ORF
Human Ortholog
Description Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 – 0 0 0 0.05 0.06 0.17 0.12 0.14 0.11 0.11 0 0 0 0 0 0
Cytoplasm 0.95 0.99 0.96 1.0 0.99 0.97 0.96 – 0.9 0.88 0.79 0.83 1.0 1.0 1.0 0.95 0.96 0.94 0.97 0.8 0.75 0.95 0.96 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0 0.05 – 0.14 0.14 0.31 0.16 0 0 0 0 0 0 0 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 4 0 3 – 1 1 1 4 0 1 0 0 0 2 0 0 1 0 0 1
Bud 0 0 0 1 2 2 2 – 0 3 11 8 0 0 0 0 0 1 0 0 1 0 0 0
Bud Neck 0 0 0 4 4 7 10 – 3 6 3 14 0 1 1 0 1 1 0 1 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 10 9 13 10 – 12 10 9 18 3 21 20 36 33 28 0 0 2 0 0 0
Cytoplasm 95 78 27 175 330 335 529 – 252 352 253 323 53 125 172 252 279 242 101 87 59 21 158 170
Endoplasmic Reticulum 1 1 0 0 0 1 1 – 3 0 2 4 0 0 0 21 5 15 0 0 1 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 1 1 0 1 0 0 1 0
Golgi 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 7 0 0 0 1 11 29 – 38 58 98 63 0 0 0 3 4 4 0 1 6 0 0 3
Nucleus 1 0 1 0 0 2 2 – 2 3 1 4 0 0 0 3 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 – 4 5 4 6 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 2 1 – 6 4 0 2 0 0 0 2 8 9 0 12 1 0 0 0
Unique Cell Count 100 79 28 175 334 347 550 281 401 319 387 53 125 172 265 291 258 105 109 79 23 166 184
Labelled Cell Count 105 80 28 190 351 373 589 321 443 386 448 56 148 193 318 333 303 105 109 79 23 166 184


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.7 5.4 6.0 6.2 5.1 5.3 – 4.6 4.7 4.1 4.3 5.7 6.2 6.2 8.1 7.8 7.4 – 5.7 6.1
Std Deviation (1e-4) 0.5 0.8 0.6 1.3 1.4 1.2 1.8 – 1.0 1.2 1.0 1.2 0.8 0.8 0.9 1.4 1.6 1.5 – 1.0 1.1
Intensity Change (Log2) – – – 0.15 0.21 -0.07 -0.01 – -0.22 -0.2 -0.4 -0.32 0.08 0.2 0.2 0.58 0.53 0.46 – 0.09 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.5 1.0 0 -0.1 – -1.2 -1.4 -2.2 -1.8 1.4 2.1 2.5 -0.3 -0.1 -0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6298 0.6526 0.577 0.82 0.545 0.8966 0.19 0.8714 0.8144 0.7773 0.4222 0.7209 -1.3171 -0.6452 -1.0203 -1.1574 -1.4514 -0.9365
Actin 0.0596 0.0046 0.0113 0.036 0.0516 0.0144 0.0456 0.0025 0.0045 0.0008 0.0062 0.017 0.0083 0.0006 0.0155 0.021 0.0355 0.0061
Bud 0.0008 0.0006 0.0023 0.0002 0.0021 0.0003 0.0002 0.0008 0.0057 0.0008 0.0319 0.0001 0.0003 0.0001 0.0013 0.0006 0.0005 0
Bud Neck 0.0039 0.0012 0.0005 0.0006 0.0004 0.0024 0.0016 0.0004 0.004 0.0005 0.0012 0.0011 0.0007 0.0002 0.0007 0.0034 0.0044 0.0056
Bud Periphery 0.0023 0.0008 0.0023 0.0002 0.0038 0.0005 0.0003 0.0009 0.0033 0.0007 0.0334 0.0002 0.0005 0.0001 0.003 0.0009 0.0009 0.0001
