Standard name
Description Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.11 0 0.11 0.13 0.19 0.14 0.2 0.12 0.19 0.11 0.14 0.12 0.23 0.25 0.26 0.3 0 0.05 0 0 0.06 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.22 0.22 0.46 0.53 0.62 0.67 0.8 0.76 0.84 0.77 0.84 0.75 0.08 0.08 0 0.07 0.07 0.05 0 0 0.05 0.12 0.11
Nucleus 0.56 0.47 0.5 0.58 0.43 0.32 0.28 0.35 0.31 0.25 0.37 0.35 0.75 0.54 0.49 0.3 0.45 0.48 0.55 0.55 0.51 0.35 0.17
Nuclear Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.25 0.47 0.12 0.09 0.08 0.13 0.1 0 0.05 0 0.07 0.07 0.09 0.21 0.29 0.4 0.27 0.32 0.31 0.32 0.34 0.36 0.46
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud 0 0 0 1 1 1 1 2 0 1 0 0 0 0 0 0 0 1 0 0 0 5 8
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 1 2 0 1 2 0 2 0 0 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 9 21 0 10 7 12 20 22 11 17 12 16 30 77 100 26 26 8 11 1 7 18 17
Endoplasmic Reticulum 0 1 1 0 0 0 0 0 0 0 0 0 3 3 2 6 2 4 0 0 2 3 6
Endosome 12 1 0 0 0 0 0 0 0 0 0 0 0 2 6 4 2 2 2 2 3 3 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 1 0 0 1
Mitochondria 36 43 11 47 33 42 114 84 74 68 89 85 20 28 18 7 6 11 8 0 9 34 35
Nucleus 92 90 12 52 23 20 40 39 27 22 39 40 187 184 196 30 39 102 122 33 103 102 57
Nuclear Periphery 0 2 0 0 1 0 2 0 4 1 2 2 0 1 1 0 0 1 1 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 2 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 1 0 0 1 1 2 1 1 1 0 5 2 0 2 3 14
Vac/Vac Membrane 41 90 3 8 4 8 15 4 4 3 7 8 22 71 115 40 23 68 70 19 69 104 153
Unique Cell Count 165 192 24 89 53 63 143 111 88 88 106 113 248 341 403 99 86 215 225 61 203 290 330
Labelled Cell Count 193 248 28 119 69 84 194 154 121 115 150 156 265 368 439 114 99 215 225 61 203 290 330


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.2 3.8 3.8 3.9 3.7 3.2 3.7 3.5 3.3 3.4 3.4 5.0 5.1 4.7 4.4 5.4 5.4 4.9 5.0 5.5
Std Deviation (1e-4) 1.0 0.8 0.9 0.7 1.1 1.3 1.3 1.5 1.1 1.4 1.3 1.8 1.0 4.9 1.2 0.8 1.7 0.8 1.4 1.7 2.0
Intensity Change (Log2) -0.01 0.04 -0.05 -0.25 -0.07 -0.15 -0.21 -0.17 -0.17 0.37 0.41 0.31 0.21 0.5 0.49 0.34 0.38 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 2.6 1.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pus9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available