Standard name
Description Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; 18S rRNA biogenesis defect of the null mutant is functionally complemented by human EIF4A3

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0.11 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.17 0 0.11 0.15 0.36 0.43 0.25 0.39 0.51 0.54 0.53 0.06 0 0 0 0 0 0 0 0
Nucleus 0.42 0.64 0.41 0.31 0.61 0.56 0.44 0.45 0.49 0.44 0.46 0.42 0.46 0.44 0.53 0.21 0.44 0.23 0.36 0.26 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.67 0.47 0.6 0.71 0.3 0.25 0.34 0.34 0.35 0.32 0.37 0.32 0.61 0.47 0.29 0.63 0.43 0.6 0.46 0.51 0.32
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.07 0.13 0 0 0 0.06 0.06 0 0 0 0 0.12 0 0.09 0 0.07 0.06 0.11 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 1 8 8 6 9 12 10 0 0 0 1 2 3 1 0 4
Bud Neck 0 0 0 3 0 0 2 2 1 2 2 3 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 5 3 19 9 13 15 20 26 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 4 17 32 24 43 41 12 15 13 13 4 20 28 0 0 1 1 0 3
Endoplasmic Reticulum 0 0 2 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 24 11 9 19 19 35 13 13 1 7 0 5 4 0 3 14 1 3 11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0
Mitochondria 9 23 16 46 84 230 433 204 250 396 324 409 6 1 1 0 1 1 0 1 1
Nucleus 14 84 174 135 333 360 438 370 316 335 273 322 43 76 85 12 63 104 27 33 39
Nuclear Periphery 0 2 9 1 3 7 14 9 11 10 10 9 0 0 0 0 0 2 0 0 1
Nucleolus 22 62 254 311 163 159 343 280 225 248 221 246 57 82 46 37 61 273 35 64 48
Peroxisomes 0 0 1 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 28 58 6 13 38 48 36 27 19 30 2 21 7 5 6 33 4 14 13
Vac/Vac Membrane 0 0 4 2 21 28 25 26 24 24 9 16 0 4 7 2 2 11 2 8 24
Unique Cell Count 33 132 422 436 542 643 1001 815 646 769 597 771 93 174 160 60 143 456 76 127 151
Labelled Cell Count 45 171 516 585 658 844 1383 1033 909 1095 904 1091 112 209 178 60 143 456 76 127 151


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.8 7.0 6.3 5.9 5.0 4.7 4.8 4.6 4.4 4.2 4.3 7.2 8.4 7.8 8.1 8.7 9.1
Std Deviation (1e-4) 1.0 1.2 1.2 1.4 1.5 1.3 1.4 1.5 1.1 1.1 1.4 0.8 1.2 1.7 1.7 1.2 1.4 1.5
Intensity Change (Log2) -0.13 -0.25 -0.48 -0.57 -0.53 -0.58 -0.67 -0.73 -0.71 0.05 0.27 0.17 0.22 0.33 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.8 4.0 2.8 3.2 3.6 1.2 1.3 1.5 1.0 0 6.0 7.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.3 -3.4 -2.2 0 0 0 0 0 0 0 -1.5 -1.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.8 5.9 12.1 0 0 0 0 0 0 0 0 0
Nucleus -3.1 6.2 4.7 0.9 1.4 2.5 0.8 1.4 0.2 0.9 0.6 2.6
Nuclear Periphery 0 -2.2 -1.4 0 0 0 0 0 0 0 0 0
Nucleolus 3.4 -9.4 -11.6 0 0 0 0 0 0 0.2 -2.9 -6.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 3.3 -4.6 -3.8 0 0 0 0 0 0 -1.7 2.2 -1.0
Vacuole 0 2.8 3.2 1.9 2.4 2.8 2.4 0.8 1.5 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fal1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available