Standard name | |
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Description | Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 5 | 2 | 14 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 12 | 5 | 10 | 15 | 19 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 4 | 0 | 3 | 3 | 7 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 104 | 116 | 66 | 71 | 82 | 84 | 145 | 167 | 166 | 235 | 154 | 220 | 81 | 131 | 131 | 40 | 62 | 25 | 50 | 61 | 49 | 85 | 80 | 64 |
Endoplasmic Reticulum | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 4 | 2 | 5 | 1 | 0 | 1 | 3 | 1 | 3 |
Endosome | 2 | 2 | 5 | 7 | 6 | 0 | 3 | 7 | 9 | 6 | 17 | 4 | 7 | 2 | 12 | 6 | 4 | 3 | 8 | 7 | 7 | 15 | 17 | 18 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 1 | 2 | 2 |
Mitochondria | 1 | 3 | 0 | 0 | 2 | 57 | 18 | 40 | 75 | 78 | 153 | 118 | 0 | 2 | 0 | 8 | 4 | 1 | 3 | 0 | 4 | 0 | 3 | 4 |
Nucleus | 75 | 54 | 105 | 100 | 61 | 31 | 70 | 68 | 49 | 59 | 54 | 52 | 54 | 74 | 84 | 16 | 16 | 6 | 173 | 183 | 151 | 157 | 159 | 132 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 2 | 4 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 5 | 7 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 6 | 8 | 11 | 14 | 16 |
Vac/Vac Membrane | 152 | 196 | 96 | 109 | 98 | 58 | 113 | 131 | 145 | 151 | 132 | 158 | 129 | 145 | 154 | 70 | 41 | 53 | 16 | 35 | 20 | 42 | 48 | 44 |
Unique Cell Count | 255 | 299 | 223 | 261 | 220 | 199 | 302 | 365 | 379 | 460 | 417 | 487 | 241 | 317 | 337 | 122 | 105 | 77 | 284 | 320 | 257 | 343 | 362 | 332 |
Labelled Cell Count | 341 | 371 | 273 | 288 | 251 | 235 | 350 | 419 | 451 | 535 | 530 | 564 | 273 | 355 | 381 | 146 | 129 | 95 | 284 | 320 | 257 | 343 | 362 | 332 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 8.7 | 8.5 | 8.2 | 8.5 | 5.4 | 7.2 | 6.7 | 6.2 | 6.2 | 6.0 | 6.4 | 8.3 | 8.3 | 8.5 | 11.4 | 10.9 | 11.0 | 8.1 | 8.2 | 8.8 |
Std Deviation (1e-4) | 1.2 | 1.3 | 1.1 | 1.6 | 2.1 | 1.4 | 1.7 | 1.2 | 1.3 | 1.0 | 1.0 | 1.1 | 1.3 | 1.4 | 1.4 | 2.4 | 2.5 | 3.0 | 1.2 | 1.4 | 2.5 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.01 | -0.66 | -0.24 | -0.35 | -0.44 | -0.44 | -0.51 | -0.42 | -0.03 | -0.04 | -0.0 | 0.42 | 0.36 | 0.37 | -0.07 | -0.04 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 1.7 | 2.7 | 4.3 | 3.9 | 3.5 | 5.3 | 1.9 | 3.9 | 0.9 | 2.8 | 2.3 | 0.6 | 5.1 | 0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.3 | 0.3 | 0 | 0 | 0 | 0.1 | 0 | 1.2 | 0 | 0.4 | 0 | 0.9 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 8.6 | 3.7 | 5.1 | 7.1 | 6.5 | 10.4 | 8.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.9 | -4.2 | -6.9 | -5.7 | -7.3 | -9.3 | -9.8 | -9.5 | -10.8 | -5.6 | -5.8 | -5.4 | -6.3 | -5.6 | -6.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.3 | 0.3 | -3.0 | -1.3 | -1.7 | -1.2 | -2.6 | -2.9 | -2.7 | 2.3 | 0.6 | 0.6 | 2.5 | -0.7 | 3.9 |