Standard name
Human Ortholog
Description Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis; subject to proteolytic cleavage by separate Esp1p, resulting in dissociation of cohesin from chromatin and the separation of sister chromatids at the mitotic metaphase-to-anaphase transition; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.15 0.11 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.37 0.39 0.4 0.4 0.3 0.39 0.41 0.44 0.35 0.36 0.35 0.49 0.44 0.45 0.22 0.23 0.26 0.19 0.16 0.18 0.66 0.69 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.19 0.16 0 0 0 0 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0.05 0 0.09 0.15 0.25 0.22 0.42 0.33 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.37 0.34 0.42 0.33 0.38 0.31 0.26 0.17 0.23 0.19 0.21 0.41 0.31 0.29 0.31 0.27 0.27 0.31 0.38 0.35 0.15 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.16 0.15 0.1 0.18 0.27 0.18 0.19 0.21 0.24 0.2 0.22 0.08 0.25 0.23 0.14 0.15 0.18 0.13 0.1 0.11 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.11 0.1 0.11 0 0 0
Vac/Vac Membrane 0.08 0.28 0.11 0.11 0.13 0.11 0.08 0 0.05 0.05 0 0.08 0.07 0 0.24 0.23 0.25 0.06 0.07 0.1 0.09 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2 5 6 2 5 5 0 0 0 0 0 0 33 23 25 1 3 1
Bud Neck 0 0 2 0 0 0 3 7 7 4 6 0 0 0 0 0 0 1 1 0 0 1 0
Bud Site 1 1 0 0 0 0 3 3 4 6 8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 97 48 80 91 77 126 173 127 120 126 153 71 54 83 50 50 56 56 23 42 23 68 108
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 0 12 12 7 1 0 0 0 0 0
Endosome 9 3 5 5 5 13 9 1 5 3 4 3 5 6 47 41 34 5 1 1 0 4 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 4 0 1 0 1 0
Mitochondria 20 1 0 11 4 29 63 72 74 146 144 0 0 0 9 7 5 6 0 2 0 1 2
Nucleus 95 42 85 75 97 102 110 48 78 65 93 60 38 53 70 57 58 90 58 80 5 4 0
Nuclear Periphery 0 0 0 0 0 2 1 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 41 18 21 41 68 60 78 62 80 71 98 12 31 43 32 33 38 37 15 26 0 0 0
Peroxisomes 2 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0 0
SpindlePole 5 1 4 8 1 0 7 2 13 3 12 2 1 9 4 11 10 33 15 25 0 3 3
Vac/Vac Membrane 21 35 22 24 33 35 34 6 17 19 17 11 9 2 55 50 54 16 11 22 3 5 4
Unique Cell Count 259 124 202 225 254 327 419 290 340 350 437 146 122 183 228 215 213 291 153 234 35 100 125
Labelled Cell Count 291 149 219 255 285 369 487 335 403 449 540 159 140 197 280 265 263 291 153 234 35 100 125


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.9 6.9 6.4 7.4 6.0 6.1 4.9 5.4 4.4 4.9 7.6 8.2 8.1 9.4 9.5 9.4 5.4 5.5
Std Deviation (1e-4) 1.9 2.8 2.1 1.9 2.1 1.8 2.4 1.9 2.1 1.6 1.8 2.8 3.0 3.0 3.9 4.0 3.6 1.7 1.2
Intensity Change (Log2) -0.11 0.09 -0.21 -0.19 -0.51 -0.36 -0.67 -0.51 0.13 0.24 0.23 0.44 0.46 0.43 -0.37 -0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -2.1 -0.2 0.4 0.9 -1.0 -0.8 -1.1 1.7 0.8 1.1 -4.0 -3.6 -2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.3 3.4 0
