Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; N-terminally propionylated in vivo; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 0.99 0.99 1.0 0.98 0.98 0.97 0.95 0.84 0.84 0.75 0.77 1.0 1.0 1.0 0.96 0.95 0.96 0.9 0.93 0.93 0.95 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.06 0.32 0.22 0.38 0.3 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 2 2 0 1 0 0 1 0 1 0 1 0 0 0
Bud 0 1 0 0 1 1 0 1 9 8 3 3 0 0 0 0 0 0 0 0 1 2 3 7
Bud Neck 0 0 1 0 1 3 2 1 2 3 1 0 0 0 1 0 1 0 1 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 3 2 0 0 1 3 3 2 2 2 1 0 2 4 8 4 0 0 0 0 0 0
Cytoplasm 157 211 290 221 199 151 207 216 284 309 190 213 350 445 479 417 217 229 203 208 264 312 399 436
Endoplasmic Reticulum 1 1 0 0 1 1 0 0 1 0 2 3 1 0 0 24 11 3 4 0 1 1 1 2
Endosome 1 0 1 0 0 0 1 0 0 4 1 0 0 1 0 5 0 0 2 1 2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 0 0
Mitochondria 4 5 0 0 4 4 3 13 109 81 96 84 0 0 0 23 7 3 4 5 6 2 2 1
Nucleus 12 0 0 1 3 1 0 2 2 2 3 5 0 0 1 1 1 5 0 0 0 1 0 0
Nuclear Periphery 5 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 2 2 2 0 1 0 0 0 0 0 0 2 0 0 0 3 4
Vac/Vac Membrane 0 0 3 0 1 0 1 3 4 3 3 1 0 0 1 7 6 7 1 1 1 2 3 4
Unique Cell Count 174 214 292 221 204 154 213 227 339 369 254 277 351 447 480 435 229 238 225 223 286 330 420 464
Labelled Cell Count 182 219 299 224 211 162 216 242 416 415 304 315 352 448 484 481 252 252 225 223 286 330 420 464


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 3.9 5.6 5.4 4.6 3.9 4.5 4.6 3.7 4.3 4.3 4.1 5.9 5.8 5.0 5.5 7.8 7.5 6.4 6.6 6.6
Std Deviation (1e-4) 0.6 0.5 1.0 0.9 1.5 0.9 1.4 1.4 1.0 1.4 1.4 0.9 1.4 1.2 1.0 1.2 1.7 1.6 1.6 1.7 1.7
Intensity Change (Log2) – – – -0.05 -0.28 -0.52 -0.32 -0.3 -0.61 -0.39 -0.37 -0.46 0.08 0.04 -0.16 -0.03 0.48 0.42 0.2 0.25 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2084 4.0417 3.4505 3.6522 3.3148 3.5702 7.8588 8.8879 8.8794 7.9456 7.8187 8.7517 4.7849 5.268 4.7099 5.047 5.0877 5.1435
Actin 0.0015 0.0002 0.0012 0.0017 0.0004 0.0005 0.0101 0.0003 0.0007 0.0009 0.0004 0.0008 0.0313 0.0039 0.0172 0.006 0.017 0.0079
Bud 0.0002 0.0004 0.0004 0.0005 0.0001 0.0002 0.0003 0.0004 0.0003 0.0003 0.0002 0.0009 0.0005 0.0005 0.0017 0.002 0.0002 0.0002
Bud Neck 0.0003 0.0004 0.0006 0.0012 0.0007 0.0024 0.001 0.0008 0.0005 0.0005 0.0009 0.0011 0.0152 0.0016 0.0006 0.0017 0.001 0.0018
Bud Periphery 0.0001 0.0001 0.0002 0.0003 0 0.0002 0.0002 0.0001 0.0002 0.0001 0.0001 0.0009 0.0006 0.0002 0.0019 0.0011 0.0002 0.0001
