Standard name
Human Ortholog
Description Subunit of the SF3a splicing factor complex; required for spliceosome assembly

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.09 0.06 0.1 0.05 0.13 0.07 0.05 0.07 0.08 0.06 0.06 0.12 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0
Mitochondria 0.05 0.12 0 0 0.26 0.14 0.59 0.22 0.58 0.42 0.41 0.54 0.08 0.06 0 0 0 0 0 0 0
Nucleus 0.9 0.92 0.9 0.9 0.82 0.87 0.62 0.8 0.73 0.74 0.67 0.65 0.86 0.85 0.86 0.84 0.9 0.83 0.79 0.62 0.57
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0 0.05 0 0.14 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 5 1 16 3 8 7 5 9 0 0 0 4 0 0 0 0 0
Bud Neck 0 0 0 0 1 0 2 1 0 2 0 1 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 1 2 4 14 6 13 10 7 24 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 18 8 21 18 37 25 52 42 26 40 26 33 13 17 15 2 2 3 0 0 0
Endoplasmic Reticulum 0 1 1 1 0 0 1 0 0 0 0 0 10 3 5 0 0 1 0 0 0
Endosome 2 0 0 3 1 3 0 3 1 0 4 0 2 2 0 4 2 2 0 0 2
Golgi 0 1 1 1 0 0 0 1 0 0 0 0 0 0 0 1 1 1 0 0 0
Mitochondria 22 27 3 5 98 65 232 134 300 244 135 286 17 9 2 2 1 3 0 0 0
Nucleus 370 209 217 276 313 398 244 489 376 435 221 339 172 118 115 356 210 199 3 6 9
Nuclear Periphery 3 0 0 3 1 2 0 0 5 3 3 5 2 0 1 1 0 1 0 0 0
Nucleolus 18 0 0 1 1 0 5 5 9 3 3 8 0 1 0 12 0 6 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0
SpindlePole 3 0 2 0 2 3 9 13 5 3 6 13 1 2 1 5 2 0 0 0 0
Vac/Vac Membrane 8 4 3 5 5 16 2 23 7 10 15 13 9 2 6 27 9 12 0 1 2
Unique Cell Count 409 228 242 307 380 458 391 614 518 586 328 525 201 139 134 423 234 241 5 10 16
Labelled Cell Count 444 250 248 314 466 517 577 721 750 757 425 731 226 155 145 423 234 241 5 10 16


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 5.0 7.0 6.3 5.0 5.1 3.3 5.0 4.2 4.2 4.7 4.2 5.4 7.1 7.3
Std Deviation (1e-4) 1.0 0.7 2.0 1.9 1.6 1.4 1.0 1.2 1.3 1.0 1.8 0.9 1.1 1.9 1.3
Intensity Change (Log2) -0.15 -0.48 -0.44 -1.06 -0.48 -0.73 -0.72 -0.57 -0.72 -0.36 0.03 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 0.4 -1.6 1.8 -0.9 -2.0 -0.9 -0.3 -1.2 -0.9 1.1 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 8.1 5.5 0 7.4 0 0 0 0 3.6 0 0
Nucleus 0.1 -2.5 -1.1 -7.5 -3.5 -5.3 -4.9 -6.2 -7.2 -1.3 -1.4 -1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.7 0 1.9 0 0 2.2 1.1 2.1 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0421 1.0037 0.7634 -0.0845 0.5393 0.4035 5.2013 5.6334 5.501 5.5373 5.6884 5.5024 3.261 3.1395 2.606 3.0493 2.9616 2.7476
Actin 0.081 0 0.0026 0 0.0193 0.0035 0.0133 0 0.0003 0 0.0026 0.0002 0.0143 0 0.0008 0.0087 0.0116 0.0008
Bud 0.0007 0 0.0003 0 0.001 0 0.0002 0 0 0 0 0.0001 0.0002 0 0 0.0002 0.0002 0.0001
Bud Neck 0.0013 0.0001 0.0011 0.0001 0.0011 0.0005 0.0051 0 0.0005 0 0.0004 0.0017 0.0011 0.0001 0.0002 0.0004 0.0005 0.0013
Bud Periphery 0.0012 0.0001 0.0008 0 0.0015 0 0.0003 0 0.0001 0 0 0.0003 0.0003 0 0.0001 0.0002 0.0002 0.0002
Bud Site 0.006 0.0002 0.0033 0 0.0022 0 0.0046 0 0.0002 0 0.0001 0.0004 0.0006 0 0.0002 0.001 0.0004 0.0001
Cell Periphery 0.0005 0 0.0003 0 0.0004 0 0.0002 0 0 0 0 0.0001 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0027 0.0005 0.0037 0.0003 0.0106 0.0001 0.0022 0.0001 0.0058 0.0005 0.0029 0.0003 0.0141 0.0001 0.0004 0.0121 0.0011 0.0002
Cytoplasmic Foci 0.0193 0 0.0077 0 0.0174 0.0004 0.0116 0 0.0007 0 0.0025 0.0001 0.0178 0 0.0041 0.0047 0.0019 0.0001
Eisosomes 0.0012 0 0.0001 0 0.0003 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0033 0.0001 0.0021 0 0.0044 0.0001 0.0025 0 0.001 0 0.0032 0.0005 0.0044 0 0.0001 0.0059 0.0017 0.0001
Endosome 0.0349 0.0001 0.0061 0 0.0798 0.0009 0.0046 0 0.0015 0 0.0045 0.0005 0.017 0 0.0015 0.0186 0.0178 0.0005
Golgi 0.018 0 0.0016 0 0.0441 0.0009 0.0022 0 0.0001 0 0.0008 0 0.0032 0 0.0007 0.0015 0.0056 0.0001
Lipid Particles 0.0382 0 0.0047 0 0.0382 0.004 0.0083 0 0.0002 0 0.0041 0 0.011 0 0.0044 0.0021 0.005 0.0002
Mitochondria 0.0183 0.0005 0.0065 0.0002 0.0218 0.0007 0.0011 0.0001 0.0005 0.0001 0.0005 0.0009 0.0017 0.0001 0.0008 0.001 0.0045 0.0016
None 0.0169 0.0003 0.001 0.0001 0.0128 0.0004 0.0115 0 0.0032 0.0002 0.0107 0.0001 0.0078 0 0.0005 0.0057 0.0013 0.0001
Nuclear Periphery 0.0447 0.0145 0.0077 0.0059 0.0262 0.0064 0.0193 0.0007 0.0064 0.0029 0.0167 0.0036 0.0298 0.0006 0.0026 0.0214 0.0142 0.0024
Nucleolus 0.0071 0.0133 0.0079 0.0055 0.0134 0.0271 0.0054 0.0068 0.0046 0.0054 0.0244 0.0219 0.0077 0.0068 0.011 0.0113 0.029 0.0169
Nucleus 0.6705 0.9607 0.9216 0.8882 0.6728 0.9521 0.8824 0.992 0.9679 0.9903 0.9113 0.9665 0.8427 0.991 0.9475 0.8901 0.8863 0.973
Peroxisomes 0.0195 0 0.0019 0 0.0072 0.0018 0.0109 0 0 0 0.0005 0 0.0054 0 0.0022 0.0006 0.0013 0.0002
Punctate Nuclear 0.0101 0.0093 0.0169 0.0996 0.0114 0.0007 0.0128 0.0001 0.0063 0.0006 0.014 0.0018 0.0176 0.0012 0.0222 0.0127 0.0159 0.0017
Vacuole 0.0021 0.0002 0.0015 0.0001 0.0083 0.0001 0.0008 0 0.0005 0 0.0003 0.0007 0.0017 0 0.0002 0.0011 0.0005 0.0002
Vacuole Periphery 0.0025 0.0001 0.0005 0.0001 0.0058 0.0001 0.0006 0 0.0002 0 0.0003 0.0002 0.0014 0 0.0002 0.0007 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5764 8.62 5.4826 11.1383 8.0371 9.6374 5.6029 6.3027 8.1152 12.084
Translational Efficiency 3.2117 1.1146 3.2638 1.5044 2.8261 2.5569 2.0177 2.5711 2.2107 1.6178