Bud Site 0.0043 0.0242 0.0132 0.0006 0.0011 0.0009 0.0043 0.018 0.0097 0.0018 0.0024 0.0001 0.006 0.0019 0.0014 0.0026 0.0029 0.0002
Cell Periphery 0.0021 0.0019 0.0011 0.0002 0.0005 0.0004 0.0018 0.0018 0.002 0.0004 0.0013 0.0003 0.001 0.0005 0.0004 0.0006 0.0004 0.0001
Cytoplasm 0.3343 0.5052 0.3726 0.459 0.3608 0.6538 0.4084 0.5731 0.5334 0.3754 0.4386 0.6584 0.5346 0.5826 0.532 0.4821 0.4705 0.6247
Cytoplasmic Foci 0.0338 0.0071 0.0113 0.0131 0.0178 0.016 0.0257 0.0091 0.0088 0.0095 0.012 0.0047 0.0125 0.0064 0.0086 0.0097 0.0115 0.0078
Eisosomes 0.001 0.0006 0.0004 0.0001 0.0003 0.0002 0.0004 0.0002 0.0008 0.0004 0.0001 0.0001 0.0003 0.0001 0.0003 0.0002 0.0009 0.0001
Endoplasmic Reticulum 0.0099 0.0028 0.0044 0.0018 0.002 0.0022 0.0035 0.0034 0.0041 0.0023 0.0028 0.0025 0.0048 0.0029 0.0045 0.0032 0.0021 0.0018
Endosome 0.0259 0.0031 0.0131 0.0025 0.0117 0.0031 0.01 0.0011 0.0078 0.002 0.0217 0.0031 0.0076 0.0034 0.0033 0.0068 0.0097 0.0025
Golgi 0.0092 0.0014 0.0042 0.0019 0.0129 0.003 0.0064 0.0003 0.0047 0.0018 0.0346 0.0028 0.0032 0.0004 0.0014 0.0102 0.0086 0.0005
Lipid Particles 0.022 0.0062 0.0074 0.0034 0.0435 0.0014 0.0068 0.0004 0.0016 0.0291 0.0036 0.0019 0.0039 0.0003 0.0019 0.0039 0.0351 0.0005
Mitochondria 0.0188 0.001 0.0078 0.0006 0.0356 0.0191 0.0014 0.0002 0.0041 0.0064 0.009 0.0006 0.0042 0.0001 0.0006 0.0038 0.0119 0.0001
None 0.405 0.4338 0.5341 0.4611 0.4399 0.2787 0.4485 0.3828 0.3927 0.526 0.3892 0.3042 0.4035 0.3973 0.4161 0.4459 0.3816 0.3482
Nuclear Periphery 0.0182 0.001 0.002 0.0003 0.0004 0.0002 0.008 0.0004 0.0012 0.0005 0.0016 0.0006 0.0011 0.0005 0.0009 0.0007 0.0016 0.0003
Nucleolus 0.0041 0.0002 0.0002 0 0.0003 0.0001 0.0002 0.0002 0.0005 0.0007 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0009 0
Nucleus 0.0062 0.0006 0.0007 0.0003 0.0004 0.0004 0.0007 0.0007 0.0041 0.0012 0.002 0.0003 0.0009 0.0008 0.001 0.0007 0.0013 0.0003
Peroxisomes 0.0209 0.0011 0.0048 0.0152 0.0122 0.0015 0.0193 0.0018 0.0007 0.0361 0.0018 0.0006 0.0029 0 0.0032 0.0009 0.0146 0.0002
Punctate Nuclear 0.0089 0.001 0.0013 0.0003 0.0008 0.0004 0.0048 0.0004 0.0004 0.0028 0.002 0.0007 0.0016 0.0004 0.002 0.0006 0.0023 0.0004
Vacuole 0.006 0.0014 0.0033 0.0023 0.0015 0.0011 0.0014 0.0015 0.005 0.0009 0.0034 0.0005 0.0016 0.0012 0.0017 0.0017 0.0018 0.0004
Vacuole Periphery 0.0029 0.0002 0.0017 0.0002 0.0005 0.0002 0.0007 0.0001 0.0008 0.0002 0.0009 0.0001 0.0002 0.0001 0.0002 0.0003 0.0009 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2.7991 2.0018 4.4562 5.9599 2.1775 1.119 5.313 3.4152 4.9915 3.4525
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –