Standard name
Description Non-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.18 0.43 0.62 0.72 0.62 0.61 0.79 0.86 0.88 0.85 0.9 0.78 0.91 0.96 0.16 0.16 0.17 0 0.05 0.05 0.07 0.07 0.08
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.09 0 0 0 0.14 0 0.05 0.05 0.12 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.15 0.09 0.07 0.09 0.08 0.1 0 0 0 0 0 0 0 0 0.41 0.3 0.39 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.71 0.62 0.5 0.45 0.49 0.38 0.38 0.19 0.16 0.16 0.12 0.28 0.13 0.07 0.56 0.7 0.54 0.85 0.84 0.89 0.83 0.81 0.75
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 0 1 2
Bud 0 0 0 0 0 0 0 4 5 0 1 0 0 0 0 0 1 4 3 2 1 5 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 79 161 108 144 147 134 243 330 374 152 240 131 308 374 68 53 61 5 20 9 16 28 27
Endoplasmic Reticulum 1 5 1 0 0 1 0 0 0 0 0 2 2 0 29 12 19 0 1 0 1 1 3
Endosome 39 3 4 4 2 0 3 1 2 1 1 7 10 1 8 10 7 15 10 2 4 18 19
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 8 2 3 2 0 1 0 1 0
Mitochondria 63 33 1 4 3 30 2 20 22 21 14 0 2 0 12 2 18 9 10 1 5 10 11
Nucleus 64 34 13 18 19 23 8 5 4 0 3 1 0 1 168 100 140 20 2 0 1 1 2
Nuclear Periphery 1 1 0 0 2 2 4 1 2 3 4 0 0 1 1 0 3 2 0 0 1 2 3
Nucleolus 10 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 4 0 0 0 1 0
Peroxisomes 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 0 0 1 0 2 0 0 0 0 0 0 0 1 3 4 1 1 2 2
Vac/Vac Membrane 312 233 87 90 117 84 117 74 68 29 33 47 43 26 231 230 193 394 347 175 190 339 240
Unique Cell Count 441 377 175 199 239 220 307 385 424 178 266 167 337 391 413 330 357 466 412 197 229 418 322
Labelled Cell Count 572 471 214 261 290 275 378 435 480 206 296 188 366 403 525 409 448 466 412 197 229 418 322


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.9 5.6 5.7 6.1 4.3 5.4 4.6 4.7 4.5 4.6 5.3 5.3 5.1 7.8 8.2 7.0 4.9 5.1 5.2
Std Deviation (1e-4) 1.5 1.3 1.9 2.1 1.4 1.2 1.6 1.2 1.0 1.4 1.0 1.1 1.7 1.2 2.3 2.3 2.0 1.7 1.7 1.7
Intensity Change (Log2) 0.04 0.12 -0.38 -0.05 -0.3 -0.26 -0.3 -0.28 -0.09 -0.08 -0.14 0.48 0.55 0.31 -0.2 -0.13 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 1.4 4.4 0.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nhp10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available