Standard name
Human Ortholog
Description Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 1.0 0.96 0.93 0.89 0.77 0.78 0.62 0.69 0.56 0.59 1.0 0.99 0.99 0.97 0.96 0.98 0.88 0.91 0.86 0.89 0.86 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.16 0.15 0.34 0.46 0.42 0.6 0.54 0.69 0.68 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 4 4
Bud 1 3 2 2 1 3 1 3 5 1 3 3 0 0 0 0 0 1 1 2 0 5 9 18
Bud Neck 0 1 0 0 1 0 1 1 2 3 0 1 0 0 0 1 1 1 1 1 0 0 1 5
Bud Site 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 2 0 0 0 0 0 1 0 1 3 1 0 2 1 5 1 0 0 0 1 1 4
Cytoplasm 286 296 214 99 152 140 150 167 90 120 68 92 771 763 866 442 372 282 255 290 186 393 416 426
Endoplasmic Reticulum 2 4 1 0 0 0 1 0 0 0 2 0 0 1 3 22 33 21 2 0 1 0 1 4
Endosome 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 5 7 1 3 0 0 4 5 7
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 1 0
Mitochondria 18 4 5 16 24 54 90 90 87 93 84 105 2 1 1 8 0 1 12 10 15 11 12 13
Nucleus 1 0 0 0 1 0 1 1 1 0 0 4 2 0 3 1 4 5 0 0 0 0 1 2
Nuclear Periphery 0 1 0 2 1 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 4 1 1 1 0 6 11 15
Vac/Vac Membrane 0 0 0 2 0 0 1 0 0 1 0 0 0 1 1 4 8 2 3 4 1 4 4 14
Unique Cell Count 295 304 215 103 163 157 194 213 145 173 121 155 773 767 871 455 387 288 291 320 216 444 482 535
Labelled Cell Count 310 313 224 122 182 197 246 264 186 218 158 210 777 767 876 487 435 316 291 320 216 444 482 535


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.3 3.6 3.3 3.6 3.1 2.8 3.2 3.0 2.7 2.9 3.0 4.7 4.7 4.9 5.0 6.1 5.4 3.9 4.1 4.7
Std Deviation (1e-4) 0.6 0.7 0.5 1.1 1.8 1.3 1.0 1.0 0.8 1.1 0.6 1.1 1.1 1.4 1.4 1.1 1.6 1.0 1.3 1.3 1.3
Intensity Change (Log2) – – – -0.1 0.01 -0.19 -0.36 -0.17 -0.25 -0.39 -0.29 -0.27 0.38 0.41 0.45 0.48 0.77 0.6 0.14 0.2 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.3073 0.0012 0.0034 0.0002 0.0007 0.0004 – – – – – – 0.0663 0.0008 0.0023 0.0003 0.0028 0.009
Bud 0.0031 0.0003 0.0005 0 0.0002 0 – – – – – – 0.0037 0.0013 0.0018 0.0047 0.0088 0.0019
Bud Neck 0.0033 0 0.0005 0 0.0002 0.0008 – – – – – – 0.0046 0.0003 0.0002 0.0039 0.0012 0.0025
Bud Periphery 0.0079 0.0004 0.0012 0 0.0002 0 – – – – – – 0.0069 0.0005 0.0039 0.004 0.0201 0.0054
Bud Site 0.0275 0.0005 0.0031 0 0.0002 0 – – – – – – 0.0155 0.0054 0.0023 0.0168 0.0014 0.0006
Cell Periphery 0.0021 0.0001 0.0007 0 0.0001 0.0001 – – – – – – 0.0005 0.0003 0.0002 0.0003 0.0002 0.0002
Cytoplasm 0.019 0.1248 0.227 0.1666 0.1401 0.3364 – – – – – – 0.0886 0.2638 0.2097 0.0929 0.1372 0.2082
Cytoplasmic Foci 0.0236 0.0252 0.0141 0.0016 0.0374 0.0063 – – – – – – 0.0372 0.007 0.006 0.0323 0.0078 0.0071
Eisosomes 0.0082 0.0002 0.0004 0.0002 0.0001 0.0001 – – – – – – 0.0009 0.0002 0.0002 0.0001 0.0003 0.0002
Endoplasmic Reticulum 0.0028 0.0061 0.0044 0.0004 0.0034 0.0014 – – – – – – 0.0032 0.0027 0.0021 0.0039 0.0024 0.0025
Endosome 0.0078 0.0038 0.009 0.0003 0.1181 0.0137 – – – – – – 0.0162 0.0024 0.0037 0.002 0.0047 0.0041
Golgi 0.0094 0.0007 0.007 0 0.011 0.0011 – – – – – – 0.0081 0.0002 0.0003 0.0003 0.0005 0.0011
Lipid Particles 0.0602 0.0021 0.0203 0.0001 0.0048 0.0002 – – – – – – 0.0207 0.0008 0.0008 0.0074 0.0016 0.0013
Mitochondria 0.0845 0.0009 0.0442 0 0.0018 0.0001 – – – – – – 0.0076 0.0004 0.001 0.0003 0.0011 0.002
None 0.3674 0.8192 0.6429 0.8294 0.6681 0.6382 – – – – – – 0.6709 0.7088 0.748 0.8177 0.7652 0.7429
Nuclear Periphery 0.0115 0.0024 0.0029 0.0001 0.002 0.0002 – – – – – – 0.0092 0.0006 0.0055 0.0011 0.0039 0.0024
Nucleolus 0.0066 0.0002 0.0005 0 0.0001 0 – – – – – – 0.0017 0.0004 0.0014 0.0016 0.0044 0.0007
Nucleus 0.0031 0.0009 0.0018 0.0002 0.0006 0.0003 – – – – – – 0.0038 0.0015 0.0034 0.0021 0.0078 0.0023
Peroxisomes 0.0319 0.0022 0.0106 0 0.0005 0.0001 – – – – – – 0.0156 0.0002 0.0009 0.0005 0.0008 0.0007
Punctate Nuclear 0.0079 0.0076 0.0037 0.0001 0.0014 0.0002 – – – – – – 0.0144 0.0016 0.0054 0.0064 0.0263 0.003
Vacuole 0.0019 0.0009 0.0016 0.0006 0.0063 0.0003 – – – – – – 0.0032 0.0008 0.0007 0.0011 0.0011 0.0013
Vacuole Periphery 0.0029 0.0003 0.0004 0 0.0029 0.0001 – – – – – – 0.0013 0.0001 0.0003 0.0002 0.0003 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.0589 1.2622 2.8097 2.6321 4.1188 2.8222 3.669 3.4453 2.5852 3.2278
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1028 1232 244 221 1888 1411 1722 125 2916 2643 1966 346

