Standard name
Description Ser/Thr kinase and catalytic subunit of DDK (Dbf4-dependent kinase); required for origin firing and replication initiation; phosphorylates MCM subunits and Cdc45p to drive assembly of the pre-IC and the replicative CMG helicase (Cdc45-MCM-GINS); regulates pre-meiotic DNA replication, meiotic DSB formation, monopolar attachment of homologs during MI by recruiting monopolin to kinetochores, and regulation of middle meiotic genes including NDT80; complemented by co-expression of human CDC7 and DBF4

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0.06 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.38 0.33 0.46 0.27 0.26 0.38 0.34 0.32 0.31 0.33 0.36 0.37 0.34 0.35 0.4 0.4 0.3 0.35 0.29 0.2 0.32 0.62 0.57 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.05 0.16 0.36 0.4 0.48 0.59 0.54 0.66 0.66 0.66 0.69 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.34 0.33 0.33 0.45 0.43 0.34 0.29 0.37 0.28 0.27 0.27 0.2 0.44 0.47 0.46 0.34 0.44 0.34 0.42 0.49 0.34 0.17 0.16 0.09
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0.05 0
Vac/Vac Membrane 0.29 0.38 0.18 0.07 0.1 0.1 0.07 0.06 0.08 0.07 0 0 0.28 0.2 0.19 0.32 0.38 0.43 0.08 0.11 0.11 0.08 0.09 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 1 0 1 0 2 5
Bud 0 0 1 1 1 2 1 2 5 7 3 2 1 0 0 0 0 1 29 16 9 7 7 7
Bud Neck 0 0 0 0 1 0 0 1 0 1 1 0 0 0 0 0 0 0 1 1 1 3 5 12
Bud Site 0 0 0 1 1 2 0 7 0 2 3 2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 1 0 2 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 106 79 70 45 47 70 68 81 72 94 62 84 105 157 153 172 114 96 86 53 56 161 188 177
Endoplasmic Reticulum 2 0 1 1 2 1 0 0 0 0 0 0 0 2 0 14 16 10 0 1 1 0 5 6
Endosome 1 5 0 0 0 0 2 1 0 0 0 0 3 10 4 8 8 2 6 4 3 7 5 7
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 0 0 0 1 2 0 0 0 2
Mitochondria 31 12 25 60 73 89 119 137 151 189 113 157 6 5 11 12 4 4 3 4 5 1 6 6
Nucleus 93 80 51 75 78 62 58 93 65 77 47 45 133 210 179 146 166 93 126 132 60 43 52 31
Nuclear Periphery 1 0 0 0 1 0 0 0 2 1 0 0 0 2 1 0 0 0 1 0 1 0 1 2
Nucleolus 2 0 0 0 1 2 1 3 0 1 1 3 0 3 7 2 0 3 2 1 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 1 2 0 0 1 1 0 3 1 0 0 0 15 15 10 6 17 13
Vac/Vac Membrane 81 92 27 11 18 19 14 16 19 20 4 10 84 91 75 138 145 119 24 30 19 21 30 56
Unique Cell Count 276 239 153 167 183 184 200 252 229 287 172 228 305 446 386 430 379 276 303 270 177 262 332 343
Labelled Cell Count 317 268 175 196 224 247 264 343 315 393 235 306 334 488 433 493 454 328 303 270 177 262 332 343


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.5 4.6 4.2 4.2 3.9 3.4 3.4 3.5 3.2 3.6 3.0 6.3 6.3 6.2 6.5 8.1 8.1 5.1 5.1 6.1
Std Deviation (1e-4) 0.7 0.9 1.1 1.2 1.1 1.4 1.3 0.8 1.3 1.1 1.7 1.3 1.4 1.7 1.7 1.4 1.6 1.6 1.2 1.3 1.4
Intensity Change (Log2) -0.11 -0.13 -0.21 -0.43 -0.42 -0.37 -0.52 -0.35 -0.58 0.47 0.46 0.44 0.52 0.83 0.83 0.16 0.17 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.5 -3.8 -1.4 -2.2 -2.7 -2.8 -2.7 -1.8 -1.7 -2.4 -2.3 -1.3 -1.2 -3.4 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.8 -7.4 -5.7 -5.9 -7.0 -5.9
Nucleus 2.1 1.7 0.1 -0.9 0.7 -1.0 -1.4 -1.2 -3.0 2.1 3.0 2.8 0.1 2.2 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.1 -2.1 -1.9 -3.1 -3.6 -2.8 -3.5 -4.7 -4.3 2.3 0.7 0.5 3.4 4.6 5.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5885 5.0109 5.3095 4.8041 4.1028 5.0649 1.9952 2.6985 2.9127 2.4988 2.7196 2.4742 4.1613 3.8372 3.5361 4.2598 4.1132 3.1836
Actin 0.0287 0.0161 0.0253 0.0001 0.0044 0.0028 0.0223 0.0013 0.007 0.0007 0.0307 0.0056 0.0878 0.0003 0.0244 0.0353 0.0007 0.0006
Bud 0.0015 0.0023 0.0007 0 0.0002 0.0011 0.0014 0.0002 0.0001 0 0.0004 0.0003 0.0024 0.0002 0.0005 0.0015 0.0001 0.0002
Bud Neck 0.0024 0.0009 0.0005 0.0002 0.004 0.0107 0.003 0.0007 0.0009 0.0004 0.0012 0.0038 0.027 0.0008 0.002 0.0038 0.0027 0.0032
Bud Periphery 0.0016 0.002 0.0007 0 0.0002 0.0017 0.0022 0.0001 0.0001 0.0001 0.0004 0.0003 0.0041 0.0001 0.0007 0.0015 0.0001 0.0001
Bud Site 0.0039 0.0068 0.0095 0.0001 0.0005 0.0007 0.0054 0.0024 0.0017 0.0001 0.0009 0.0004 0.0081 0.002 0.0152 0.0108 0.001 0.0006
Cell Periphery 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0004 0.0001 0 0 0.0001 0.0001 0.0009 0.0001 0.0004 0.0001 0.0001 0
Cytoplasm 0.2833 0.2119 0.028 0.0138 0.164 0.2704 0.2576 0.129 0.0279 0.0148 0.1708 0.2356 0.3019 0.2896 0.0516 0.0222 0.3172 0.3543
Cytoplasmic Foci 0.0262 0.0034 0.002 0.0005 0.0128 0.0068 0.0165 0.0034 0.0084 0.0005 0.0133 0.0085 0.0203 0.0029 0.001 0.0177 0.0028 0.0075
Eisosomes 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0007 0 0.0002 0 0.0002 0 0.0005 0 0.0004 0.0001 0 0
Endoplasmic Reticulum 0.0137 0.0056 0.0016 0.0007 0.0103 0.0054 0.0102 0.0035 0.0013 0.0011 0.0073 0.0038 0.0581 0.0029 0.0008 0.0005 0.0044 0.0044
Endosome 0.047 0.0042 0.0054 0.001 0.0139 0.0074 0.0211 0.005 0.0056 0.0015 0.0189 0.0074 0.0505 0.0009 0.001 0.0076 0.0036 0.004
Golgi 0.0064 0.0012 0.0025 0 0.0023 0.0016 0.0062 0.0004 0.0021 0 0.0058 0.0008 0.0182 0.0001 0.0006 0.0117 0.0001 0.0011
Lipid Particles 0.0085 0.0005 0.0049 0 0.0056 0.0031 0.01 0.0004 0.0017 0.0001 0.0144 0.001 0.0318 0.0001 0.0005 0.0021 0.0003 0.0113
Mitochondria 0.013 0.0022 0.0026 0.0002 0.0009 0.0016 0.0072 0.0007 0.0011 0.0011 0.0024 0.0028 0.0048 0.0003 0.0041 0.0017 0.0006 0.0018
None 0.0903 0.0567 0.0105 0.0035 0.0179 0.1139 0.1937 0.0737 0.033 0.0136 0.0896 0.1251 0.0918 0.1377 0.006 0.1042 0.0665 0.0557
Nuclear Periphery 0.0526 0.0164 0.0102 0.0222 0.0181 0.0132 0.0313 0.0135 0.013 0.0301 0.0346 0.0143 0.0463 0.0065 0.0069 0.0038 0.0253 0.0138
Nucleolus 0.0043 0.004 0.0034 0.0018 0.0032 0.0031 0.005 0.0026 0.0034 0.0038 0.0046 0.0041 0.0042 0.0018 0.0024 0.001 0.0043 0.0029
Nucleus 0.3566 0.6479 0.8829 0.9485 0.7198 0.5323 0.3615 0.745 0.8748 0.924 0.561 0.5513 0.1914 0.5399 0.8768 0.7598 0.4745 0.4954
Peroxisomes 0.0171 0.0013 0.0023 0 0.001 0.0016 0.0119 0.0003 0.0061 0.0001 0.011 0.0027 0.0114 0.0001 0.0014 0.0121 0.0001 0.0021
Punctate Nuclear 0.0278 0.0101 0.0054 0.0068 0.0176 0.0181 0.0213 0.015 0.0103 0.0074 0.0275 0.0285 0.025 0.0117 0.0022 0.002 0.0929 0.0392
Vacuole 0.0108 0.0057 0.0013 0.0003 0.0026 0.0034 0.0088 0.0024 0.0009 0.0004 0.0039 0.0031 0.0106 0.0017 0.0009 0.0004 0.0022 0.0016
Vacuole Periphery 0.0036 0.0006 0.0003 0.0002 0.0005 0.0009 0.0023 0.0003 0.0003 0.0004 0.0012 0.0005 0.003 0.0002 0.0002 0.0001 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.2223 11.0029 8.1646 8.7163 7.0404 5.0653 6.5441 6.6253 7.0319 10.8934
Translational Efficiency 1.0654 0.8436 0.8764 1.0704 1.3681 2.5106 1.3834 1.2891 1.27 1.0746

Hit Data

Dataset Hit
Protein Abundance
Protein Localization
Gene Expression
Translational Efficiency