Standard name | |
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Description | RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 2 | 2 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 1 | 2 | 6 | 11 | – | 12 | 7 | 16 | 15 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 |
Bud Neck | 2 | 0 | 9 | 6 | 18 | 16 | 33 | – | 18 | 21 | 17 | 21 | 5 | 5 | 6 | 1 | 0 | 0 | 2 | 1 | 3 | 0 | 2 | 4 |
Bud Site | 3 | 0 | 0 | 3 | 4 | 4 | 11 | – | 14 | 12 | 25 | 17 | 0 | 0 | 0 | 0 | 1 | 2 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 9 | 6 | 12 | 18 | 20 | 25 | – | 16 | 18 | 17 | 21 | 16 | 54 | 71 | 15 | 8 | 15 | 1 | 3 | 0 | 2 | 2 | 4 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 5 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 26 | 20 | 19 | 14 | 13 | 15 | 12 | – | 6 | 5 | 3 | 15 | 11 | 28 | 59 | 54 | 40 | 59 | 36 | 18 | 31 | 6 | 21 | 28 |
Golgi | 0 | 2 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 |
Mitochondria | 49 | 9 | 2 | 8 | 5 | 32 | 14 | – | 69 | 40 | 179 | 81 | 2 | 0 | 1 | 17 | 11 | 12 | 8 | 4 | 3 | 0 | 4 | 5 |
Nucleus | 69 | 59 | 78 | 57 | 57 | 45 | 51 | – | 35 | 37 | 47 | 48 | 73 | 69 | 63 | 33 | 20 | 32 | 22 | 29 | 26 | 4 | 13 | 7 |
Nuclear Periphery | 6 | 1 | 0 | 0 | 0 | 1 | 1 | – | 1 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 2 | 1 |
Nucleolus | 125 | 68 | 25 | 73 | 100 | 121 | 95 | – | 81 | 80 | 93 | 75 | 68 | 131 | 77 | 49 | 35 | 47 | 104 | 66 | 89 | 33 | 66 | 54 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 2 | 0 | 4 | 2 | 0 | 3 | 3 | 0 | 1 | 7 | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 128 | 49 | 127 | 152 | 205 | 212 | 283 | – | 192 | 195 | 174 | 236 | 122 | 186 | 169 | 116 | 71 | 101 | 115 | 54 | 84 | 30 | 73 | 66 |
Vac/Vac Membrane | 2 | 3 | 2 | 1 | 3 | 0 | 0 | – | 0 | 0 | 2 | 0 | 2 | 2 | 6 | 6 | 4 | 7 | 8 | 5 | 12 | 3 | 10 | 9 |
Unique Cell Count | 274 | 154 | 203 | 219 | 284 | 324 | 373 | 292 | 287 | 363 | 355 | 203 | 316 | 310 | 185 | 127 | 170 | 319 | 192 | 265 | 86 | 204 | 192 | |
Labelled Cell Count | 421 | 222 | 268 | 328 | 426 | 478 | 538 | 447 | 420 | 577 | 531 | 301 | 478 | 455 | 299 | 195 | 287 | 319 | 192 | 265 | 86 | 204 | 192 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.1 | 5.5 | 5.7 | 5.6 | 5.9 | 4.7 | 4.7 | – | 4.1 | 4.3 | 3.6 | 4.0 | 6.0 | 5.7 | 5.5 | 6.4 | 6.3 | 6.3 | 5.0 | 5.6 | 5.9 |
Std Deviation (1e-4) | 0.5 | 0.8 | 0.9 | 1.5 | 1.2 | 0.9 | 1.1 | – | 1.4 | 1.1 | 0.8 | 1.1 | 1.0 | 1.0 | 1.3 | 1.3 | 1.0 | 1.2 | 1.0 | 1.2 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.04 | -0.28 | -0.27 | – | -0.47 | -0.4 | -0.66 | -0.5 | 0.06 | 0.01 | -0.04 | 0.17 | 0.15 | 0.15 | -0.19 | -0.04 | 0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | -0.9 | 0.9 | 0 | 0 | – | 0 | 0 | 0 | 0 | -1.1 | -2.0 | -1.6 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | 1.7 | 1.7 | 1.9 | – | 1.3 | 1.7 | 1.0 | 1.6 | 2.2 | 4.9 | 6.2 | 2.2 | 1.5 | 2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.1 | -2.1 | 0 | 0 | – | 0 | 0 | 0 | 0 | -1.5 | -0.2 | 3.0 | 5.0 | 5.1 | 6.0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.5 | 3.1 |
Nucleus | -2.7 | -4.5 | -6.5 | -6.8 | – | -6.9 | -6.6 | -7.0 | -6.8 | -0.5 | -4.1 | -4.5 | -4.5 | -4.4 | -4.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 5.1 | 5.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | 5.1 | 7.1 | 3.5 | 3.5 | 3.5 | 3.7 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.5 | 2.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | -0.5 | -0.8 | -1.8 | 0 | -1.2 | -0.6 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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