Standard name
Description Conserved NAD+ dependent histone deacetylase of the Sirtuin family; deacetylation targets are primarily nuclear proteins; required for telomere hypercluster formation in quiescent yeast cells; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and rDNA; negatively regulates initiation of DNA replication; functions as regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy
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ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.08 0.07 0 – 0 0 0 0 – 0.2 0.21 0.05 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0.05 0 0.05 0 0 0 0.05 0 0
Endosome 0.1 0.12 0.08 0.17 0.18 0.2 0.18 – 0.15 0.07 0 0.06 – 0.28 0.12 0.25 0.21 0.24 0.18 0.13 0.13 0.28 0.34 0.44
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0
Mitochondria 0.2 0.05 0 0.05 0 0.12 0.14 – 0.3 0.21 0.54 0.3 – 0 0 0.16 0.09 0.11 0 0 0 0 0.05 0.09
Nucleus 0.31 0.3 0.42 0.23 0.21 0.2 0.3 – 0.21 0.23 0.15 0.19 – 0.1 0.23 0.23 0.2 0.27 0.11 0.15 0.11 0.05 0.05 0
Nuclear Periphery 0.24 0.3 0.29 0.12 0.24 0.18 0.1 – 0.13 0.16 0.13 0.16 – 0.12 0.08 0.28 0.23 0.17 0.34 0.34 0.34 0.18 0.13 0.07
Nucleolus 0.39 0.32 0.22 0.48 0.4 0.4 0.41 – 0.39 0.4 0.49 0.46 – 0.6 0.44 0.24 0.2 0.2 0.16 0.19 0.23 0.18 0.11 0.17
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0
SpindlePole 0 0 0.06 0.12 0.14 0.1 0.12 – 0.12 0.11 0.05 0.1 – 0.1 0.17 0.12 0.1 0.1 0.12 0.05 0.07 0.14 0.15 0.07
Vac/Vac Membrane 0.06 0.14 0.09 0 0.07 0 0 – 0 0 0 0 – 0.05 0.06 0.18 0.26 0.15 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 0 0 1 0 2 – 4 6 4 6 – 0 0 0 0 0 2 1 1 0 0 0
Bud Neck 0 0 0 0 1 0 3 – 0 1 0 3 – 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 8 – 7 9 17 9 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 2 13 22 22 17 – 12 21 16 10 – 8 10 5 13 14 0 1 0 0 0 0
Endoplasmic Reticulum 1 0 0 0 1 1 0 – 0 1 0 0 – 0 0 5 3 8 0 0 0 0 0 0
Endosome 27 18 11 31 50 66 81 – 47 34 12 25 – 11 6 24 23 41 57 28 29 3 12 16
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 – 0 0 1 2 0 2 1 0 0 1 1
Mitochondria 55 7 0 10 4 41 63 – 95 101 235 123 – 0 0 15 10 19 8 7 7 0 1 3
Nucleus 86 47 58 42 57 69 137 – 67 107 67 80 – 4 11 22 21 46 35 32 24 0 1 0
Nuclear Periphery 65 47 40 22 66 60 46 – 43 76 57 65 – 5 4 27 25 29 109 72 75 2 5 2
Nucleolus 108 49 31 87 110 136 189 – 126 190 217 192 – 24 21 23 21 34 50 41 50 2 4 6
Peroxisomes 1 1 0 3 1 3 7 – 1 0 0 0 – 0 0 1 0 0 2 0 1 0 0 0
SpindlePole 9 2 8 22 39 35 54 – 39 52 20 41 – 4 8 11 11 17 37 11 15 1 5 2
Vac/Vac Membrane 16 22 13 7 18 13 16 – 13 16 11 8 – 2 3 17 28 25 11 13 9 0 0 0
Unique Cell Count 276 155 139 182 275 338 456 320 470 439 414 40 48 95 107 168 323 213 224 13 38 38
Labelled Cell Count 372 198 164 237 372 446 623 454 614 656 562 58 63 151 157 233 323 213 224 13 38 38


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 8.3 7.9 7.6 7.5 6.5 6.2 – 5.6 5.7 5.0 5.3 – 8.1 7.3 7.9 8.0 7.5 – – –
Std Deviation (1e-4) 1.0 1.8 1.1 1.5 1.5 1.5 1.3 – 1.3 1.1 1.2 1.2 – 1.8 1.7 1.6 1.8 1.5 – – –
Intensity Change (Log2) – – – -0.05 -0.07 -0.28 -0.34 – -0.49 -0.47 -0.65 -0.56 – 0.04 -0.12 0.0 0.02 -0.08 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Cytoplasm 2.4 2.7 2.3 0 – 0 1.6 0 0 – 0 0 0 3.5 2.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Endosome 2.4 2.8 3.1 2.8 – 2.0 -0.3 -2.7 -0.8 – 0 0 3.7 3.1 3.8
Golgi 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 4.3 4.6 – 7.2 6.0 11.2 7.3 – 0 0 4.8 0 4.1
Nucleus -3.6 -4.5 -4.8 -2.6 – -4.6 -4.4 -6.6 -5.3 – -3.7 -2.3 -2.9 -3.7 -2.6
Nuclear Periphery -3.8 -1.1 -2.7 -5.5 – -3.9 -3.3 -4.3 -3.4 – -2.1 -2.9 -0.1 -1.0 -2.4
Nucleolus 4.7 3.6 3.7 4.1 – 3.5 3.9 5.6 5.0 – 4.6 2.9 0.3 -0.5 -0.4
Peroxisomes 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0
SpindlePole 1.9 2.6 1.6 2.1 – 2.1 1.8 -0.6 1.5 – 0 0 1.6 1.3 1.4
Vacuole -2.0 -1.0 -2.4 -2.8 – -2.3 -2.9 -3.5 -4.0 – 0 0 1.9 3.5 1.5