Standard name | |
---|---|
Human Ortholog | |
Description | Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 14 | 51 | 75 | 47 | 30 | 71 | 35 | 10 | 5 | 7 | 3 | 9 | 36 | 5 | 1 | 40 | 14 | 19 | 11 | 27 | 35 | 25 | 40 | 24 |
Bud | 3 | 19 | 20 | 14 | 22 | 35 | 24 | 22 | 26 | 23 | 16 | 5 | 13 | 5 | 1 | 7 | 5 | 7 | 14 | 46 | 52 | 37 | 12 | 9 |
Bud Neck | 4 | 16 | 36 | 22 | 28 | 24 | 33 | 21 | 18 | 10 | 10 | 15 | 2 | 1 | 2 | 10 | 9 | 0 | 34 | 85 | 136 | 54 | 22 | 12 |
Bud Site | 7 | 35 | 57 | 24 | 27 | 67 | 19 | 4 | 15 | 5 | 4 | 5 | 23 | 1 | 1 | 24 | 8 | 14 | – | – | – | – | – | – |
Cell Periphery | 1 | 8 | 6 | 10 | 1 | 7 | 6 | 4 | 3 | 2 | 1 | 2 | 1 | 4 | 4 | 4 | 8 | 1 | 1 | 7 | 14 | 9 | 16 | 13 |
Cytoplasm | 45 | 113 | 175 | 174 | 172 | 132 | 233 | 275 | 294 | 206 | 174 | 198 | 162 | 247 | 207 | 130 | 95 | 75 | 24 | 53 | 59 | 38 | 81 | 138 |
Endoplasmic Reticulum | 1 | 4 | 0 | 1 | 5 | 5 | 12 | 5 | 1 | 2 | 1 | 4 | 2 | 4 | 2 | 10 | 5 | 6 | 1 | 1 | 2 | 4 | 5 | 7 |
Endosome | 1 | 6 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 6 | 4 | 3 | 4 | 8 | 5 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 1 |
Mitochondria | 7 | 5 | 0 | 2 | 6 | 24 | 42 | 37 | 102 | 108 | 104 | 56 | 3 | 2 | 2 | 9 | 5 | 8 | 3 | 13 | 28 | 13 | 29 | 24 |
Nucleus | 0 | 2 | 5 | 4 | 8 | 6 | 2 | 7 | 1 | 2 | 0 | 2 | 1 | 0 | 0 | 4 | 2 | 6 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 6 | 1 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 6 | 8 | 3 | 4 | 0 | 14 | 0 | 0 | 3 | 0 | 1 | 4 | 12 | 3 | 1 | 3 | 4 | 4 | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 1 | 19 | 77 | 32 | 50 | 60 | 29 | 27 | 24 | 15 | 7 | 14 | 3 | 3 | 0 | 33 | 23 | 17 | 2 | 2 | 9 | 3 | 0 | 2 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 5 | 2 | 1 | 3 | 2 | 0 | 0 | 0 | 4 | 3 | 2 | 3 | 10 | 11 | 4 | 10 | 11 |
Unique Cell Count | 73 | 209 | 321 | 258 | 276 | 303 | 355 | 371 | 402 | 314 | 261 | 277 | 219 | 261 | 214 | 220 | 151 | 125 | 104 | 257 | 368 | 201 | 225 | 257 |
Labelled Cell Count | 90 | 288 | 455 | 334 | 353 | 449 | 436 | 424 | 496 | 384 | 325 | 317 | 259 | 276 | 221 | 284 | 189 | 165 | 104 | 257 | 368 | 201 | 225 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 5.2 | 5.3 | 5.0 | 5.3 | 4.3 | 4.1 | 4.4 | 3.4 | 3.2 | 3.0 | 3.6 | 4.5 | 4.4 | 4.3 | 7.0 | 7.2 | 6.5 | 4.5 | 4.2 | 4.5 |
Std Deviation (1e-4) | 0.9 | 1.0 | 1.2 | 1.4 | 1.7 | 1.2 | 0.9 | 1.1 | 0.7 | 1.0 | 0.7 | 0.7 | 1.0 | 1.4 | 1.6 | 1.7 | 1.8 | 1.9 | 1.4 | 1.5 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.01 | -0.29 | -0.35 | -0.25 | -0.64 | -0.73 | -0.81 | -0.57 | -0.23 | -0.26 | -0.29 | 0.41 | 0.46 | 0.3 | -0.23 | -0.32 | -0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.5 | -4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.4 | -3.7 | -1.9 |
Bud | -0.4 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.6 | -1.3 | -0.3 |
Bud Neck | -1.1 | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.7 | -1.8 | -3.9 |
Bud Site | -2.9 | -2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.2 | -3.7 | -1.7 |
Cell Periphery | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.2 | 1.9 | -2.7 | 3.0 | 5.4 | 5.2 | 2.9 | 3.0 | 4.3 | 4.6 | 10.8 | 10.6 | 1.1 | 1.7 | 1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 1.1 | 0.4 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -3.5 | -1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.6 | -2.2 | -2.4 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.9075 | -0.995 | -0.9477 | -0.9657 | -2.6271 | -0.1279 | 1.6086 | -0.0125 | -0.1904 | -0.2231 | -0.5246 | 0.5089 | 0.3991 | -0.7265 | -0.9677 | -0.6991 | -1.1267 | 0.0363 |
Actin | 0.1106 | 0.0105 | 0.0068 | 0.0441 | 0.0037 | 0.0568 | 0.0846 | 0.0139 | 0.047 | 0.0545 | 0.0343 | 0.0288 | 0.0889 | 0.0101 | 0.0074 | 0.0003 | 0.008 | 0.0357 |
Bud | 0.0018 | 0.0057 | 0.002 | 0.0083 | 0.0003 | 0.0004 | 0.0035 | 0.0032 | 0.0028 | 0.0032 | 0.0027 | 0.0016 | 0.0014 | 0.0051 | 0.0019 | 0.0005 | 0.001 | 0.0007 |
Bud Neck | 0.1072 | 0.0087 | 0.038 | 0.1544 | 0.0882 | 0.7763 | 0.1239 | 0.0093 | 0.2073 | 0.4511 | 0.1985 | 0.6796 | 0.1208 | 0.0117 | 0.1082 | 0.2131 | 0.3217 | 0.6934 |
Bud Periphery | 0.0028 | 0.0104 | 0.0059 | 0.0146 | 0.001 | 0.0012 | 0.0081 | 0.0063 | 0.0058 | 0.0227 | 0.017 | 0.0056 | 0.0022 | 0.0165 | 0.0029 | 0.0011 | 0.0027 | 0.0017 |
Bud Site | 0.1202 | 0.1674 | 0.0752 | 0.0936 | 0.0018 | 0.0022 | 0.1266 | 0.264 | 0.1572 | 0.0191 | 0.0031 | 0.0066 | 0.1183 | 0.2539 | 0.0811 | 0.0092 | 0.0069 | 0.0028 |
Cell Periphery | 0.022 | 0.0172 | 0.0035 | 0.0069 | 0.0016 | 0.0013 | 0.0236 | 0.0098 | 0.008 | 0.0019 | 0.0016 | 0.0046 | 0.0146 | 0.0174 | 0.0036 | 0.0006 | 0.0012 | 0.0012 |
Cytoplasm | 0.0887 | 0.1805 | 0.0545 | 0.0115 | 0.046 | 0.0366 | 0.1912 | 0.1909 | 0.1807 | 0.0706 | 0.1156 | 0.1247 | 0.082 | 0.1637 | 0.0966 | 0.11 | 0.0464 | 0.0907 |
