Standard name | |
---|---|
Human Ortholog | |
Description | NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases Ypk1p and Ypk2p, dephosphorylation increases catalytic activity; forms a heterodimer with Pnc1p to facilitate its peroxisomal import |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 2 | 0 | 8 | 9 | 34 | 29 | 44 | 47 | 74 | 89 | 15 | 30 | 30 | 22 | 25 | 20 | 0 | – | 67 | 29 | 28 | 40 |
Bud | – | – | 6 | 6 | 15 | 33 | 54 | 34 | 45 | 56 | 85 | 63 | 4 | 4 | 5 | 7 | 4 | 11 | 0 | – | 8 | 4 | 3 | 11 |
Bud Neck | – | – | 10 | 4 | 4 | 2 | 2 | 0 | 2 | 0 | 0 | 1 | 2 | 3 | 3 | 3 | 4 | 3 | 0 | – | 11 | 9 | 15 | 11 |
Bud Site | – | – | 0 | 2 | 2 | 3 | 2 | 3 | 4 | 7 | 10 | 13 | 0 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 10 | 23 | 21 | 21 | 27 | 36 | 36 | 37 | 56 | 78 | 22 | 51 | 57 | 26 | 19 | 10 | 0 | – | 28 | 16 | 26 | 39 |
Cytoplasm | – | – | 337 | 332 | 344 | 278 | 238 | 225 | 156 | 114 | 138 | 163 | 371 | 344 | 291 | 278 | 261 | 171 | 0 | – | 109 | 159 | 122 | 224 |
Endoplasmic Reticulum | – | – | 1 | 2 | 9 | 32 | 54 | 40 | 51 | 65 | 60 | 100 | 11 | 25 | 52 | 19 | 28 | 21 | 0 | – | 13 | 12 | 13 | 15 |
Endosome | – | – | 3 | 0 | 8 | 2 | 3 | 18 | 7 | 2 | 12 | 10 | 1 | 2 | 2 | 4 | 3 | 2 | 0 | – | 8 | 2 | 4 | 5 |
Golgi | – | – | 0 | 0 | 1 | 1 | 0 | 6 | 8 | 6 | 13 | 18 | 2 | 2 | 6 | 1 | 1 | 2 | 0 | – | 0 | 1 | 1 | 3 |
Mitochondria | – | – | 12 | 41 | 91 | 320 | 431 | 298 | 312 | 383 | 454 | 463 | 2 | 4 | 3 | 11 | 21 | 6 | 0 | – | 114 | 47 | 31 | 48 |
Nucleus | – | – | 1 | 1 | 0 | 2 | 3 | 4 | 1 | 2 | 4 | 3 | 0 | 0 | 3 | 6 | 3 | 0 | 0 | – | 0 | 1 | 1 | 0 |
Nuclear Periphery | – | – | 0 | 1 | 0 | 2 | 6 | 16 | 12 | 14 | 9 | 19 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 1 |
Nucleolus | – | – | 0 | 0 | 1 | 1 | 2 | 3 | 1 | 3 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 2 | 14 | 12 | 2 | 0 | 17 | 5 | 6 | 10 | 14 | 0 | 4 | 1 | 5 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 |
SpindlePole | – | – | 7 | 11 | 12 | 2 | 12 | 17 | 16 | 9 | 20 | 14 | 2 | 6 | 13 | 13 | 2 | 5 | 0 | – | 4 | 1 | 2 | 4 |
Vac/Vac Membrane | – | – | 1 | 3 | 6 | 7 | 27 | 46 | 28 | 23 | 30 | 41 | 1 | 4 | 3 | 8 | 2 | 1 | 0 | – | 4 | 7 | 9 | 5 |
Unique Cell Count | 364 | 376 | 452 | 561 | 677 | 597 | 528 | 555 | 678 | 755 | 406 | 429 | 403 | 353 | 319 | 218 | 1 | 387 | 300 | 271 | 418 | |||
Labelled Cell Count | 392 | 440 | 534 | 717 | 895 | 792 | 728 | 774 | 980 | 1093 | 433 | 480 | 469 | 403 | 373 | 254 | 1 | 387 | 300 | 271 | 418 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 4.0 | 4.1 | 4.9 | 3.6 | 4.1 | 5.0 | 5.3 | 6.1 | 6.7 | 7.2 | 5.8 | 6.7 | 7.3 | 5.9 | 5.7 | 5.4 | 5.5 | 5.7 | 5.9 |