Bud Site 0.0008 0.0016 0.0059 0.0008 0.0001 0.0001 0.0012 0.0074 0.0007 0.0002 0.0004 0.0001 0.0032 0.0038 0.0011 0.0123 0.0006 0.0002
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0 0 0.0004 0.0001 0.0002 0.0002 0.0001 0
Cytoplasm 0.8568 0.8911 0.8541 0.8312 0.8828 0.8926 0.8829 0.933 0.8879 0.9333 0.88 0.9073 0.6978 0.8502 0.849 0.9048 0.6626 0.8951
Cytoplasmic Foci 0.0083 0.0065 0.01 0.0205 0.0259 0.0095 0.0121 0.006 0.0061 0.0035 0.0073 0.0049 0.0427 0.0157 0.0102 0.0121 0.0178 0.0101
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0004 0 0.0009 0 0.0005 0.0001
Endoplasmic Reticulum 0.0041 0.0015 0.0045 0.0033 0.0023 0.0025 0.0028 0.0013 0.0024 0.0025 0.0026 0.0014 0.0078 0.0021 0.0052 0.0062 0.0049 0.0036
Endosome 0.0049 0.003 0.005 0.0065 0.0088 0.007 0.0057 0.0011 0.0043 0.0013 0.0022 0.0014 0.0321 0.0042 0.0112 0.0164 0.0313 0.0127
Golgi 0.0003 0.0002 0.0003 0.0004 0.0006 0.0002 0.0015 0.0002 0.0003 0.0001 0.0001 0.0001 0.0105 0.0018 0.0018 0.0078 0.0063 0.0027
Lipid Particles 0.0002 0 0.0002 0.0003 0.0009 0 0.0006 0 0.0002 0 0.0001 0.0001 0.0199 0.0011 0.0017 0.0002 0.0397 0.002
Mitochondria 0.0003 0.0003 0.0006 0.0006 0.0003 0.0014 0.0005 0.0002 0.0003 0.0001 0.0003 0.0002 0.0046 0.0005 0.0022 0.0008 0.0095 0.0014
None 0.1019 0.0792 0.0903 0.0815 0.0598 0.0561 0.0625 0.0409 0.0753 0.0501 0.093 0.069 0.0877 0.0935 0.0622 0.0123 0.1363 0.0412
Nuclear Periphery 0.003 0.0014 0.0029 0.0041 0.0024 0.0026 0.003 0.0006 0.002 0.0012 0.0018 0.0008 0.0106 0.0011 0.01 0.002 0.0082 0.0062
Nucleolus 0.0002 0.0001 0.0002 0.0003 0 0.0001 0.0001 0.0001 0.0001 0 0.0002 0 0.0009 0.0003 0.0004 0.0002 0.0011 0.0001
Nucleus 0.0121 0.0117 0.0176 0.0325 0.0121 0.0197 0.0102 0.0058 0.0138 0.0043 0.0067 0.009 0.0064 0.0057 0.0112 0.0106 0.0259 0.0065
Peroxisomes 0.0002 0.0002 0.0005 0.001 0.0003 0.0001 0.0013 0.0001 0.0003 0.0001 0.0001 0.0001 0.0185 0.0086 0.0027 0.0003 0.0071 0.0045
Punctate Nuclear 0.0026 0.0008 0.0037 0.011 0.0014 0.0026 0.003 0.0011 0.0037 0.0008 0.0029 0.0015 0.0051 0.0039 0.0071 0.001 0.0269 0.0023
Vacuole 0.0018 0.0012 0.0014 0.0016 0.0008 0.0016 0.0007 0.0004 0.0006 0.0005 0.0007 0.0003 0.0025 0.0009 0.001 0.0016 0.0017 0.0008
Vacuole Periphery 0.0002 0.0002 0.0003 0.0004 0.0002 0.0004 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0013 0.0001 0.0004 0.0003 0.001 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1819.0946 1777.9902 1833.7192 2127.847 2024.4224 1503.1709 1808.8407 1668.5247 1573.6893 1804.0893
Translational Efficiency 1.5851 1.7008 1.7592 1.0024 1.6503 2.0701 1.4444 1.5741 1.582 1.6193

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
153 961 109 1664 66 1749 197 1731 219 2710 306 3395

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 787.69 853.93 1129.83 1086.51 860.36 956.49 1115.99 1048.95 809.59 920.12 1120.92 1067.36
Standard Deviation 115.50 163.45 149.09 195.45 121.41 127.52 176.77 185.81 121.96 149.59 167.57 191.52
Intensity Change Log 2 — 0.116490 0.520406 0.464002 — 0.152809 0.375312 0.285934 — 0.135564 0.446482 0.373787

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000364 0.000541 0.000648 0.005049 0.000173 0.000436 0.000363 0.000930 0.000307 0.000473 0.000464 0.002949
Bud Neck 0.005680 0.008619 0.002214 0.011753 0.002831 0.011331 0.002352 0.002796 0.004821 0.010369 0.002303 0.007186
Bud Site 0.013897 0.018377 0.005088 0.033881 0.005104 0.013044 0.005986 0.021078 0.011247 0.014935 0.005666 0.027353
Cell Periphery 0.000242 0.000138 0.000218 0.000095 0.000142 0.000101 0.000365 0.000089 0.000212 0.000114 0.000312 0.000092
Cytoplasm 0.607259* 0.622963* 0.453093* 0.601157* 0.567337* 0.677496* 0.417667* 0.711174* 0.595228* 0.658158* 0.430286* 0.657251*
Cytoplasmic Foci 0.213019* 0.122079 0.016116 0.012151 0.179210 0.089017 0.036769 0.012918 0.202830* 0.100741 0.029412 0.012542
Eisosomes 0.000116 0.000050 0.000047 0.000021 0.000090 0.000041 0.000094 0.000013 0.000109 0.000044 0.000077 0.000017
Endoplasmic Reticulum 0.001872 0.002295 0.006152 0.000999 0.001265 0.000523 0.002963 0.000526 0.001689 0.001151 0.004099 0.000758
Endosome 0.008750 0.010215 0.006576 0.008622 0.014861 0.007526 0.016294 0.004754 0.010592 0.008480 0.012832 0.006650
Golgi 0.002459 0.002346 0.000215 0.004187 0.000324 0.001627 0.002204 0.006575 0.001816 0.001882 0.001495 0.005405
Lipid Particles 0.002654 0.002283 0.001766 0.000810 0.003364 0.001390 0.002755 0.000434 0.002868 0.001707 0.002403 0.000618
Mitochondria 0.000641 0.002572 0.000357 0.010272 0.000137 0.002551 0.000264 0.003231 0.000489 0.002559 0.000297 0.006682
Mitotic Spindle 0.000266 0.009353 0.002668 0.109884* 0.000150 0.013040 0.015248 0.035834 0.000231 0.011733 0.010767 0.072128
None 0.001970 0.005163 0.002170 0.006030 0.000671 0.004472 0.003874 0.011631 0.001579 0.004717 0.003267 0.008886
Nuclear Periphery 0.000155 0.000435 0.001613 0.000767 0.000568 0.000360 0.001177 0.000530 0.000279 0.000386 0.001332 0.000646
Nuclear Periphery Foci 0.001755 0.002008 0.005506 0.001230 0.002705 0.000442 0.005912 0.001454 0.002041 0.000997 0.005767 0.001344
Nucleolus 0.000381 0.001186 0.000216 0.000486 0.000333 0.000533 0.000516 0.000177 0.000367 0.000764 0.000409 0.000329
