Standard name
Human Ortholog
Description 3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.13 0.16 0 0 0 0 0 0
Bud 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.08 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0.07 0.07 0.06 0.08 0.07 0.06
Golgi 0 0.07 0 0 0 0 0 0 0 0 0 0 0.26 0.32 0.25 0.7 0.79 0.73 0.09 0.12 0.08 0.1 0.11 0.06
Mitochondria 0.95 0.79 0.95 0.94 0.86 0.93 0.89 0.87 0.94 0.93 0.95 0.92 0.79 0.76 0.73 0.12 0.2 0.07 0.7 0.61 0.67 0.69 0.63 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.06 0.07 0.05 0.09 0.09 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.05 0.07 0.1 0.1 0.09 0.1 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.06 0.09 0 0 0 0.05 0 0.12 0.06 0.07 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.2 0 0 0.14 0.05 0.05 0.07 0 0.05 0 0 0.13 0.09 0.11 0.19 0.21 0.21 0 0.06 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 3 1 1 1 2 1 2 4 1 1 2 2 2 39 49 51 5 25 9 6 19 16
Bud 4 4 11 28 4 13 14 21 11 13 13 7 8 7 1 6 2 2 0 7 1 2 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 7
Bud Site 0 1 2 5 1 4 6 6 5 3 1 1 0 0 0 0 0 0
Cell Periphery 1 22 7 4 7 2 1 1 3 4 1 4 30 15 22 26 28 14 0 0 1 0 0 1
Cytoplasm 1 8 7 4 7 8 4 9 4 8 8 10 15 13 10 16 17 20 0 1 0 0 1 1
Endoplasmic Reticulum 1 5 2 2 4 6 0 4 4 7 4 11 12 11 7 8 18 10 0 2 0 1 2 1
Endosome 0 25 8 9 13 9 13 12 4 4 0 1 31 37 42 2 1 2 12 44 19 32 37 33
Golgi 1 35 5 3 9 2 2 2 2 6 5 2 155 202 153 225 291 235 15 74 26 41 63 32
Mitochondria 167 416 321 233 255 362 399 462 380 384 300 340 468 489 452 37 73 22 120 373 223 295 354 336
Nucleus 0 7 0 0 4 3 4 3 0 0 1 0 0 1 0 4 1 3 0 4 0 0 3 1
Nuclear Periphery 9 13 13 6 11 23 33 28 36 36 19 36 9 11 6 2 3 0 1 3 2 4 8 6
Nucleolus 12 13 9 10 14 26 44 53 36 42 16 22 7 3 4 14 11 12 0 4 1 2 1 1
Peroxisomes 0 15 19 10 7 3 9 8 4 3 1 0 33 37 57 4 1 1 8 19 41 26 41 22
SpindlePole 0 7 2 5 2 2 9 3 3 4 2 0 3 6 3 3 5 6 1 3 1 2 3 4
Vac/Vac Membrane 17 103 12 6 42 18 21 39 14 19 7 15 76 55 68 62 79 68 4 39 2 7 15 43
Unique Cell Count 175 525 339 249 298 390 447 529 404 414 315 369 591 640 616 321 370 323 171 608 334 429 561 521
Labelled Cell Count 213 680 421 326 381 482 561 652 508 537 379 450 849 889 827 448 579 446 171 608 334 429 561 521


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 8.9 6.8 6.1 7.1 6.8 6.7 7.0 7.2 7.1 6.6 7.3 8.4 8.2 8.0 20.8 25.8 24.7 6.8 6.9 7.7
Std Deviation (1e-4) 1.5 2.1 1.4 1.3 1.6 1.5 1.7 1.7 1.6 1.7 1.6 1.7 1.7 1.7 1.7 7.4 9.7 9.5 1.4 1.6 1.7
Intensity Change (Log2) -0.17 0.05 -0.01 -0.02 0.04 0.08 0.06 -0.06 0.1 0.3 0.27 0.23 1.61 1.92 1.86 -0.01 0.02 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 5.9 6.3 7.0
Bud 3.9 -1.6 0.1 -0.1 0.6 -0.4 -0.1 0.6 -1.1 -2.0 -2.4 0 -1.1 -2.7 -2.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.2 0 0 0 0 0 0 -1.1 2.3 0.3 1.3 3.6 3.4 1.7
Cytoplasm 0 0.2 0 0 -0.4 -1.2 -0.1 0.4 0.6 0.5 0 -0.5 2.0 1.9 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2.4 1.7 0 0 0 3.4 2.4
Endosome 0.9 1.4 0 0.5 -0.1 -1.5 -1.5 0 0 2.1 2.4 3.0 0 0 0
Golgi 0 1.3 0 0 0 0 0 0 0 9.6 11.0 9.3 18.5 20.8 19.1
Mitochondria -0.6 -3.9 -1.0 -2.7 -3.6 -0.4 -1.1 0.3 -1.4 -6.3 -7.2 -8.0 -21.4 -20.1 -22.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.0 -0.1 1.3 2.1 1.0 2.8 2.7 1.3 3.1 -2.2 -2.0 -3.0 -2.8 -2.7 -3.6
Nucleolus 0.9 1.4 2.5 4.0 4.1 3.6 4.1 1.6 2.1 -1.7 0 0 1.2 0.3 0.8
Peroxisomes -0.9 -2.1 -3.8 -2.7 -3.4 -3.6 -4.0 -3.9 -4.6 0 0.1 2.0 -3.1 -4.3 -4.0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.8 4.8 0.7 0.8 2.3 -0.1 0.7 -1.0 0.4 4.7 3.0 4.0 6.4 7.1 6.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0798 9.0604 9.3565 9.3571 8.4907 9.1194 9.7672 8.9394 8.9162 8.0154 7.7627 9.0742 9.1347 9.2459 9.736 10.1544 9.8682 9.8921
Actin 0.0109 0.0162 0.0184 0.0008 0.0105 0.0064 0.0236 0.0222 0.0245 0.003 0.0422 0.0051 0.0048 0.0175 0.0053 0.0032 0.003 0.0057
Bud 0.0003 0.0046 0.0011 0.0003 0.0004 0.0002 0.0007 0.0012 0.0008 0.0004 0.0019 0.0002 0.0004 0.0018 0.0003 0.0027 0.0003 0.0029
Bud Neck 0.0003 0.0003 0.0009 0.0004 0.0002 0.0002 0.0003 0.0002 0.0003 0.0003 0.0005 0.0002 0.0004 0.0006 0.0002 0.0002 0.0001 0.0002
Bud Periphery 0.0009 0.0024 0.002 0.0011 0.0006 0.0004 0.002 0.002 0.001 0.0009 0.0028 0.0004 0.0005 0.0033 0.0006 0.0061 0.0011 0.0007
Bud Site 0.0011 0.0087 0.0084 0.0003 0.0009 0.0003 0.001 0.0015 0.0053 0.0007 0.0085 0.0002 0.0056 0.0044 0.0009 0.0028 0.0001 0.0003
Cell Periphery 0.0005 0.0009 0.0006 0.0004 0.0001 0.0002 0.0003 0.0002 0.0003 0.0003 0.0004 0.0001 0.0004 0.0005 0.0002 0.0006 0.0001 0.0002
Cytoplasm 0.0011 0.002 0.0008 0.0001 0.0024 0.0006 0.0006 0.0004 0.0006 0.0003 0.0005 0.0013 0.0013 0.0005 0.0003 0.0002 0.0001 0.0006
Cytoplasmic Foci 0.0096 0.014 0.0131 0.0014 0.011 0.0067 0.0109 0.0151 0.0071 0.0052 0.0068 0.0132 0.0126 0.0124 0.0093 0.0112 0.0053 0.0053
Eisosomes 0.0007 0.0004 0.0004 0.0001 0.0002 0.0002 0.0004 0.0004 0.0004 0.0001 0.0007 0.0002 0.0003 0.0004 0.0002 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0012 0.0006 0.0006 0.0001 0.0025 0.0004 0.0007 0.0002 0.0011 0.0002 0.0017 0.0003 0.0002 0.0005 0.0005 0.0001 0.0004 0.0024
Endosome 0.0218 0.0355 0.0253 0.0063 0.0429 0.0176 0.0277 0.041 0.0239 0.0176 0.0431 0.0183 0.0314 0.0285 0.0274 0.0031 0.0351 0.0182
Golgi 0.0433 0.0476 0.0314 0.0117 0.0917 0.0267 0.0479 0.077 0.0398 0.0259 0.07 0.0524 0.0549 0.0546 0.0565 0.0054 0.0792 0.0499
Lipid Particles 0.0196 0.0146 0.0108 0.001 0.0074 0.0087 0.0285 0.0066 0.0107 0.0031 0.0128 0.0049 0.0216 0.0113 0.0063 0.0094 0.0023 0.0096
Mitochondria 0.8548 0.816 0.8427 0.957 0.8067 0.9002 0.831 0.8077 0.8559 0.9343 0.7855 0.8915 0.8435 0.8421 0.867 0.9288 0.8448 0.8834
None 0.0005 0.0002 0.0005 0 0.0007 0.0001 0.0014 0.0001 0.0002 0.0001 0.0008 0.0001 0.0003 0.0002 0.0001 0.0007 0.0001 0.0006
Nuclear Periphery 0.0005 0.0005 0.0018 0 0.0009 0.0004 0.0022 0.0001 0.0004 0.0001 0.0026 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0018
Nucleolus 0.0002 0.0009 0.0005 0.0001 0.0001 0.0001 0.0008 0.0001 0.0002 0.0001 0.0006 0.0001 0.0007 0.0007 0.0004 0.004 0 0.0006
Nucleus 0.0003 0.0006 0.0004 0.0001 0.0002 0.0001 0.0007 0.0001 0.0001 0.0001 0.0007 0 0.0003 0.0003 0.0003 0.0007 0 0.0007
Peroxisomes 0.0238 0.0155 0.0276 0.0133 0.0152 0.0259 0.0085 0.0197 0.0241 0.0043 0.0094 0.0085 0.0132 0.012 0.0181 0.004 0.0222 0.0132
Punctate Nuclear 0.0018 0.0001 0.0002 0 0.0003 0.0001 0.0049 0.0001 0.0002 0 0.001 0 0.0001 0.0001 0 0.0015 0 0.0001
Vacuole 0.003 0.0107 0.0048 0.0024 0.0016 0.0011 0.0023 0.0014 0.0011 0.0017 0.0027 0.0005 0.0026 0.0034 0.0022 0.0116 0.0014 0.0011
Vacuole Periphery 0.004 0.0077 0.0075 0.0031 0.0034 0.0031 0.0037 0.0025 0.0022 0.0014 0.0048 0.0022 0.0048 0.0047 0.0037 0.0031 0.0041 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.3019 22.3132 23.3751 32.582 21.8923 25.6805 35.9979 34.2609 30.8585 37.1568
Translational Efficiency 1.1498 1.596 1.4538 0.9652 1.5343 1.6385 1.1677 1.1957 1.1795 1.1268

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
311 970 163 614 614 1665 1554 1006 925 2635 1717 1620

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 778.35 791.89 1239.09 1507.99 871.36 818.27 1204.39 1984.68 840.09 808.56 1207.68 1804.01
Standard Deviation 100.33 145.30 215.48 275.83 125.43 126.47 220.82 414.32 125.53 134.32 220.55 434.65
Intensity Change Log 2 0.024881 0.670790 0.954136 -0.090692 0.466962 1.187566 -0.035008 0.566725 1.082118

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001613 0.005385 0.015241 0.022561 0.002710 0.004152 0.003406 0.002910 0.002341 0.004606 0.004530 0.010358
Bud Neck 0.005013 0.003438 0.013650 0.002515 0.002182 0.001197 0.005950 0.000182 0.003134 0.002022 0.006681 0.001066
Bud Site 0.013520 0.019916 0.046999 0.006984 0.004377 0.007064 0.024117 0.002537 0.007451 0.011795 0.026289 0.004222
Cell Periphery 0.000348 0.000645 0.000401 0.000894 0.000200 0.000268 0.000532 0.001676 0.000250 0.000407 0.000519 0.001380
Cytoplasm 0.008811 0.005590 0.003794 0.000872 0.001977 0.001971 0.003961 0.000642 0.004275 0.003303 0.003946 0.000729
Cytoplasmic Foci 0.066102 0.046899 0.114210 0.013498 0.041757 0.036240 0.025756 0.003108 0.049942 0.040164 0.034154 0.007046
Eisosomes 0.000125 0.000131 0.000146 0.000424 0.000061 0.000086 0.000102 0.000115 0.000082 0.000102 0.000106 0.000232
Endoplasmic Reticulum 0.000596 0.000491 0.001224 0.000648 0.000357 0.000250 0.000666 0.000510 0.000437 0.000339 0.000719 0.000562
Endosome 0.119196 0.096662 0.117253 0.013312 0.059993 0.076677 0.040795 0.007355 0.079898 0.084034 0.048054 0.009613
Golgi 0.092100 0.103287 0.117070* 0.121106* 0.060063 0.098684 0.043247 0.037742 0.070834 0.100378 0.050255 0.069338
Lipid Particles 0.010165 0.006609 0.004723 0.002207 0.008645 0.003513 0.006307 0.001754 0.009156 0.004653 0.006156 0.001926
Mitochondria 0.561780* 0.620398* 0.293678* 0.755255* 0.757666* 0.717873* 0.760733* 0.907591* 0.691806* 0.681990* 0.716394* 0.849854*
Mitotic Spindle 0.014070 0.010841 0.108303* 0.007954 0.008209 0.007094 0.018492 0.000443 0.010180 0.008473 0.027018 0.003290
None 0.000406 0.000372 0.000300 0.001140 0.000232 0.000191 0.000947 0.000930 0.000290 0.000258 0.000886 0.001009
Nuclear Periphery 0.000254 0.000105 0.001604 0.000915 0.000144 0.000051 0.001070 0.000256 0.000181 0.000070 0.001120 0.000506
Nuclear Periphery Foci 0.000469 0.000374 0.002559 0.000427 0.000259 0.000103 0.001437 0.000125 0.000329 0.000203 0.001544 0.000239
Nucleolus 0.000743 0.000723 0.000945 0.000342 0.000187 0.000144 0.000855 0.000034 0.000374 0.000357 0.000863 0.000151
Nucleus 0.001630 0.001670 0.000994 0.000122 0.000152 0.000094 0.000644 0.000019 0.000649 0.000674 0.000677 0.000058
Peroxisomes 0.049593 0.055658 0.068507 0.006984 0.036822 0.027759 0.017766 0.001353 0.041116 0.038029 0.022583 0.003487
Vacuole 0.027397 0.010418 0.063382 0.015136 0.005832 0.007925 0.023781 0.005911 0.013082 0.008843 0.027541 0.009407
Vacuole Periphery 0.026069 0.010389 0.025018 0.026704 0.008174 0.008662 0.019435 0.024810 0.014191 0.009298 0.019965 0.025528

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.81 -3.62 -5.83 -4.72 -1.45 -1.45 -0.80 -0.17 1.11 0.50 -3.08 -2.81 -5.17 -3.68 -3.68
Bud Neck 1.27 -3.46 2.00 1.40 4.82 1.46 -3.97 3.14 4.44 8.13 1.81 -4.03 3.42 3.35 8.03
Bud Site -1.79 -4.12 2.32 4.40 5.00 -1.98 -7.33 1.39 3.71 8.19 -2.80 -7.27 2.46 5.66 8.88
Cell Periphery -3.29 -0.85 -2.44 -1.07 -2.22 -2.05 -4.50 -2.14 -2.04 -1.65 -4.00 -4.05 -2.59 -2.23 -1.95
Cytoplasm 1.09 1.76 2.86 3.85 4.97 0.02 -2.07 1.72 3.82 4.73 0.86 0.23 3.31 5.12 5.12
Cytoplasmic Foci 2.92 -3.80 8.22 11.19 9.16 1.45 4.14 11.26 19.07 12.60 2.92 4.27 13.53 21.15 13.02
Eisosomes -0.17 -0.59 -4.33 -4.76 -4.47 -4.23 -5.62 -4.35 -2.43 -1.00 -1.58 -1.87 -5.55 -5.25 -5.04
Endoplasmic Reticulum 1.07 -3.67 -0.51 -1.58 3.27 1.91 -2.34 -1.52 -2.82 1.05 1.97 -2.37 -1.67 -3.26 1.22
Endosome 2.18 0.19 11.42 16.63 11.23 -2.94 3.58 10.89 21.35 12.66 -0.78 6.21 15.39 26.91 14.96
Golgi -1.20 -1.65 -2.46 -1.71 -0.39 -6.46 3.18 3.68 11.01 1.15 -5.81 4.28 0.26 5.86 -3.79
Lipid Particles 1.00 1.46 2.37 3.41 1.62 2.70 1.23 3.91 2.22 5.43 2.58 1.71 4.45 3.94 5.63
Mitochondria -2.48 8.03 -7.87 -7.59 -15.67 2.65 -0.13 -10.41 -18.26 -13.81 0.72 -1.64 -11.94 -17.64 -12.27
Mitotic Spindle 0.77 -5.64 1.41 1.05 6.10 0.70 -4.63 6.08 7.17 9.66 0.97 -6.05 3.99 4.36 9.49
None 0.36 1.39 -7.09 -8.48 -11.10 0.51 -3.69 -7.79 -8.72 0.10 0.52 -3.41 -10.58 -11.97 -0.68
Nuclear Periphery 2.61 -2.74 -2.59 -3.25 1.24 3.90 -5.10 -2.36 -4.99 4.41 4.39 -5.48 -3.21 -4.42 3.13
Nuclear Periphery Foci 0.87 -2.75 0.29 -0.59 2.79 3.31 -3.57 2.54 -0.64 3.99 2.55 -4.02 1.59 -0.89 4.31
Nucleolus 0.07 -0.58 1.24 1.03 1.74 0.16 -2.58 1.55 1.56 3.42 0.03 -2.02 1.67 1.50 3.03
Nucleus -0.02 0.87 2.48 1.66 1.71 1.28 -2.43 3.53 3.82 3.13 -0.05 -0.08 2.81 1.80 2.98
Peroxisomes -0.67 -1.37 5.36 10.72 5.46 1.89 4.26 8.50 11.05 9.57 0.70 4.36 9.75 15.22 9.81
Vacuole 4.51 -3.65 2.53 -2.16 4.88 -1.84 -9.57 -0.06 1.70 9.39 2.80 -6.47 1.81 -0.80 8.48
Vacuole Periphery 4.81 0.25 -0.16 -5.02 -0.38 -0.45 -6.90 -6.09 -6.13 -1.86 3.63 -3.26 -4.93 -7.94 -2.39