Standard name
Description Protein that associates with mitochondrial ribosome; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.05 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0 0 0 0 0 0
Cytoplasm 0.08 0.11 0.11 0.13 0.08 0 0 0 0 0 0 0 0.58 0.6 0.64 0.12 0.11 0.12 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0.08 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0.11 0 0.1 0.08 0.14 0 0 0 0 0 0 0 0.06 0.1 0.11 0.18 0.21 0.27 0.13 0.12 0.1 0.05 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.15 0.11 0 0 0 0 0 0
Mitochondria 0.78 0.91 0.72 0.78 0.78 0.94 0.91 0.9 0.93 0.97 0.96 0.95 0.09 0.08 0.08 0.16 0.13 0.08 0.65 0.65 0.67 0.7 0.72 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.13 0.16 0.1 0 0 0 0 0 0 0 0 0 0 0.14 0.1 0.14 0 0 0.08 0 0 0
SpindlePole 0.06 0 0.05 0.06 0 0 0 0.06 0 0 0 0 0.05 0 0.05 0.07 0.13 0.15 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.11 0.06 0 0.1 0.06 0 0.05 0 0 0 0.05 0.1 0.1 0.06 0.21 0.15 0.21 0 0.06 0 0.07 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 4 3 0 0 0 0 0 0 0 0 0 13 5 7 3 5 1 2 2 13 2 11 14
Bud 6 1 6 8 11 14 22 15 23 25 27 25 1 3 2 5 8 1 1 1 0 2 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 1 3 1 0 2 0 2 0 1 1 3 3
Bud Site 0 0 0 1 1 2 8 8 3 9 13 9 0 0 1 1 0 2
Cell Periphery 1 0 10 5 4 1 4 1 1 2 0 4 5 10 7 17 14 7 0 0 0 1 0 2
Cytoplasm 18 8 28 28 27 11 23 12 15 6 11 10 89 135 173 27 20 16 1 0 1 12 13 10
Endoplasmic Reticulum 4 6 8 1 0 4 2 0 1 2 0 3 9 8 9 9 12 5 5 0 2 2 1 2
Endosome 24 0 27 16 50 5 5 10 6 0 2 0 9 23 31 42 38 35 36 13 29 12 19 25
Golgi 0 0 1 0 1 0 0 0 0 0 0 0 0 2 1 32 27 14 7 0 11 1 4 9
Mitochondria 170 68 191 162 270 353 519 472 426 555 430 480 14 17 21 38 23 10 178 69 201 175 294 304
Nucleus 3 0 1 0 4 6 16 17 6 13 7 9 1 1 2 10 1 4 0 0 0 3 2 3
Nuclear Periphery 0 1 0 1 0 17 23 17 8 21 6 15 0 0 0 1 1 0 5 0 2 0 0 3
Nucleolus 6 1 0 0 2 4 17 14 15 11 9 7 0 0 0 2 1 0 1 0 1 0 2 1
Peroxisomes 12 0 34 33 35 3 7 12 11 11 2 4 2 0 5 32 17 18 7 2 24 2 10 13
SpindlePole 13 0 13 12 13 13 15 31 19 19 8 21 7 10 14 17 24 20 4 2 4 9 8 11
Vac/Vac Membrane 16 8 15 5 36 21 13 27 20 20 14 24 15 22 15 48 27 27 11 6 1 17 25 22
Unique Cell Count 219 75 265 208 348 377 571 527 459 575 449 503 153 225 270 234 178 131 274 107 303 252 410 439
Labelled Cell Count 273 97 337 272 454 454 674 636 554 695 529 611 166 239 289 284 220 160 274 107 303 252 410 439


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.3 4.6 4.2 4.9 4.0 4.0 3.9 3.9 3.7 3.4 3.5 5.6 5.6 5.5 6.8 6.7 6.3 5.3 5.8 6.0
Std Deviation (1e-4) 0.8 0.5 1.4 1.8 2.3 1.3 1.6 1.2 1.5 0.9 1.0 1.1 0.8 1.1 1.0 1.3 1.4 1.3 1.0 1.2 1.5
Intensity Change (Log2) -0.14 0.08 -0.21 -0.21 -0.23 -0.26 -0.32 -0.45 -0.4 0.27 0.28 0.25 0.56 0.54 0.45 0.19 0.33 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 5.0 3.3 4.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available