ORF
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 – 0 0 0 0.05 0.06 0.17 0.12 0.14 0.11 0.11 0 0 0 0 0 0
Cytoplasm 0.95 0.99 0.96 1.0 0.99 0.97 0.96 – 0.9 0.88 0.79 0.83 1.0 1.0 1.0 0.95 0.96 0.94 0.97 0.8 0.75 0.95 0.96 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0 0.05 – 0.14 0.14 0.31 0.16 0 0 0 0 0 0 0 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 4 0 3 – 1 1 1 4 0 1 0 0 0 2 0 0 1 0 0 1
Bud 0 0 0 1 2 2 2 – 0 3 11 8 0 0 0 0 0 1 0 0 1 0 0 0
Bud Neck 0 0 0 4 4 7 10 – 3 6 3 14 0 1 1 0 1 1 0 1 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 10 9 13 10 – 12 10 9 18 3 21 20 36 33 28 0 0 2 0 0 0
Cytoplasm 95 78 27 175 330 335 529 – 252 352 253 323 53 125 172 252 279 242 101 87 59 21 158 170
Endoplasmic Reticulum 1 1 0 0 0 1 1 – 3 0 2 4 0 0 0 21 5 15 0 0 1 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 1 1 0 1 0 0 1 0
Golgi 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 7 0 0 0 1 11 29 – 38 58 98 63 0 0 0 3 4 4 0 1 6 0 0 3
Nucleus 1 0 1 0 0 2 2 – 2 3 1 4 0 0 0 3 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 4 5 4 6 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 2 1 – 6 4 0 2 0 0 0 2 8 9 0 12 1 0 0 0
Unique Cell Count 100 79 28 175 334 347 550 281 401 319 387 53 125 172 265 291 258 105 109 79 23 166 184
Labelled Cell Count 105 80 28 190 351 373 589 321 443 386 448 56 148 193 318 333 303 105 109 79 23 166 184


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.7 5.4 6.0 6.2 5.1 5.3 – 4.6 4.7 4.1 4.3 5.7 6.2 6.2 8.1 7.8 7.4 – 5.7 6.1
Std Deviation (1e-4) 0.5 0.8 0.6 1.3 1.4 1.2 1.8 – 1.0 1.2 1.0 1.2 0.8 0.8 0.9 1.4 1.6 1.5 – 1.0 1.1
Intensity Change (Log2) – – – 0.15 0.21 -0.07 -0.01 – -0.22 -0.2 -0.4 -0.32 0.08 0.2 0.2 0.58 0.53 0.46 – 0.09 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.5 1.0 0 -0.1 – -1.2 -1.4 -2.2 -1.8 1.4 2.1 2.5 -0.3 -0.1 -0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0