ORF | |
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Description | Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 121 | 82 | 50 | 33 | 94 | 57 | – | 49 | 32 | 70 | 42 | 55 | 44 | 77 | 52 | 32 | 35 | 37 | 31 | 28 | 11 | 11 | 22 | 19 |
Bud | 33 | 12 | 23 | 16 | 21 | 22 | – | 51 | 26 | 27 | 24 | 26 | 9 | 11 | 6 | 4 | 5 | 6 | 8 | 4 | 3 | 0 | 3 | 4 |
Bud Neck | 10 | 11 | 5 | 0 | 3 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 60 | 44 | 22 | 11 | 26 | 27 |
Bud Site | 10 | 7 | 4 | 3 | 6 | 2 | – | 5 | 1 | 2 | 2 | 1 | 0 | 1 | 1 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 203 | 169 | 102 | 161 | 158 | 164 | – | 176 | 151 | 174 | 180 | 181 | 145 | 212 | 262 | 74 | 89 | 98 | 381 | 287 | 194 | 88 | 195 | 184 |
Cytoplasm | 162 | 180 | 116 | 80 | 121 | 165 | – | 296 | 173 | 174 | 141 | 142 | 111 | 161 | 178 | 19 | 24 | 24 | 29 | 25 | 15 | 6 | 17 | 15 |
Endoplasmic Reticulum | 2 | 10 | 3 | 1 | 11 | 14 | – | 5 | 8 | 8 | 6 | 6 | 1 | 1 | 5 | 0 | 5 | 6 | 9 | 12 | 9 | 4 | 13 | 10 |
Endosome | 1 | 0 | 0 | 2 | 4 | 2 | – | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 3 | 1 | 1 | 2 | 2 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 3 | 0 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 29 | 18 | 65 | 176 | 86 | 176 | – | 308 | 213 | 247 | 239 | 272 | 19 | 25 | 30 | 14 | 14 | 14 | 14 | 11 | 9 | 4 | 6 | 6 |
Nucleus | 3 | 1 | 1 | 0 | 1 | 1 | – | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | – | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2 | 1 | 2 | 13 | 23 | 18 | – | 22 | 8 | 13 | 8 | 11 | 7 | 13 | 20 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 10 | 13 | 5 | 3 | 7 | 3 | – | 11 | 6 | 3 | 3 | 5 | 3 | 6 | 6 | 7 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 1 | 2 | 5 | – | 21 | 14 | 1 | 4 | 6 | 0 | 1 | 3 | 1 | 0 | 4 | 19 | 13 | 1 | 8 | 3 | 5 |
Unique Cell Count | 407 | 356 | 240 | 287 | 356 | 402 | 663 | 414 | 482 | 403 | 446 | 223 | 337 | 375 | 120 | 141 | 143 | 562 | 440 | 276 | 143 | 297 | 283 | |
Labelled Cell Count | 586 | 505 | 378 | 490 | 539 | 629 | 953 | 633 | 722 | 652 | 705 | 339 | 508 | 565 | 159 | 186 | 203 | 562 | 440 | 276 | 143 | 297 | 283 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | 6.1 | 5.0 | 5.6 | 6.0 | 5.2 | – | 5.2 | 4.9 | 5.1 | 5.1 | 5.1 | 7.1 | 7.0 | 6.9 | 11.1 | 10.8 | 10.3 | 6.3 | 7.3 | 7.1 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.6 | 1.7 | 1.3 | 1.9 | – | 1.3 | 1.1 | 0.9 | 1.3 | 1.2 | 1.5 | 1.3 | 1.4 | 2.0 | 2.1 | 1.9 | 1.5 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.17 | 0.26 | 0.08 | – | 0.08 | -0.03 | 0.04 | 0.03 | 0.03 | 0.51 | 0.5 | 0.47 | 1.16 | 1.12 | 1.06 | 0.35 | 0.56 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.9 | 1.6 | -2.2 | – | -5.7 | 0 | -2.1 | -3.6 | -2.9 | -0.3 | 0.6 | -2.3 | 1.2 | 0.9 | 1.1 |
