Standard name
Human Ortholog
Description Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.41 0.39 0.3 0.27 0.37 0.42 0.48 0.46 0.44 0.51 0.37 0.45 0.34 0.41 0.39 0.33 0.59 0.32 0.18 0.19 0.19 0.25 0.22 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.29 0.06 0.11 0.2 0.17 0.37 0.24 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.29 0.18 0.47 0.38 0.28 0.16 0.23 0.19 0.13 0.13 0.13 0.11 0.22 0.23 0.25 0.13 0.15 0.08 0.61 0.57 0.59 0.46 0.44 0.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.6 0.66 0.43 0.42 0.45 0.29 0.37 0.36 0.38 0.33 0.32 0.32 0.54 0.46 0.46 0.57 0.39 0.69 0.06 0.11 0.08 0.12 0.13 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud 0 0 0 0 0 5 0 0 5 2 14 4 0 0 0 0 0 0 10 12 5 10 15 19
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 5
Bud Site 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 1 0 1 4 0 3 3 7 0 0 0 2 0 1 0 0 0 0 0 0
Cytoplasm 104 116 66 71 82 84 145 167 166 235 154 220 81 131 131 40 62 25 50 61 49 85 80 64
Endoplasmic Reticulum 5 0 1 0 0 0 0 0 1 0 1 0 2 0 0 4 2 5 1 0 1 3 1 3
Endosome 2 2 5 7 6 0 3 7 9 6 17 4 7 2 12 6 4 3 8 7 7 15 17 18
Golgi 1 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 2 4 1 1 2 2
Mitochondria 1 3 0 0 2 57 18 40 75 78 153 118 0 2 0 8 4 1 3 0 4 0 3 4
Nucleus 75 54 105 100 61 31 70 68 49 59 54 52 54 74 84 16 16 6 173 183 151 157 159 132
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 1 2 2 4
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 2 3 2 5 7 5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 6 8 11 14 16
Vac/Vac Membrane 152 196 96 109 98 58 113 131 145 151 132 158 129 145 154 70 41 53 16 35 20 42 48 44
Unique Cell Count 255 299 223 261 220 199 302 365 379 460 417 487 241 317 337 122 105 77 284 320 257 343 362 332
Labelled Cell Count 341 371 273 288 251 235 350 419 451 535 530 564 273 355 381 146 129 95 284 320 257 343 362 332