Std Deviation (1e-4) | – | – | 1.5 | 3.3 | 5.5 | 2.1 | 1.9 | 3.2 | 3.6 | 4.6 | 5.9 | 6.4 | 2.1 | 3.0 | 3.7 | 1.4 | 1.6 | 1.9 | 1.5 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.31 | -0.13 | 0.04 | 0.33 | 0.43 | 0.62 | 0.76 | 0.87 | 0.54 | 0.76 | 0.88 | 0.57 | 0.53 | 0.44 | 0.48 | 0.52 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.7 | 5.2 | 5.2 | 6.1 | 6.4 | 3.0 | 4.6 | 4.8 | 4.2 | 4.9 | 5.3 |
Bud | 0 | 1.5 | 0 | 0 | 3.0 | 4.3 | 5.0 | 5.9 | 4.4 | 0 | 0 | -0.5 | 0.3 | 0 | 2.4 |
Bud Neck | 0 | -2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.5 | -2.3 | -2.1 | -1.9 | -1.4 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.4 | 0 | 0 | 2.3 | 2.7 | 2.6 | 3.5 | 4.4 | 1.9 | 4.8 | 5.6 | 2.8 | 2.1 | 1.2 |
Cytoplasm | -2.0 | -6.3 | -13.5 | -17.8 | -16.8 | -18.6 | -21.4 | -22.3 | -22.4 | -0.6 | -5.0 | -7.3 | -5.3 | -4.3 | -5.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 4.8 | 5.9 | 6.6 | 5.6 | 7.1 | 2.7 | 4.4 | 6.9 | 4.2 | 5.5 | 5.7 |
Endosome | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 7.2 | 0 | 0 | 15.0 | 17.0 | 19.7 | 19.7 | 18.4 | -2.9 | -2.4 | -2.5 | -0.1 | 2.0 | -0.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 3.1 | 0 | 3.1 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 2.3 | 0 | 0 | 2.5 | 0 | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0.7 | 0 | 0 | 0.9 | 1.0 | -0.3 | 1.0 | -0.1 | 0 | -0.6 | 1.1 | 1.4 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.5 | 5.2 | 4.2 | 3.6 | 3.8 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2799 | 1.4244 | 1.3159 | 1.2886 | 1.8413 | 1.6422 | 1.8073 | 2.2596 | 2.4736 | 2.477 | 1.9809 | 2.0146 | 1.2076 | 1.2442 | 0.6223 | 0.6779 | 0.7571 | 0.5248 |
Actin | 0.0437 | 0.0339 | 0.0529 | 0.0171 | 0.0112 | 0.0391 | 0.1085 | 0.0583 | 0.1439 | 0.0611 | 0.0997 | 0.0763 | 0.0901 | 0.0622 | 0.0971 | 0.1239 | 0.1336 | 0.0942 |
Bud | 0.0046 | 0.0081 | 0.0124 | 0.0056 | 0.0021 | 0.0028 | 0.0064 | 0.0113 | 0.0152 | 0.017 | 0.0138 | 0.0096 | 0.0058 | 0.0092 | 0.0154 | 0.0179 | 0.0089 | 0.004 |
Bud Neck | 0.0082 | 0.0054 | 0.0072 | 0.0026 | 0.0098 | 0.0382 | 0.0117 | 0.0075 | 0.0179 | 0.013 | 0.0077 | 0.1221 | 0.0162 | 0.0065 | 0.0088 | 0.0095 | 0.0374 | 0.0928 |
Bud Periphery | 0.0296 | 0.0156 | 0.0533 | 0.0486 | 0.0329 | 0.0267 | 0.0314 | 0.0323 | 0.0832 | 0.1392 | 0.0728 | 0.0613 | 0.0328 | 0.0228 | 0.0707 | 0.1401 | 0.0419 | 0.0335 |
