ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 4 | 0 | 3 | – | 1 | 1 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 1 | 2 | 2 | 2 | – | 0 | 3 | 11 | 8 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 4 | 4 | 7 | 10 | – | 3 | 6 | 3 | 14 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 10 | 9 | 13 | 10 | – | 12 | 10 | 9 | 18 | 3 | 21 | 20 | 36 | 33 | 28 | 0 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 95 | 78 | 27 | 175 | 330 | 335 | 529 | – | 252 | 352 | 253 | 323 | 53 | 125 | 172 | 252 | 279 | 242 | 101 | 87 | 59 | 21 | 158 | 170 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 0 | 1 | 1 | – | 3 | 0 | 2 | 4 | 0 | 0 | 0 | 21 | 5 | 15 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7 | 0 | 0 | 0 | 1 | 11 | 29 | – | 38 | 58 | 98 | 63 | 0 | 0 | 0 | 3 | 4 | 4 | 0 | 1 | 6 | 0 | 0 | 3 |
Nucleus | 1 | 0 | 1 | 0 | 0 | 2 | 2 | – | 2 | 3 | 1 | 4 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 4 | 5 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 2 | 1 | – | 6 | 4 | 0 | 2 | 0 | 0 | 0 | 2 | 8 | 9 | 0 | 12 | 1 | 0 | 0 | 0 |
Unique Cell Count | 100 | 79 | 28 | 175 | 334 | 347 | 550 | 281 | 401 | 319 | 387 | 53 | 125 | 172 | 265 | 291 | 258 | 105 | 109 | 79 | 23 | 166 | 184 | |
Labelled Cell Count | 105 | 80 | 28 | 190 | 351 | 373 | 589 | 321 | 443 | 386 | 448 | 56 | 148 | 193 | 318 | 333 | 303 | 105 | 109 | 79 | 23 | 166 | 184 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 5.7 | 5.4 | 6.0 | 6.2 | 5.1 | 5.3 | – | 4.6 | 4.7 | 4.1 | 4.3 | 5.7 | 6.2 | 6.2 | 8.1 | 7.8 | 7.4 | – | 5.7 | 6.1 |
Std Deviation (1e-4) | 0.5 | 0.8 | 0.6 | 1.3 | 1.4 | 1.2 | 1.8 | – | 1.0 | 1.2 | 1.0 | 1.2 | 0.8 | 0.8 | 0.9 | 1.4 | 1.6 | 1.5 | – | 1.0 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.15 | 0.21 | -0.07 | -0.01 | – | -0.22 | -0.2 | -0.4 | -0.32 | 0.08 | 0.2 | 0.2 | 0.58 | 0.53 | 0.46 | – | 0.09 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.5 | 1.0 | 0 | -0.1 | – | -1.2 | -1.4 | -2.2 | -1.8 | 1.4 | 2.1 | 2.5 | -0.3 | -0.1 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6298 | 0.6526 | 0.577 | 0.82 | 0.545 | 0.8966 | 0.19 | 0.8714 | 0.8144 | 0.7773 | 0.4222 | 0.7209 | -1.3171 | -0.6452 | -1.0203 | -1.1574 | -1.4514 | -0.9365 |
Actin | 0.0596 | 0.0046 | 0.0113 | 0.036 | 0.0516 | 0.0144 | 0.0456 | 0.0025 | 0.0045 | 0.0008 | 0.0062 | 0.017 | 0.0083 | 0.0006 | 0.0155 | 0.021 | 0.0355 | 0.0061 |
Bud | 0.0008 | 0.0006 | 0.0023 | 0.0002 | 0.0021 | 0.0003 | 0.0002 | 0.0008 | 0.0057 | 0.0008 | 0.0319 | 0.0001 | 0.0003 | 0.0001 | 0.0013 | 0.0006 | 0.0005 | 0 |
Bud Neck | 0.0039 | 0.0012 | 0.0005 | 0.0006 | 0.0004 | 0.0024 | 0.0016 | 0.0004 | 0.004 | 0.0005 | 0.0012 | 0.0011 | 0.0007 | 0.0002 | 0.0007 | 0.0034 | 0.0044 | 0.0056 |
Bud Periphery | 0.0023 | 0.0008 | 0.0023 | 0.0002 | 0.0038 | 0.0005 | 0.0003 | 0.0009 | 0.0033 | 0.0007 | 0.0334 | 0.0002 | 0.0005 | 0.0001 | 0.003 | 0.0009 | 0.0009 | 0.0001 |
Bud Site | 0.0043 | 0.0242 | 0.0132 | 0.0006 | 0.0011 | 0.0009 | 0.0043 | 0.018 | 0.0097 | 0.0018 | 0.0024 | 0.0001 | 0.006 | 0.0019 | 0.0014 | 0.0026 | 0.0029 | 0.0002 |
Cell Periphery | 0.0021 | 0.0019 | 0.0011 | 0.0002 | 0.0005 | 0.0004 | 0.0018 | 0.0018 | 0.002 | 0.0004 | 0.0013 | 0.0003 | 0.001 | 0.0005 | 0.0004 | 0.0006 | 0.0004 | 0.0001 |
Cytoplasm | 0.3343 | 0.5052 | 0.3726 | 0.459 | 0.3608 | 0.6538 | 0.4084 | 0.5731 | 0.5334 | 0.3754 | 0.4386 | 0.6584 | 0.5346 | 0.5826 | 0.532 | 0.4821 | 0.4705 | 0.6247 |
Cytoplasmic Foci | 0.0338 | 0.0071 | 0.0113 | 0.0131 | 0.0178 | 0.016 | 0.0257 | 0.0091 | 0.0088 | 0.0095 | 0.012 | 0.0047 | 0.0125 | 0.0064 | 0.0086 | 0.0097 | 0.0115 | 0.0078 |
Eisosomes | 0.001 | 0.0006 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0009 | 0.0001 |
Endoplasmic Reticulum | 0.0099 | 0.0028 | 0.0044 | 0.0018 | 0.002 | 0.0022 | 0.0035 | 0.0034 | 0.0041 | 0.0023 | 0.0028 | 0.0025 | 0.0048 | 0.0029 | 0.0045 | 0.0032 | 0.0021 | 0.0018 |
Endosome | 0.0259 | 0.0031 | 0.0131 | 0.0025 | 0.0117 | 0.0031 | 0.01 | 0.0011 | 0.0078 | 0.002 | 0.0217 | 0.0031 | 0.0076 | 0.0034 | 0.0033 | 0.0068 | 0.0097 | 0.0025 |
Golgi | 0.0092 | 0.0014 | 0.0042 | 0.0019 | 0.0129 | 0.003 | 0.0064 | 0.0003 | 0.0047 | 0.0018 | 0.0346 | 0.0028 | 0.0032 | 0.0004 | 0.0014 | 0.0102 | 0.0086 | 0.0005 |
Lipid Particles | 0.022 | 0.0062 | 0.0074 | 0.0034 | 0.0435 | 0.0014 | 0.0068 | 0.0004 | 0.0016 | 0.0291 | 0.0036 | 0.0019 | 0.0039 | 0.0003 | 0.0019 | 0.0039 | 0.0351 | 0.0005 |
Mitochondria | 0.0188 | 0.001 | 0.0078 | 0.0006 | 0.0356 | 0.0191 | 0.0014 | 0.0002 | 0.0041 | 0.0064 | 0.009 | 0.0006 | 0.0042 | 0.0001 | 0.0006 | 0.0038 | 0.0119 | 0.0001 |
None | 0.405 | 0.4338 | 0.5341 | 0.4611 | 0.4399 | 0.2787 | 0.4485 | 0.3828 | 0.3927 | 0.526 | 0.3892 | 0.3042 | 0.4035 | 0.3973 | 0.4161 | 0.4459 | 0.3816 | 0.3482 |
Nuclear Periphery | 0.0182 | 0.001 | 0.002 | 0.0003 | 0.0004 | 0.0002 | 0.008 | 0.0004 | 0.0012 | 0.0005 | 0.0016 | 0.0006 | 0.0011 | 0.0005 | 0.0009 | 0.0007 | 0.0016 | 0.0003 |
Nucleolus | 0.0041 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0007 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0 |
Nucleus | 0.0062 | 0.0006 | 0.0007 | 0.0003 | 0.0004 | 0.0004 | 0.0007 | 0.0007 | 0.0041 | 0.0012 | 0.002 | 0.0003 | 0.0009 | 0.0008 | 0.001 | 0.0007 | 0.0013 | 0.0003 |
Peroxisomes | 0.0209 | 0.0011 | 0.0048 | 0.0152 | 0.0122 | 0.0015 | 0.0193 | 0.0018 | 0.0007 | 0.0361 | 0.0018 | 0.0006 | 0.0029 | 0 | 0.0032 | 0.0009 | 0.0146 | 0.0002 |
Punctate Nuclear | 0.0089 | 0.001 | 0.0013 | 0.0003 | 0.0008 | 0.0004 | 0.0048 | 0.0004 | 0.0004 | 0.0028 | 0.002 | 0.0007 | 0.0016 | 0.0004 | 0.002 | 0.0006 | 0.0023 | 0.0004 |
Vacuole | 0.006 | 0.0014 | 0.0033 | 0.0023 | 0.0015 | 0.0011 | 0.0014 | 0.0015 | 0.005 | 0.0009 | 0.0034 | 0.0005 | 0.0016 | 0.0012 | 0.0017 | 0.0017 | 0.0018 | 0.0004 |
Vacuole Periphery | 0.0029 | 0.0002 | 0.0017 | 0.0002 | 0.0005 | 0.0002 | 0.0007 | 0.0001 | 0.0008 | 0.0002 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0009 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2.7991 | 2.0018 | 4.4562 | 5.9599 | 2.1775 | 1.119 | 5.313 | 3.4152 | 4.9915 | 3.4525 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Abundance | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |