Standard name
Description Dual-specificity kinase; autophosphorylation required for function; required for spindle pole body (SPB) duplication and spindle checkpoint function; contributes to bi-orientation by promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; substrates include SPB proteins Spc42p, Spc110p, and Spc98p, mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); similar to human Mps1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.19 0.24 0.23 0.18 0.11 0.23 0.1 0 0 0 0 0 0.16 0 0 0.18 0.09 0.15 0.11 0.11 0.1 0.13 0.18 0.1
Bud 0 0.09 0.06 0.05 0.08 0.12 0.07 0.06 0.06 0.07 0.06 0 0.06 0 0 0 0 0.06 0.14 0.18 0.14 0.18 0.05 0
Bud Neck 0.05 0.08 0.11 0.09 0.1 0.08 0.09 0.06 0 0 0 0.05 0 0 0 0.05 0.06 0 0.33 0.33 0.37 0.27 0.1 0.05
Bud Site 0.1 0.17 0.18 0.09 0.1 0.22 0.05 0 0 0 0 0 0.11 0 0 0.11 0.05 0.11 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.08 0.05
Cytoplasm 0.62 0.54 0.55 0.67 0.62 0.44 0.66 0.74 0.73 0.66 0.67 0.71 0.74 0.95 0.97 0.59 0.63 0.6 0.24 0.21 0.16 0.19 0.36 0.54
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0.08 0.12 0.1 0.25 0.34 0.4 0.2 0 0 0 0 0 0.06 0 0.05 0.08 0.07 0.13 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.09 0.24 0.12 0.18 0.2 0.08 0.07 0.06 0.05 0 0.05 0 0 0 0.15 0.15 0.14 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 51 75 47 30 71 35 10 5 7 3 9 36 5 1 40 14 19 11 27 35 25 40 24
Bud 3 19 20 14 22 35 24 22 26 23 16 5 13 5 1 7 5 7 14 46 52 37 12 9
Bud Neck 4 16 36 22 28 24 33 21 18 10 10 15 2 1 2 10 9 0 34 85 136 54 22 12
Bud Site 7 35 57 24 27 67 19 4 15 5 4 5 23 1 1 24 8 14 – – – – – –
Cell Periphery 1 8 6 10 1 7 6 4 3 2 1 2 1 4 4 4 8 1 1 7 14 9 16 13
Cytoplasm 45 113 175 174 172 132 233 275 294 206 174 198 162 247 207 130 95 75 24 53 59 38 81 138
Endoplasmic Reticulum 1 4 0 1 5 5 12 5 1 2 1 4 2 4 2 10 5 6 1 1 2 4 5 7
Endosome 1 6 1 0 0 0 0 1 1 0 0 0 0 0 0 4 6 4 3 4 8 5 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 0 0 2 1 1 1
Mitochondria 7 5 0 2 6 24 42 37 102 108 104 56 3 2 2 9 5 8 3 13 28 13 29 24
Nucleus 0 2 5 4 8 6 2 7 1 2 0 2 1 0 0 4 2 6 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 1 3 1 6 1 2 1 1 0 1 0 0 1 1 0 0 0 0 0 1
Peroxisomes 6 8 3 4 0 14 0 0 3 0 1 4 12 3 1 3 4 4 0 0 1 0 0 1
SpindlePole 1 19 77 32 50 60 29 27 24 15 7 14 3 3 0 33 23 17 2 2 9 3 0 2
Vac/Vac Membrane 0 1 0 0 3 1 0 5 2 1 3 2 0 0 0 4 3 2 3 10 11 4 10 11
Unique Cell Count 73 209 321 258 276 303 355 371 402 314 261 277 219 261 214 220 151 125 104 257 368 201 225 257
Labelled Cell Count 90 288 455 334 353 449 436 424 496 384 325 317 259 276 221 284 189 165 104 257 368 201 225 257


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.2 5.3 5.0 5.3 4.3 4.1 4.4 3.4 3.2 3.0 3.6 4.5 4.4 4.3 7.0 7.2 6.5 4.5 4.2 4.5
Std Deviation (1e-4) 0.9 1.0 1.2 1.4 1.7 1.2 0.9 1.1 0.7 1.0 0.7 0.7 1.0 1.4 1.6 1.7 1.8 1.9 1.4 1.5 2.0
Intensity Change (Log2) – – – -0.08 0.01 -0.29 -0.35 -0.25 -0.64 -0.73 -0.81 -0.57 -0.23 -0.26 -0.29 0.41 0.46 0.3 -0.23 -0.32 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.5 -4.0 0 0 0 0 0 0 0 0 0 0 -1.4 -3.7 -1.9
Bud -0.4 0.8 0 0 0 0 0 0 0 0 0 0 -1.6 -1.3 -0.3
Bud Neck -1.1 -0.4 0 0 0 0 0 0 0 0 0 0 -2.7 -1.8 -3.9
Bud Site -2.9 -2.8 0 0 0 0 0 0 0 0 0 0 -2.2 -3.7 -1.7
Cell Periphery 1.5 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 1.9 -2.7 3.0 5.4 5.2 2.9 3.0 4.3 4.6 10.8 10.6 1.1 1.7 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 1.1 0.4 0 0.3 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -3.5 -1.7 0 0 0 0 0 0 0 0 0 0 -2.6 -2.2 -2.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9075 -0.995 -0.9477 -0.9657 -2.6271 -0.1279 1.6086 -0.0125 -0.1904 -0.2231 -0.5246 0.5089 0.3991 -0.7265 -0.9677 -0.6991 -1.1267 0.0363