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 657.32 616.98 848.58 826.52 693.87 667.30 840.63 837.64 680.98 643.84 841.62 830.54
Standard Deviation 71.16 90.99 95.80 123.09 74.37 88.82 100.91 98.29 75.31 93.28 100.33 114.88
Intensity Change Log 2 — -0.091372 0.368455 0.330454 — -0.056330 0.276806 0.271665 — -0.073271 0.322118 0.300564

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000175 0.000364 0.000286 0.034198 0.000161 0.000755 0.000162 0.001243 0.000166 0.000572 0.000178 0.022292
Bud Neck 0.006506 0.040215 0.002020 0.011977 0.021091 0.042603 0.003366 0.001439 0.015949 0.041490 0.003199 0.008170
Bud Site 0.003891 0.016741 0.006291 0.047706 0.012373 0.018653 0.007700 0.009656 0.009383 0.017762 0.007525 0.033960
Cell Periphery 0.000152 0.000146 0.000249 0.000813 0.000269 0.000151 0.000105 0.000140 0.000228 0.000149 0.000123 0.000570
Cytoplasm 0.565168* 0.368736* 0.286772* 0.293804* 0.507203* 0.413635* 0.473158* 0.493489* 0.527638* 0.392706* 0.450025* 0.365945*
Cytoplasmic Foci 0.173780 0.262098* 0.033712 0.064021 0.180274* 0.263864* 0.006676 0.058201 0.177985 0.263041* 0.010032 0.061918
Eisosomes 0.000140 0.000133 0.000116 0.000150 0.000200 0.000204 0.000023 0.000087 0.000179 0.000171 0.000035 0.000127
Endoplasmic Reticulum 0.000633 0.000775 0.003515 0.004808 0.000701 0.000573 0.001664 0.001394 0.000677 0.000667 0.001894 0.003575
Endosome 0.007091 0.044393 0.005370 0.037146 0.008700 0.027605 0.001273 0.005369 0.008133 0.035430 0.001782 0.025666
Golgi 0.001946 0.006140 0.000117 0.032830 0.001539 0.008011 0.000072 0.000113 0.001682 0.007139 0.000078 0.021010
Lipid Particles 0.006803 0.012744 0.005009 0.004389 0.008330 0.008939 0.000678 0.001913 0.007792 0.010713 0.001216 0.003494
Mitochondria 0.003396 0.015417 0.000649 0.006968 0.002593 0.012118 0.001321 0.000297 0.002876 0.013656 0.001238 0.004558
Mitotic Spindle 0.001723 0.003475 0.004734 0.061458 0.001630 0.005345 0.006810 0.005748 0.001663 0.004473 0.006552 0.041332
None 0.005059 0.010488 0.006419 0.004556 0.002974 0.002180 0.007113 0.006313 0.003709 0.006053 0.007027 0.005191
Nuclear Periphery 0.000402 0.000627 0.001862 0.001918 0.000536 0.000350 0.001525 0.000959 0.000489 0.000479 0.001566 0.001572
Nuclear Periphery Foci 0.000579 0.000642 0.001589 0.005106 0.000227 0.000422 0.000592 0.001480 0.000351 0.000525 0.000715 0.003796
Nucleolus 0.001149 0.006183 0.001625 0.007034 0.001126 0.002785 0.000351 0.000426 0.001134 0.004369 0.000509 0.004646
Nucleus 0.181708* 0.098082 0.542807* 0.216912* 0.188356* 0.086187 0.444705* 0.244407* 0.186012* 0.091732 0.456880* 0.226845*
Peroxisomes 0.002807 0.020183 0.000682 0.004913 0.005432 0.024697 0.000124 0.002907 0.004506 0.022593 0.000193 0.004188
Vacuole 0.036411 0.088908 0.095803 0.156894 0.055699 0.078421 0.042263 0.164267 0.048899 0.083309 0.048908 0.159558
Vacuole Periphery 0.000479 0.003511 0.000373 0.002400 0.000587 0.002502 0.000319 0.000151 0.000549 0.002972 0.000326 0.001588

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.82 -1.93 -1.49 -1.45 -1.44 -3.06 -1.30 -1.27 -0.27 -1.21 -3.68 -1.41 -1.58 -1.44 -1.56
Bud Neck -13.06 4.64 -2.90 9.00 -5.11 -6.89 10.82 10.98 14.77 1.06 -12.02 11.22 4.39 14.82 -4.25
Bud Site -6.99 -0.74 -3.72 -1.00 -2.99 -2.60 3.68 -0.10 1.32 -1.90 -5.19 2.72 -2.58 -0.22 -3.83
Cell Periphery 0.58 -3.79 -1.02 -1.10 -0.07 3.39 5.28 4.03 1.09 -2.52 3.48 5.20 0.26 -0.97 -1.47
Cytoplasm 18.16 18.98 17.89 6.99 0.59 9.85 14.92 5.79 1.73 0.26 18.76 21.91 16.43 7.03 4.90
Cytoplasmic Foci -10.71 23.53 18.03 26.23 -0.42 -11.22 40.20 16.41 23.51 -5.19 -15.35 50.17 24.97 35.58 -6.14
Eisosomes 1.01 0.41 1.06 0.55 0.69 -0.04 14.71 5.86 3.55 -6.61 0.67 16.92 4.81 3.10 -6.82
Endoplasmic Reticulum -1.07 -7.93 -8.42 -8.29 0.15 1.11 -13.67 -5.04 -5.39 0.66 0.15 -16.18 -9.55 -9.65 -2.68
Endosome -13.26 1.33 -3.19 7.22 -3.59 -10.58 9.97 -0.20 6.22 -3.22 -16.64 11.64 -2.67 8.63 -5.94
Golgi -5.08 3.20 -1.48 -0.19 -2.09 -6.64 8.31 5.94 8.00 -3.55 -8.73 6.96 -1.33 1.26 -2.15
Lipid Particles -3.51 1.06 2.86 5.40 1.65 -0.52 10.15 7.45 6.32 -4.19 -2.78 11.42 6.39 7.56 -4.43
Mitochondria -6.03 2.53 1.08 6.81 -1.00 -6.95 3.70 6.06 9.01 2.37 -9.42 4.05 2.00 9.86 -0.54
Mitotic Spindle -1.91 -0.81 -5.06 -4.91 -4.79 -3.41 -3.75 -2.87 -2.49 -2.24 -3.78 -3.92 -5.77 -5.44 -5.09
None -3.10 -0.11 -1.03 1.82 -0.76 1.81 -5.31 -1.53 -2.17 0.53 -2.78 -4.05 -2.25 -0.04 -0.34
Nuclear Periphery -1.85 -11.55 -10.89 -9.81 -4.09 1.21 -12.63 -5.14 -7.44 4.83 0.07 -17.27 -10.97 -11.72 -1.78
Nuclear Periphery Foci -0.34 -4.43 -3.89 -3.87 -3.30 -3.12 -10.03 -2.79 -2.69 -2.44 -1.92 -7.12 -4.72 -4.63 -4.33
Nucleolus -4.67 -1.22 -1.60 0.08 -1.48 -2.81 3.46 -0.68 1.15 -1.78 -5.39 3.94 -1.67 -0.00 -2.03
Nucleus 12.29 -18.98 -4.16 -8.58 10.70 19.51 -38.46 -2.50 -7.57 12.24 22.51 -43.74 -4.76 -11.44 16.20
Peroxisomes -10.86 4.11 -0.32 9.56 -2.62 -10.73 8.55 1.95 10.30 -1.91 -14.75 9.79 1.79 14.29 -3.65
Vacuole -12.91 -8.47 -13.24 -10.28 -6.90 -6.60 -8.28 -9.01 -7.93 -7.41 -12.73 -12.25 -15.64 -13.12 -12.11
Vacuole Periphery -9.11 1.41 -0.65 6.98 -1.10 -4.87 4.02 5.35 6.08 0.42 -9.24 4.38 0.48 8.30 -1.57
Standard name
Human Ortholog
Description Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (15%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gal3

Gal3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gal3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available