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
419 1400 2719 1746 2063 2109 2437 1400 2482 3509 5156 3146

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 661.88 829.89 906.97 977.23 749.38 880.98 950.88 939.82 734.61 860.60 927.72 960.58
Standard Deviation 103.73 117.89 132.39 146.62 94.77 119.13 122.86 136.61 101.76 121.24 129.84 143.46
Intensity Change Log 2 0.326350 0.454485 0.562128 0.233412 0.343566 0.326687 0.277746 0.396650 0.441904

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000147 0.000664 0.000418 0.000824 0.000052 0.000237 0.000440 0.000441 0.000068 0.000408 0.000428 0.000654
Bud Neck 0.001927 0.009360 0.018498 0.024165 0.002867 0.005962 0.020431 0.019431 0.002708 0.007318 0.019412 0.022058
Bud Site 0.000920 0.001210 0.001710 0.005094 0.000196 0.001083 0.001037 0.001039 0.000318 0.001134 0.001392 0.003289
Cell Periphery 0.000209 0.000102 0.000062 0.000113 0.000071 0.000112 0.000063 0.000048 0.000095 0.000108 0.000062 0.000084
Cytoplasm 0.022347 0.006996 0.002596 0.007121 0.011150 0.006935 0.001004 0.001424 0.013040 0.006959 0.001844 0.004586
Cytoplasmic Foci 0.006191 0.000884 0.000194 0.002441 0.000279 0.001129 0.000139 0.000502 0.001277 0.001031 0.000168 0.001578
Eisosomes 0.000029 0.000021 0.000018 0.000025 0.000006 0.000015 0.000025 0.000019 0.000010 0.000017 0.000021 0.000022
Endoplasmic Reticulum 0.002149 0.007475 0.003174 0.005743 0.001271 0.003702 0.003964 0.004013 0.001419 0.005207 0.003548 0.004973
Endosome 0.001689 0.000498 0.000425 0.003330 0.000152 0.000577 0.000235 0.001036 0.000411 0.000545 0.000335 0.002310
Golgi 0.000129 0.000154 0.000192 0.002089 0.000023 0.000289 0.000215 0.000465 0.000041 0.000235 0.000203 0.001367
Lipid Particles 0.002304 0.000428 0.000046 0.000580 0.000068 0.000483 0.000035 0.000113 0.000445 0.000461 0.000041 0.000372
Mitochondria 0.001357 0.002579 0.001136 0.005242 0.000313 0.002484 0.001490 0.001590 0.000489 0.002522 0.001304 0.003617
Mitotic Spindle 0.000092 0.003445 0.001020 0.025978 0.000311 0.004730 0.001738 0.005328 0.000274 0.004217 0.001359 0.016788
None 0.021125 0.005940 0.002125 0.005432 0.004818 0.005682 0.002714 0.001719 0.007571 0.005785 0.002403 0.003780
Nuclear Periphery 0.000551 0.001735 0.000593 0.004851 0.000589 0.001582 0.000629 0.001504 0.000583 0.001643 0.000610 0.003362
Nuclear Periphery Foci 0.000280 0.000605 0.000142 0.001855 0.000063 0.000608 0.000077 0.000349 0.000100 0.000607 0.000111 0.001185
Nucleolus 0.017712 0.017265 0.006579 0.005671 0.005639 0.012187 0.008507 0.013821 0.007678 0.014213 0.007490 0.009298
