ORF
Description Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.3 0.23 0.21 0.11 0.26 0.14 – 0.07 0.08 0.15 0.1 0.12 0.2 0.23 0.14 0.27 0.25 0.26 0.06 0.06 0 0.08 0.08 0.07
Bud 0.08 0 0.1 0.06 0.06 0.05 – 0.08 0.06 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0.08 0.08 0.09 0.1
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.5 0.47 0.42 0.56 0.44 0.41 – 0.27 0.36 0.36 0.45 0.41 0.65 0.63 0.7 0.62 0.63 0.69 0.68 0.65 0.71 0.62 0.66 0.65
Cytoplasm 0.4 0.51 0.48 0.28 0.34 0.41 – 0.45 0.42 0.36 0.35 0.32 0.5 0.48 0.47 0.16 0.17 0.17 0.05 0.06 0.06 0 0.06 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.05 0.27 0.61 0.24 0.44 – 0.46 0.51 0.51 0.59 0.61 0.09 0.07 0.08 0.12 0.1 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.06 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 121 82 50 33 94 57 – 49 32 70 42 55 44 77 52 32 35 37 31 28 11 11 22 19
Bud 33 12 23 16 21 22 – 51 26 27 24 26 9 11 6 4 5 6 8 4 3 0 3 4
Bud Neck 10 11 5 0 3 0 – 1 0 0 0 0 0 0 0 0 1 0 60 44 22 11 26 27
Bud Site 10 7 4 3 6 2 – 5 1 2 2 1 0 1 1 1 0 1 – – – – – –
Cell Periphery 203 169 102 161 158 164 – 176 151 174 180 181 145 212 262 74 89 98 381 287 194 88 195 184
Cytoplasm 162 180 116 80 121 165 – 296 173 174 141 142 111 161 178 19 24 24 29 25 15 6 17 15
Endoplasmic Reticulum 2 10 3 1 11 14 – 5 8 8 6 6 1 1 5 0 5 6 9 12 9 4 13 10
Endosome 1 0 0 2 4 2 – 2 0 1 1 0 0 0 1 1 0 0 2 3 1 1 2 2
Golgi 0 1 0 0 1 0 – 0 0 0 0 0 0 0 0 2 5 3 0 2 0 0 1 0
Mitochondria 29 18 65 176 86 176 – 308 213 247 239 272 19 25 30 14 14 14 14 11 9 4 6 6
Nucleus 3 1 1 0 1 1 – 2 0 1 1 0 0 0 0 1 2 4 0 0 0 1 0 0
Nuclear Periphery 0 0 0 1 0 0 – 3 1 1 1 0 0 0 0 0 0 0 0 2 0 0 0 0
Nucleolus 0 0 1 0 1 0 – 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 2 1 2 13 23 18 – 22 8 13 8 11 7 13 20 3 3 0 0 0 0 0 0 0
SpindlePole 10 13 5 3 7 3 – 11 6 3 3 5 3 6 6 7 3 6 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 2 5 – 21 14 1 4 6 0 1 3 1 0 4 19 13 1 8 3 5
Unique Cell Count 407 356 240 287 356 402 663 414 482 403 446 223 337 375 120 141 143 562 440 276 143 297 283
Labelled Cell Count 586 505 378 490 539 629 953 633 722 652 705 339 508 565 159 186 203 562 440 276 143 297 283


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 6.1 5.0 5.6 6.0 5.2 – 5.2 4.9 5.1 5.1 5.1 7.1 7.0 6.9 11.1 10.8 10.3 6.3 7.3 7.1
Std Deviation (1e-4) 0.7 0.7 1.6 1.7 1.3 1.9 – 1.3 1.1 0.9 1.3 1.2 1.5 1.3 1.4 2.0 2.1 1.9 1.5 1.8 1.8
Intensity Change (Log2) – – – 0.17 0.26 0.08 – 0.08 -0.03 0.04 0.03 0.03 0.51 0.5 0.47 1.16 1.12 1.06 0.35 0.56 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.9 1.6 -2.2 – -5.7 0 -2.1 -3.6 -2.9 -0.3 0.6 -2.3 1.2 0.9 1.1
Bud -1.8 -1.7 -2.0 – -0.9 0 -2.0 -1.7 -1.8 -2.4 -3.2 -4.6 -2.1 -2.2 -1.9
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.1 0.5 -0.4 – -4.6 0 -1.7 0.5 -0.5 4.9 4.9 6.7 3.4 3.9 4.9
Cytoplasm -4.8 -3.5 -1.8 – -1.0 -1.6 -3.2 -3.3 -4.3 0.3 -0.1 -0.2 -6.0 -6.1 -6.2
Endoplasmic Reticulum 0 1.5 1.7 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7.9 -0.8 4.2 – 5.2 0 6.2 7.9 8.5 -5.2 -6.4 -6.4 -3.3 -4.0 -4.0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 2.5 3.4 2.6 – 2.1 0 0 0 0 0 2.3 2.9 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 2.4 2.4 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9638 3.9584 3.6156 3.3888 2.995 3.7121 3.3494 3.6402 3.2561 2.8984 3.1781 3.3064 5.6603 5.1634 4.7356 4.6553 4.5173 5.0617
Actin 0.0103 0.0015 0.0073 0.0514 0.0417 0.018 0.0626 0.0028 0.0162 0.0182 0.0066 0.0009 0.0368 0.0009 0.0131 0.0091 0.0041 0.0018
Bud 0.0003 0.0005 0.0008 0.0009 0.0006 0.0005 0.0072 0.0009 0.0016 0.0008 0.0037 0.0017 0.0007 0.0007 0.0011 0.0009 0.0002 0.0005
Bud Neck 0.0017 0.0055 0.0146 0.0101 0.01 0.062 0.0081 0.0082 0.0058 0.0092 0.0038 0.0489 0.0034 0.0066 0.006 0.0009 0.0004 0.0501
Bud Periphery 0.0024 0.0028 0.0125 0.012 0.0084 0.0083 0.0152 0.0048 0.0141 0.0083 0.0139 0.0098 0.0046 0.005 0.015 0.0276 0.0027 0.0092
Bud Site 0.0116 0.1437 0.0387 0.0075 0.0038 0.0026 0.0273 0.1678 0.04 0.0054 0.0193 0.0016 0.0112 0.1795 0.0361 0.0008 0.0005 0.002
Cell Periphery 0.8156 0.7588 0.7832 0.7569 0.8705 0.8318 0.7043 0.6994 0.7977 0.8265 0.745 0.8721 0.8048 0.6487 0.7877 0.848 0.7735 0.8616
Cytoplasm 0.0694 0.0808 0.0933 0.0779 0.045 0.0549 0.0531 0.0983 0.082 0.0603 0.0476 0.0517 0.0586 0.1404 0.0997 0.0599 0.0719 0.0533
Cytoplasmic Foci 0.0125 0.0001 0.0054 0.0096 0.0018 0.0011 0.0107 0.0007 0.0019 0.0011 0.0245 0.0002 0.0104 0.0027 0.008 0.0124 0.0027 0.0003
Eisosomes 0.0077 0.0021 0.0023 0.0015 0.0008 0.0011 0.0185 0.0024 0.0009 0.0025 0.0008 0.0006 0.0034 0.0008 0.0016 0.0003 0.0003 0.0007
Endoplasmic Reticulum 0.0131 0.0011 0.0035 0.005 0.0057 0.0017 0.0139 0.0053 0.0073 0.0166 0.0168 0.0027 0.009 0.0013 0.0085 0.0039 0.0024 0.0023
Endosome 0.0128 0 0.0068 0.0126 0.0018 0.0013 0.0117 0.0001 0.0027 0.0075 0.021 0.0003 0.0072 0.0004 0.001 0.0011 0.0122 0.0003
Golgi 0.0049 0.0001 0.0018 0.0085 0.0037 0.0028 0.0124 0.0003 0.0032 0.0068 0.0062 0.0004 0.0051 0.0003 0.0011 0.0015 0.0024 0.0011
Lipid Particles 0.0124 0.0001 0.0122 0.0133 0.0006 0.0026 0.0087 0.0042 0.0021 0.0038 0.0156 0.0003 0.0074 0.0013 0.0017 0.0025 0.017 0.0018
Mitochondria 0.0021 0 0.0013 0.0062 0.0003 0.005 0.0149 0.0003 0.0046 0.0043 0.013 0.0045 0.0014 0 0.0006 0.0002 0.0432 0.0056
None 0.0024 0.0002 0.004 0.0012 0.0006 0.0011 0.0035 0.0006 0.0072 0.0198 0.0202 0.0006 0.0169 0.0016 0.0019 0.0006 0.0044 0.0029
Nuclear Periphery 0.0033 0.0002 0.0003 0.0004 0.0003 0.0002 0.0038 0.0004 0.0025 0.0016 0.0044 0.0001 0.0036 0.0003 0.0006 0.0004 0.0213 0.0001
Nucleolus 0.0004 0 0.0002 0.0006 0 0.0001 0.0011 0.0001 0.0003 0.0004 0.0033 0.0001 0.0003 0.0001 0.0002 0.0001 0.0009 0.0001
Nucleus 0.0004 0.0002 0.0002 0.0003 0.0001 0.0002 0.002 0.0003 0.0006 0.0009 0.0046 0.0002 0.0009 0.0002 0.0004 0.0001 0.0138 0.0002
Peroxisomes 0.0101 0 0.007 0.0182 0.0002 0.0015 0.0056 0 0.0028 0.0001 0.0049 0 0.0052 0.0012 0.0008 0.0158 0.0069 0.0016
Punctate Nuclear 0.0015 0 0.0003 0.0004 0.0001 0.0002 0.0007 0 0.0002 0.0003 0.0133 0 0.0043 0.0055 0.011 0.0089 0.0138 0
Vacuole 0.0048 0.0023 0.0042 0.0048 0.0039 0.003 0.0133 0.003 0.0058 0.0049 0.0095 0.0033 0.0045 0.0026 0.0038 0.0049 0.0042 0.0041
Vacuole Periphery 0.0005 0 0.0002 0.0008 0 0.0002 0.0013 0.0001 0.0003 0.0008 0.0019 0.0001 0.0003 0 0.0001 0.0001 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.6501 51.7543 47.4401 61.804 61.8166 44.2479 48.481 66.4816 76.997 54.1361
Translational Efficiency 2.4934 2.5984 2.4031 1.7494 1.7149 2.0967 2.678 1.7545 1.3762 2.1904

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