Standard name
Human Ortholog
Description NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases Ypk1p and Ypk2p, dephosphorylation increases catalytic activity; forms a heterodimer with Pnc1p to facilitate its peroxisomal import
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0.05 0.05 0.08 0.08 0.11 0.12 0 0.07 0.07 0.06 0.08 0.09 0 – 0.17 0.1 0.11 0.1
Bud – – 0 0 0 0.06 0.08 0.06 0.09 0.1 0.13 0.08 0 0 0 0 0 0.05 0 – 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0.06 0.05 0 0 0.06 0.07 0.07 0.08 0.1 0.05 0.12 0.14 0.07 0.06 0.05 0 – 0.07 0.06 0.1 0.1
Cytoplasm – – 0.93 0.88 0.76 0.5 0.35 0.38 0.3 0.21 0.2 0.22 0.91 0.8 0.72 0.79 0.82 0.78 0 – 0.28 0.53 0.45 0.54
Endoplasmic Reticulum – – 0 0 0 0.06 0.08 0.07 0.1 0.12 0.09 0.13 0 0.06 0.13 0.05 0.09 0.1 0 – 0 0 0.05 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Mitochondria – – 0 0.11 0.2 0.57 0.64 0.5 0.59 0.69 0.67 0.61 0 0 0 0 0.07 0 0.08 – 0.3 0.16 0.12 0.12
Nucleus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0
Vac/Vac Membrane – – 0 0 0 0 0 0.08 0.05 0 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 2 0 8 9 34 29 44 47 74 89 15 30 30 22 25 20 0 – 67 29 28 40
Bud – – 6 6 15 33 54 34 45 56 85 63 4 4 5 7 4 11 0 – 8 4 3 11
Bud Neck – – 10 4 4 2 2 0 2 0 0 1 2 3 3 3 4 3 0 – 11 9 15 11
Bud Site – – 0 2 2 3 2 3 4 7 10 13 0 1 0 0 0 0 – – – – – –
Cell Periphery – – 10 23 21 21 27 36 36 37 56 78 22 51 57 26 19 10 0 – 28 16 26 39
Cytoplasm – – 337 332 344 278 238 225 156 114 138 163 371 344 291 278 261 171 0 – 109 159 122 224
Endoplasmic Reticulum – – 1 2 9 32 54 40 51 65 60 100 11 25 52 19 28 21 0 – 13 12 13 15
Endosome – – 3 0 8 2 3 18 7 2 12 10 1 2 2 4 3 2 0 – 8 2 4 5
Golgi – – 0 0 1 1 0 6 8 6 13 18 2 2 6 1 1 2 0 – 0 1 1 3
Mitochondria – – 12 41 91 320 431 298 312 383 454 463 2 4 3 11 21 6 0 – 114 47 31 48
Nucleus – – 1 1 0 2 3 4 1 2 4 3 0 0 3 6 3 0 0 – 0 1 1 0
Nuclear Periphery – – 0 1 0 2 6 16 12 14 9 19 0 0 0 0 0 1 0 – 1 1 0 1
Nucleolus – – 0 0 1 1 2 3 1 3 5 4 0 0 0 0 0 0 0 – 0 0 0 0
Peroxisomes – – 2 14 12 2 0 17 5 6 10 14 0 4 1 5 0 1 0 – 1 0 0 0
SpindlePole – – 7 11 12 2 12 17 16 9 20 14 2 6 13 13 2 5 0 – 4 1 2 4
Vac/Vac Membrane – – 1 3 6 7 27 46 28 23 30 41 1 4 3 8 2 1 0 – 4 7 9 5
Unique Cell Count 364 376 452 561 677 597 528 555 678 755 406 429 403 353 319 218 1 387 300 271 418
Labelled Cell Count 392 440 534 717 895 792 728 774 980 1093 433 480 469 403 373 254 1 387 300 271 418


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 4.0 4.1 4.9 3.6 4.1 5.0 5.3 6.1 6.7 7.2 5.8 6.7 7.3 5.9 5.7 5.4 5.5 5.7 5.9
Std Deviation (1e-4) – – 1.5 3.3 5.5 2.1 1.9 3.2 3.6 4.6 5.9 6.4 2.1 3.0 3.7 1.4 1.6 1.9 1.5 1.6 1.8
Intensity Change (Log2) – – – 0.04 0.31 -0.13 0.04 0.33 0.43 0.62 0.76 0.87 0.54 0.76 0.88 0.57 0.53 0.44 0.48 0.52 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.7 5.2 5.2 6.1 6.4 3.0 4.6 4.8 4.2 4.9 5.3
Bud 0 1.5 0 0 3.0 4.3 5.0 5.9 4.4 0 0 -0.5 0.3 0 2.4
Bud Neck 0 -2.0 0 0 0 0 0 0 0 -2.5 -2.3 -2.1 -1.9 -1.4 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.4 0 0 2.3 2.7 2.6 3.5 4.4 1.9 4.8 5.6 2.8 2.1 1.2
Cytoplasm -2.0 -6.3 -13.5 -17.8 -16.8 -18.6 -21.4 -22.3 -22.4 -0.6 -5.0 -7.3 -5.3 -4.3 -5.0
Endoplasmic Reticulum 0 0 0 0 4.8 5.9 6.6 5.6 7.1 2.7 4.4 6.9 4.2 5.5 5.7
Endosome 0 0 0 0 2.3 0 0 1.2 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 3.0 0 0 0 0 0 0
Mitochondria 0 7.2 0 0 15.0 17.0 19.7 19.7 18.4 -2.9 -2.4 -2.5 -0.1 2.0 -0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 3.1 0 3.1 0 3.1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 2.3 0 0 2.5 0 0 0 1.7 0 0 0 0 0 0
SpindlePole 0 0.7 0 0 0.9 1.0 -0.3 1.0 -0.1 0 -0.6 1.1 1.4 0 0
Vacuole 0 0 0 3.5 5.2 4.2 3.6 3.8 4.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2799 1.4244 1.3159 1.2886 1.8413 1.6422 1.8073 2.2596 2.4736 2.477 1.9809 2.0146 1.2076 1.2442 0.6223 0.6779 0.7571 0.5248
Actin 0.0437 0.0339 0.0529 0.0171 0.0112 0.0391 0.1085 0.0583 0.1439 0.0611 0.0997 0.0763 0.0901 0.0622 0.0971 0.1239 0.1336 0.0942
Bud 0.0046 0.0081 0.0124 0.0056 0.0021 0.0028 0.0064 0.0113 0.0152 0.017 0.0138 0.0096 0.0058 0.0092 0.0154 0.0179 0.0089 0.004
Bud Neck 0.0082 0.0054 0.0072 0.0026 0.0098 0.0382 0.0117 0.0075 0.0179 0.013 0.0077 0.1221 0.0162 0.0065 0.0088 0.0095 0.0374 0.0928
Bud Periphery 0.0296 0.0156 0.0533 0.0486 0.0329 0.0267 0.0314 0.0323 0.0832 0.1392 0.0728 0.0613 0.0328 0.0228 0.0707 0.1401 0.0419 0.0335
Bud Site 0.0251 0.1489 0.0475 0.012 0.0032 0.0067 0.0402 0.1935 0.081 0.032 0.0204 0.0071 0.0404 0.2586 0.0695 0.0218 0.0104 0.0048
Cell Periphery 0.0497 0.0358 0.0391 0.045 0.1389 0.0711 0.0904 0.0904 0.1221 0.1319 0.0391 0.0987 0.144 0.0724 0.1044 0.1301 0.0866 0.0965
Cytoplasm 0.2104 0.1743 0.1904 0.2805 0.3396 0.2555 0.2888 0.3407 0.2537 0.3061 0.2525 0.4294 0.2756 0.2877 0.2458 0.239 0.2741 0.3375
Cytoplasmic Foci 0.0151 0.011 0.0187 0.0132 0.0229 0.0191 0.0425 0.0122 0.0195 0.0068 0.0367 0.0176 0.0309 0.0063 0.0211 0.0349 0.0168 0.0197
Eisosomes 0.0064 0.0023 0.0038 0.0021 0.0052 0.0026 0.0155 0.0063 0.0096 0.0089 0.0033 0.0059 0.0201 0.0073 0.01 0.0058 0.0047 0.0063
Endoplasmic Reticulum 0.0239 0.0028 0.0047 0.0089 0.0054 0.0068 0.0219 0.0203 0.0115 0.0244 0.0089 0.019 0.0225 0.0063 0.0115 0.013 0.0113 0.0118
Endosome 0.0043 0.0021 0.0047 0.0036 0.0061 0.0089 0.03 0.0049 0.0153 0.0087 0.0625 0.0108 0.026 0.0023 0.0135 0.0064 0.0384 0.0119
Golgi 0.0038 0.0013 0.0041 0.0017 0.0027 0.0055 0.018 0.0064 0.0147 0.0136 0.0315 0.0071 0.0172 0.0033 0.0107 0.0094 0.0292 0.0116
Lipid Particles 0.0043 0.0012 0.0013 0.0014 0.0027 0.0021 0.0166 0.0036 0.0076 0.0058 0.0219 0.0059 0.0177 0.001 0.0115 0.0101 0.0475 0.0105
Mitochondria 0.0033 0.0012 0.0038 0.0039 0.0111 0.0035 0.0214 0.0008 0.0063 0.0156 0.0426 0.0022 0.0064 0.0016 0.0054 0.0148 0.0392 0.007
None 0.5578 0.5378 0.5389 0.5475 0.3941 0.5041 0.2065 0.2046 0.1751 0.2049 0.2472 0.1136 0.2184 0.2479 0.2853 0.2027 0.1797 0.2331
Nuclear Periphery 0.0014 0.0009 0.0007 0.0006 0.0004 0.0006 0.0148 0.0009 0.004 0.0021 0.0062 0.0033 0.0085 0.0004 0.0017 0.001 0.0088 0.0034
Nucleolus 0.0006 0.0006 0.0024 0.0005 0.0003 0.0003 0.0017 0.0004 0.0007 0.0005 0.002 0.0005 0.0009 0.0003 0.0012 0.0003 0.0008 0.0004
Nucleus 0.0012 0.0047 0.0031 0.0013 0.0006 0.0016 0.0054 0.001 0.0016 0.0012 0.0032 0.0018 0.0041 0.0007 0.0014 0.0008 0.0014 0.0043
Peroxisomes 0.001 0.0026 0.0022 0.0012 0.0084 0.0013 0.0084 0.0005 0.0047 0.0021 0.0068 0.0007 0.0092 0.0003 0.0063 0.0146 0.0099 0.0132
Punctate Nuclear 0.0008 0.0036 0.0043 0.0008 0.0004 0.0013 0.0101 0.0005 0.0081 0.0005 0.0057 0.0012 0.0049 0.0002 0.0022 0.0008 0.0074 0.0012
Vacuole 0.0044 0.0057 0.0039 0.0017 0.0018 0.0017 0.0075 0.0035 0.0038 0.0041 0.0129 0.0054 0.0073 0.0025 0.0058 0.0025 0.007 0.0019
Vacuole Periphery 0.0005 0.0002 0.0004 0.0002 0.0004 0.0003 0.0022 0.0002 0.0006 0.0008 0.0026 0.0006 0.001 0.0002 0.0006 0.0004 0.0049 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.4936 52.8416 82.1558 129.0559 60.8253 128.4717 99.8596 125.3526 197.7931 84.452
Translational Efficiency 1.7246 1.5433 1.2882 1.891 1.9835 1.4559 1.6743 1.782 1.7372 1.8498

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