Bud Site | 0.0251 | 0.1489 | 0.0475 | 0.012 | 0.0032 | 0.0067 | 0.0402 | 0.1935 | 0.081 | 0.032 | 0.0204 | 0.0071 | 0.0404 | 0.2586 | 0.0695 | 0.0218 | 0.0104 | 0.0048 |
Cell Periphery | 0.0497 | 0.0358 | 0.0391 | 0.045 | 0.1389 | 0.0711 | 0.0904 | 0.0904 | 0.1221 | 0.1319 | 0.0391 | 0.0987 | 0.144 | 0.0724 | 0.1044 | 0.1301 | 0.0866 | 0.0965 |
Cytoplasm | 0.2104 | 0.1743 | 0.1904 | 0.2805 | 0.3396 | 0.2555 | 0.2888 | 0.3407 | 0.2537 | 0.3061 | 0.2525 | 0.4294 | 0.2756 | 0.2877 | 0.2458 | 0.239 | 0.2741 | 0.3375 |
Cytoplasmic Foci | 0.0151 | 0.011 | 0.0187 | 0.0132 | 0.0229 | 0.0191 | 0.0425 | 0.0122 | 0.0195 | 0.0068 | 0.0367 | 0.0176 | 0.0309 | 0.0063 | 0.0211 | 0.0349 | 0.0168 | 0.0197 |
Eisosomes | 0.0064 | 0.0023 | 0.0038 | 0.0021 | 0.0052 | 0.0026 | 0.0155 | 0.0063 | 0.0096 | 0.0089 | 0.0033 | 0.0059 | 0.0201 | 0.0073 | 0.01 | 0.0058 | 0.0047 | 0.0063 |
Endoplasmic Reticulum | 0.0239 | 0.0028 | 0.0047 | 0.0089 | 0.0054 | 0.0068 | 0.0219 | 0.0203 | 0.0115 | 0.0244 | 0.0089 | 0.019 | 0.0225 | 0.0063 | 0.0115 | 0.013 | 0.0113 | 0.0118 |
Endosome | 0.0043 | 0.0021 | 0.0047 | 0.0036 | 0.0061 | 0.0089 | 0.03 | 0.0049 | 0.0153 | 0.0087 | 0.0625 | 0.0108 | 0.026 | 0.0023 | 0.0135 | 0.0064 | 0.0384 | 0.0119 |
Golgi | 0.0038 | 0.0013 | 0.0041 | 0.0017 | 0.0027 | 0.0055 | 0.018 | 0.0064 | 0.0147 | 0.0136 | 0.0315 | 0.0071 | 0.0172 | 0.0033 | 0.0107 | 0.0094 | 0.0292 | 0.0116 |
Lipid Particles | 0.0043 | 0.0012 | 0.0013 | 0.0014 | 0.0027 | 0.0021 | 0.0166 | 0.0036 | 0.0076 | 0.0058 | 0.0219 | 0.0059 | 0.0177 | 0.001 | 0.0115 | 0.0101 | 0.0475 | 0.0105 |
Mitochondria | 0.0033 | 0.0012 | 0.0038 | 0.0039 | 0.0111 | 0.0035 | 0.0214 | 0.0008 | 0.0063 | 0.0156 | 0.0426 | 0.0022 | 0.0064 | 0.0016 | 0.0054 | 0.0148 | 0.0392 | 0.007 |
None | 0.5578 | 0.5378 | 0.5389 | 0.5475 | 0.3941 | 0.5041 | 0.2065 | 0.2046 | 0.1751 | 0.2049 | 0.2472 | 0.1136 | 0.2184 | 0.2479 | 0.2853 | 0.2027 | 0.1797 | 0.2331 |
Nuclear Periphery | 0.0014 | 0.0009 | 0.0007 | 0.0006 | 0.0004 | 0.0006 | 0.0148 | 0.0009 | 0.004 | 0.0021 | 0.0062 | 0.0033 | 0.0085 | 0.0004 | 0.0017 | 0.001 | 0.0088 | 0.0034 |
Nucleolus | 0.0006 | 0.0006 | 0.0024 | 0.0005 | 0.0003 | 0.0003 | 0.0017 | 0.0004 | 0.0007 | 0.0005 | 0.002 | 0.0005 | 0.0009 | 0.0003 | 0.0012 | 0.0003 | 0.0008 | 0.0004 |
Nucleus | 0.0012 | 0.0047 | 0.0031 | 0.0013 | 0.0006 | 0.0016 | 0.0054 | 0.001 | 0.0016 | 0.0012 | 0.0032 | 0.0018 | 0.0041 | 0.0007 | 0.0014 | 0.0008 | 0.0014 | 0.0043 |
