Standard name
Human Ortholog
Description Autophagy-specific protein required for autophagosome formation; forms a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; constitutively phosphorylated, and phosphorylation of residue S174 is required for function; high-copy suppressor of CIK1 deletion

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.98 0.99 0.9 0.8 0.72 0.68 0.69 0.62 0.58 0.54 0.59 0.95 0.97 0.92 0.85 0.89 0.9 0.71 0.85 0.76 0.78 0.76 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0.1 0.06 0.09 0.09 0.1 0.05 0 0 0.05 0 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.16 0 0.1 0.18 0.2 0.36 0.34 0 0 0 0.07 0 0.06 0.15 0.06 0.11 0.13 0.08 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.08 0.15 0.12 0.25 0.17 0.15 0.2 0.12 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 2 3 0 3 0 4 0 4 0 0 0 2 1 2 4 2 2 1 4 2
Bud 1 1 0 1 5 9 5 18 9 23 17 16 2 1 1 0 0 1 1 0 1 0 6 6
Bud Neck 0 2 0 8 2 9 8 21 6 10 0 5 2 2 1 1 2 0 1 2 1 1 2 2
Bud Site 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0
Cell Periphery 1 1 3 5 1 4 0 1 0 0 3 2 0 1 1 1 3 0 0 0 1 3 0 1
Cytoplasm 225 189 153 213 202 214 249 313 200 216 140 237 205 341 336 128 73 65 177 173 126 173 278 207
Endoplasmic Reticulum 0 1 1 1 0 3 0 2 5 1 1 4 1 0 1 15 1 2 0 0 1 0 5 1
Endosome 3 0 0 1 17 5 38 27 28 33 26 20 4 4 17 5 2 3 11 3 2 2 11 6
Golgi 2 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 1 1
Mitochondria 10 5 0 0 0 48 13 43 59 76 93 137 1 1 1 10 1 4 36 11 18 28 27 19
Nucleus 0 0 0 1 0 0 1 3 3 2 0 0 1 0 2 2 2 1 0 1 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 1 0 0 0 0 0 5 7 7 8 5 5 1 0 1 0 0 0 0 0 0 0 1 0
SpindlePole 3 0 1 18 38 35 91 77 49 76 30 36 3 5 16 2 2 1 4 0 3 4 7 6
Vac/Vac Membrane 1 0 0 1 3 4 9 9 5 11 4 8 1 0 0 5 0 1 3 2 1 3 8 5
Unique Cell Count 243 193 155 237 254 297 366 451 321 371 260 400 216 352 364 150 82 72 251 203 167 222 364 266
Labelled Cell Count 247 199 158 251 271 335 420 524 372 463 319 474 221 355 377 173 87 80 251 203 167 222 364 266


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.9 5.0 5.0 5.4 3.7 4.5 4.3 4.2 4.2 3.9 3.9 4.3 4.6 4.8 5.6 5.6 5.6 3.8 4.2 4.3
Std Deviation (1e-4) 0.7 0.7 1.7 2.9 1.8 0.9 1.3 1.2 1.9 1.4 0.7 0.9 1.2 1.5 1.5 1.5 1.6 1.6 0.8 1.7 1.4
Intensity Change (Log2) -0.02 0.11 -0.44 -0.16 -0.23 -0.27 -0.25 -0.36 -0.38 -0.22 -0.12 -0.07 0.15 0.16 0.16 -0.41 -0.27 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.6166 -1.0803 -1.0724 -1.3319 -0.6298 -1.1572 -0.197 0.1708 0.0861 -0.1145 0.0019 -0.0116 1.5356 2.158 1.9279 1.1851 1.0472 1.7367
Actin 0.0676 0.0001 0.0025 0 0.0459 0.0008 0.0244 0.0015 0.0133 0.0087 0.0155 0.0123 0.0791 0.0015 0.0168 0.0344 0.0125 0.0004
