Standard name
Human Ortholog
Description Subunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0.05 0 0 0.05 0.07 0 0.11 0.13 0.18 0.08 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.14 0.43 0.43 0.37 0.67 0.7 0.54 0.69 0.79 0.72 0.8 0.05 0 0 0 0.07 0.1 0 0 0 0 0 0
Nucleus 0.96 0.93 0.89 0.88 0.87 0.77 0.69 0.78 0.72 0.71 0.72 0.68 0.88 0.83 0.78 0.87 0.82 0.81 0.88 0.88 0.87 0.83 0.77 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0.05 0.07 0.06 0.1 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 3 3 7 4 0 1 0 0 0 0 0 0 0 4 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 12 8 12 8 6 12 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 3 4 8 14 11 23 24 16 18 13 5 19 50 65 7 3 1 0 1 1 1 1 1
Endoplasmic Reticulum 0 1 0 0 1 1 1 0 0 0 0 0 0 2 0 3 1 1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 1 0 1 2 0 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 1 2
Mitochondria 27 43 84 79 106 220 334 319 291 307 141 127 9 8 6 3 3 2 0 3 3 0 4 4
Nucleus 149 285 175 161 249 252 329 461 302 273 141 107 150 312 283 72 33 17 138 273 169 145 155 124
Nuclear Periphery 1 1 0 4 3 7 9 7 6 6 1 4 1 1 2 0 0 0 0 0 0 2 0 2
Nucleolus 9 7 1 0 2 4 7 17 14 5 7 5 3 6 1 0 0 0 6 3 0 3 10 8
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 2 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 0 0 1 6 5 1 0 0 1 0 3 5 1 0 1 0 2 1 1 1 0
Vac/Vac Membrane 1 9 1 2 6 2 9 3 2 1 0 4 1 7 9 5 0 1 6 16 14 11 20 35
Unique Cell Count 156 305 196 184 285 327 477 588 421 387 195 158 171 377 364 83 40 21 158 312 195 175 202 189
Labelled Cell Count 191 351 266 255 381 510 729 851 647 620 315 259 183 394 378 91 41 23 158 312 195 175 202 189


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.1 4.2 4.0 4.2 3.7 3.2 3.7 3.5 3.2 3.5 3.2 5.7 5.8 5.7 6.8 7.2 8.1 5.5 5.7 6.2
Std Deviation (1e-4) 0.8 0.9 0.7 1.0 1.2 1.0 1.2 1.0 0.9 1.4 1.4 1.0 1.3 1.6 1.4 2.0 1.6 1.6 1.4 1.3 1.1
Intensity Change (Log2) -0.07 -0.03 -0.21 -0.39 -0.19 -0.28 -0.4 -0.28 -0.4 0.41 0.46 0.42 0.68 0.77 0.93 0.38 0.43 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.6 0.9 1.7 1.3 1.1 1.6 2.2 0 3.6 4.4 5.4 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -8.3 -12.6 -12.7 -6.5 -4.2 -3.0
Nucleus -0.5 -0.6 -3.5 -5.5 -3.4 -4.8 -5.1 -4.3 -5.0 -0.5 -2.1 -3.4 -0.6 -1.2 -1.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9694 4.5751 4.478 4.334 4.5563 4.3711 2.7288 2.9741 2.4119 2.0286 1.9265 2.2314 2.5726 3.2757 3.3368 3.6512 3.3267 3.4674
Actin 0.0621 0.0006 0.0066 0.001 0.0003 0.0037 0.0303 0 0.019 0 0.0029 0.0015 0 0 0.045 0.0001 0.006 0.0047