Actin 0.1106 0.0105 0.0068 0.0441 0.0037 0.0568 0.0846 0.0139 0.047 0.0545 0.0343 0.0288 0.0889 0.0101 0.0074 0.0003 0.008 0.0357
Bud 0.0018 0.0057 0.002 0.0083 0.0003 0.0004 0.0035 0.0032 0.0028 0.0032 0.0027 0.0016 0.0014 0.0051 0.0019 0.0005 0.001 0.0007
Bud Neck 0.1072 0.0087 0.038 0.1544 0.0882 0.7763 0.1239 0.0093 0.2073 0.4511 0.1985 0.6796 0.1208 0.0117 0.1082 0.2131 0.3217 0.6934
Bud Periphery 0.0028 0.0104 0.0059 0.0146 0.001 0.0012 0.0081 0.0063 0.0058 0.0227 0.017 0.0056 0.0022 0.0165 0.0029 0.0011 0.0027 0.0017
Bud Site 0.1202 0.1674 0.0752 0.0936 0.0018 0.0022 0.1266 0.264 0.1572 0.0191 0.0031 0.0066 0.1183 0.2539 0.0811 0.0092 0.0069 0.0028
Cell Periphery 0.022 0.0172 0.0035 0.0069 0.0016 0.0013 0.0236 0.0098 0.008 0.0019 0.0016 0.0046 0.0146 0.0174 0.0036 0.0006 0.0012 0.0012
Cytoplasm 0.0887 0.1805 0.0545 0.0115 0.046 0.0366 0.1912 0.1909 0.1807 0.0706 0.1156 0.1247 0.082 0.1637 0.0966 0.11 0.0464 0.0907
Cytoplasmic Foci 0.0489 0.0091 0.035 0.0596 0.1903 0.016 0.0377 0.0109 0.0186 0.0242 0.0616 0.0051 0.0565 0.0072 0.0313 0.0117 0.0146 0.0154
Eisosomes 0.0025 0.0013 0.0022 0.0011 0.0013 0.0005 0.0013 0.001 0.0006 0.0003 0.0011 0.0004 0.0018 0.0011 0.0012 0.0001 0.0005 0.0004
Endoplasmic Reticulum 0.0153 0.0033 0.0009 0.001 0.0007 0.0121 0.0099 0.0067 0.0022 0.0013 0.0036 0.0016 0.0047 0.003 0.0044 0.0012 0.0011 0.0009
Endosome 0.0683 0.0016 0.0033 0.0018 0.0046 0.009 0.0269 0.0047 0.0061 0.0069 0.0496 0.0015 0.0277 0.0009 0.0052 0.0553 0.0078 0.0121
Golgi 0.0108 0.0005 0.0018 0.005 0.0022 0.0078 0.0122 0.0011 0.006 0.0114 0.0085 0.001 0.0045 0.0006 0.0028 0.0092 0.0076 0.0131
Lipid Particles 0.05 0.0016 0.002 0.0034 0.0022 0.0099 0.0483 0.0038 0.0054 0.0163 0.008 0.0004 0.008 0.0012 0.003 0.0026 0.0031 0.0162
Mitochondria 0.0078 0.0004 0.0006 0.0107 0.0003 0.0025 0.0118 0.0011 0.0087 0.0024 0.0353 0.0009 0.0023 0.0005 0.0016 0.0185 0.0623 0.0165
None 0.2299 0.5749 0.7613 0.5708 0.6467 0.0515 0.2185 0.4611 0.3288 0.296 0.4156 0.1328 0.3925 0.4992 0.6394 0.531 0.5071 0.0865
Nuclear Periphery 0.0487 0.0005 0.0002 0.0005 0.0001 0.0087 0.0146 0.001 0.0006 0.0004 0.0047 0.0003 0.0189 0.0004 0.0008 0.0006 0.0007 0.0012
Nucleolus 0.0014 0.0007 0.0007 0.0011 0.0003 0.0003 0.0027 0.0015 0.0008 0.0004 0.0013 0.0007 0.0009 0.001 0.0006 0.0006 0.0003 0.0004
Nucleus 0.0264 0.0012 0.0005 0.001 0.0004 0.0008 0.0163 0.0019 0.0018 0.0008 0.0029 0.001 0.0049 0.0019 0.001 0.0009 0.0006 0.0006
Peroxisomes 0.0192 0.0004 0.0031 0.0045 0.0022 0.0017 0.0227 0.002 0.0075 0.0142 0.0047 0.0007 0.0056 0.0006 0.0039 0.0004 0.0008 0.0069
Punctate Nuclear 0.0091 0.0017 0.002 0.0046 0.006 0.0024 0.0055 0.0024 0.0016 0.0011 0.0223 0.0009 0.0356 0.002 0.0011 0.0003 0.0011 0.0023
Vacuole 0.0051 0.0023 0.0005 0.0009 0.0002 0.0013 0.0079 0.0032 0.0022 0.0009 0.0052 0.0014 0.0048 0.0018 0.0018 0.0235 0.0018 0.0008
Vacuole Periphery 0.0034 0.0001 0.0001 0.0003 0 0.0006 0.0023 0.0002 0.0002 0.0002 0.0025 0.0001 0.0032 0.0001 0.0002 0.0091 0.0026 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.7024 13.3236 7.3353 9.9347 13.7139 10.5714 17.0566 15.8383 12.6308 16.8191
Translational Efficiency 0.8108 1.0333 1.1087 0.7271 0.7412 0.7699 0.7585 0.641 0.6846 0.6947

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