Standard name
Human Ortholog
Description Conserved NAD+ dependent histone deacetylase of the Sirtuin family; deacetylation targets are primarily nuclear proteins; required for telomere hypercluster formation in quiescent yeast cells; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and rDNA; negatively regulates initiation of DNA replication; functions as regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.08 0.07 0 – 0 0 0 0 – 0.2 0.21 0.05 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0.05 0 0.05 0 0 0 0.05 0 0
Endosome 0.1 0.12 0.08 0.17 0.18 0.2 0.18 – 0.15 0.07 0 0.06 – 0.28 0.12 0.25 0.21 0.24 0.18 0.13 0.13 0.28 0.34 0.44
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0
Mitochondria 0.2 0.05 0 0.05 0 0.12 0.14 – 0.3 0.21 0.54 0.3 – 0 0 0.16 0.09 0.11 0 0 0 0 0.05 0.09
Nucleus 0.31 0.3 0.42 0.23 0.21 0.2 0.3 – 0.21 0.23 0.15 0.19 – 0.1 0.23 0.23 0.2 0.27 0.11 0.15 0.11 0.05 0.05 0
Nuclear Periphery 0.24 0.3 0.29 0.12 0.24 0.18 0.1 – 0.13 0.16 0.13 0.16 – 0.12 0.08 0.28 0.23 0.17 0.34 0.34 0.34 0.18 0.13 0.07
Nucleolus 0.39 0.32 0.22 0.48 0.4 0.4 0.41 – 0.39 0.4 0.49 0.46 – 0.6 0.44 0.24 0.2 0.2 0.16 0.19 0.23 0.18 0.11 0.17
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0
SpindlePole 0 0 0.06 0.12 0.14 0.1 0.12 – 0.12 0.11 0.05 0.1 – 0.1 0.17 0.12 0.1 0.1 0.12 0.05 0.07 0.14 0.15 0.07
Vac/Vac Membrane 0.06 0.14 0.09 0 0.07 0 0 – 0 0 0 0 – 0.05 0.06 0.18 0.26 0.15 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 1 0 2 – 4 6 4 6 – 0 0 0 0 0 2 1 1 0 0 0
Bud Neck 0 0 0 0 1 0 3 – 0 1 0 3 – 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 8 – 7 9 17 9 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 2 13 22 22 17 – 12 21 16 10 – 8 10 5 13 14 0 1 0 0 0 0
Endoplasmic Reticulum 1 0 0 0 1 1 0 – 0 1 0 0 – 0 0 5 3 8 0 0 0 0 0 0
Endosome 27 18 11 31 50 66 81 – 47 34 12 25 – 11 6 24 23 41 57 28 29 3 12 16
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 – 0 0 1 2 0 2 1 0 0 1 1
Mitochondria 55 7 0 10 4 41 63 – 95 101 235 123 – 0 0 15 10 19 8 7 7 0 1 3
Nucleus 86 47 58 42 57 69 137 – 67 107 67 80 – 4 11 22 21 46 35 32 24 0 1 0
Nuclear Periphery 65 47 40 22 66 60 46 – 43 76 57 65 – 5 4 27 25 29 109 72 75 2 5 2
Nucleolus 108 49 31 87 110 136 189 – 126 190 217 192 – 24 21 23 21 34 50 41 50 2 4 6
Peroxisomes 1 1 0 3 1 3 7 – 1 0 0 0 – 0 0 1 0 0 2 0 1 0 0 0
SpindlePole 9 2 8 22 39 35 54 – 39 52 20 41 – 4 8 11 11 17 37 11 15 1 5 2
Vac/Vac Membrane 16 22 13 7 18 13 16 – 13 16 11 8 – 2 3 17 28 25 11 13 9 0 0 0
Unique Cell Count 276 155 139 182 275 338 456 320 470 439 414 40 48 95 107 168 323 213 224 13 38 38