Bud 0.0012 0.0002 0.0003 0.0006 0.0001 0.0002 0.0008 0.0001 0.0006 0 0.0017 0 0 0 0.0001 0.0002 0.0049 0.0003
Bud Neck 0.0251 0.0023 0.0008 0.001 0.0006 0.0007 0.0018 0.0002 0.0014 0 0.0004 0.0005 0 0.0001 0.0001 0.0003 0.0008 0.0006
Bud Periphery 0.0035 0.0004 0.001 0.002 0.0001 0.0005 0.0014 0.0002 0.0009 0 0.0036 0 0 0 0.0001 0.0006 0.0059 0.0012
Bud Site 0.0108 0.0093 0.0013 0.0005 0.0005 0.0004 0.0055 0.0024 0.0063 0 0.0011 0.0001 0 0 0.0002 0.0001 0.0065 0.0003
Cell Periphery 0.0015 0.0007 0.0003 0.0022 0 0.0001 0.0006 0.0001 0.0003 0 0.0002 0 0 0 0 0.0001 0.0002 0.0004
Cytoplasm 0.0287 0.0001 0.0004 0.0029 0.0005 0.0006 0.008 0.0006 0.001 0.0019 0.0015 0.0005 0 0.0004 0.0002 0.0002 0.0148 0.001
Cytoplasmic Foci 0.0076 0.0009 0.005 0.0004 0.0178 0.0021 0.0429 0.0027 0.0261 0 0.0078 0.0067 0 0 0.0022 0 0.0334 0.0002
Eisosomes 0.0004 0.0001 0.0002 0 0 0 0.0004 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0088 0.0001 0.0013 0.0018 0.0001 0.0001 0.004 0 0.0004 0 0.0014 0.0001 0 0 0 0.0001 0.0039 0.0042
Endosome 0.0311 0.0011 0.0109 0.0117 0.0071 0.0004 0.0682 0.0001 0.0038 0 0.0141 0.008 0 0 0.001 0.0001 0.0891 0.0015
Golgi 0.0125 0.0006 0.0058 0.0074 0.0022 0.0007 0.0279 0.0001 0.004 0 0.0056 0.0031 0 0 0.0096 0 0.0135 0.0014
Lipid Particles 0.0197 0.0034 0.0176 0.001 0.0099 0.001 0.0406 0.0022 0.0168 0 0.0332 0.0026 0 0 0.0004 0 0.0061 0.0001
Mitochondria 0.0167 0.005 0.0237 0.039 0.0009 0.0022 0.0155 0.0004 0.0073 0 0.0136 0.0024 0.0001 0.0003 0.0017 0.0013 0.0407 0.0024
None 0.0015 0.0001 0.0008 0.0006 0.0004 0.0001 0.0251 0.0007 0.0004 0.001 0.0003 0.0001 0 0.0001 0 0.0001 0.0028 0.0001
Nuclear Periphery 0.0405 0.0011 0.0094 0.009 0.0011 0.0014 0.0111 0.0025 0.0038 0.0024 0.0019 0.0002 0.0001 0.0021 0.0016 0.002 0.02 0.0006
Nucleolus 0.0096 0.0126 0.008 0.0232 0.0188 0.0193 0.0225 0.0119 0.0047 0.004 0.0264 0.0155 0.0054 0.0076 0.0035 0.0169 0.0119 0.012
Nucleus 0.6942 0.9566 0.8883 0.8628 0.9231 0.9614 0.6386 0.9721 0.8802 0.9835 0.8385 0.9511 0.9943 0.9888 0.9319 0.9758 0.6983 0.9681
Peroxisomes 0.0048 0.0019 0.0114 0.0002 0.0089 0.0024 0.0217 0.0016 0.0202 0 0.0118 0.0064 0 0 0.0023 0 0.0133 0
Punctate Nuclear 0.0051 0.0012 0.0032 0.0002 0.0072 0.0023 0.0242 0.0019 0.001 0.007 0.0301 0.0011 0 0.0003 0.0002 0.0001 0.0111 0.0002
Vacuole 0.0096 0.0015 0.002 0.021 0.0003 0.0003 0.0068 0.0002 0.0013 0 0.0026 0.0001 0 0.0001 0 0.001 0.0099 0.0003
Vacuole Periphery 0.0051 0.0002 0.0015 0.0112 0.0001 0.0001 0.0024 0.0001 0.0004 0 0.0013 0.0001 0 0.0001 0 0.0009 0.0067 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4289 8.0495 14.703 10.3612 10.3277 9.6918 11.7387 10.5638 12.021 12.005
Translational Efficiency 1.2452 1.5952 0.7229 1.026 1.1683 1.1424 0.9911 0.9901 0.8795 1.1834

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1826 1201 561 1794 1787 1997 176 101 3613 3198 737 1895

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 705.42 757.86 1023.86 989.53 761.39 807.52 1026.80 1065.99 733.10 788.87 1024.56 993.61
Standard Deviation 80.72 119.75 122.57 138.12 92.07 111.95 126.72 131.38 90.93 117.43 123.58 138.84
Intensity Change Log 2 0.103449 0.537464 0.488261 0.084862 0.431448 0.485486 0.093831 0.483406 0.486821

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000147 0.000488 0.000882 0.000945 0.000185 0.000262 0.001273 0.001527 0.000166 0.000347 0.000975 0.000976
Bud Neck 0.001790 0.005547 0.010990 0.028242 0.002281 0.006471 0.004623 0.012133 0.002033 0.006124 0.009469 0.027383
Bud Site 0.000598 0.001830 0.001206 0.007230 0.000825 0.001388 0.001061 0.002585 0.000711 0.001554 0.001171 0.006983
Cell Periphery 0.000081 0.000149 0.000047 0.000127 0.000266 0.000085 0.000117 0.000128 0.000173 0.000109 0.000064 0.000127
Cytoplasm 0.015089 0.013753 0.001385 0.007293 0.004517 0.005658 0.006012 0.005048 0.009860 0.008698 0.002490 0.007173
Cytoplasmic Foci 0.001617 0.001020 0.000043 0.000648 0.000993 0.002088 0.000080 0.005993 0.001308 0.001687 0.000052 0.000933
Eisosomes 0.000012 0.000019 0.000028 0.000023 0.000014 0.000013 0.000046 0.000041 0.000013 0.000015 0.000033 0.000024
Endoplasmic Reticulum 0.001347 0.005118 0.005590 0.007762 0.002670 0.004258 0.008463 0.013779 0.002002 0.004581 0.006276 0.008083
Endosome 0.000519 0.000405 0.000225 0.002039 0.000246 0.000933 0.000279 0.010145 0.000384 0.000735 0.000238 0.002471
Golgi 0.000368 0.000240 0.000080 0.001344 0.000405 0.000967 0.000089 0.002759 0.000386 0.000694 0.000082 0.001419
Lipid Particles 0.000811 0.000434 0.000149 0.000168 0.000457 0.000423 0.000235 0.000635 0.000636 0.000427 0.000169 0.000193
Mitochondria 0.002548 0.001945 0.001047 0.005002 0.001851 0.004490 0.001143 0.001770 0.002203 0.003535 0.001070 0.004829
Mitotic Spindle 0.001413 0.003667 0.000748 0.026332 0.001591 0.005105 0.000558 0.001535 0.001501 0.004565 0.000703 0.025010
None 0.010933 0.014390 0.001777 0.004957 0.003852 0.003388 0.014080 0.005982 0.007431 0.007520 0.004715 0.005012
Nuclear Periphery 0.000568 0.000429 0.000372 0.003545 0.000580 0.001342 0.000580 0.001123 0.000574 0.000999 0.000422 0.003416
Nuclear Periphery Foci 0.000700 0.000388 0.000183 0.000634 0.000174 0.000572 0.000148 0.000445 0.000440 0.000503 0.000174 0.000624
Nucleolus 0.011145 0.014790 0.008601 0.004297 0.008272 0.008437 0.008226 0.006536 0.009724 0.010823 0.008512 0.004416
