Standard name
Description RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0.05 0.09 – 0.06 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0 0.07 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.05 0.06 0.06 0.07 – 0.05 0.06 0.05 0.06 0.08 0.17 0.23 0.08 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0.13 0.09 0.06 0.05 0.05 0 – 0 0 0 0 0.05 0.09 0.19 0.29 0.31 0.35 0.11 0.09 0.12 0.08 0.11 0.15
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.06 0 0 0 0.1 0 – 0.24 0.14 0.49 0.23 0 0 0 0.09 0.09 0.07 0 0 0 0 0 0
Nucleus 0.25 0.38 0.38 0.26 0.2 0.14 0.14 – 0.12 0.13 0.13 0.14 0.36 0.22 0.2 0.18 0.16 0.19 0.07 0.15 0.1 0.05 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.46 0.44 0.12 0.33 0.35 0.37 0.25 – 0.28 0.28 0.26 0.21 0.33 0.41 0.25 0.26 0.28 0.28 0.33 0.35 0.34 0.39 0.32 0.28
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.47 0.32 0.63 0.69 0.72 0.65 0.76 – 0.66 0.68 0.48 0.66 0.6 0.59 0.55 0.63 0.56 0.59 0.36 0.28 0.32 0.36 0.36 0.35
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 2 – 1 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 1 0 0 1 2 6 11 – 12 7 16 15 0 0 0 1 0 0 1 0 1 0 0 1
Bud Neck 2 0 9 6 18 16 33 – 18 21 17 21 5 5 6 1 0 0 2 1 3 0 2 4
Bud Site 3 0 0 3 4 4 11 – 14 12 25 17 0 0 0 0 1 2 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 9 9 6 12 18 20 25 – 16 18 17 21 16 54 71 15 8 15 1 3 0 2 2 4
Endoplasmic Reticulum 0 2 0 0 0 2 0 – 0 0 0 0 0 0 0 5 3 5 0 0 0 0 1 1
Endosome 26 20 19 14 13 15 12 – 6 5 3 15 11 28 59 54 40 59 36 18 31 6 21 28
Golgi 0 2 0 1 0 0 0 – 0 0 0 0 0 0 0 1 0 0 2 2 1 0 0 0
Mitochondria 49 9 2 8 5 32 14 – 69 40 179 81 2 0 1 17 11 12 8 4 3 0 4 5
Nucleus 69 59 78 57 57 45 51 – 35 37 47 48 73 69 63 33 20 32 22 29 26 4 13 7
Nuclear Periphery 6 1 0 0 0 1 1 – 1 3 0 0 2 0 0 0 0 0 3 1 0 0 2 1
Nucleolus 125 68 25 73 100 121 95 – 81 80 93 75 68 131 77 49 35 47 104 66 89 33 66 54
Peroxisomes 0 0 0 0 0 2 0 – 2 0 4 2 0 3 3 0 1 7 1 0 1 0 0 0
SpindlePole 128 49 127 152 205 212 283 – 192 195 174 236 122 186 169 116 71 101 115 54 84 30 73 66
Vac/Vac Membrane 2 3 2 1 3 0 0 – 0 0 2 0 2 2 6 6 4 7 8 5 12 3 10 9
Unique Cell Count 274 154 203 219 284 324 373 292 287 363 355 203 316 310 185 127 170 319 192 265 86 204 192
Labelled Cell Count 421 222 268 328 426 478 538 447 420 577 531 301 478 455 299 195 287 319 192 265 86 204 192


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.5 5.7 5.6 5.9 4.7 4.7 – 4.1 4.3 3.6 4.0 6.0 5.7 5.5 6.4 6.3 6.3 5.0 5.6 5.9
Std Deviation (1e-4) 0.5 0.8 0.9 1.5 1.2 0.9 1.1 – 1.4 1.1 0.8 1.1 1.0 1.0 1.3 1.3 1.0 1.2 1.0 1.2 1.3
Intensity Change (Log2) – – – -0.01 0.04 -0.28 -0.27 – -0.47 -0.4 -0.66 -0.5 0.06 0.01 -0.04 0.17 0.15 0.15 -0.19 -0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck -0.9 0.9 0 0 – 0 0 0 0 -1.1 -2.0 -1.6 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.7 1.7 1.9 – 1.3 1.7 1.0 1.6 2.2 4.9 6.2 2.2 1.5 2.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -1.1 -2.1 0 0 – 0 0 0 0 -1.5 -0.2 3.0 5.0 5.1 6.0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 3.7 3.5 3.1
Nucleus -2.7 -4.5 -6.5 -6.8 – -6.9 -6.6 -7.0 -6.8 -0.5 -4.1 -4.5 -4.5 -4.4 -4.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.1 5.7 0 0 – 0 0 0 0 5.1 7.1 3.5 3.5 3.5 3.7
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.5 2.2 0 0 – 0 0 0 0 -0.5 -0.8 -1.8 0 -1.2 -0.6
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nsi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available