Standard name
Human Ortholog
Description Coproporphyrinogen III oxidase; oxygen-requiring enzyme that catalyzes sixth step in heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p); human homolog CPOX can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.97 0.95 0.83 0.79 0.77 0.73 0.68 0.78 0.71 0.99 1.0 1.0 0.93 0.89 0.95 0.96 0.89 0.95 0.96 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.08 0.18 0.09 0.19 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.18 0.18 0.14 0.11 0.24 0.09 0.16 0 0 0 0.07 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 1 0 0 0 0 1
Bud 0 0 0 0 1 1 2 3 3 2 2 6 0 1 1 0 0 0 0 1 0 0 0 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 2 6 9 2 3 9 5 7 0 2 0 7 10 7 0 0 0 0 0 0
Cytoplasm 287 86 50 227 335 230 289 297 184 193 211 216 218 370 414 189 136 179 284 83 95 176 306 365
Endoplasmic Reticulum 1 1 1 0 1 0 1 7 9 8 1 9 0 0 0 7 4 7 0 1 0 0 0 0
Endosome 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 1
Golgi 1 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0
Mitochondria 7 1 1 2 7 11 24 32 46 27 51 32 0 0 0 1 2 2 0 2 0 0 0 0
Nucleus 0 0 0 1 0 0 6 10 6 7 5 8 1 1 1 4 4 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 1 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 1
Vac/Vac Membrane 3 0 0 9 19 49 67 56 27 69 24 49 2 1 1 14 11 6 0 0 0 1 1 0
Unique Cell Count 296 88 51 233 353 278 365 388 253 285 270 303 220 371 416 204 152 189 296 94 101 185 317 389
Labelled Cell Count 301 89 52 239 366 299 398 409 280 318 300 331 221 375 417 225 169 203 296 94 101 185 317 389


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 6.8 6.0 7.2 8.0 8.5 8.0 8.5 7.4 8.2 7.4 8.4 8.3 7.8 7.9 11.1 12.6 12.7 7.4 7.5 7.5
Std Deviation (1e-4) 3.1 1.3 0.9 1.4 1.3 2.1 1.7 1.8 1.7 1.8 1.7 1.8 1.3 1.1 1.3 2.0 2.4 2.3 2.4 1.2 1.7
Intensity Change (Log2) – – – 0.27 0.42 0.51 0.43 0.51 0.31 0.47 0.31 0.49 0.48 0.39 0.41 0.9 1.09 1.09 0.32 0.33 0.33


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -1.0 -2.8 -3.2 -3.5 -3.9 -4.5 -3.3 -4.1 0.6 1.6 1.2 -1.4 -1.9 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.9 0 3.0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.2 3.3 2.9 0 3.9 0 3.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6176 3.7845 3.2683 2.745 2.1594 3.144 3.0119 4.2847 4.274 3.6857 2.3314 3.9778 1.9285 3.625 2.9482 2.9158 2.5612 3.0709
Actin 0.0226 0.0039 0.0079 0.0071 0.003 0.0067 0.0196 0.0006 0.0071 0.0003 0.0152 0.0008 0.0259 0.0058 0.0089 0.0003 0.003 0.0007
Bud 0.0084 0.0117 0.0126 0.0056 0.0329 0.0055 0.0006 0.0011 0.0011 0.0001 0.0038 0.0001 0.002 0.005 0.003 0.0002 0.001 0.0001
Bud Neck 0.0021 0.0013 0.0011 0.0013 0.0006 0.0072 0.002 0.0001 0.0003 0.0003 0.0022 0.0009 0.0035 0.0004 0.0005 0.0003 0.0008 0.0009
Bud Periphery 0.0043 0.0029 0.0067 0.0022 0.0356 0.0029 0.0006 0.001 0.0025 0 0.007 0 0.0013 0.0013 0.0012 0.0001 0.0023 0.0001
Bud Site 0.0061 0.017 0.0104 0.0021 0.0009 0.001 0.0071 0.0009 0.0034 0.0001 0.0045 0.0001 0.005 0.0095 0.0038 0.0001 0.0031 0.0001
Cell Periphery 0.0004 0.0002 0.0003 0.0002 0.0002 0.0001 0.0003 0 0.0003 0 0.0003 0 0.0003 0.0004 0.0001 0 0.0001 0
Cytoplasm 0.7587 0.854 0.7299 0.8304 0.7452 0.8289 0.7451 0.9386 0.8976 0.9828 0.8603 0.9456 0.8536 0.9293 0.895 0.936 0.9005 0.9497
Cytoplasmic Foci 0.0178 0.0089 0.0163 0.018 0.0248 0.0193 0.0364 0.0096 0.01 0.0018 0.015 0.0116 0.0222 0.0044 0.0103 0.0139 0.0039 0.0043
Eisosomes 0.0005 0 0.0001 0 0 0.0001 0.0003 0 0.0001 0 0.0001 0 0.0004 0.0009 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0054 0.0015 0.004 0.0055 0.0011 0.0023 0.0033 0.0011 0.0017 0.0008 0.0024 0.0015 0.0031 0.0016 0.0021 0.0025 0.0029 0.0019
Endosome 0.0084 0.0023 0.0126 0.0063 0.003 0.0063 0.0227 0.0018 0.0102 0.0004 0.0281 0.0078 0.014 0.0016 0.005 0.0233 0.0129 0.0037
Golgi 0.002 0.0003 0.0016 0.0006 0.0011 0.0006 0.0065 0.0003 0.0046 0 0.0196 0.0032 0.0048 0.0002 0.0029 0.0007 0.0014 0.0004
Lipid Particles 0.0018 0.0001 0.0007 0.0005 0.0003 0.0015 0.0111 0.0001 0.0039 0 0.0029 0.0028 0.0059 0.0002 0.0053 0.0002 0.003 0.0006
Mitochondria 0.0029 0.0005 0.0028 0.0006 0.0005 0.001 0.0131 0.0002 0.0072 0.0001 0.0129 0.0003 0.0035 0.0021 0.0099 0.0002 0.0042 0.0007
None 0.1169 0.0694 0.1439 0.058 0.1154 0.0692 0.0919 0.0398 0.0296 0.0081 0.0081 0.0079 0.034 0.0304 0.0306 0.0119 0.0307 0.0212
Nuclear Periphery 0.0074 0.0017 0.0074 0.0063 0.001 0.0026 0.0035 0.0005 0.0008 0.0006 0.0016 0.0013 0.0023 0.001 0.0039 0.0019 0.0086 0.0039
Nucleolus 0.0055 0.0002 0.0009 0.0005 0.0001 0.0006 0.002 0 0.0001 0 0.0003 0 0.0003 0.0002 0.0003 0 0.0008 0.0004
Nucleus 0.0162 0.017 0.0272 0.0219 0.0071 0.0226 0.0054 0.0026 0.0031 0.004 0.0042 0.0102 0.0046 0.004 0.005 0.0061 0.0109 0.0085
Peroxisomes 0.0026 0.0012 0.0009 0.0007 0.0246 0.0021 0.0154 0.0003 0.0129 0 0.0041 0.0037 0.0096 0.0002 0.0094 0.0002 0.0015 0.0001
Punctate Nuclear 0.0065 0.0046 0.0102 0.0296 0.0018 0.0181 0.0098 0.0007 0.0004 0.0003 0.0009 0.0015 0.0013 0.0008 0.0016 0.0007 0.0017 0.0012
Vacuole 0.0026 0.0012 0.002 0.0022 0.0007 0.0011 0.0026 0.0006 0.0023 0.0002 0.0051 0.0006 0.0018 0.0007 0.0008 0.001 0.0043 0.0009
Vacuole Periphery 0.0007 0.0002 0.0007 0.0005 0.0001 0.0003 0.0006 0.0001 0.001 0 0.0014 0.0001 0.0005 0.0001 0.0003 0.0003 0.0021 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.8969 84.9463 53.3933 47.5568 46.3826 65.9218 64.6694 44.6705 49.1272 47.5481
Translational Efficiency 1.804 1.7303 1.4688 1.6238 1.6402 1.382 1.6026 1.6976 1.5114 1.2213

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Coproporphyrinogen III oxidase; oxygen-requiring enzyme that catalyzes sixth step in heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p); human homolog CPOX can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hem13

Hem13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hem13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available