Nucleus 0.948552* 0.933658* 0.964490* 0.891592* 0.969184* 0.950910* 0.948753* 0.920926* 0.958757* 0.944431* 0.960732* 0.893155*
Peroxisomes 0.001058 0.000672 0.000250 0.000200 0.000816 0.000615 0.000356 0.000206 0.000939 0.000636 0.000275 0.000200
Vacuole 0.000572 0.000798 0.001793 0.006739 0.000652 0.001848 0.003750 0.006060 0.000612 0.001454 0.002260 0.006702
Vacuole Periphery 0.000130 0.000258 0.000113 0.000881 0.000168 0.000746 0.000128 0.000642 0.000149 0.000563 0.000117 0.000868

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.58 -9.56 -14.01 -10.69 -0.97 -0.85 -9.37 -8.38 -7.92 -1.16 -3.20 -12.75 -16.33 -12.86 -0.04
Bud Neck -6.26 -10.49 -20.59 -17.12 -10.42 -4.92 -6.44 -7.47 -5.41 -5.11 -7.30 -11.29 -20.92 -17.24 -12.10
Bud Site -2.43 -3.07 -6.96 -4.65 -5.45 -1.15 -2.63 -3.95 -2.97 -2.36 -2.50 -3.35 -6.94 -5.58 -5.57
Cell Periphery -2.54 2.60 -3.04 1.20 -5.98 0.98 0.82 0.78 -1.47 -0.26 0.69 1.14 0.60 -0.54 -4.72
Cytoplasm 0.57 8.94 4.23 2.61 -7.42 -1.18 -0.05 0.33 1.27 0.24 0.85 7.39 1.66 0.64 -6.13
Cytoplasmic Foci 1.40 4.20 3.53 2.51 -2.75 -1.58 4.15 -0.56 -0.13 -1.00 -0.49 5.72 3.70 3.24 -2.38
Eisosomes -4.38 -9.69 -14.57 -7.28 0.22 0.47 -9.08 -6.51 -6.77 0.50 -2.05 -12.42 -14.69 -14.03 2.74
Endoplasmic Reticulum -13.12 -12.98 -21.80 -8.85 -5.27 -3.98 -9.41 -6.47 -5.49 -1.92 -9.68 -14.60 -20.79 -11.13 -3.92
Endosome 0.74 1.56 -2.36 -2.96 -3.41 -3.35 -2.32 -1.10 -0.93 -1.05 -2.38 0.64 -3.05 -1.94 -3.26
Golgi 0.87 2.11 -1.04 -1.72 -2.49 -1.19 1.09 -0.71 -0.21 -1.01 -1.06 1.96 -1.08 0.03 -2.65
Lipid Particles 1.83 3.13 2.67 1.55 -2.29 0.43 0.99 -0.06 -0.37 -0.72 1.84 3.60 3.03 2.02 -1.70
Mitochondria 0.70 1.44 -2.78 -4.91 -6.77 -2.54 1.03 -0.69 2.39 -3.24 -1.77 1.63 -4.27 -1.88 -7.45
Mitotic Spindle -1.80 0.30 -8.14 -6.61 -8.21 -2.58 0.76 -0.99 0.25 -1.25 -3.23 0.61 -8.18 -6.74 -8.33
None -1.56 6.71 4.90 5.65 -4.32 0.62 -1.37 -0.45 -0.76 1.09 -0.05 3.29 3.85 4.00 -0.70
Nuclear Periphery 1.71 0.27 -9.80 -10.17 -9.89 -3.64 -2.51 -4.17 -1.54 -2.92 -3.20 -0.88 -9.99 -8.64 -9.83
Nuclear Periphery Foci 1.09 1.77 0.27 -2.13 -4.45 -1.56 -1.07 -1.77 0.15 0.07 -0.13 1.07 -1.00 -0.89 -3.04
Nucleolus -2.06 1.35 4.77 6.99 3.17 0.14 0.45 0.09 0.05 -0.11 -0.81 0.85 4.73 6.07 3.18
Nucleus 2.68 -2.18 11.47 8.12 13.79 4.24 3.67 4.20 2.83 1.83 4.11 1.33 15.09 11.70 13.21
Peroxisomes 1.14 2.26 2.52 2.59 1.89 0.97 2.05 2.36 2.77 1.50 1.54 3.07 3.55 4.22 2.53
Vacuole -1.07 -5.27 -10.07 -9.87 -8.00 -3.80 -3.46 -2.32 -2.07 -0.28 -4.00 -5.02 -10.34 -9.33 -5.23
Vacuole Periphery -1.89 -0.29 -3.53 -1.97 -3.43 -3.85 0.49 -0.97 1.55 -1.09 -4.31 0.26 -3.43 0.54 -3.54