345 0.86 0.8064 0.9677 0.7935 0.9943 0.9623 0.9322 0.6874 0.9081
Peroxisomes 0.0075 0 0.001 0 0.0256 0.0002 0 0 0.0024 0.0055 0.0014 0 0.0055 0 0.0003 0.0005 0.0133 0.0008
Punctate Nuclear 0.0025 0 0.0005 0 0 0 0 0 0.0164 0.0267 0.0002 0 0.0119 0 0.0001 0.0042 0.0086 0.0015
Vacuole 0.0028 0.0001 0.0002 0.0007 0.0001 0.0002 0.0001 0 0.0003 0.0004 0.0005 0.0004 0.0017 0 0.0004 0.0013 0.0063 0.001
Vacuole Periphery 0.0034 0.0004 0.0003 0.0021 0.0004 0.0002 0.0002 0.0001 0.0006 0.001 0.0009 0.0003 0.0017 0.0001 0.0005 0.0018 0.0155 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 208.5541 216.8122 214.8066 144.4465 180.5543 235.7421 134.0003 117.0655 109.6959 130.857
Translational Efficiency 1.6835 1.7231 1.3396 1.8814 1.6443 1.1594 1.574 1.8283 1.4822 1.6957

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