Peroxisomes | 0.001 | 0.0026 | 0.0022 | 0.0012 | 0.0084 | 0.0013 | 0.0084 | 0.0005 | 0.0047 | 0.0021 | 0.0068 | 0.0007 | 0.0092 | 0.0003 | 0.0063 | 0.0146 | 0.0099 | 0.0132 |
Punctate Nuclear | 0.0008 | 0.0036 | 0.0043 | 0.0008 | 0.0004 | 0.0013 | 0.0101 | 0.0005 | 0.0081 | 0.0005 | 0.0057 | 0.0012 | 0.0049 | 0.0002 | 0.0022 | 0.0008 | 0.0074 | 0.0012 |
Vacuole | 0.0044 | 0.0057 | 0.0039 | 0.0017 | 0.0018 | 0.0017 | 0.0075 | 0.0035 | 0.0038 | 0.0041 | 0.0129 | 0.0054 | 0.0073 | 0.0025 | 0.0058 | 0.0025 | 0.007 | 0.0019 |
Vacuole Periphery | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0022 | 0.0002 | 0.0006 | 0.0008 | 0.0026 | 0.0006 | 0.001 | 0.0002 | 0.0006 | 0.0004 | 0.0049 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 68.4936 | 52.8416 | 82.1558 | 129.0559 | 60.8253 | 128.4717 | 99.8596 | 125.3526 | 197.7931 | 84.452 |
Translational Efficiency | 1.7246 | 1.5433 | 1.2882 | 1.891 | 1.9835 | 1.4559 | 1.6743 | 1.782 | 1.7372 | 1.8498 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1696 | 229 | 1394 | 1073 | 1904 | 1463 | 88 | 881 | 3600 | 1692 | 1482 | 1954 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 665.53 | 741.74 | 797.66 | 792.15 | 674.89 | 695.53 | 897.62 | 799.56 | 670.48 | 701.78 | 803.60 | 795.49 |
Standard Deviation | 70.35 | 87.12 | 100.44 | 130.68 | 72.44 | 79.20 | 120.94 | 242.97 | 71.61 | 81.86 | 104.48 | 189.76 |
Intensity Change Log 2 | — | 0.156410 | 0.261270 | 0.251270 | — | 0.043460 | 0.411452 | 0.244554 | — | 0.100646 | 0.338838 | 0.247892 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000124 | 0.000905 | 0.002909 | 0.020017 | 0.000713 | 0.000184 | 0.006507 | 0.037736 | 0.000436 | 0.000281 | 0.003122 | 0.028006 |
Bud Neck | 0.018596 | 0.041285 | 0.006656 | 0.014096 | 0.013598 | 0.028190 | 0.005137 | 0.013710 | 0.015953 | 0.029963 | 0.006566 | 0.013922 |
Bud Site | 0.004320 | 0.021556 | 0.013094 | 0.078270 | 0.006166 | 0.006316 | 0.015769 | 0.093335 | 0.005297 | 0.008378 | 0.013253 | 0.085062 |
Cell Periphery | 0.000132 | 0.000213 | 0.002378 | 0.019498 | 0.001194 | 0.000171 | 0.009201 | 0.046341 | 0.000694 | 0.000176 | 0.002783 | 0.031601 |
Cytoplasm | 0.441785* | 0.303818* | 0.395050* | 0.382417* | 0.542586* | 0.447513* | 0.285151* | 0.400189* | 0.495098* | 0.428065* | 0.388525* | 0.390430* |
Cytoplasmic Foci | 0.261088* | 0.314630* | 0.027440 | 0.050477 | 0.185486* | 0.231769* | 0.029710 | 0.056305 | 0.221102* | 0.242984* | 0.027575 | 0.053104 |
Eisosomes | 0.000228 | 0.000259 | 0.000301 | 0.004079 | 0.000594 | 0.000201 | 0.005791 | 0.008069 | 0.000421 | 0.000209 | 0.000627 | 0.005878 |