Nucleus 0.087436 0.107300 0.352387* 0.098102 0.088998 0.096722 0.203339* 0.106589 0.087907 0.100473 0.256431* 0.102429
Peroxisomes 0.002326 0.004229 0.000221 0.003445 0.000955 0.002329 0.000317 0.001170 0.001913 0.003003 0.000283 0.002285
Vacuole 0.048007 0.076553 0.142507 0.087298 0.130365 0.075948 0.278026* 0.073544 0.072827 0.076162 0.229753* 0.080285
Vacuole Periphery 0.000752 0.001294 0.000223 0.003763 0.000417 0.001071 0.003515 0.004554 0.000651 0.001150 0.002343 0.004167

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.62 -4.23 -1.81 -1.39 -0.53 -1.03 -4.85 -1.36 -0.01 0.64 -1.02 -4.28 -1.99 -1.22 -0.51
Bud Neck -2.18 1.46 0.60 4.03 -1.57 -6.77 -0.54 0.11 7.83 1.35 -4.89 1.58 1.12 8.28 -1.09
Bud Site -0.85 1.55 0.45 2.87 -3.17 -4.19 -1.77 -3.36 0.86 -2.36 -1.03 1.29 -0.07 2.14 -3.92
Cell Periphery 4.09 -0.58 6.77 6.60 7.49 1.42 -2.07 3.19 1.01 3.97 4.23 -0.99 7.38 2.36 6.17
Cytoplasm -0.20 8.01 4.43 8.56 -6.26 -4.52 4.79 -2.27 8.23 -13.34 -3.85 10.45 1.40 13.25 -13.75
Cytoplasmic Foci 5.88 13.33 13.72 23.07 1.92 3.98 7.41 8.10 24.95 4.89 8.05 14.87 15.81 33.93 5.35
Eisosomes 2.23 1.65 3.25 9.85 6.90 3.95 0.77 5.67 9.79 10.72 3.15 1.51 4.35 13.35 12.58
Endoplasmic Reticulum -0.98 -5.71 1.87 4.96 8.03 3.47 -3.36 2.95 -2.41 7.26 1.50 -5.72 2.67 3.30 10.50
Endosome -0.51 1.16 3.15 7.67 2.85 2.50 1.96 4.68 7.57 2.21 1.16 1.57 4.97 10.65 2.97
Golgi 0.17 1.71 1.29 2.59 -1.85 -4.03 -0.74 -2.17 -0.35 -1.34 -0.00 1.33 0.50 1.05 -1.55
Lipid Particles 0.60 0.30 3.22 5.25 5.33 2.82 0.94 3.76 3.83 7.45 2.84 0.59 4.96 5.82 8.80
Mitochondria -2.80 0.73 -2.17 0.43 -2.51 -4.26 -1.12 -2.50 1.97 -2.34 -4.55 1.15 -2.82 1.57 -3.46
Mitotic Spindle -4.66 -2.61 -11.75 -8.14 -7.65 -3.10 -0.86 -5.87 -3.33 -3.71 -6.33 -2.18 -13.81 -8.05 -7.52
None -2.76 -0.11 -9.45 -2.66 -10.82 -3.42 -2.88 -8.62 -3.69 -5.35 -3.94 -2.13 -11.70 -4.65 -8.98
Nuclear Periphery -8.15 -6.86 -19.93 -13.93 3.37 2.38 -0.88 0.78 -9.27 5.29 0.96 -7.58 -4.85 -15.07 5.50
Nuclear Periphery Foci -0.87 -5.01 1.40 4.09 6.18 2.73 -2.35 1.59 -6.49 6.02 2.65 -5.76 1.95 -2.31 8.56
Nucleolus -3.65 0.84 0.29 3.82 -0.70 -1.12 0.52 1.91 5.23 2.03 -3.86 0.74 1.61 5.32 0.86
Nucleus -1.92 -10.09 -6.71 -8.53 7.79 0.88 -4.13 -2.73 -13.54 3.06 -0.75 -10.42 -7.12 -16.24 7.33
Peroxisomes -1.89 2.70 1.97 5.51 -2.23 -3.91 3.75 1.97 5.24 -0.83 -1.73 3.06 2.37 7.44 -2.23
Vacuole -4.43 -8.59 -18.72 -19.04 -0.24 3.57 -7.63 -0.63 -14.46 10.08 -0.22 -13.87 -12.18 -24.18 7.91
Vacuole Periphery -1.62 2.60 0.25 1.79 -2.05 -2.84 -0.74 -1.89 -0.49 -0.31 -2.23 -0.16 -1.19 0.38 -0.45