Cytoplasmic Foci | 0.0489 | 0.0091 | 0.035 | 0.0596 | 0.1903 | 0.016 | 0.0377 | 0.0109 | 0.0186 | 0.0242 | 0.0616 | 0.0051 | 0.0565 | 0.0072 | 0.0313 | 0.0117 | 0.0146 | 0.0154 |
Eisosomes | 0.0025 | 0.0013 | 0.0022 | 0.0011 | 0.0013 | 0.0005 | 0.0013 | 0.001 | 0.0006 | 0.0003 | 0.0011 | 0.0004 | 0.0018 | 0.0011 | 0.0012 | 0.0001 | 0.0005 | 0.0004 |
Endoplasmic Reticulum | 0.0153 | 0.0033 | 0.0009 | 0.001 | 0.0007 | 0.0121 | 0.0099 | 0.0067 | 0.0022 | 0.0013 | 0.0036 | 0.0016 | 0.0047 | 0.003 | 0.0044 | 0.0012 | 0.0011 | 0.0009 |
Endosome | 0.0683 | 0.0016 | 0.0033 | 0.0018 | 0.0046 | 0.009 | 0.0269 | 0.0047 | 0.0061 | 0.0069 | 0.0496 | 0.0015 | 0.0277 | 0.0009 | 0.0052 | 0.0553 | 0.0078 | 0.0121 |
Golgi | 0.0108 | 0.0005 | 0.0018 | 0.005 | 0.0022 | 0.0078 | 0.0122 | 0.0011 | 0.006 | 0.0114 | 0.0085 | 0.001 | 0.0045 | 0.0006 | 0.0028 | 0.0092 | 0.0076 | 0.0131 |
Lipid Particles | 0.05 | 0.0016 | 0.002 | 0.0034 | 0.0022 | 0.0099 | 0.0483 | 0.0038 | 0.0054 | 0.0163 | 0.008 | 0.0004 | 0.008 | 0.0012 | 0.003 | 0.0026 | 0.0031 | 0.0162 |
Mitochondria | 0.0078 | 0.0004 | 0.0006 | 0.0107 | 0.0003 | 0.0025 | 0.0118 | 0.0011 | 0.0087 | 0.0024 | 0.0353 | 0.0009 | 0.0023 | 0.0005 | 0.0016 | 0.0185 | 0.0623 | 0.0165 |
None | 0.2299 | 0.5749 | 0.7613 | 0.5708 | 0.6467 | 0.0515 | 0.2185 | 0.4611 | 0.3288 | 0.296 | 0.4156 | 0.1328 | 0.3925 | 0.4992 | 0.6394 | 0.531 | 0.5071 | 0.0865 |
Nuclear Periphery | 0.0487 | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0087 | 0.0146 | 0.001 | 0.0006 | 0.0004 | 0.0047 | 0.0003 | 0.0189 | 0.0004 | 0.0008 | 0.0006 | 0.0007 | 0.0012 |
Nucleolus | 0.0014 | 0.0007 | 0.0007 | 0.0011 | 0.0003 | 0.0003 | 0.0027 | 0.0015 | 0.0008 | 0.0004 | 0.0013 | 0.0007 | 0.0009 | 0.001 | 0.0006 | 0.0006 | 0.0003 | 0.0004 |
Nucleus | 0.0264 | 0.0012 | 0.0005 | 0.001 | 0.0004 | 0.0008 | 0.0163 | 0.0019 | 0.0018 | 0.0008 | 0.0029 | 0.001 | 0.0049 | 0.0019 | 0.001 | 0.0009 | 0.0006 | 0.0006 |
Peroxisomes | 0.0192 | 0.0004 | 0.0031 | 0.0045 | 0.0022 | 0.0017 | 0.0227 | 0.002 | 0.0075 | 0.0142 | 0.0047 | 0.0007 | 0.0056 | 0.0006 | 0.0039 | 0.0004 | 0.0008 | 0.0069 |
Punctate Nuclear | 0.0091 | 0.0017 | 0.002 | 0.0046 | 0.006 | 0.0024 | 0.0055 | 0.0024 | 0.0016 | 0.0011 | 0.0223 | 0.0009 | 0.0356 | 0.002 | 0.0011 | 0.0003 | 0.0011 | 0.0023 |