Bud 0.0009 0.0001 0.0076 0 0.0015 0.0002 0.0012 0.0112 0.001 0.0031 0.0036 0.0007 0.0013 0.0009 0.003 0.0003 0.0003 0.0001
Bud Neck 0.0037 0 0.0003 0 0.0011 0.0001 0.0044 0.0004 0.0015 0.0004 0.0007 0.0013 0.0019 0.0019 0.0028 0.0431 0.0002 0.0005
Bud Periphery 0.0011 0.0001 0.0077 0 0.0024 0.0006 0.0027 0.0048 0.0014 0.0077 0.0084 0.0028 0.0037 0.0019 0.0022 0.001 0.0004 0.0004
Bud Site 0.0055 0.0006 0.002 0 0.0014 0.0003 0.0147 0.02 0.0016 0.0024 0.005 0.0003 0.0036 0.0365 0.003 0.0009 0.0002 0.0001
Cell Periphery 0.0003 0 0.0005 0 0.0001 0.0001 0.0008 0.0004 0.0001 0.0002 0.0004 0.0001 0.0004 0.001 0.0003 0.0005 0 0.0001
Cytoplasm 0.0329 0.057 0.0279 0.0131 0.0244 0.0558 0.0895 0.2121 0.1598 0.1254 0.155 0.2079 0.0466 0.0796 0.0661 0.0778 0.112 0.0995
Cytoplasmic Foci 0.0312 0.0197 0.0127 0.0039 0.005 0.011 0.0267 0.0238 0.0178 0.0327 0.0179 0.0321 0.0464 0.0047 0.0218 0.0197 0.0362 0.0207
Eisosomes 0.0005 0.0001 0.0003 0 0.0001 0.0001 0.0006 0.0001 0.0002 0.0002 0.0004 0.0002 0.0009 0.0007 0.0003 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0045 0.0005 0.0006 0.0004 0.0025 0.0015 0.008 0.0023 0.0018 0.0013 0.0209 0.0013 0.0054 0.0011 0.0011 0.0005 0.001 0.0005
Endosome 0.04 0.0039 0.0075 0.0016 0.0081 0.0064 0.0252 0.0077 0.0169 0.0203 0.04 0.0096 0.0148 0.0004 0.0039 0.0013 0.0086 0.0045
Golgi 0.0108 0.0002 0.0006 0 0.0048 0.0011 0.0052 0.0004 0.0024 0.0028 0.021 0.0017 0.0089 0.0001 0.0019 0.002 0.0018 0.0006
Lipid Particles 0.0156 0.0014 0.0007 0.002 0.003 0.0017 0.0059 0.0007 0.0046 0.0057 0.0285 0.0034 0.0239 0.0003 0.013 0.0032 0.0086 0.0052
Mitochondria 0.0051 0.0002 0.0036 0.0001 0.0019 0.0036 0.0084 0.0006 0.001 0.0444 0.0238 0.0027 0.0033 0.0003 0.0013 0.0007 0.0009 0.0046
None 0.7354 0.9144 0.9163 0.97 0.8844 0.9124 0.7547 0.7075 0.7661 0.73 0.6066 0.711 0.7189 0.8658 0.8449 0.7991 0.7956 0.8603
Nuclear Periphery 0.0094 0.0002 0.0021 0.0034 0.0066 0.0008 0.0098 0.0008 0.0015 0.001 0.0251 0.005 0.0111 0.0003 0.0016 0.0003 0.0017 0.0001
Nucleolus 0.0007 0.0001 0.0004 0.0003 0.0002 0.0002 0.0009 0.0004 0.0002 0.0008 0.0017 0.0002 0.0006 0.0003 0.0008 0.0003 0.0004 0.0001
Nucleus 0.0039 0.0002 0.0035 0.0009 0.001 0.0007 0.0026 0.0015 0.0031 0.0023 0.0074 0.0014 0.0052 0.0008 0.0011 0.0005 0.0006 0.0002
Peroxisomes 0.0141 0.0003 0.0003 0.0001 0.0011 0.0008 0.0045 0.0004 0.0025 0.0067 0.0043 0.0013 0.0165 0.0002 0.0081 0.0112 0.0093 0.0016
Punctate Nuclear 0.0116 0.0004 0.001 0.0036 0.0032 0.0011 0.0043 0.0016 0.0012 0.0016 0.006 0.0031 0.0033 0.0012 0.0051 0.0021 0.0086 0.0005
Vacuole 0.0037 0.0005 0.0015 0.0003 0.0007 0.0006 0.0033 0.0017 0.0017 0.0015 0.004 0.001 0.003 0.0004 0.0007 0.0006 0.0006 0.0002