Labelled Cell Count 372 198 164 237 372 446 623 454 614 656 562 58 63 151 157 233 323 213 224 13 38 38


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 8.3 7.9 7.6 7.5 6.5 6.2 – 5.6 5.7 5.0 5.3 – 8.1 7.3 7.9 8.0 7.5 – – –
Std Deviation (1e-4) 1.0 1.8 1.1 1.5 1.5 1.5 1.3 – 1.3 1.1 1.2 1.2 – 1.8 1.7 1.6 1.8 1.5 – – –
Intensity Change (Log2) – – – -0.05 -0.07 -0.28 -0.34 – -0.49 -0.47 -0.65 -0.56 – 0.04 -0.12 0.0 0.02 -0.08 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cytoplasm 2.4 2.7 2.3 0 – 0 1.6 0 0 – 0 0 0 3.5 2.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Endosome 2.4 2.8 3.1 2.8 – 2.0 -0.3 -2.7 -0.8 – 0 0 3.7 3.1 3.8
Golgi 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 4.3 4.6 – 7.2 6.0 11.2 7.3 – 0 0 4.8 0 4.1
Nucleus -3.6 -4.5 -4.8 -2.6 – -4.6 -4.4 -6.6 -5.3 – -3.7 -2.3 -2.9 -3.7 -2.6
Nuclear Periphery -3.8 -1.1 -2.7 -5.5 – -3.9 -3.3 -4.3 -3.4 – -2.1 -2.9 -0.1 -1.0 -2.4
Nucleolus 4.7 3.6 3.7 4.1 – 3.5 3.9 5.6 5.0 – 4.6 2.9 0.3 -0.5 -0.4
Peroxisomes 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
SpindlePole 1.9 2.6 1.6 2.1 – 2.1 1.8 -0.6 1.5 – 0 0 1.6 1.3 1.4
Vacuole -2.0 -1.0 -2.4 -2.8 – -2.3 -2.9 -3.5 -4.0 – 0 0 1.9 3.5 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5362 2.8258 2.3163 1.9202 2.0623 2.0628 2.083 2.4921 2.303 1.9217 1.6821 2.0754 1.6931 1.9363 1.9098 1.9239 1.4529 1.2913
Actin 0.0094 0.0001 0.0068 0.0005 0.0008 0.0005 0.0125 0.0003 0.0093 0.0016 0.0045 0.0005 0.0287 0.0003 0.043 0.0048 0.0004 0.0031
Bud 0.0003 0 0.0003 0 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0.0006 0.0001 0.0003 0.0002 0 0
Bud Neck 0.0014 0.0006 0.0005 0.0004 0.0005 0.0015 0.0005 0.0003 0.0005 0.0003 0.0009 0.0005 0.0013 0.0006 0.0008 0.0008 0.0006 0.0013
Bud Periphery 0.0007 0.0001 0.0009 0.0002 0.0003 0.0002 0.0004 0.0001 0.0004 0.0001 0.0002 0.0001 0.0011 0.0001 0.0006 0.0004 0.0001 0.0002
Bud Site 0.0018 0.0002 0.0009 0.0003 0.0002 0.0002 0.0006 0.0002 0.0114 0.0002 0.0003 0.0001 0.0021 0.0005 0.0022 0.0007 0.0002 0.0002
Cell Periphery 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0002 0 0.0001
Cytoplasm 0.0024 0.0003 0.0018 0.0002 0.0032 0.0002 0.0041 0.0001 0.0016 0.0018 0.0015 0.0003 0.0027 0.0006 0.0011 0.0241 0.0021 0.0003
Cytoplasmic Foci 0.0121 0.006 0.0166 0.0072 0.0081 0.0056 0.0161 0.0037 0.021 0.0147 0.0217 0.004 0.0302 0.0083 0.0176 0.0192 0.0053 0.0037
Eisosomes 0.0002 0 0.0002 0 0.0001 0 0.0002 0 0.0002 0.0001 0.0003 0 0.0002 0 0.0011 0.0001 0 0.0001
Endoplasmic Reticulum 0.0013 0.0002 0.0022 0.0002 0.0014 0.0002 0.0012 0.0002 0.0011 0.0007 0.0009 0.0006 0.0041 0.0002 0.0009 0.0031 0.0005 0.0004
Endosome 0.0152 0.0013 0.0202 0.002 0.0169 0.004 0.0134 0.0012 0.0184 0.0048 0.0164 0.0079 0.0306 0.0021 0.0057 0.0128 0.0022 0.0019
Golgi 0.0055 0.0016 0.0063 0.0024 0.0029 0.003 0.0074 0.002 0.0103 0.0051 0.009 0.0039 0.017 0.0022 0.0062 0.009 0.0022 0.0025
Lipid Particles 0.083 0.0752 0.0907 0.0605 0.0634 0.0743 0.1372 0.0538 0.0877 0.1221 0.0921 0.0643 0.1041 0.0554 0.0839 0.1003 0.0524 0.0458
Mitochondria 0.0095 0.0002 0.0176 0.0003 0.003 0.0007 0.0028 0.0002 0.0071 0.0203 0.0081 0.0013 0.0086 0.0003 0.0144 0.0038 0.0004 0.0024
None 0.0038 0.0002 0.0016 0.0004 0.0012 0.0002 0.0053 0.0003 0.0031 0.0011 0.0058 0.0003 0.0016 0.0003 0.0018 0.0116 0.0012 0.0002
Nuclear Periphery 0.3672 0.2324 0.3092 0.2858 0.3908 0.2435 0.3751 0.3298 0.3148 0.2449 0.2165 0.2685 0.2833 0.1492 0.2232 0.1151 0.2526 0.1841
Nucleolus 0.1354 0.2669 0.1592 0.1568 0.1897 0.2308 0.1324 0.2275 0.1652 0.1639 0.1701 0.2115 0.1637 0.3444 0.2557 0.3301 0.1704 0.3068
Nucleus 0.044 0.0647 0.0762 0.0531 0.0791 0.0554 0.0409 0.0413 0.0323 0.0232 0.0474 0.0539 0.0446 0.1228 0.0875 0.0801 0.1083 0.0982
Peroxisomes 0.0074 0.0006 0.0035 0.0012 0.0018 0.0019 0.0097 0.0008 0.013 0.0184 0.0182 0.0007 0.0195 0.0013 0.0114 0.0106 0.0007 0.001
Punctate Nuclear 0.2925 0.3471 0.272 0.4262 0.2318 0.3754 0.2335 0.3349 0.2972 0.3704 0.384 0.3785 0.2492 0.309 0.237 0.2695 0.3979 0.3454
Vacuole 0.0022 0.0005 0.0067 0.0003 0.0021 0.0007 0.0013 0.0002 0.0013 0.0007 0.0007 0.0004 0.0022 0.0007 0.0013 0.0012 0.0005 0.0005
Vacuole Periphery 0.0045 0.0017 0.0063 0.0017 0.0027 0.0016 0.0051 0.0032 0.0038 0.0051 0.0014 0.0024 0.0046 0.0017 0.0041 0.0025 0.0018 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.6789 2.336 4.3335 8.7158 11.8582 17.629 6.4953 4.9817 5.6169 12.191
Translational Efficiency 1.1143 2.5116 1.2559 0.6084 0.7719 0.8972 0.781 0.788 0.9721 0.9785

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Conserved NAD+ dependent histone deacetylase of the Sirtuin family; deacetylation targets are primarily nuclear proteins; required for telomere hypercluster formation in quiescent yeast cells; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and rDNA; negatively regulates initiation of DNA replication; functions as regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy
Localization
Cell Percentages nuclear periphery (63%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sir2

Sir2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sir2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available