Nucleus 0.918474* 0.937039* 0.960215* 0.884327* 0.971825* 0.950461* 0.956397* 0.945219* 0.962819* 0.945106* 0.958410* 0.911425*
Peroxisomes 0.001194 0.001250 0.000086 0.000584 0.000044 0.000446 0.000109 0.000140 0.000238 0.000767 0.000097 0.000386
Vacuole 0.000959 0.001855 0.000624 0.010122 0.000204 0.000907 0.000574 0.001307 0.000331 0.001285 0.000600 0.006199
Vacuole Periphery 0.000215 0.000495 0.000146 0.004411 0.000059 0.000389 0.000179 0.000492 0.000086 0.000432 0.000161 0.002667

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.70 -4.78 -10.59 -3.03 -8.49 -3.52 -23.37 -15.12 -5.23 -1.77 -6.92 -21.13 -20.15 -6.61 -8.39
Bud Neck -8.77 -19.97 -18.00 -11.59 -3.89 -5.89 -21.47 -15.55 -12.40 0.27 -10.33 -29.90 -23.90 -17.54 -2.76
Bud Site -0.36 -1.29 -4.15 -4.65 -3.33 -3.99 -9.68 -6.38 -1.34 -1.28 -4.93 -5.46 -7.22 -4.81 -3.61
Cell Periphery 2.43 3.41 2.54 0.34 -3.97 -1.97 0.66 1.32 3.51 1.20 -0.84 2.37 1.14 2.59 -2.66
Cytoplasm 4.34 5.84 4.20 -0.43 -4.56 2.59 7.64 6.83 5.62 -2.86 4.22 8.81 5.74 2.08 -5.17
Cytoplasmic Foci 3.29 3.85 3.11 -0.52 -2.62 -2.59 1.19 -0.63 2.04 -1.35 0.49 3.96 1.21 0.73 -2.94
Eisosomes 2.90 3.89 -0.06 -5.25 -8.50 -8.31 -23.64 -15.79 -4.70 5.32 -7.61 -16.01 -16.14 -8.03 -4.02
Endoplasmic Reticulum -10.14 -3.73 -11.23 2.07 -11.58 -11.25 -14.94 -14.12 -2.67 -1.53 -16.75 -18.62 -22.93 -1.41 -9.60
Endosome 1.02 1.09 -0.07 -4.05 -4.07 -4.32 -1.47 -2.06 -0.84 -1.80 -0.49 0.46 -3.08 -3.56 -4.35
Golgi -0.47 -0.50 -2.79 -2.72 -2.55 -2.75 -1.29 -2.13 -0.58 -0.92 -3.25 -1.83 -3.62 -2.58 -2.69
Lipid Particles 2.42 2.90 2.45 0.29 -2.70 -4.18 1.97 -1.68 3.41 -2.49 -0.08 3.00 1.17 1.97 -3.17
Mitochondria -2.33 0.31 -4.25 -2.18 -4.62 -3.84 -4.57 -6.92 1.00 -0.68 -5.25 -6.12 -7.35 -1.30 -4.44
Mitotic Spindle -2.87 -3.76 -7.78 -5.93 -7.31 -3.84 -3.15 -4.25 -1.70 -2.81 -4.68 -3.89 -8.77 -5.83 -7.64
None 4.00 5.04 4.33 1.28 -3.58 -1.01 2.87 4.11 4.43 1.01 1.74 6.01 4.49 3.45 -2.51
Nuclear Periphery -5.12 -1.56 -5.95 -3.86 -5.74 -3.38 -1.50 -3.10 -0.52 -2.64 -5.33 -2.09 -6.69 -3.57 -6.30
Nuclear Periphery Foci -0.10 1.31 -2.23 -2.73 -4.28 -3.71 -1.13 -2.59 1.03 -2.40 -3.93 0.27 -4.60 -2.08 -4.82
Nucleolus 0.26 5.27 5.58 8.54 0.72 -6.69 -5.78 -8.01 -1.91 -4.02 -8.06 -2.00 -4.12 3.84 -2.53
Nucleus -2.33 -4.78 2.41 7.20 11.83 6.29 8.14 9.44 3.34 3.99 5.91 3.91 13.38 8.45 12.07
Peroxisomes -0.04 2.69 1.95 1.82 -1.92 -4.15 -5.62 -6.94 2.99 -1.94 -2.56 1.81 -0.32 2.37 -2.12
Vacuole -0.86 1.24 -6.63 -6.50 -7.65 -3.09 -5.01 -6.15 -2.54 -3.88 -4.33 -3.14 -8.67 -7.13 -8.18
Vacuole Periphery -2.65 1.37 -3.81 -3.27 -3.94 -3.60 -2.53 -2.66 -0.24 -1.74 -5.15 -2.95 -4.47 -3.28 -4.21