Standard name
Description Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.11 0.15 0.19 0.2 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.08 0.12 0.05 0.16 0.16 0.16 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.89 0.95 0.97 0.95 0.9 0.85 0.9 0.82 0.83 0.82 0.82 0.94 0.87 0.81 0.53 0.45 0.44 0.82 0.86 0.89 0.81 0.79 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.11 0.12 0.17 0.18 0.23 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.16 0 0 0 0 0 0 0 0 0 0 0 0 0 0.35 0.39 0.35 0.05 0.08 0 0.07 0.08 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1
Bud 0 0 0 0 0 0 3 1 2 2 1 2 0 0 0 0 0 0 7 1 3 4 11 17
Bud Neck 0 0 0 0 0 0 0 2 2 0 1 2 0 0 0 0 0 0 0 0 0 0 2 2
Bud Site 0 0 0 0 1 3 6 12 10 15 20 23 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 2 6 7 12 10 25 10 7 9 8 13 20 56 78 25 36 26 7 2 2 9 12 11
Endoplasmic Reticulum 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 7 11 8 0 0 1 0 0 2
Endosome 7 0 1 0 0 1 1 1 0 1 0 0 0 2 2 3 2 5 4 0 1 0 9 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 0 0 0 2 0
Mitochondria 24 4 1 4 2 29 66 26 66 80 97 82 0 0 0 3 7 3 6 0 2 0 7 7
Nucleus 265 103 271 235 336 326 466 456 343 417 504 701 339 444 409 68 81 47 283 108 264 176 441 358
Nuclear Periphery 5 0 2 2 2 1 10 11 3 4 3 14 0 0 0 0 0 1 0 0 0 0 0 2
Nucleolus 4 0 3 2 4 20 61 62 70 91 138 193 0 4 4 1 0 1 4 1 2 0 10 15
Peroxisomes 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 1 1 2 1 2 6 0 0 0 5 0 4 4 11 12
Vac/Vac Membrane 26 18 5 0 2 1 1 1 1 1 2 2 5 2 8 45 70 37 17 9 10 14 43 72
Unique Cell Count 316 116 286 242 355 363 547 504 419 504 613 858 362 508 504 129 179 106 344 126 298 217 557 513
Labelled Cell Count 341 127 290 250 360 391 641 582 504 621 775 1034 365 512 509 153 209 128 344 126 298 217 557 513


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.6 6.6 6.2 6.7 5.9 5.8 5.9 5.6 5.4 5.3 5.6 7.9 7.9 7.5 8.9 8.6 9.9 7.2 7.3 7.9
Std Deviation (1e-4) 0.8 1.2 1.5 1.5 1.7 1.3 1.5 1.5 1.2 1.2 1.2 1.2 1.3 1.7 1.7 1.9 2.1 16.2 1.0 1.5 1.6
Intensity Change (Log2) -0.11 0.02 -0.18 -0.19 -0.17 -0.26 -0.3 -0.32 -0.24 0.24 0.26 0.18 0.42 0.37 0.57 0.11 0.15 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2.9 3.1 2.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 1.0 0.5 1.8 -0.1 -0.4 -0.3 0 0 2.2 4.5 5.9 6.2 6.6 7.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.6 5.9 3.6 6.8 6.9 6.9 5.2 0 0 0 0 0 0
Nucleus 1.3 -0.1 -2.3 -4.1 -2.1 -5.0 -4.8 -5.1 -5.3 -0.6 -3.3 -5.3 -10.2 -12.1 -11.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.1 5.2 5.5 6.7 7.1 8.2 8.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 9.6 10.7 9.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Maf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available