Endosome 0 0 0.9 0 0 0 0 0 0 0 0.5 5.8 5.4 4.7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 0 4.4 5.8 0 7.1 0 0 0 0 0 0 0 0
Nucleus -1.9 -0.8 -2.5 -4.0 -6.3 -4.7 -6.0 -5.5 -0.2 -2.0 -2.7 -2.5 -3.4 -3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.3 4.4 2.5 2.6 0 3.8 0 0 -0.7 3.6 3.4 1.1 1.5 2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.0 0 0 0 0 1.2 0 0 0 0 1.6 0 1.7 1.5
Vacuole -0.1 0.7 -0.1 -1.1 -4.2 -2.6 -2.4 -3.4 -1.1 -1.0 -4.0 3.6 3.3 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2134 3.1189 3.5077 3.1182 1.3707 0.7584 0.8542 4.3337 4.702 4.2125 2.5589 1.8665 -0.9046 2.4349 2.6701 1.6178 1.0084 0.2998
Actin 0.0344 0.0037 0.0112 0.0001 0.0119 0.0061 0.0293 0.0002 0.0095 0.0003 0.004 0.0013 0.0295 0.0001 0.0181 0.0004 0.0042 0.0022
Bud 0.005 0.0067 0.0021 0.0001 0.0009 0.0023 0.0065 0.0016 0.0008 0.0001 0.0002 0.0006 0.0103 0.0061 0.0006 0.0002 0.0002 0.0004
Bud Neck 0.0061 0.0049 0.0011 0.0003 0.0017 0.0056 0.007 0.0007 0.001 0.0006 0.0012 0.0057 0.0073 0.0005 0.0013 0.0002 0.0006 0.0055
Bud Periphery 0.0054 0.0051 0.0014 0.0001 0.001 0.0029 0.0073 0.0011 0.0011 0.0004 0.0004 0.0009 0.0114 0.0027 0.0006 0.0005 0.0003 0.0006
Bud Site 0.0104 0.0202 0.0015 0.0001 0.009 0.0005 0.0134 0.0022 0.0015 0.0005 0.0004 0.0004 0.0138 0.0058 0.0053 0.001 0.0011 0.0005
Cell Periphery 0.001 0.0003 0.0001 0 0.0002 0.0002 0.0012 0.0001 0.0002 0.0005 0.0001 0.0001 0.001 0.0002 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.0488 0.0151 0.0008 0.0001 0.0209 0.0408 0.0278 0.0254 0.001 0.009 0.0293 0.0416 0.0641 0.0267 0.0007 0.0005 0.0516 0.0431
Cytoplasmic Foci 0.014 0.0031 0.0008 0 0.0075 0.0158 0.0207 0.0011 0.0032 0.0032 0.0037 0.0026 0.0179 0.0012 0.0066 0.0077 0.0171 0.0016
Eisosomes 0.0004 0 0.0001 0 0.0001 0 0.0005 0 0.0001 0 0 0 0.0003 0 0.0001 0 0 0
Endoplasmic Reticulum 0.012 0.0009 0.0001 0 0.0037 0.001 0.0024 0.0007 0.0014 0.0023 0.001 0.0016 0.0043 0.002 0.0005 0.0009 0.0121 0.0014
Endosome 0.0248 0.0013 0.001 0 0.0069 0.0023 0.0104 0.001 0.0026 0.011 0.0269 0.0024 0.0194 0.0004 0.0062 0.0056 0.0115 0.0046
Golgi 0.008 0.0003 0.0004 0 0.0023 0.0019 0.0042 0 0.0014 0.0003 0.009 0.0004 0.0067 0 0.0049 0.0014 0.0037 0.0006
Lipid Particles 0.0101 0.0003 0.0006 0 0.0023 0.004 0.0049 0.0001 0.0066 0.0017 0.0055 0.0017 0.0102 0.0001 0.0106 0.0271 0.0144 0.0014
Mitochondria 0.0078 0.0009 0.0086 0.0004 0.001 0.0004 0.0028 0.0004 0.0123 0.0004 0.0036 0.0009 0.0058 0.0003 0.0095 0.0086 0.0018 0.0005
None 0.0228 0.0168 0.0012 0.0001 0.0523 0.0148 0.0759 0.029 0.0011 0.0051 0.0073 0.0259 0.0602 0.031 0.0003 0.0006 0.006 0.027
Nuclear Periphery 0.0481 0.0095 0.0049 0.0028 0.0198 0.0092 0.0087 0.0104 0.0101 0.0123 0.0068 0.011 0.02 0.0071 0.0049 0.0072 0.0201 0.0208
Nucleolus 0.1104 0.0586 0.079 0.0605 0.0183 0.0907 0.1149 0.0429 0.088 0.0888 0.0509 0.0605 0.085 0.0258 0.0688 0.0444 0.0374 0.0426
Nucleus 0.5079 0.7884 0.8392 0.8767 0.7098 0.6521 0.5669 0.8447 0.8323 0.8319 0.7707 0.7308 0.5392 0.8598 0.837 0.8777 0.6516 0.7087