Vacuole | 0.0051 | 0.0023 | 0.0005 | 0.0009 | 0.0002 | 0.0013 | 0.0079 | 0.0032 | 0.0022 | 0.0009 | 0.0052 | 0.0014 | 0.0048 | 0.0018 | 0.0018 | 0.0235 | 0.0018 | 0.0008 |
Vacuole Periphery | 0.0034 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0006 | 0.0023 | 0.0002 | 0.0002 | 0.0002 | 0.0025 | 0.0001 | 0.0032 | 0.0001 | 0.0002 | 0.0091 | 0.0026 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.7024 | 13.3236 | 7.3353 | 9.9347 | 13.7139 | 10.5714 | 17.0566 | 15.8383 | 12.6308 | 16.8191 |
Translational Efficiency | 0.8108 | 1.0333 | 1.1087 | 0.7271 | 0.7412 | 0.7699 | 0.7585 | 0.641 | 0.6846 | 0.6947 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
257 | 80 | 169 | 1429 | 61 | 85 | 1242 | 1638 | 318 | 165 | 1411 | 3067 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 706.46 | 774.06 | 925.00 | 929.59 | 592.27 | 776.67 | 815.90 | 791.38 | 684.56 | 775.40 | 828.97 | 855.78 |
Standard Deviation | 90.56 | 90.27 | 106.35 | 134.74 | 74.83 | 116.48 | 123.84 | 111.25 | 98.61 | 104.60 | 126.92 | 140.79 |
Intensity Change Log 2 | — | 0.131838 | 0.388845 | 0.395987 | — | 0.391047 | 0.462137 | 0.418116 | — | 0.255846 | 0.422732 | 0.406120 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001177 | 0.007625 | 0.003584 | 0.023915 | 0.000559 | 0.001012 | 0.002643 | 0.002348 | 0.001059 | 0.004218 | 0.002756 | 0.012397 |
Bud Neck | 0.243272* | 0.137070 | 0.219287* | 0.208305* | 0.203417* | 0.102907 | 0.175127 | 0.280385* | 0.235627* | 0.119471 | 0.180416* | 0.246801* |
Bud Site | 0.169672* | 0.142622* | 0.244434* | 0.478874* | 0.052034 | 0.101558* | 0.210141* | 0.289797* | 0.147107* | 0.121468* | 0.214248* | 0.377893* |
Cell Periphery | 0.004933 | 0.005236 | 0.003590 | 0.009348 | 0.000999 | 0.000552 | 0.000988 | 0.002288 | 0.004178 | 0.002823 | 0.001300 | 0.005577 |
Cytoplasm | 0.256905* | 0.234155* | 0.147833* | 0.103725* | 0.274347* | 0.173416* | 0.303857* | 0.178417* | 0.260251* | 0.202865* | 0.285170* | 0.143616* |
Cytoplasmic Foci | 0.109968 | 0.192566* | 0.055001 | 0.016687 | 0.243002* | 0.208646* | 0.036380 | 0.017116 | 0.135487 | 0.200850* | 0.038610 | 0.016916 |
Eisosomes | 0.002410 | 0.001217 | 0.000567 | 0.001826 | 0.000554 | 0.000260 | 0.000263 | 0.000362 | 0.002054 | 0.000724 | 0.000300 | 0.001044 |
Endoplasmic Reticulum | 0.000973 | 0.002278 | 0.008437 | 0.004798 | 0.000636 | 0.015012 | 0.001061 | 0.000954 | 0.000908 | 0.008838 | 0.001944 | 0.002745 |
Endosome | 0.013619 | 0.057396 | 0.023276 | 0.007027 | 0.028181 | 0.078363 | 0.007642 | 0.005380 | 0.016413 | 0.068197 | 0.009514 | 0.006147 |
Golgi | 0.002012 | 0.013467 | 0.006196 | 0.004239 | 0.007544 | 0.011735 | 0.002991 | 0.001411 | 0.003073 | 0.012575 | 0.003375 | 0.002729 |
Lipid Particles | 0.005410 | 0.017511 | 0.007158 | 0.001248 | 0.009515 | 0.017698 | 0.001848 | 0.000686 | 0.006198 | 0.017607 | 0.002484 | 0.000948 |
Mitochondria | 0.001205 | 0.014642 | 0.000379 | 0.003361 | 0.041574 | 0.018094 | 0.000894 | 0.001580 | 0.008948 | 0.016420 | 0.000833 | 0.002410 |
Mitotic Spindle | 0.000439 | 0.001858 | 0.001027 | 0.007283 | 0.000504 | 0.008046 | 0.006274 | 0.004287 | 0.000452 | 0.005046 | 0.005646 | 0.005683 |
None | 0.003416 | 0.000538 | 0.000652 | 0.000186 | 0.002095 | 0.000449 | 0.006690 | 0.000512 | 0.003162 | 0.000492 | 0.005967 | 0.000360 |
Nuclear Periphery | 0.000154 | 0.000242 | 0.000805 | 0.000151 | 0.000178 | 0.001524 | 0.000260 | 0.000207 | 0.000159 | 0.000903 | 0.000325 | 0.000181 |
Nuclear Periphery Foci | 0.000307 | 0.001349 | 0.004427 | 0.001207 | 0.000526 | 0.001419 | 0.000643 | 0.000205 | 0.000349 | 0.001385 | 0.001096 | 0.000672 |
Nucleolus | 0.000626 | 0.001607 | 0.001079 | 0.000159 | 0.002171 | 0.003983 | 0.000495 | 0.000208 | 0.000923 | 0.002831 | 0.000565 | 0.000185 |
Nucleus | 0.058465 | 0.036351 | 0.110567 | 0.024615 | 0.068823 | 0.054621 | 0.086045 | 0.074083 | 0.060452 | 0.045763 | 0.088982 | 0.051035 |
Peroxisomes | 0.006395 | 0.077812 | 0.006557 | 0.008302 | 0.028172 | 0.036368 | 0.003551 | 0.002326 | 0.010572 | 0.056462 | 0.003911 | 0.005110 |
Vacuole | 0.117773 | 0.051846 | 0.154306 | 0.093654 | 0.034082 | 0.158826 | 0.151255 | 0.137142 | 0.101719 | 0.106957 | 0.151621 | 0.116880 |
Vacuole Periphery | 0.000869 | 0.002611 | 0.000839 | 0.001092 | 0.001089 | 0.005510 | 0.000953 | 0.000304 | 0.000911 | 0.004104 | 0.000939 | 0.000671 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.94 | -3.09 | -11.36 | -5.39 | -9.71 | -1.19 | -2.62 | -4.11 | -2.93 | -0.11 | -2.82 | -2.24 | -11.40 | -5.43 | -9.36 |
Bud Neck | 3.62 | 1.82 | 3.02 | -2.03 | 0.43 | 2.19 | 1.89 | -0.80 | -5.60 | -11.56 | 4.98 | 5.58 | 1.29 | -5.54 | -9.94 |
Bud Site | 0.95 | -1.63 | -12.67 | -11.53 | -9.15 | -1.56 | -5.86 | -10.99 | -5.96 | -8.67 | 1.26 | -0.91 | -11.12 | -11.97 | -18.40 |
