Standard name | |
---|---|
Human Ortholog | |
Description | 3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 6 | 3 | 1 | 1 | 1 | 2 | 1 | 2 | 4 | 1 | 1 | 2 | 2 | 2 | 39 | 49 | 51 | 5 | 25 | 9 | 6 | 19 | 16 |
Bud | 4 | 4 | 11 | 28 | 4 | 13 | 14 | 21 | 11 | 13 | 13 | 7 | 8 | 7 | 1 | 6 | 2 | 2 | 0 | 7 | 1 | 2 | 1 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 7 |
Bud Site | 0 | 1 | 2 | 5 | 1 | 4 | 6 | 6 | 5 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 22 | 7 | 4 | 7 | 2 | 1 | 1 | 3 | 4 | 1 | 4 | 30 | 15 | 22 | 26 | 28 | 14 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 1 | 8 | 7 | 4 | 7 | 8 | 4 | 9 | 4 | 8 | 8 | 10 | 15 | 13 | 10 | 16 | 17 | 20 | 0 | 1 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 1 | 5 | 2 | 2 | 4 | 6 | 0 | 4 | 4 | 7 | 4 | 11 | 12 | 11 | 7 | 8 | 18 | 10 | 0 | 2 | 0 | 1 | 2 | 1 |
Endosome | 0 | 25 | 8 | 9 | 13 | 9 | 13 | 12 | 4 | 4 | 0 | 1 | 31 | 37 | 42 | 2 | 1 | 2 | 12 | 44 | 19 | 32 | 37 | 33 |
Golgi | 1 | 35 | 5 | 3 | 9 | 2 | 2 | 2 | 2 | 6 | 5 | 2 | 155 | 202 | 153 | 225 | 291 | 235 | 15 | 74 | 26 | 41 | 63 | 32 |
Mitochondria | 167 | 416 | 321 | 233 | 255 | 362 | 399 | 462 | 380 | 384 | 300 | 340 | 468 | 489 | 452 | 37 | 73 | 22 | 120 | 373 | 223 | 295 | 354 | 336 |
Nucleus | 0 | 7 | 0 | 0 | 4 | 3 | 4 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 1 | 3 | 0 | 4 | 0 | 0 | 3 | 1 |
Nuclear Periphery | 9 | 13 | 13 | 6 | 11 | 23 | 33 | 28 | 36 | 36 | 19 | 36 | 9 | 11 | 6 | 2 | 3 | 0 | 1 | 3 | 2 | 4 | 8 | 6 |
Nucleolus | 12 | 13 | 9 | 10 | 14 | 26 | 44 | 53 | 36 | 42 | 16 | 22 | 7 | 3 | 4 | 14 | 11 | 12 | 0 | 4 | 1 | 2 | 1 | 1 |
Peroxisomes | 0 | 15 | 19 | 10 | 7 | 3 | 9 | 8 | 4 | 3 | 1 | 0 | 33 | 37 | 57 | 4 | 1 | 1 | 8 | 19 | 41 | 26 | 41 | 22 |
SpindlePole | 0 | 7 | 2 | 5 | 2 | 2 | 9 | 3 | 3 | 4 | 2 | 0 | 3 | 6 | 3 | 3 | 5 | 6 | 1 | 3 | 1 | 2 | 3 | 4 |
Vac/Vac Membrane | 17 | 103 | 12 | 6 | 42 | 18 | 21 | 39 | 14 | 19 | 7 | 15 | 76 | 55 | 68 | 62 | 79 | 68 | 4 | 39 | 2 | 7 | 15 | 43 |
Unique Cell Count | 175 | 525 | 339 | 249 | 298 | 390 | 447 | 529 | 404 | 414 | 315 | 369 | 591 | 640 | 616 | 321 | 370 | 323 | 171 | 608 | 334 | 429 | 561 | 521 |
Labelled Cell Count | 213 | 680 | 421 | 326 | 381 | 482 | 561 | 652 | 508 | 537 | 379 | 450 | 849 | 889 | 827 | 448 | 579 | 446 | 171 | 608 | 334 | 429 | 561 | 521 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 8.9 | 6.8 | 6.1 | 7.1 | 6.8 | 6.7 | 7.0 | 7.2 | 7.1 | 6.6 | 7.3 | 8.4 | 8.2 | 8.0 | 20.8 | 25.8 | 24.7 | 6.8 | 6.9 | 7.7 |
Std Deviation (1e-4) | 1.5 | 2.1 | 1.4 | 1.3 | 1.6 | 1.5 | 1.7 | 1.7 | 1.6 | 1.7 | 1.6 | 1.7 | 1.7 | 1.7 | 1.7 | 7.4 | 9.7 | 9.5 | 1.4 | 1.6 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.17 | 0.05 | -0.01 | -0.02 | 0.04 | 0.08 | 0.06 | -0.06 | 0.1 | 0.3 | 0.27 | 0.23 | 1.61 | 1.92 | 1.86 | -0.01 | 0.02 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.9 | 6.3 | 7.0 |
Bud | 3.9 | -1.6 | 0.1 | -0.1 | 0.6 | -0.4 | -0.1 | 0.6 | -1.1 | -2.0 | -2.4 | 0 | -1.1 | -2.7 | -2.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | -1.1 | 2.3 | 0.3 | 1.3 | 3.6 | 3.4 | 1.7 |
Cytoplasm | 0 | 0.2 | 0 | 0 | -0.4 | -1.2 | -0.1 | 0.4 | 0.6 | 0.5 | 0 | -0.5 | 2.0 | 1.9 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 1.7 | 0 | 0 | 0 | 3.4 | 2.4 |
Endosome | 0.9 | 1.4 | 0 | 0.5 | -0.1 | -1.5 | -1.5 | 0 | 0 | 2.1 | 2.4 | 3.0 | 0 | 0 | 0 |
Golgi | 0 | 1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9.6 | 11.0 | 9.3 | 18.5 | 20.8 | 19.1 |
Mitochondria | -0.6 | -3.9 | -1.0 | -2.7 | -3.6 | -0.4 | -1.1 | 0.3 | -1.4 | -6.3 | -7.2 | -8.0 | -21.4 | -20.1 | -22.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.0 | -0.1 | 1.3 | 2.1 | 1.0 | 2.8 | 2.7 | 1.3 | 3.1 | -2.2 | -2.0 | -3.0 | -2.8 | -2.7 | -3.6 |
Nucleolus | 0.9 | 1.4 | 2.5 | 4.0 | 4.1 | 3.6 | 4.1 | 1.6 | 2.1 | -1.7 | 0 | 0 | 1.2 | 0.3 | 0.8 |
Peroxisomes | -0.9 | -2.1 | -3.8 | -2.7 | -3.4 | -3.6 | -4.0 | -3.9 | -4.6 | 0 | 0.1 | 2.0 | -3.1 | -4.3 | -4.0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.8 | 4.8 | 0.7 | 0.8 | 2.3 | -0.1 | 0.7 | -1.0 | 0.4 | 4.7 | 3.0 | 4.0 | 6.4 | 7.1 | 6.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.0798 | 9.0604 | 9.3565 | 9.3571 | 8.4907 | 9.1194 | 9.7672 | 8.9394 | 8.9162 | 8.0154 | 7.7627 | 9.0742 | 9.1347 | 9.2459 | 9.736 | 10.1544 | 9.8682 | 9.8921 |
Actin | 0.0109 | 0.0162 | 0.0184 | 0.0008 | 0.0105 | 0.0064 | 0.0236 | 0.0222 | 0.0245 | 0.003 | 0.0422 | 0.0051 | 0.0048 | 0.0175 | 0.0053 | 0.0032 | 0.003 | 0.0057 |
Bud | 0.0003 | 0.0046 | 0.0011 | 0.0003 | 0.0004 | 0.0002 | 0.0007 | 0.0012 | 0.0008 | 0.0004 | 0.0019 | 0.0002 | 0.0004 | 0.0018 | 0.0003 | 0.0027 | 0.0003 | 0.0029 |
Bud Neck | 0.0003 | 0.0003 | 0.0009 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Bud Periphery | 0.0009 | 0.0024 | 0.002 | 0.0011 | 0.0006 | 0.0004 | 0.002 | 0.002 | 0.001 | 0.0009 | 0.0028 | 0.0004 | 0.0005 | 0.0033 | 0.0006 | 0.0061 | 0.0011 | 0.0007 |
Bud Site | 0.0011 | 0.0087 | 0.0084 | 0.0003 | 0.0009 | 0.0003 | 0.001 | 0.0015 | 0.0053 | 0.0007 | 0.0085 | 0.0002 | 0.0056 | 0.0044 | 0.0009 | 0.0028 | 0.0001 | 0.0003 |
Cell Periphery | 0.0005 | 0.0009 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0.0005 | 0.0002 | 0.0006 | 0.0001 | 0.0002 |
Cytoplasm | 0.0011 | 0.002 | 0.0008 | 0.0001 | 0.0024 | 0.0006 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.0005 | 0.0013 | 0.0013 | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0006 |
Cytoplasmic Foci | 0.0096 | 0.014 | 0.0131 | 0.0014 | 0.011 | 0.0067 | 0.0109 | 0.0151 | 0.0071 | 0.0052 | 0.0068 | 0.0132 | 0.0126 | 0.0124 | 0.0093 | 0.0112 | 0.0053 | 0.0053 |
Eisosomes | 0.0007 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0012 | 0.0006 | 0.0006 | 0.0001 | 0.0025 | 0.0004 | 0.0007 | 0.0002 | 0.0011 | 0.0002 | 0.0017 | 0.0003 | 0.0002 | 0.0005 | 0.0005 | 0.0001 | 0.0004 | 0.0024 |
Endosome | 0.0218 | 0.0355 | 0.0253 | 0.0063 | 0.0429 | 0.0176 | 0.0277 | 0.041 | 0.0239 | 0.0176 | 0.0431 | 0.0183 | 0.0314 | 0.0285 | 0.0274 | 0.0031 | 0.0351 | 0.0182 |
Golgi | 0.0433 | 0.0476 | 0.0314 | 0.0117 | 0.0917 | 0.0267 | 0.0479 | 0.077 | 0.0398 | 0.0259 | 0.07 | 0.0524 | 0.0549 | 0.0546 | 0.0565 | 0.0054 | 0.0792 | 0.0499 |
Lipid Particles | 0.0196 | 0.0146 | 0.0108 | 0.001 | 0.0074 | 0.0087 | 0.0285 | 0.0066 | 0.0107 | 0.0031 | 0.0128 | 0.0049 | 0.0216 | 0.0113 | 0.0063 | 0.0094 | 0.0023 | 0.0096 |
Mitochondria | 0.8548 | 0.816 | 0.8427 | 0.957 | 0.8067 | 0.9002 | 0.831 | 0.8077 | 0.8559 | 0.9343 | 0.7855 | 0.8915 | 0.8435 | 0.8421 | 0.867 | 0.9288 | 0.8448 | 0.8834 |
None | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0007 | 0.0001 | 0.0014 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0006 |
Nuclear Periphery | 0.0005 | 0.0005 | 0.0018 | 0 | 0.0009 | 0.0004 | 0.0022 | 0.0001 | 0.0004 | 0.0001 | 0.0026 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0018 |
Nucleolus | 0.0002 | 0.0009 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0007 | 0.0007 | 0.0004 | 0.004 | 0 | 0.0006 |
Nucleus | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0 | 0.0007 |
Peroxisomes | 0.0238 | 0.0155 | 0.0276 | 0.0133 | 0.0152 | 0.0259 | 0.0085 | 0.0197 | 0.0241 | 0.0043 | 0.0094 | 0.0085 | 0.0132 | 0.012 | 0.0181 | 0.004 | 0.0222 | 0.0132 |
Punctate Nuclear | 0.0018 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0049 | 0.0001 | 0.0002 | 0 | 0.001 | 0 | 0.0001 | 0.0001 | 0 | 0.0015 | 0 | 0.0001 |
Vacuole | 0.003 | 0.0107 | 0.0048 | 0.0024 | 0.0016 | 0.0011 | 0.0023 | 0.0014 | 0.0011 | 0.0017 | 0.0027 | 0.0005 | 0.0026 | 0.0034 | 0.0022 | 0.0116 | 0.0014 | 0.0011 |
Vacuole Periphery | 0.004 | 0.0077 | 0.0075 | 0.0031 | 0.0034 | 0.0031 | 0.0037 | 0.0025 | 0.0022 | 0.0014 | 0.0048 | 0.0022 | 0.0048 | 0.0047 | 0.0037 | 0.0031 | 0.0041 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.3019 | 22.3132 | 23.3751 | 32.582 | 21.8923 | 25.6805 | 35.9979 | 34.2609 | 30.8585 | 37.1568 |
Translational Efficiency | 1.1498 | 1.596 | 1.4538 | 0.9652 | 1.5343 | 1.6385 | 1.1677 | 1.1957 | 1.1795 | 1.1268 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
311 | 970 | 163 | 614 | 614 | 1665 | 1554 | 1006 | 925 | 2635 | 1717 | 1620 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 778.35 | 791.89 | 1239.09 | 1507.99 | 871.36 | 818.27 | 1204.39 | 1984.68 | 840.09 | 808.56 | 1207.68 | 1804.01 |
Standard Deviation | 100.33 | 145.30 | 215.48 | 275.83 | 125.43 | 126.47 | 220.82 | 414.32 | 125.53 | 134.32 | 220.55 | 434.65 |
Intensity Change Log 2 | — | 0.024881 | 0.670790 | 0.954136 | — | -0.090692 | 0.466962 | 1.187566 | — | -0.035008 | 0.566725 | 1.082118 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001613 | 0.005385 | 0.015241 | 0.022561 | 0.002710 | 0.004152 | 0.003406 | 0.002910 | 0.002341 | 0.004606 | 0.004530 | 0.010358 |
Bud Neck | 0.005013 | 0.003438 | 0.013650 | 0.002515 | 0.002182 | 0.001197 | 0.005950 | 0.000182 | 0.003134 | 0.002022 | 0.006681 | 0.001066 |
Bud Site | 0.013520 | 0.019916 | 0.046999 | 0.006984 | 0.004377 | 0.007064 | 0.024117 | 0.002537 | 0.007451 | 0.011795 | 0.026289 | 0.004222 |
Cell Periphery | 0.000348 | 0.000645 | 0.000401 | 0.000894 | 0.000200 | 0.000268 | 0.000532 | 0.001676 | 0.000250 | 0.000407 | 0.000519 | 0.001380 |
Cytoplasm | 0.008811 | 0.005590 | 0.003794 | 0.000872 | 0.001977 | 0.001971 | 0.003961 | 0.000642 | 0.004275 | 0.003303 | 0.003946 | 0.000729 |
Cytoplasmic Foci | 0.066102 | 0.046899 | 0.114210 | 0.013498 | 0.041757 | 0.036240 | 0.025756 | 0.003108 | 0.049942 | 0.040164 | 0.034154 | 0.007046 |
Eisosomes | 0.000125 | 0.000131 | 0.000146 | 0.000424 | 0.000061 | 0.000086 | 0.000102 | 0.000115 | 0.000082 | 0.000102 | 0.000106 | 0.000232 |
Endoplasmic Reticulum | 0.000596 | 0.000491 | 0.001224 | 0.000648 | 0.000357 | 0.000250 | 0.000666 | 0.000510 | 0.000437 | 0.000339 | 0.000719 | 0.000562 |
Endosome | 0.119196 | 0.096662 | 0.117253 | 0.013312 | 0.059993 | 0.076677 | 0.040795 | 0.007355 | 0.079898 | 0.084034 | 0.048054 | 0.009613 |
Golgi | 0.092100 | 0.103287 | 0.117070* | 0.121106* | 0.060063 | 0.098684 | 0.043247 | 0.037742 | 0.070834 | 0.100378 | 0.050255 | 0.069338 |
Lipid Particles | 0.010165 | 0.006609 | 0.004723 | 0.002207 | 0.008645 | 0.003513 | 0.006307 | 0.001754 | 0.009156 | 0.004653 | 0.006156 | 0.001926 |
Mitochondria | 0.561780* | 0.620398* | 0.293678* | 0.755255* | 0.757666* | 0.717873* | 0.760733* | 0.907591* | 0.691806* | 0.681990* | 0.716394* | 0.849854* |
Mitotic Spindle | 0.014070 | 0.010841 | 0.108303* | 0.007954 | 0.008209 | 0.007094 | 0.018492 | 0.000443 | 0.010180 | 0.008473 | 0.027018 | 0.003290 |
None | 0.000406 | 0.000372 | 0.000300 | 0.001140 | 0.000232 | 0.000191 | 0.000947 | 0.000930 | 0.000290 | 0.000258 | 0.000886 | 0.001009 |
Nuclear Periphery | 0.000254 | 0.000105 | 0.001604 | 0.000915 | 0.000144 | 0.000051 | 0.001070 | 0.000256 | 0.000181 | 0.000070 | 0.001120 | 0.000506 |
Nuclear Periphery Foci | 0.000469 | 0.000374 | 0.002559 | 0.000427 | 0.000259 | 0.000103 | 0.001437 | 0.000125 | 0.000329 | 0.000203 | 0.001544 | 0.000239 |
Nucleolus | 0.000743 | 0.000723 | 0.000945 | 0.000342 | 0.000187 | 0.000144 | 0.000855 | 0.000034 | 0.000374 | 0.000357 | 0.000863 | 0.000151 |
Nucleus | 0.001630 | 0.001670 | 0.000994 | 0.000122 | 0.000152 | 0.000094 | 0.000644 | 0.000019 | 0.000649 | 0.000674 | 0.000677 | 0.000058 |
Peroxisomes | 0.049593 | 0.055658 | 0.068507 | 0.006984 | 0.036822 | 0.027759 | 0.017766 | 0.001353 | 0.041116 | 0.038029 | 0.022583 | 0.003487 |
Vacuole | 0.027397 | 0.010418 | 0.063382 | 0.015136 | 0.005832 | 0.007925 | 0.023781 | 0.005911 | 0.013082 | 0.008843 | 0.027541 | 0.009407 |
Vacuole Periphery | 0.026069 | 0.010389 | 0.025018 | 0.026704 | 0.008174 | 0.008662 | 0.019435 | 0.024810 | 0.014191 | 0.009298 | 0.019965 | 0.025528 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.81 | -3.62 | -5.83 | -4.72 | -1.45 | -1.45 | -0.80 | -0.17 | 1.11 | 0.50 | -3.08 | -2.81 | -5.17 | -3.68 | -3.68 |
Bud Neck | 1.27 | -3.46 | 2.00 | 1.40 | 4.82 | 1.46 | -3.97 | 3.14 | 4.44 | 8.13 | 1.81 | -4.03 | 3.42 | 3.35 | 8.03 |
Bud Site | -1.79 | -4.12 | 2.32 | 4.40 | 5.00 | -1.98 | -7.33 | 1.39 | 3.71 | 8.19 | -2.80 | -7.27 | 2.46 | 5.66 | 8.88 |
Cell Periphery | -3.29 | -0.85 | -2.44 | -1.07 | -2.22 | -2.05 | -4.50 | -2.14 | -2.04 | -1.65 | -4.00 | -4.05 | -2.59 | -2.23 | -1.95 |
Cytoplasm | 1.09 | 1.76 | 2.86 | 3.85 | 4.97 | 0.02 | -2.07 | 1.72 | 3.82 | 4.73 | 0.86 | 0.23 | 3.31 | 5.12 | 5.12 |
Cytoplasmic Foci | 2.92 | -3.80 | 8.22 | 11.19 | 9.16 | 1.45 | 4.14 | 11.26 | 19.07 | 12.60 | 2.92 | 4.27 | 13.53 | 21.15 | 13.02 |
Eisosomes | -0.17 | -0.59 | -4.33 | -4.76 | -4.47 | -4.23 | -5.62 | -4.35 | -2.43 | -1.00 | -1.58 | -1.87 | -5.55 | -5.25 | -5.04 |
Endoplasmic Reticulum | 1.07 | -3.67 | -0.51 | -1.58 | 3.27 | 1.91 | -2.34 | -1.52 | -2.82 | 1.05 | 1.97 | -2.37 | -1.67 | -3.26 | 1.22 |
Endosome | 2.18 | 0.19 | 11.42 | 16.63 | 11.23 | -2.94 | 3.58 | 10.89 | 21.35 | 12.66 | -0.78 | 6.21 | 15.39 | 26.91 | 14.96 |
Golgi | -1.20 | -1.65 | -2.46 | -1.71 | -0.39 | -6.46 | 3.18 | 3.68 | 11.01 | 1.15 | -5.81 | 4.28 | 0.26 | 5.86 | -3.79 |
Lipid Particles | 1.00 | 1.46 | 2.37 | 3.41 | 1.62 | 2.70 | 1.23 | 3.91 | 2.22 | 5.43 | 2.58 | 1.71 | 4.45 | 3.94 | 5.63 |
Mitochondria | -2.48 | 8.03 | -7.87 | -7.59 | -15.67 | 2.65 | -0.13 | -10.41 | -18.26 | -13.81 | 0.72 | -1.64 | -11.94 | -17.64 | -12.27 |
Mitotic Spindle | 0.77 | -5.64 | 1.41 | 1.05 | 6.10 | 0.70 | -4.63 | 6.08 | 7.17 | 9.66 | 0.97 | -6.05 | 3.99 | 4.36 | 9.49 |
None | 0.36 | 1.39 | -7.09 | -8.48 | -11.10 | 0.51 | -3.69 | -7.79 | -8.72 | 0.10 | 0.52 | -3.41 | -10.58 | -11.97 | -0.68 |
Nuclear Periphery | 2.61 | -2.74 | -2.59 | -3.25 | 1.24 | 3.90 | -5.10 | -2.36 | -4.99 | 4.41 | 4.39 | -5.48 | -3.21 | -4.42 | 3.13 |
Nuclear Periphery Foci | 0.87 | -2.75 | 0.29 | -0.59 | 2.79 | 3.31 | -3.57 | 2.54 | -0.64 | 3.99 | 2.55 | -4.02 | 1.59 | -0.89 | 4.31 |
Nucleolus | 0.07 | -0.58 | 1.24 | 1.03 | 1.74 | 0.16 | -2.58 | 1.55 | 1.56 | 3.42 | 0.03 | -2.02 | 1.67 | 1.50 | 3.03 |
Nucleus | -0.02 | 0.87 | 2.48 | 1.66 | 1.71 | 1.28 | -2.43 | 3.53 | 3.82 | 3.13 | -0.05 | -0.08 | 2.81 | 1.80 | 2.98 |
Peroxisomes | -0.67 | -1.37 | 5.36 | 10.72 | 5.46 | 1.89 | 4.26 | 8.50 | 11.05 | 9.57 | 0.70 | 4.36 | 9.75 | 15.22 | 9.81 |
Vacuole | 4.51 | -3.65 | 2.53 | -2.16 | 4.88 | -1.84 | -9.57 | -0.06 | 1.70 | 9.39 | 2.80 | -6.47 | 1.81 | -0.80 | 8.48 |
Vacuole Periphery | 4.81 | 0.25 | -0.16 | -5.02 | -0.38 | -0.45 | -6.90 | -6.09 | -6.13 | -1.86 | 3.63 | -3.26 | -4.93 | -7.94 | -2.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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