Vacuole Periphery 0.0014 0.0001 0.0004 0.0002 0.0005 0.0002 0.0021 0.0002 0.0004 0.0007 0.0036 0.0005 0.0012 0 0.0002 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.6675 10.014 11.1462 10.0641 3.631 9.3299 8.0157 12.5286 14.8632 13.2015
Translational Efficiency 1.5445 0.8586 0.6122 0.7151 2.2245 1.2938 1.8046 1.1347 1.035 1.3827

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1010 1131 284 1623 1832 1756 137 1370 2842 2887 421 2993

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 595.37 666.99 911.33 766.62 643.72 725.62 846.67 841.97 626.54 702.65 890.29 801.11
Standard Deviation 64.07 85.50 110.25 93.06 66.89 84.13 100.56 115.02 69.85 89.38 111.40 110.28
Intensity Change Log 2 0.163879 0.614187 0.364725 0.172781 0.395366 0.387336 0.168510 0.504654 0.376516

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000182 0.000338 0.001584 0.001080 0.000115 0.000219 0.000259 0.001838 0.000139 0.000266 0.001153 0.001427
Bud Neck 0.035225 0.026969 0.004264 0.005620 0.023743 0.045592 0.001678 0.010047 0.027824 0.038296 0.003423 0.007646
Bud Site 0.006663 0.014085 0.005938 0.013263 0.005013 0.017358 0.002582 0.033276 0.005600 0.016076 0.004846 0.022424
Cell Periphery 0.000391 0.000144 0.000618 0.000147 0.000143 0.000231 0.000312 0.000144 0.000231 0.000197 0.000518 0.000145
Cytoplasm 0.342338* 0.415819* 0.319380* 0.481746* 0.463711* 0.422357* 0.385764* 0.510943* 0.420577* 0.419796* 0.340982* 0.495110*
Cytoplasmic Foci 0.375985* 0.269199* 0.036717 0.044410 0.265138* 0.195365* 0.104528 0.026260 0.304531* 0.224290* 0.058784 0.036102
Eisosomes 0.000508 0.000131 0.000117 0.000061 0.000298 0.000162 0.000164 0.000041 0.000373 0.000150 0.000132 0.000052
Endoplasmic Reticulum 0.000798 0.000882 0.006653 0.001597 0.000693 0.000627 0.003801 0.002359 0.000730 0.000727 0.005725 0.001946
Endosome 0.015273 0.032428 0.015883 0.009908 0.013209 0.017030 0.017140 0.006877 0.013942 0.023062 0.016292 0.008521
Golgi 0.009179 0.004781 0.002168 0.001731 0.003736 0.003350 0.000175 0.003415 0.005670 0.003911 0.001520 0.002502
Lipid Particles 0.027841 0.009782 0.005071 0.002213 0.012554 0.009562 0.007021 0.001258 0.017987 0.009648 0.005705 0.001776
Mitochondria 0.032201 0.007837 0.000602 0.002086 0.005256 0.010408 0.000256 0.002679 0.014832 0.009401 0.000490 0.002357
Mitotic Spindle 0.001101 0.004702 0.003353 0.020158 0.001002 0.006349 0.000127 0.033168 0.001037 0.005704 0.002303 0.026114
None 0.004042 0.005806 0.003122 0.005330 0.002807 0.008069 0.003786 0.006734 0.003246 0.007183 0.003338 0.005973
Nuclear Periphery 0.000493 0.000773 0.002561 0.001941 0.000452 0.000459 0.001440 0.001260 0.000466 0.000582 0.002197 0.001629
Nuclear Periphery Foci 0.000179 0.000774 0.004672 0.001075 0.000226 0.000276 0.003639 0.002034 0.000209 0.000471 0.004336 0.001514