Peroxisomes 0.004 0.0001 0.0003 0 0.0012 0.0076 0.0035 0.0001 0.0004 0.0001 0.0015 0.0014 0.0101 0.0001 0.005 0.0019 0.0682 0.0002
Punctate Nuclear 0.1033 0.0607 0.0451 0.0587 0.1264 0.1394 0.0817 0.0368 0.0237 0.0283 0.0656 0.1086 0.0718 0.0287 0.0175 0.0063 0.0949 0.1367
Vacuole 0.0108 0.0026 0.0005 0.0001 0.0022 0.002 0.0081 0.0012 0.0009 0.0027 0.0071 0.0013 0.0094 0.0012 0.0009 0.0041 0.0025 0.001
Vacuole Periphery 0.0044 0.0005 0.0002 0.0001 0.0007 0.0003 0.0019 0.0002 0.0007 0.0004 0.0049 0.0004 0.0024 0.0002 0.0005 0.0036 0.0007 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.0023 7.5384 4.7333 19.1152 44.9957 51.8657 13.6319 8.5081 11.9808 50.6615
Translational Efficiency 2.2815 2.0924 1.3791 1.4688 1.7298 2.2909 1.2535 1.1499 1.3207 1.491

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
86 1466 1880 683 1367 2241 401 1579 1453 3707 2281 2262

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 697.61 840.71 1109.88 1154.26 767.85 785.17 1103.43 1079.39 763.69 807.13 1108.75 1102.00
Standard Deviation 147.54 187.75 212.75 221.32 133.27 147.12 170.35 211.17 135.18 166.62 205.94 217.02
Intensity Change Log 2 0.269188 0.669911 0.726476 0.032181 0.523099 0.491320 0.149867 0.594852 0.608050

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000795 0.001335 0.000565 0.002230 0.000731 0.000760 0.001513 0.001162 0.000735 0.000987 0.000732 0.001485
Bud Neck 0.004177 0.023704 0.036190 0.036204 0.011733 0.031154 0.014665 0.030627 0.011286 0.028208 0.032406 0.032311
Bud Site 0.002365 0.010945 0.006062 0.021010 0.006707 0.010963 0.009291 0.010760 0.006450 0.010956 0.006630 0.013855
Cell Periphery 0.000582 0.000853 0.000248 0.000288 0.000732 0.000654 0.000180 0.000266 0.000723 0.000733 0.000236 0.000273
Cytoplasm 0.065911 0.102288* 0.051375 0.016144 0.162507* 0.100632* 0.036281 0.032811 0.156790* 0.101287* 0.048721 0.027779
Cytoplasmic Foci 0.011856 0.025380 0.000567 0.002749 0.029829 0.012336 0.002294 0.002343 0.028765 0.017495 0.000871 0.002465
Eisosomes 0.000065 0.000079 0.000023 0.000044 0.000066 0.000051 0.000069 0.000037 0.000066 0.000062 0.000031 0.000039
Endoplasmic Reticulum 0.002702 0.003938 0.001612 0.003388 0.003021 0.002967 0.005053 0.002606 0.003003 0.003351 0.002217 0.002842
Endosome 0.002082 0.004626 0.000521 0.004913 0.003428 0.004114 0.001155 0.003993 0.003348 0.004317 0.000633 0.004270
Golgi 0.000866 0.001457 0.000268 0.006386 0.001363 0.001735 0.000251 0.003058 0.001333 0.001625 0.000265 0.004063
Lipid Particles 0.002824 0.003853 0.000135 0.000325 0.003629 0.001554 0.001023 0.000273 0.003581 0.002463 0.000291 0.000288
Mitochondria 0.001744 0.008657 0.001836 0.005733 0.001943 0.006303 0.001643 0.005709 0.001931 0.007234 0.001802 0.005716
Mitotic Spindle 0.075714 0.018996 0.014476 0.126839* 0.012804 0.027654 0.011255 0.057532 0.016527 0.024230 0.013910 0.078459
None 0.025678 0.025142 0.008149 0.005184 0.031827 0.022496 0.006049 0.011286 0.031463 0.023543 0.007780 0.009444