Bud | -1.8 | -1.7 | -2.0 | – | -0.9 | 0 | -2.0 | -1.7 | -1.8 | -2.4 | -3.2 | -4.6 | -2.1 | -2.2 | -1.9 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.1 | 0.5 | -0.4 | – | -4.6 | 0 | -1.7 | 0.5 | -0.5 | 4.9 | 4.9 | 6.7 | 3.4 | 3.9 | 4.9 |
Cytoplasm | -4.8 | -3.5 | -1.8 | – | -1.0 | -1.6 | -3.2 | -3.3 | -4.3 | 0.3 | -0.1 | -0.2 | -6.0 | -6.1 | -6.2 |
Endoplasmic Reticulum | 0 | 1.5 | 1.7 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7.9 | -0.8 | 4.2 | – | 5.2 | 0 | 6.2 | 7.9 | 8.5 | -5.2 | -6.4 | -6.4 | -3.3 | -4.0 | -4.0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 2.5 | 3.4 | 2.6 | – | 2.1 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.9 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 2.4 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9638 | 3.9584 | 3.6156 | 3.3888 | 2.995 | 3.7121 | 3.3494 | 3.6402 | 3.2561 | 2.8984 | 3.1781 | 3.3064 | 5.6603 | 5.1634 | 4.7356 | 4.6553 | 4.5173 | 5.0617 |
Actin | 0.0103 | 0.0015 | 0.0073 | 0.0514 | 0.0417 | 0.018 | 0.0626 | 0.0028 | 0.0162 | 0.0182 | 0.0066 | 0.0009 | 0.0368 | 0.0009 | 0.0131 | 0.0091 | 0.0041 | 0.0018 |
Bud | 0.0003 | 0.0005 | 0.0008 | 0.0009 | 0.0006 | 0.0005 | 0.0072 | 0.0009 | 0.0016 | 0.0008 | 0.0037 | 0.0017 | 0.0007 | 0.0007 | 0.0011 | 0.0009 | 0.0002 | 0.0005 |
Bud Neck | 0.0017 | 0.0055 | 0.0146 | 0.0101 | 0.01 | 0.062 | 0.0081 | 0.0082 | 0.0058 | 0.0092 | 0.0038 | 0.0489 | 0.0034 | 0.0066 | 0.006 | 0.0009 | 0.0004 | 0.0501 |
Bud Periphery | 0.0024 | 0.0028 | 0.0125 | 0.012 | 0.0084 | 0.0083 | 0.0152 | 0.0048 | 0.0141 | 0.0083 | 0.0139 | 0.0098 | 0.0046 | 0.005 | 0.015 | 0.0276 | 0.0027 | 0.0092 |
Bud Site | 0.0116 | 0.1437 | 0.0387 | 0.0075 | 0.0038 | 0.0026 | 0.0273 | 0.1678 | 0.04 | 0.0054 | 0.0193 | 0.0016 | 0.0112 | 0.1795 | 0.0361 | 0.0008 | 0.0005 | 0.002 |
Cell Periphery | 0.8156 | 0.7588 | 0.7832 | 0.7569 | 0.8705 | 0.8318 | 0.7043 | 0.6994 | 0.7977 | 0.8265 | 0.745 | 0.8721 | 0.8048 | 0.6487 | 0.7877 | 0.848 | 0.7735 | 0.8616 |
Cytoplasm | 0.0694 | 0.0808 | 0.0933 | 0.0779 | 0.045 | 0.0549 | 0.0531 | 0.0983 | 0.082 | 0.0603 | 0.0476 | 0.0517 | 0.0586 | 0.1404 | 0.0997 | 0.0599 | 0.0719 | 0.0533 |
Cytoplasmic Foci | 0.0125 | 0.0001 | 0.0054 | 0.0096 | 0.0018 | 0.0011 | 0.0107 | 0.0007 | 0.0019 | 0.0011 | 0.0245 | 0.0002 | 0.0104 | 0.0027 | 0.008 | 0.0124 | 0.0027 | 0.0003 |
Eisosomes | 0.0077 | 0.0021 | 0.0023 | 0.0015 | 0.0008 | 0.0011 | 0.0185 | 0.0024 | 0.0009 | 0.0025 | 0.0008 | 0.0006 | 0.0034 | 0.0008 | 0.0016 | 0.0003 | 0.0003 | 0.0007 |