Nucleolus 0.001271 0.002764 0.001220 0.000823 0.001198 0.002677 0.001119 0.000437 0.001224 0.002711 0.001187 0.000646
Nucleus 0.102219 0.105715 0.458596* 0.272338* 0.153019* 0.179981* 0.310378* 0.248357* 0.134965* 0.150887* 0.410364* 0.261361*
Peroxisomes 0.021703 0.012540 0.003961 0.001211 0.005917 0.012372 0.001956 0.001404 0.011527 0.012437 0.003309 0.001300
Vacuole 0.020043 0.081214 0.123153 0.128865 0.040790 0.065957 0.153572 0.106808 0.033417 0.071934 0.133052 0.118769
Vacuole Periphery 0.002365 0.003318 0.000365 0.004397 0.000980 0.001599 0.000302 0.000661 0.001472 0.002272 0.000344 0.002687

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.85 -2.30 -1.44 -0.85 1.34 -3.50 -4.47 -1.61 -1.40 -1.05 -3.39 -2.62 -2.18 -1.71 0.69
Bud Neck 2.44 8.07 9.54 9.20 0.24 -7.51 13.92 8.91 14.31 -6.73 -4.68 11.56 13.30 17.08 -1.31
Bud Site -4.45 -1.03 -1.57 2.54 -0.42 -7.12 1.37 -6.10 -0.58 -6.67 -8.44 -1.11 -6.00 1.47 -4.39
Cell Periphery 5.06 -0.27 5.99 1.98 1.71 -4.90 -6.08 3.09 6.28 7.08 1.66 -1.24 6.68 6.50 2.13
Cytoplasm -6.13 4.69 -5.53 1.23 -8.38 4.75 5.71 6.86 2.41 -2.67 0.26 10.09 3.04 2.68 -8.39
Cytoplasmic Foci 10.63 40.69 44.80 32.28 -0.58 9.88 16.76 45.91 33.30 6.64 13.55 41.77 61.39 45.10 4.63
Eisosomes 12.32 13.07 17.14 3.65 6.42 3.51 3.14 6.29 13.89 8.92 8.12 7.96 11.74 10.65 10.04
Endoplasmic Reticulum -0.64 -10.38 -9.02 -9.71 8.69 0.78 -6.73 -11.26 -11.73 2.52 0.08 -11.87 -14.87 -15.55 8.67
Endosome -7.14 -0.22 5.10 11.39 3.26 -2.88 -0.29 7.95 10.63 2.73 -7.29 -0.65 8.67 14.91 4.47
Golgi 5.05 6.54 10.29 6.84 0.37 0.72 8.95 3.15 2.82 -1.62 3.87 6.43 9.05 6.46 -0.22
Lipid Particles 9.21 11.92 14.54 8.31 6.48 2.35 5.06 11.70 8.64 7.68 7.69 12.09 18.21 11.58 9.69
Mitochondria 8.58 11.77 11.45 6.43 -2.26 -4.41 9.13 5.78 8.18 -2.83 4.30 13.71 12.60 10.44 -3.41
Mitotic Spindle -2.58 -1.68 -6.42 -4.12 -4.30 -4.19 -0.43 -7.48 -5.30 -6.74 -4.89 -1.74 -9.82 -6.60 -7.37
None -1.40 1.54 0.21 1.70 -1.55 -4.08 -0.33 -4.56 2.30 -2.69 -4.31 0.74 -2.44 2.97 -3.15
Nuclear Periphery -0.85 -11.66 -14.16 -6.92 1.33 -0.09 -7.86 -13.52 -16.84 -5.06 -0.80 -13.08 -18.96 -14.60 0.17
Nuclear Periphery Foci -5.11 -6.56 -8.51 -4.09 5.33 -1.27 -8.53 -7.10 -6.89 2.69 -4.99 -7.83 -10.48 -8.57 5.58
Nucleolus -4.24 -1.06 0.10 4.15 1.08 -3.63 0.78 1.19 5.13 0.74 -5.21 -0.95 0.73 6.29 1.27
Nucleus -0.62 -17.61 -24.52 -23.59 7.79 -4.37 -7.50 -17.41 -12.78 1.10 -3.55 -17.69 -28.11 -23.22 7.19
Peroxisomes 4.75 9.44 12.54 10.93 2.13 -5.53 6.93 10.67 10.50 1.46 -0.91 8.94 15.69 14.63 2.44
Vacuole -17.61 -13.63 -33.70 -20.19 -2.06 -8.26 -8.82 -25.25 -20.47 0.06 -16.61 -15.53 -40.55 -29.48 -1.59
Vacuole Periphery -2.02 7.51 0.46 1.46 -2.04 -3.89 7.18 6.13 9.36 -0.88 -3.99 9.76 0.55 2.21 -2.06