Nuclear Periphery 0.001432 0.001135 0.001246 0.001826 0.000902 0.001354 0.000975 0.001592 0.000933 0.001268 0.001199 0.001663
Nuclear Periphery Foci 0.008253 0.004896 0.000398 0.000741 0.006626 0.003426 0.000794 0.000639 0.006723 0.004007 0.000468 0.000670
Nucleolus 0.299642* 0.137002 0.047562 0.096786 0.144925 0.107675 0.043121 0.048772 0.154083 0.119273 0.046781 0.063270
Nucleus 0.487383* 0.612297* 0.823518* 0.653887* 0.568466* 0.655864* 0.839659* 0.773867* 0.563667* 0.638635* 0.826356* 0.737640*
Peroxisomes 0.004256 0.004210 0.000685 0.004806 0.002986 0.002632 0.002979 0.001549 0.003062 0.003256 0.001088 0.002533
Vacuole 0.001172 0.007968 0.004214 0.007705 0.005061 0.004254 0.021498 0.007735 0.004831 0.005723 0.007253 0.007726
Vacuole Periphery 0.000501 0.001239 0.000348 0.002810 0.000712 0.001420 0.000253 0.003381 0.000700 0.001349 0.000331 0.003208

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.43 0.03 -5.67 -3.45 -6.65 -0.69 -7.14 -10.45 -10.22 3.49 -4.72 -5.38 -10.98 -6.92 -7.43
Bud Neck -13.16 -24.90 -13.09 -5.91 2.09 -12.73 -5.56 -15.04 -3.93 -5.94 -14.14 -21.02 -17.87 -6.99 2.13
Bud Site -8.18 -7.78 -5.48 -1.06 -2.51 -4.03 -1.01 -1.82 1.42 0.10 -5.21 -2.29 -3.46 0.45 -1.76
Cell Periphery -1.06 1.39 1.34 9.15 -0.39 1.27 9.19 8.94 9.78 -0.57 -0.04 8.74 8.88 13.51 0.76
Cytoplasm -2.25 1.07 2.44 14.07 6.76 7.60 16.43 18.03 14.54 -0.01 7.44 16.81 18.87 19.65 5.10
Cytoplasmic Foci -2.78 2.33 2.22 11.17 -1.33 6.75 12.25 12.62 9.50 1.04 4.55 13.01 12.82 14.58 -0.90
Eisosomes -1.32 5.68 2.38 4.48 -8.32 5.97 -2.57 9.87 4.82 7.47 1.65 10.25 9.25 6.64 -1.77
Endoplasmic Reticulum -3.04 1.89 -3.34 -1.19 -6.11 0.37 -6.83 -1.41 -1.78 6.14 -1.81 0.94 -3.09 -1.66 -4.22
Endosome -3.61 3.06 -0.19 2.84 -2.50 -1.46 5.95 2.91 3.81 -0.96 -2.30 9.42 2.97 4.84 -3.43
Golgi -1.99 3.73 -1.05 -0.66 -1.64 -0.51 3.22 0.05 0.44 -1.93 -0.58 3.43 -0.47 -0.11 -2.55
Lipid Particles -1.03 2.12 1.76 5.59 -3.62 4.00 4.87 7.24 7.56 4.13 1.87 7.33 7.26 8.30 -0.43
Mitochondria -3.94 -1.94 -5.38 1.64 -4.97 -4.33 -0.26 -4.41 1.41 -4.84 -5.58 -0.71 -5.71 2.23 -6.54
Mitotic Spindle 2.84 2.94 -1.54 -9.06 -9.35 -4.33 -0.96 -9.50 -6.52 -6.55 -2.49 -0.14 -11.96 -10.92 -12.32
None 0.06 2.48 2.74 12.35 3.80 4.00 12.09 9.89 8.89 -4.64 3.70 11.92 10.96 13.74 -2.38
Nuclear Periphery 0.87 -0.26 -4.58 -7.34 -6.48 -3.24 -3.79 -6.55 -3.36 -3.53 -3.01 -4.82 -8.95 -6.73 -6.55
Nuclear Periphery Foci 1.34 3.50 3.35 6.49 -2.40 3.15 5.51 6.17 4.95 3.73 2.85 6.52 6.50 7.96 -0.30
Nucleolus 6.28 8.29 7.44 4.08 -2.32 5.80 8.32 13.33 9.62 1.04 6.03 11.80 13.19 10.73 1.56
Nucleus -4.22 -9.69 -5.20 -2.59 9.16 -8.88 -15.11 -17.79 -10.55 2.37 -8.54 -23.81 -16.36 -10.70 6.75
Peroxisomes -0.08 3.80 1.67 2.64 -3.67 0.91 -0.85 5.52 3.86 4.30 -0.59 4.35 4.28 5.26 0.17
Vacuole -7.05 -5.73 -6.67 -5.16 -5.68 1.43 -4.72 -4.79 -5.37 3.04 -1.30 -4.14 -7.55 -7.13 -4.32
Vacuole Periphery -5.26 1.11 -1.77 -0.17 -1.99 -2.35 6.23 -1.27 -0.23 -1.81 -3.39 5.59 -1.61 -0.31 -2.23