| Standard name | |
|---|---|
| Description | Protein that associates with mitochondrial ribosome; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL027C is not an essential gene |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 13 | 5 | 7 | 3 | 5 | 1 | 2 | 2 | 13 | 2 | 11 | 14 |
| Bud | 6 | 1 | 6 | 8 | 11 | 14 | 22 | 15 | 23 | 25 | 27 | 25 | 1 | 3 | 2 | 5 | 8 | 1 | 1 | 1 | 0 | 2 | 3 | 4 |
| Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 0 | 2 | 0 | 2 | 0 | 1 | 1 | 3 | 3 |
| Bud Site | 0 | 0 | 0 | 1 | 1 | 2 | 8 | 8 | 3 | 9 | 13 | 9 | 0 | 0 | 1 | 1 | 0 | 2 | – | – | – | – | – | – |
| Cell Periphery | 1 | 0 | 10 | 5 | 4 | 1 | 4 | 1 | 1 | 2 | 0 | 4 | 5 | 10 | 7 | 17 | 14 | 7 | 0 | 0 | 0 | 1 | 0 | 2 |
| Cytoplasm | 18 | 8 | 28 | 28 | 27 | 11 | 23 | 12 | 15 | 6 | 11 | 10 | 89 | 135 | 173 | 27 | 20 | 16 | 1 | 0 | 1 | 12 | 13 | 10 |
| Endoplasmic Reticulum | 4 | 6 | 8 | 1 | 0 | 4 | 2 | 0 | 1 | 2 | 0 | 3 | 9 | 8 | 9 | 9 | 12 | 5 | 5 | 0 | 2 | 2 | 1 | 2 |
| Endosome | 24 | 0 | 27 | 16 | 50 | 5 | 5 | 10 | 6 | 0 | 2 | 0 | 9 | 23 | 31 | 42 | 38 | 35 | 36 | 13 | 29 | 12 | 19 | 25 |
| Golgi | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 32 | 27 | 14 | 7 | 0 | 11 | 1 | 4 | 9 |
| Mitochondria | 170 | 68 | 191 | 162 | 270 | 353 | 519 | 472 | 426 | 555 | 430 | 480 | 14 | 17 | 21 | 38 | 23 | 10 | 178 | 69 | 201 | 175 | 294 | 304 |
| Nucleus | 3 | 0 | 1 | 0 | 4 | 6 | 16 | 17 | 6 | 13 | 7 | 9 | 1 | 1 | 2 | 10 | 1 | 4 | 0 | 0 | 0 | 3 | 2 | 3 |
| Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 17 | 23 | 17 | 8 | 21 | 6 | 15 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | 0 | 2 | 0 | 0 | 3 |
| Nucleolus | 6 | 1 | 0 | 0 | 2 | 4 | 17 | 14 | 15 | 11 | 9 | 7 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 1 |
| Peroxisomes | 12 | 0 | 34 | 33 | 35 | 3 | 7 | 12 | 11 | 11 | 2 | 4 | 2 | 0 | 5 | 32 | 17 | 18 | 7 | 2 | 24 | 2 | 10 | 13 |
| SpindlePole | 13 | 0 | 13 | 12 | 13 | 13 | 15 | 31 | 19 | 19 | 8 | 21 | 7 | 10 | 14 | 17 | 24 | 20 | 4 | 2 | 4 | 9 | 8 | 11 |
| Vac/Vac Membrane | 16 | 8 | 15 | 5 | 36 | 21 | 13 | 27 | 20 | 20 | 14 | 24 | 15 | 22 | 15 | 48 | 27 | 27 | 11 | 6 | 1 | 17 | 25 | 22 |
| Unique Cell Count | 219 | 75 | 265 | 208 | 348 | 377 | 571 | 527 | 459 | 575 | 449 | 503 | 153 | 225 | 270 | 234 | 178 | 131 | 274 | 107 | 303 | 252 | 410 | 439 |
| Labelled Cell Count | 273 | 97 | 337 | 272 | 454 | 454 | 674 | 636 | 554 | 695 | 529 | 611 | 166 | 239 | 289 | 284 | 220 | 160 | 274 | 107 | 303 | 252 | 410 | 439 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.0 | 4.3 | 4.6 | 4.2 | 4.9 | 4.0 | 4.0 | 3.9 | 3.9 | 3.7 | 3.4 | 3.5 | 5.6 | 5.6 | 5.5 | 6.8 | 6.7 | 6.3 | 5.3 | 5.8 | 6.0 |
| Std Deviation (1e-4) | 0.8 | 0.5 | 1.4 | 1.8 | 2.3 | 1.3 | 1.6 | 1.2 | 1.5 | 0.9 | 1.0 | 1.1 | 0.8 | 1.1 | 1.0 | 1.3 | 1.4 | 1.3 | 1.0 | 1.2 | 1.5 |
| Intensity Change (Log2) | – | – | – | -0.14 | 0.08 | -0.21 | -0.21 | -0.23 | -0.26 | -0.32 | -0.45 | -0.4 | 0.27 | 0.28 | 0.25 | 0.56 | 0.54 | 0.45 | 0.19 | 0.33 | 0.36 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.0 | 3.3 | 4.5 |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Mrx9-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