Cell Periphery | -0.06 | 1.16 | -2.31 | -1.46 | -4.89 | 0.93 | 0.54 | -1.84 | -6.88 | -6.97 | 1.01 | 3.10 | -0.68 | -2.01 | -10.12 |
Cytoplasm | 0.84 | 4.61 | 8.35 | 4.01 | 1.57 | 2.18 | -0.30 | 2.22 | -0.55 | 9.05 | 2.44 | -0.68 | 6.80 | 2.31 | 12.55 |
Cytoplasmic Foci | -3.82 | 4.58 | 9.56 | 8.80 | 5.58 | 0.82 | 6.88 | 7.33 | 8.28 | 6.14 | -3.68 | 9.86 | 11.60 | 12.00 | 7.98 |
Eisosomes | 2.50 | 4.27 | 1.59 | -1.57 | -5.40 | 1.56 | 1.80 | 1.18 | -1.34 | -3.00 | 3.63 | 5.11 | 3.04 | -1.60 | -7.36 |
Endoplasmic Reticulum | -1.51 | -4.97 | -6.91 | -2.75 | 2.36 | -1.25 | -2.32 | -1.91 | 1.20 | 0.68 | -1.34 | -4.56 | -6.51 | 0.96 | -2.22 |
Endosome | -3.44 | -2.13 | 4.12 | 4.08 | 4.04 | -2.82 | 2.38 | 2.50 | 4.98 | 1.31 | -5.14 | 3.72 | 4.87 | 6.44 | 2.70 |
Golgi | -4.14 | -0.87 | -1.20 | 3.51 | 0.49 | -0.71 | 2.30 | 2.75 | 2.22 | 0.98 | -3.27 | 0.81 | 1.17 | 3.69 | 0.15 |
Lipid Particles | -1.69 | -2.38 | 5.34 | 2.20 | 6.59 | -0.98 | 3.68 | 4.09 | 2.20 | 3.25 | -2.15 | 5.08 | 7.15 | 3.12 | 4.62 |
Mitochondria | -2.47 | 2.75 | -1.68 | 2.11 | -2.60 | 1.14 | 2.18 | 2.14 | 1.50 | -1.67 | -0.96 | 2.25 | 1.84 | 2.28 | -2.81 |
Mitotic Spindle | -0.32 | 0.44 | -3.77 | -4.14 | -5.13 | -1.29 | -4.48 | -3.42 | 0.70 | 1.81 | -1.20 | -3.33 | -3.81 | -0.22 | 0.46 |
None | 1.10 | 1.06 | 1.21 | 1.72 | 2.57 | 2.15 | -2.17 | 1.99 | -0.65 | 4.02 | 1.27 | -0.61 | 1.31 | 0.61 | 4.18 |
Nuclear Periphery | -0.92 | -5.44 | -2.56 | -0.07 | 4.70 | -1.22 | -5.86 | -4.25 | 1.02 | 2.77 | -1.31 | -10.22 | -5.82 | 1.00 | 6.00 |
Nuclear Periphery Foci | -0.91 | -6.12 | -2.91 | -0.18 | 4.60 | -1.90 | -0.61 | 0.70 | 3.47 | 7.65 | -1.78 | -3.61 | -1.57 | 1.11 | 2.37 |
Nucleolus | -0.64 | -0.40 | 1.63 | 1.28 | 3.51 | -0.86 | 1.44 | 1.59 | 2.15 | 3.42 | -1.55 | 1.73 | 2.35 | 2.50 | 3.05 |
Nucleus | 2.00 | -4.26 | 3.59 | 0.34 | 6.20 | 0.70 | -1.71 | -1.76 | -3.20 | -0.13 | 1.43 | -5.55 | -1.21 | -2.68 | 6.64 |
Peroxisomes | -4.00 | 0.32 | -0.32 | 3.98 | -1.09 | -0.54 | 3.37 | 3.46 | 3.05 | 1.31 | -4.25 | 3.18 | 2.51 | 4.96 | -2.47 |
Vacuole | 3.41 | -3.54 | -2.35 | -5.86 | 2.38 | -4.96 | -15.05 | -10.57 | -0.86 | 7.92 | -0.86 | -13.56 | -6.81 | -3.51 | 10.36 |
Vacuole Periphery | -1.82 | 0.27 | -0.03 | 1.93 | -0.38 | -2.27 | 0.99 | 2.33 | 2.78 | 1.66 | -2.81 | 0.65 | 0.98 | 3.32 | 0.41 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|