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.3 8.7 8.5 8.2 8.5 5.4 7.2 6.7 6.2 6.2 6.0 6.4 8.3 8.3 8.5 11.4 10.9 11.0 8.1 8.2 8.8
Std Deviation (1e-4) 1.2 1.3 1.1 1.6 2.1 1.4 1.7 1.2 1.3 1.0 1.0 1.1 1.3 1.4 1.4 2.4 2.5 3.0 1.2 1.4 2.5
Intensity Change (Log2) – – – -0.06 0.01 -0.66 -0.24 -0.35 -0.44 -0.44 -0.51 -0.42 -0.03 -0.04 -0.0 0.42 0.36 0.37 -0.07 -0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 1.7 2.7 4.3 3.9 3.5 5.3 1.9 3.9 0.9 2.8 2.3 0.6 5.1 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.3 0.3 0 0 0 0.1 0 1.2 0 0.4 0 0.9 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 8.6 3.7 5.1 7.1 6.5 10.4 8.1 0 0 0 0 0 0
Nucleus -1.9 -4.2 -6.9 -5.7 -7.3 -9.3 -9.8 -9.5 -10.8 -5.6 -5.8 -5.4 -6.3 -5.6 -6.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 0.3 -3.0 -1.3 -1.7 -1.2 -2.6 -2.9 -2.7 2.3 0.6 0.6 2.5 -0.7 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2181 2.9683 2.5022 2.55 2.314 2.6836 3.8042 5.5575 4.8928 4.3071 4.1667 4.6126 6.0239 7.1769 6.2936 6.6708 5.6855 6.6632
Actin 0.0277 0.0001 0.0488 0.012 0.0524 0.0004 0.0324 0.0001 0.0193 0.0006 0 0.0005 0.007 0.0001 0.0026 0.0296 0 0.0088
Bud 0.0017 0.0001 0.0008 0.0004 0.0007 0.0005 0.0014 0.0004 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0003
Bud Neck 0.0012 0.0003 0.0037 0.0011 0.0017 0.0024 0.0063 0.0003 0.0005 0.0012 0.0007 0.0016 0.0004 0.0003 0.0011 0.0004 0.0005 0.0033
Bud Periphery 0.0015 0 0.0009 0.0004 0.0014 0.0009 0.0039 0.0001 0.0004 0.0003 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0002
Bud Site 0.0026 0.0005 0.005 0.0051 0.0013 0.0003 0.0025 0.0007 0.0013 0.0084 0.0002 0.0002 0.0004 0.0005 0.0017 0.0002 0.0001 0.0003
Cell Periphery 0.0002 0 0.0003 0.0002 0.0003 0 0.0008 0 0.0001 0.0001 0 0 0 0 0.0001 0 0 0
Cytoplasm 0.0448 0.0611 0.0645 0.0502 0.0231 0.0335 0.0543 0.0604 0.0309 0.0186 0.0147 0.0249 0.0385 0.1043 0.0352 0.0208 0.0195 0.048
Cytoplasmic Foci 0.0245 0.0003 0.0065 0.0081 0.0066 0.0002 0.0129 0.0008 0.0146 0.0097 0.0001 0.0009 0.0024 0.0008 0.0046 0.0007 0.0001 0.0012
Eisosomes 0.0002 0 0.0002 0.0001 0.0006 0 0.0003 0 0.0002 0 0 0 0.0001 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0121 0.0007 0.0049 0.0032 0.0042 0.0008 0.0147 0.0006 0.0041 0.0005 0.0004 0.0007 0.0028 0.003 0.0005 0.0021 0.0003 0.0009
Endosome 0.0381 0.0004 0.0201 0.0175 0.074 0.001 0.0418 0.0005 0.0179 0.007 0.0004 0.0015 0.0059 0.0017 0.0019 0.0022 0.0002 0.0014
Golgi 0.0086 0 0.0145 0.0258 0.0372 0.0001 0.0152 0 0.0049 0.0023 0 0.0003 0.002 0.0001 0.0011 0.0011 0 0.0003
Lipid Particles 0.0139 0 0.0021 0.0066 0.0351 0 0.0127 0 0.0013 0.0112 0 0.0007 0.0075 0.0001 0.0039 0.0006 0 0.0001
Mitochondria 0.009 0.0002 0.0052 0.01 0.0414 0.0008 0.0417 0.0003 0.0093 0.0017 0.0001 0.0046 0.0006 0.0002 0.0005 0.0003 0.0002 0.0004
None 0.0129 0.0033 0.0152 0.0025 0.0856 0.0004 0.0051 0.0005 0.002 0.0007 0.0003 0.0019 0.0115 0.0038 0.0093 0.0023 0.0004 0.0024
Nuclear Periphery 0.0567 0.0137 0.0143 0.0122 0.0248 0.008 0.0447 0.0099 0.0118 0.0066 0.0096 0.0108 0.0145 0.0084 0.0059 0.0088 0.0054 0.0076
Nucleolus 0.0027 0.0013 0.0015 0.0015 0.0012 0.003 0.0104 0.0016 0.001 0.0034 0.0018 0.0021 0.0012 0.0011 0.0031 0.0006 0.0013 0.0019
Nucleus 0.7045 0.9119 0.7735 0.8297 0.5861 0.9442 0.658 0.9145 0.8725 0.8748 0.9663 0.9407 0.8977 0.8709 0.9212 0.9267 0.9686 0.9138
Peroxisomes 0.011 0 0.0034 0.0015 0.0061 0 0.0095 0 0.0024 0.0035 0 0.0002 0.0018 0 0.0027 0.0004 0 0.0009
Punctate Nuclear 0.0148 0.005 0.0124 0.0098 0.0049 0.0018 0.0142 0.0082 0.003 0.0468 0.0049 0.007 0.0046 0.0035 0.0032 0.0024 0.003 0.0074
Vacuole 0.0059 0.0009 0.0017 0.0012 0.0065 0.0014 0.012 0.0008 0.0014 0.0017 0.0004 0.0008 0.0007 0.001 0.0009 0.0002 0.0003 0.0008
Vacuole Periphery 0.0053 0.0002 0.0007 0.0009 0.0046 0.0003 0.0052 0.0001 0.0008 0.0003 0.0001 0.0003 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9962 19.7557 13.6125 10.4237 9.2099 7.8884 9.2741 9.6298 10.8074 13.176
Translational Efficiency 1.019 0.6517 0.9042 1.0238 1.0832 1.3189 1.7924 1.1141 0.8211 1.1617

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
Localization
Cell Percentages nucleus (22%), cytoplasm (19%), mixed (57%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Das2

Das2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Das2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available