Endoplasmic Reticulum | 0.000694 | 0.000714 | 0.003523 | 0.013485 | 0.001097 | 0.000489 | 0.015064 | 0.018352 | 0.000908 | 0.000519 | 0.004208 | 0.015679 |
Endosome | 0.009670 | 0.042550 | 0.002754 | 0.004153 | 0.005285 | 0.016360 | 0.004869 | 0.003079 | 0.007351 | 0.019904 | 0.002879 | 0.003668 |
Golgi | 0.002804 | 0.004511 | 0.000361 | 0.002331 | 0.002195 | 0.003318 | 0.000633 | 0.003048 | 0.002482 | 0.003480 | 0.000377 | 0.002654 |
Lipid Particles | 0.010906 | 0.008991 | 0.003768 | 0.004850 | 0.007296 | 0.008301 | 0.007028 | 0.005328 | 0.008997 | 0.008394 | 0.003962 | 0.005065 |
Mitochondria | 0.006618 | 0.009310 | 0.000416 | 0.002178 | 0.003418 | 0.008756 | 0.000210 | 0.001282 | 0.004926 | 0.008831 | 0.000403 | 0.001774 |
Mitotic Spindle | 0.001875 | 0.002709 | 0.000926 | 0.009467 | 0.000141 | 0.004644 | 0.000169 | 0.002876 | 0.000958 | 0.004382 | 0.000881 | 0.006496 |
None | 0.002059 | 0.000985 | 0.005225 | 0.001766 | 0.002416 | 0.002608 | 0.027693 | 0.001873 | 0.002248 | 0.002388 | 0.006559 | 0.001814 |
Nuclear Periphery | 0.000460 | 0.000349 | 0.001172 | 0.000651 | 0.000279 | 0.000481 | 0.001521 | 0.000350 | 0.000364 | 0.000463 | 0.001192 | 0.000515 |
Nuclear Periphery Foci | 0.000203 | 0.000435 | 0.000836 | 0.002040 | 0.000202 | 0.000227 | 0.002079 | 0.001598 | 0.000203 | 0.000255 | 0.000910 | 0.001841 |
Nucleolus | 0.001191 | 0.002098 | 0.000542 | 0.000225 | 0.000592 | 0.001401 | 0.001138 | 0.000204 | 0.000874 | 0.001496 | 0.000577 | 0.000215 |
Nucleus | 0.186564* | 0.104772 | 0.488916* | 0.208939* | 0.192834* | 0.159306* | 0.430158* | 0.173739* | 0.189880* | 0.151925* | 0.485427* | 0.193069* |
Peroxisomes | 0.007777 | 0.042752 | 0.000456 | 0.003073 | 0.002886 | 0.010604 | 0.000685 | 0.005607 | 0.005190 | 0.014955 | 0.000470 | 0.004215 |
Vacuole | 0.041947 | 0.094485 | 0.043083 | 0.176724 | 0.030336 | 0.067642 | 0.151330 | 0.126639 | 0.035806 | 0.071275 | 0.049510 | 0.154142 |
Vacuole Periphery | 0.000957 | 0.002672 | 0.000196 | 0.001265 | 0.000685 | 0.001519 | 0.000156 | 0.000340 | 0.000813 | 0.001675 | 0.000193 | 0.000848 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.86 | -3.33 | -7.74 | -7.15 | -5.93 | 2.49 | -1.65 | -8.36 | -8.53 | -6.29 | 1.24 | -3.14 | -11.16 | -11.26 | -9.30 |
Bud Neck | -3.74 | 7.42 | 4.31 | 4.97 | -3.48 | -6.79 | 6.93 | 1.34 | 7.47 | -4.85 | -7.02 | 7.57 | 3.95 | 9.28 | -4.15 |
Bud Site | -3.91 | -3.68 | -11.03 | -5.82 | -8.92 | -0.12 | -0.92 | -11.58 | -11.61 | -8.30 | -2.90 | -3.40 | -15.92 | -14.95 | -12.93 |