Endoplasmic Reticulum | 0.0131 | 0.0011 | 0.0035 | 0.005 | 0.0057 | 0.0017 | 0.0139 | 0.0053 | 0.0073 | 0.0166 | 0.0168 | 0.0027 | 0.009 | 0.0013 | 0.0085 | 0.0039 | 0.0024 | 0.0023 |
Endosome | 0.0128 | 0 | 0.0068 | 0.0126 | 0.0018 | 0.0013 | 0.0117 | 0.0001 | 0.0027 | 0.0075 | 0.021 | 0.0003 | 0.0072 | 0.0004 | 0.001 | 0.0011 | 0.0122 | 0.0003 |
Golgi | 0.0049 | 0.0001 | 0.0018 | 0.0085 | 0.0037 | 0.0028 | 0.0124 | 0.0003 | 0.0032 | 0.0068 | 0.0062 | 0.0004 | 0.0051 | 0.0003 | 0.0011 | 0.0015 | 0.0024 | 0.0011 |
Lipid Particles | 0.0124 | 0.0001 | 0.0122 | 0.0133 | 0.0006 | 0.0026 | 0.0087 | 0.0042 | 0.0021 | 0.0038 | 0.0156 | 0.0003 | 0.0074 | 0.0013 | 0.0017 | 0.0025 | 0.017 | 0.0018 |
Mitochondria | 0.0021 | 0 | 0.0013 | 0.0062 | 0.0003 | 0.005 | 0.0149 | 0.0003 | 0.0046 | 0.0043 | 0.013 | 0.0045 | 0.0014 | 0 | 0.0006 | 0.0002 | 0.0432 | 0.0056 |
None | 0.0024 | 0.0002 | 0.004 | 0.0012 | 0.0006 | 0.0011 | 0.0035 | 0.0006 | 0.0072 | 0.0198 | 0.0202 | 0.0006 | 0.0169 | 0.0016 | 0.0019 | 0.0006 | 0.0044 | 0.0029 |
Nuclear Periphery | 0.0033 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0038 | 0.0004 | 0.0025 | 0.0016 | 0.0044 | 0.0001 | 0.0036 | 0.0003 | 0.0006 | 0.0004 | 0.0213 | 0.0001 |
Nucleolus | 0.0004 | 0 | 0.0002 | 0.0006 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0033 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 |
Nucleus | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.002 | 0.0003 | 0.0006 | 0.0009 | 0.0046 | 0.0002 | 0.0009 | 0.0002 | 0.0004 | 0.0001 | 0.0138 | 0.0002 |
Peroxisomes | 0.0101 | 0 | 0.007 | 0.0182 | 0.0002 | 0.0015 | 0.0056 | 0 | 0.0028 | 0.0001 | 0.0049 | 0 | 0.0052 | 0.0012 | 0.0008 | 0.0158 | 0.0069 | 0.0016 |
Punctate Nuclear | 0.0015 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0002 | 0.0003 | 0.0133 | 0 | 0.0043 | 0.0055 | 0.011 | 0.0089 | 0.0138 | 0 |
Vacuole | 0.0048 | 0.0023 | 0.0042 | 0.0048 | 0.0039 | 0.003 | 0.0133 | 0.003 | 0.0058 | 0.0049 | 0.0095 | 0.0033 | 0.0045 | 0.0026 | 0.0038 | 0.0049 | 0.0042 | 0.0041 |
Vacuole Periphery | 0.0005 | 0 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0013 | 0.0001 | 0.0003 | 0.0008 | 0.0019 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0011 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.6501 | 51.7543 | 47.4401 | 61.804 | 61.8166 | 44.2479 | 48.481 | 66.4816 | 76.997 | 54.1361 |
Translational Efficiency | 2.4934 | 2.5984 | 2.4031 | 1.7494 | 1.7149 | 2.0967 | 2.678 | 1.7545 | 1.3762 | 2.1904 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |