ORF
Human Ortholog
Description Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.3 0.23 0.21 0.11 0.26 0.14 0.07 0.08 0.15 0.1 0.12 0.2 0.23 0.14 0.27 0.25 0.26 0.06 0.06 0 0.08 0.08 0.07
Bud 0.08 0 0.1 0.06 0.06 0.05 0.08 0.06 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0.08 0.08 0.09 0.1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.5 0.47 0.42 0.56 0.44 0.41 0.27 0.36 0.36 0.45 0.41 0.65 0.63 0.7 0.62 0.63 0.69 0.68 0.65 0.71 0.62 0.66 0.65
Cytoplasm 0.4 0.51 0.48 0.28 0.34 0.41 0.45 0.42 0.36 0.35 0.32 0.5 0.48 0.47 0.16 0.17 0.17 0.05 0.06 0.06 0 0.06 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.05 0.27 0.61 0.24 0.44 0.46 0.51 0.51 0.59 0.61 0.09 0.07 0.08 0.12 0.1 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 121 82 50 33 94 57 49 32 70 42 55 44 77 52 32 35 37 31 28 11 11 22 19
Bud 33 12 23 16 21 22 51 26 27 24 26 9 11 6 4 5 6 8 4 3 0 3 4
Bud Neck 10 11 5 0 3 0 1 0 0 0 0 0 0 0 0 1 0 60 44 22 11 26 27
Bud Site 10 7 4 3 6 2 5 1 2 2 1 0 1 1 1 0 1
Cell Periphery 203 169 102 161 158 164 176 151 174 180 181 145 212 262 74 89 98 381 287 194 88 195 184
Cytoplasm 162 180 116 80 121 165 296 173 174 141 142 111 161 178 19 24 24 29 25 15 6 17 15
Endoplasmic Reticulum 2 10 3 1 11 14 5 8 8 6 6 1 1 5 0 5 6 9 12 9 4 13 10
Endosome 1 0 0 2 4 2 2 0 1 1 0 0 0 1 1 0 0 2 3 1 1 2 2
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 0 2 5 3 0 2 0 0 1 0
Mitochondria 29 18 65 176 86 176 308 213 247 239 272 19 25 30 14 14 14 14 11 9 4 6 6
Nucleus 3 1 1 0 1 1 2 0 1 1 0 0 0 0 1 2 4 0 0 0 1 0 0
Nuclear Periphery 0 0 0 1 0 0 3 1 1 1 0 0 0 0 0 0 0 0 2 0 0 0 0
Nucleolus 0 0 1 0 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 2 1 2 13 23 18 22 8 13 8 11 7 13 20 3 3 0 0 0 0 0 0 0
SpindlePole 10 13 5 3 7 3 11 6 3 3 5 3 6 6 7 3 6 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 2 5 21 14 1 4 6 0 1 3 1 0 4 19 13 1 8 3 5
Unique Cell Count 407 356 240 287 356 402 663 414 482 403 446 223 337 375 120 141 143 562 440 276 143 297 283
Labelled Cell Count 586 505 378 490 539 629 953 633 722 652 705 339 508 565 159 186 203 562 440 276 143 297 283


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 6.1 5.0 5.6 6.0 5.2 5.2 4.9 5.1 5.1 5.1 7.1 7.0 6.9 11.1 10.8 10.3 6.3 7.3 7.1
Std Deviation (1e-4) 0.7 0.7 1.6 1.7 1.3 1.9 1.3 1.1 0.9 1.3 1.2 1.5 1.3 1.4 2.0 2.1 1.9 1.5 1.8 1.8
Intensity Change (Log2) 0.17 0.26 0.08 0.08 -0.03 0.04 0.03 0.03 0.51 0.5 0.47 1.16 1.12 1.06 0.35 0.56 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.9 1.6 -2.2 -5.7 0 -2.1 -3.6 -2.9 -0.3 0.6 -2.3 1.2 0.9 1.1
Bud -1.8 -1.7 -2.0 -0.9 0 -2.0 -1.7 -1.8 -2.4 -3.2 -4.6 -2.1 -2.2 -1.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.1 0.5 -0.4 -4.6 0 -1.7 0.5 -0.5 4.9 4.9 6.7 3.4 3.9 4.9
Cytoplasm -4.8 -3.5 -1.8 -1.0 -1.6 -3.2 -3.3 -4.3 0.3 -0.1 -0.2 -6.0 -6.1 -6.2
Endoplasmic Reticulum 0 1.5 1.7 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 7.9 -0.8 4.2 5.2 0 6.2 7.9 8.5 -5.2 -6.4 -6.4 -3.3 -4.0 -4.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 2.5 3.4 2.6 2.1 0 0 0 0 0 2.3 2.9 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.4 2.4 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9638 3.9584 3.6156 3.3888 2.995 3.7121 3.3494 3.6402 3.2561 2.8984 3.1781 3.3064 5.6603 5.1634 4.7356 4.6553 4.5173 5.0617
Actin 0.0103 0.0015 0.0073 0.0514 0.0417 0.018 0.0626 0.0028 0.0162 0.0182 0.0066 0.0009 0.0368 0.0009 0.0131 0.0091 0.0041 0.0018
Bud 0.0003 0.0005 0.0008 0.0009 0.0006 0.0005 0.0072 0.0009 0.0016 0.0008 0.0037 0.0017 0.0007 0.0007 0.0011 0.0009 0.0002 0.0005
Bud Neck 0.0017 0.0055 0.0146 0.0101 0.01 0.062 0.0081 0.0082 0.0058 0.0092 0.0038 0.0489 0.0034 0.0066 0.006 0.0009 0.0004 0.0501
Bud Periphery 0.0024 0.0028 0.0125 0.012 0.0084 0.0083 0.0152 0.0048 0.0141 0.0083 0.0139 0.0098 0.0046 0.005 0.015 0.0276 0.0027 0.0092
Bud Site 0.0116 0.1437 0.0387 0.0075 0.0038 0.0026 0.0273 0.1678 0.04 0.0054 0.0193 0.0016 0.0112 0.1795 0.0361 0.0008 0.0005 0.002
Cell Periphery 0.8156 0.7588 0.7832 0.7569 0.8705 0.8318 0.7043 0.6994 0.7977 0.8265 0.745 0.8721 0.8048 0.6487 0.7877 0.848 0.7735 0.8616
Cytoplasm 0.0694 0.0808 0.0933 0.0779 0.045 0.0549 0.0531 0.0983 0.082 0.0603 0.0476 0.0517 0.0586 0.1404 0.0997 0.0599 0.0719 0.0533
Cytoplasmic Foci 0.0125 0.0001 0.0054 0.0096 0.0018 0.0011 0.0107 0.0007 0.0019 0.0011 0.0245 0.0002 0.0104 0.0027 0.008 0.0124 0.0027 0.0003
Eisosomes 0.0077 0.0021 0.0023 0.0015 0.0008 0.0011 0.0185 0.0024 0.0009 0.0025 0.0008 0.0006 0.0034 0.0008 0.0016 0.0003 0.0003 0.0007
Endoplasmic Reticulum 0.0131 0.0011 0.0035 0.005 0.0057 0.0017 0.0139 0.0053 0.0073 0.0166 0.0168 0.0027 0.009 0.0013 0.0085 0.0039 0.0024 0.0023
Endosome 0.0128 0 0.0068 0.0126 0.0018 0.0013 0.0117 0.0001 0.0027 0.0075 0.021 0.0003 0.0072 0.0004 0.001 0.0011 0.0122 0.0003
Golgi 0.0049 0.0001 0.0018 0.0085 0.0037 0.0028 0.0124 0.0003 0.0032 0.0068 0.0062 0.0004 0.0051 0.0003 0.0011 0.0015 0.0024 0.0011
Lipid Particles 0.0124 0.0001 0.0122 0.0133 0.0006 0.0026 0.0087 0.0042 0.0021 0.0038 0.0156 0.0003 0.0074 0.0013 0.0017 0.0025 0.017 0.0018
Mitochondria 0.0021 0 0.0013 0.0062 0.0003 0.005 0.0149 0.0003 0.0046 0.0043 0.013 0.0045 0.0014 0 0.0006 0.0002 0.0432 0.0056
None 0.0024 0.0002 0.004 0.0012 0.0006 0.0011 0.0035 0.0006 0.0072 0.0198 0.0202 0.0006 0.0169 0.0016 0.0019 0.0006 0.0044 0.0029
Nuclear Periphery 0.0033 0.0002 0.0003 0.0004 0.0003 0.0002 0.0038 0.0004 0.0025 0.0016 0.0044 0.0001 0.0036 0.0003 0.0006 0.0004 0.0213 0.0001
Nucleolus 0.0004 0 0.0002 0.0006 0 0.0001 0.0011 0.0001 0.0003 0.0004 0.0033 0.0001 0.0003 0.0001 0.0002 0.0001 0.0009 0.0001
Nucleus 0.0004 0.0002 0.0002 0.0003 0.0001 0.0002 0.002 0.0003 0.0006 0.0009 0.0046 0.0002 0.0009 0.0002 0.0004 0.0001 0.0138 0.0002
Peroxisomes 0.0101 0 0.007 0.0182 0.0002 0.0015 0.0056 0 0.0028 0.0001 0.0049 0 0.0052 0.0012 0.0008 0.0158 0.0069 0.0016
Punctate Nuclear 0.0015 0 0.0003 0.0004 0.0001 0.0002 0.0007 0 0.0002 0.0003 0.0133 0 0.0043 0.0055 0.011 0.0089 0.0138 0
Vacuole 0.0048 0.0023 0.0042 0.0048 0.0039 0.003 0.0133 0.003 0.0058 0.0049 0.0095 0.0033 0.0045 0.0026 0.0038 0.0049 0.0042 0.0041
Vacuole Periphery 0.0005 0 0.0002 0.0008 0 0.0002 0.0013 0.0001 0.0003 0.0008 0.0019 0.0001 0.0003 0 0.0001 0.0001 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.6501 51.7543 47.4401 61.804 61.8166 44.2479 48.481 66.4816 76.997 54.1361
Translational Efficiency 2.4934 2.5984 2.4031 1.7494 1.7149 2.0967 2.678 1.7545 1.3762 2.1904

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1669 1673 338 457 834 1634 2588 1676 2503 3307 2926 2133

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 723.44 859.72 1078.77 1052.76 713.29 808.89 1046.78 1001.12 720.06 834.60 1050.48 1012.18
Standard Deviation 104.69 111.60 115.38 131.22 94.90 112.06 115.05 120.88 101.64 114.68 115.55 124.98
Intensity Change Log 2 0.248993 0.576442 0.541231 0.181455 0.553398 0.489054 0.215858 0.565047 0.515563

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006987 0.012622 0.035134 0.065609 0.005099 0.010124 0.018178 0.057067 0.006358 0.011388 0.020137 0.058897
Bud Neck 0.103951 0.086780 0.060935 0.076224 0.047472 0.088029 0.107143 0.085103 0.085132 0.087398 0.101805 0.083201
Bud Site 0.059920 0.087323 0.184053* 0.220744* 0.043836 0.086254 0.103807* 0.210767* 0.054561 0.086795 0.113076* 0.212905*
Cell Periphery 0.105524* 0.239366* 0.181421* 0.345328* 0.349268* 0.187248* 0.404758* 0.303720* 0.186739* 0.213614* 0.378959* 0.312634*
Cytoplasm 0.525907* 0.499205* 0.216872* 0.160190* 0.460526* 0.512000* 0.281262* 0.255961* 0.504122* 0.505527* 0.273824* 0.235442*
Cytoplasmic Foci 0.054037 0.009756 0.024086 0.006315 0.010590 0.024592 0.001887 0.003709 0.039560 0.017086 0.004451 0.004267
Eisosomes 0.027854 0.022034 0.022492 0.040328 0.022768 0.026345 0.024700 0.028685 0.026159 0.024164 0.024445 0.031180
Endoplasmic Reticulum 0.007411 0.004603 0.110033* 0.017192 0.010836 0.004572 0.013601 0.005508 0.008552 0.004587 0.024741 0.008011
Endosome 0.012696 0.002636 0.004110 0.001800 0.002654 0.005685 0.000765 0.002101 0.009350 0.004142 0.001152 0.002036
Golgi 0.019283 0.005541 0.004341 0.002848 0.005491 0.011633 0.000850 0.001848 0.014688 0.008551 0.001253 0.002062
Lipid Particles 0.025035 0.002715 0.009277 0.002714 0.003668 0.006542 0.002114 0.001489 0.017915 0.004606 0.002941 0.001751
Mitochondria 0.008060 0.004053 0.000445 0.000449 0.007648 0.006719 0.000842 0.001648 0.007923 0.005370 0.000797 0.001391
Mitotic Spindle 0.000081 0.000191 0.000230 0.000298 0.000095 0.000309 0.000321 0.001532 0.000086 0.000249 0.000311 0.001268
None 0.000834 0.000124 0.000134 0.000048 0.001284 0.000351 0.000212 0.000100 0.000984 0.000237 0.000203 0.000089
Nuclear Periphery 0.000141 0.000065 0.000221 0.000092 0.000142 0.000106 0.000240 0.000064 0.000141 0.000085 0.000238 0.000070
Nuclear Periphery Foci 0.000334 0.000217 0.006517 0.000909 0.000329 0.000284 0.000601 0.000332 0.000332 0.000250 0.001285 0.000456
Nucleolus 0.000286 0.000055 0.000053 0.000015 0.000937 0.000213 0.000060 0.000022 0.000503 0.000133 0.000059 0.000021
Nucleus 0.009770 0.003495 0.037053 0.008087 0.007281 0.005065 0.010384 0.008128 0.008941 0.004271 0.013465 0.008119
Peroxisomes 0.009721 0.006618 0.001275 0.001527 0.009632 0.008807 0.001345 0.001300 0.009691 0.007699 0.001337 0.001349
Vacuole 0.020830 0.011642 0.101117 0.048902 0.009291 0.014225 0.026446 0.030500 0.016985 0.012918 0.035072 0.034443
Vacuole Periphery 0.001338 0.000959 0.000201 0.000379 0.001152 0.000895 0.000483 0.000418 0.001276 0.000928 0.000450 0.000410

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.28 -9.54 -10.20 -9.16 -4.75 -5.44 -12.19 -20.62 -18.20 -14.72 -7.79 -14.29 -22.65 -20.16 -15.71
Bud Neck 3.13 6.47 3.68 1.49 -1.85 -8.43 -12.56 -7.57 0.63 4.45 -0.59 -3.86 0.50 1.10 4.23
Bud Site -6.26 -12.35 -15.00 -12.17 -2.68 -8.77 -13.45 -25.97* -18.40 -16.43 -9.86 -15.74 -29.52 -22.59 -17.07
Cell Periphery -17.86* -6.61 -16.80 -7.04 -9.68 13.19 -4.57 3.60 -13.02 11.69 -4.04 -26.36 -15.55 -13.22 8.43
Cytoplasm 2.96 26.23 35.05 32.21 4.32 -4.45 16.73 18.40 30.24 3.50 -0.22 33.84 37.79 41.15 5.99
Cytoplasmic Foci 15.79 8.37 14.02 1.40 5.70 -6.33 6.63 4.97 11.34 -2.89 10.68 18.47 17.99 10.80 0.28
Eisosomes 3.49 2.47 -3.72 -5.60 -5.00 -1.87 -1.18 -3.07 -1.28 -2.58 1.55 1.38 -3.21 -4.76 -4.68
Endoplasmic Reticulum 2.87 -12.34 -4.52 -5.88 10.88 3.80 -1.57 3.29 -1.07 7.21 4.70 -10.38 0.55 -4.31 10.87
Endosome 10.26 8.54 8.98 1.03 2.81 -5.20 7.14 1.01 4.78 -2.40 7.40 12.60 9.34 3.90 -1.86
Golgi 10.83 12.87 9.45 1.83 1.06 -4.84 6.38 4.45 8.66 -2.30 6.14 16.85 14.12 8.70 -1.79
Lipid Particles 11.07 7.54 10.98 -0.03 9.73 -3.53 3.35 4.53 6.53 1.52 9.46 10.85 11.73 6.35 3.31
Mitochondria 5.34 11.76 11.77 8.63 -0.04 0.85 7.85 6.05 6.22 -1.64 3.99 13.83 10.21 7.36 -1.53
Mitotic Spindle -1.98 -10.25 -3.03 -1.24 -1.02 -2.48 -6.50 -2.19 -1.84 -1.85 -3.26 -10.97 -2.30 -1.96 -1.86
None 4.91 4.75 5.45 5.06 2.47 0.99 1.14 1.26 3.56 2.74 2.28 2.39 2.73 3.64 3.34
Nuclear Periphery 3.58 -3.28 2.15 -2.89 8.17 0.79 -4.67 4.23 1.00 16.98 2.16 -5.50 4.61 0.72 17.93
Nuclear Periphery Foci 4.04 -12.68 -7.10 -8.90 11.37 0.48 -3.08 0.03 -0.79 7.81 1.98 -12.22 -3.11 -5.92 11.11
Nucleolus 2.92 2.95 3.41 15.04 12.28 1.21 1.49 1.54 1.62 14.60 1.68 2.11 2.27 1.89 16.84
Nucleus 12.06 -10.31 1.20 -5.02 10.29 1.55 -1.91 -0.57 -5.01 3.24 7.39 -5.77 0.86 -8.40 7.71
Peroxisomes 4.33 16.06 12.71 7.74 -0.58 0.77 9.54 9.31 10.04 0.19 3.21 18.77 17.27 13.19 -0.06
Vacuole 7.96 -12.83 -6.84 -9.06 7.02 -5.20 -16.29 -13.27 -10.43 -2.81 4.99 -14.21 -11.25 -14.29 0.04
Vacuole Periphery 1.46 9.99 4.58 1.96 -0.97 1.28 2.09 3.38 3.57 0.23 2.22 3.32 6.45 3.30 0.17