Cell Periphery | -2.14 | -2.83 | -5.24 | -5.20 | -4.29 | 3.14 | -1.33 | -7.31 | -7.55 | -5.74 | 2.99 | -2.25 | -8.91 | -9.14 | -8.02 |
Cytoplasm | 7.66 | 6.70 | 8.80 | -2.51 | 2.20 | 10.96 | 11.69 | 15.05 | 5.92 | -5.56 | 8.74 | 15.20 | 17.61 | 7.23 | 0.33 |
Cytoplasmic Foci | -3.25 | 41.46 | 36.81 | 18.54 | -7.12 | -6.70 | 25.52 | 26.03 | 29.44 | -4.31 | -3.37 | 51.96 | 44.48 | 36.26 | -9.50 |
Eisosomes | -0.79 | -0.59 | -5.49 | -5.42 | -5.32 | 3.34 | -1.04 | -6.40 | -6.91 | -1.40 | 3.34 | -0.05 | -8.27 | -8.79 | -7.93 |
Endoplasmic Reticulum | -0.18 | -8.14 | -8.00 | -7.98 | -5.39 | 2.43 | -2.60 | -7.09 | -7.43 | -1.13 | 2.54 | -7.69 | -10.48 | -10.86 | -7.19 |
Endosome | -5.67 | 8.75 | 7.33 | 6.81 | -1.70 | -9.87 | -0.23 | 5.65 | 12.54 | 2.71 | -9.76 | 9.32 | 8.89 | 13.53 | -0.67 |
Golgi | -1.72 | 6.63 | 1.76 | 2.69 | -2.17 | -2.36 | 5.73 | -0.16 | 1.28 | -2.83 | -2.23 | 9.86 | 1.19 | 2.65 | -3.62 |
Lipid Particles | 1.22 | 6.40 | 7.15 | 2.76 | -0.60 | -1.26 | 0.71 | 3.68 | 4.68 | 1.46 | 0.98 | 5.81 | 7.88 | 6.06 | -0.74 |
Mitochondria | -0.83 | 5.95 | 4.07 | 2.67 | -1.91 | -4.11 | 5.01 | 3.32 | 6.62 | -2.54 | -3.20 | 7.57 | 5.03 | 6.74 | -2.54 |
Mitotic Spindle | -0.83 | 1.30 | -3.30 | -2.27 | -3.89 | -4.18 | -1.03 | -2.19 | 1.63 | -2.02 | -3.52 | 0.18 | -4.03 | -0.82 | -4.04 |
None | 3.66 | -3.98 | 1.33 | -1.61 | 4.59 | -0.63 | -1.26 | 2.36 | 2.71 | 1.34 | -0.46 | -3.68 | 2.48 | 2.41 | 4.43 |
Nuclear Periphery | 1.26 | -6.76 | -4.81 | -6.41 | 2.20 | -2.80 | -8.87 | -6.38 | 0.02 | 7.83 | -1.48 | -9.27 | -6.59 | -3.78 | 5.16 |
Nuclear Periphery Foci | -2.48 | -8.12 | -8.84 | -7.35 | -5.27 | -0.49 | -3.92 | -12.16 | -11.38 | 1.37 | -0.95 | -9.39 | -13.25 | -12.44 | -5.96 |
Nucleolus | -1.35 | 2.41 | 3.42 | 2.62 | 6.88 | -4.42 | -1.13 | 4.65 | 7.52 | 2.61 | -2.76 | 2.35 | 4.69 | 7.40 | 7.83 |
Nucleus | 7.11 | -33.87 | -4.73 | -9.49 | 25.85 | 5.53 | -7.46 | -0.75 | -4.42 | 7.13 | 7.37 | -36.17 | -3.98 | -9.22 | 29.99 |
Peroxisomes | -4.91 | 10.46 | 5.89 | 5.72 | -2.74 | -6.81 | 9.24 | -1.52 | 4.05 | -3.79 | -6.79 | 13.29 | 2.96 | 7.82 | -4.79 |
Vacuole | -6.41 | -3.25 | -25.40 | -12.08 | -23.07 | -12.72 | -7.31 | -19.51 | -13.82 | 2.07 | -12.89 | -8.01 | -31.99 | -23.22 | -23.28 |
Vacuole Periphery | -2.64 | 3.74 | 0.17 | 2.54 | -2.18 | -3.79 | 4.24 | 2.85 | 6.38 | -1.54 | -4.04 | 5.22 | 0.94 | 4.18 | -2.34 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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