Standard name
Description Ser/Thr kinase and catalytic subunit of DDK (Dbf4-dependent kinase); required for origin firing and replication initiation; phosphorylates MCM subunits and Cdc45p to drive assembly of the pre-IC and the replicative CMG helicase (Cdc45-MCM-GINS); regulates pre-meiotic DNA replication, meiotic DSB formation, monopolar attachment of homologs during MI by recruiting monopolin to kinetochores, and regulation of middle meiotic genes including NDT80; complemented by co-expression of human CDC7 and DBF4

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0.06 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.33 0.46 0.27 0.26 0.38 0.34 0.32 0.31 0.33 0.36 0.37 0.34 0.35 0.4 0.4 0.3 0.35 0.29 0.2 0.32 0.62 0.57 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.05 0.16 0.36 0.4 0.48 0.59 0.54 0.66 0.66 0.66 0.69 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.34 0.33 0.33 0.45 0.43 0.34 0.29 0.37 0.28 0.27 0.27 0.2 0.44 0.47 0.46 0.34 0.44 0.34 0.42 0.49 0.34 0.17 0.16 0.09
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0.05 0
Vac/Vac Membrane 0.29 0.38 0.18 0.07 0.1 0.1 0.07 0.06 0.08 0.07 0 0 0.28 0.2 0.19 0.32 0.38 0.43 0.08 0.11 0.11 0.08 0.09 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 1 0 1 0 2 5
Bud 0 0 1 1 1 2 1 2 5 7 3 2 1 0 0 0 0 1 29 16 9 7 7 7
Bud Neck 0 0 0 0 1 0 0 1 0 1 1 0 0 0 0 0 0 0 1 1 1 3 5 12
Bud Site 0 0 0 1 1 2 0 7 0 2 3 2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 1 0 2 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 106 79 70 45 47 70 68 81 72 94 62 84 105 157 153 172 114 96 86 53 56 161 188 177
Endoplasmic Reticulum 2 0 1 1 2 1 0 0 0 0 0 0 0 2 0 14 16 10 0 1 1 0 5 6
Endosome 1 5 0 0 0 0 2 1 0 0 0 0 3 10 4 8 8 2 6 4 3 7 5 7
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 1 2 0 0 0 2
Mitochondria 31 12 25 60 73 89 119 137 151 189 113 157 6 5 11 12 4 4 3 4 5 1 6 6
Nucleus 93 80 51 75 78 62 58 93 65 77 47 45 133 210 179 146 166 93 126 132 60 43 52 31
Nuclear Periphery 1 0 0 0 1 0 0 0 2 1 0 0 0 2 1 0 0 0 1 0 1 0 1 2
Nucleolus 2 0 0 0 1 2 1 3 0 1 1 3 0 3 7 2 0 3 2 1 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 1 2 0 0 1 1 0 3 1 0 0 0 15 15 10 6 17 13
Vac/Vac Membrane 81 92 27 11 18 19 14 16 19 20 4 10 84 91 75 138 145 119 24 30 19 21 30 56
Unique Cell Count 276 239 153 167 183 184 200 252 229 287 172 228 305 446 386 430 379 276 303 270 177 262 332 343
Labelled Cell Count 317 268 175 196 224 247 264 343 315 393 235 306 334 488 433 493 454 328 303 270 177 262 332 343


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.5 4.6 4.2 4.2 3.9 3.4 3.4 3.5 3.2 3.6 3.0 6.3 6.3 6.2 6.5 8.1 8.1 5.1 5.1 6.1
Std Deviation (1e-4) 0.7 0.9 1.1 1.2 1.1 1.4 1.3 0.8 1.3 1.1 1.7 1.3 1.4 1.7 1.7 1.4 1.6 1.6 1.2 1.3 1.4
Intensity Change (Log2) -0.11 -0.13 -0.21 -0.43 -0.42 -0.37 -0.52 -0.35 -0.58 0.47 0.46 0.44 0.52 0.83 0.83 0.16 0.17 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.5 -3.8 -1.4 -2.2 -2.7 -2.8 -2.7 -1.8 -1.7 -2.4 -2.3 -1.3 -1.2 -3.4 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.8 -7.4 -5.7 -5.9 -7.0 -5.9
Nucleus 2.1 1.7 0.1 -0.9 0.7 -1.0 -1.4 -1.2 -3.0 2.1 3.0 2.8 0.1 2.2 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.1 -2.1 -1.9 -3.1 -3.6 -2.8 -3.5 -4.7 -4.3 2.3 0.7 0.5 3.4 4.6 5.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available