Standard name
Human Ortholog
Description Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.09 0 0.05 0 0.09 0.08 0.06 0 0.05 0 0 0 0 0.14 0 0.1 0 0 0.05 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.11 0.07 0.1 0.09 0.11 0.08 0.09 0.11 0.08 0.13 0 0.17 0.08 0.33 0.23 0.28 0 0 0 0 0 0
Cytoplasm 0.52 0.59 0.43 0.48 0.46 0.33 0.35 0.37 0.48 0.39 0.47 0.45 0.46 0.65 0.73 0.42 0.51 0.48 0.15 0.25 0 0.09 0.16 0.22
Endoplasmic Reticulum 0.44 0.4 0.52 0.45 0.43 0.58 0.47 0.4 0.3 0.35 0.3 0.31 0.46 0.27 0.23 0 0 0.05 0.69 0.55 0.85 0.59 0.61 0.47
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.12 0.23 0.17 0 0 0 0 0 0
Mitochondria 0.06 0 0 0.1 0.05 0.09 0.19 0.23 0.18 0.22 0.22 0.17 0 0 0 0.06 0 0 0 0 0 0.17 0.09 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0.11 0.07 0 0.05 0.06 0.07 0.06 0.07 0.07 0 0 0.09 0.05 0.07 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 10 9 2 8 10 22 25 16 11 12 13 2 2 2 9 1 9 1 7 5 8 6 11
Bud 2 2 1 2 1 4 13 12 12 17 12 12 1 1 0 2 0 1 4 12 2 2 2 7
Bud Neck 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 6 1 0 2 3
Bud Site 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 1 9 11 8 16 23 28 24 24 28 19 39 2 27 19 22 9 24 0 1 0 6 4 9
Cytoplasm 53 182 43 53 78 83 89 111 123 101 119 130 28 106 175 28 20 41 18 82 3 16 30 57
Endoplasmic Reticulum 45 125 51 49 73 146 121 121 76 90 76 90 28 44 56 0 1 4 86 185 98 114 114 124
Endosome 0 0 2 0 0 1 1 1 2 1 3 1 0 0 0 0 0 0 1 3 0 0 0 3
Golgi 0 1 1 2 10 0 0 0 0 0 0 1 0 0 2 8 9 15 0 1 0 1 0 0
Mitochondria 6 3 2 11 9 23 49 68 45 57 55 48 0 0 0 4 0 1 5 11 1 32 16 26
Nucleus 0 1 0 0 1 0 0 0 2 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 2 0 2 3 5 3 3 6 6 0 0 1 0 0 0 1 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Vac/Vac Membrane 4 11 4 7 19 17 8 15 15 17 15 19 4 1 3 6 2 6 1 18 1 5 8 15
Unique Cell Count 102 311 99 110 168 251 256 301 256 257 251 290 61 162 239 66 39 86 124 336 116 193 188 264
Labelled Cell Count 115 346 124 136 215 309 335 382 319 326 318 359 65 181 260 79 42 101 124 336 116 193 188 264


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 6.8 8.3 8.6 10.8 10.2 9.5 9.4 9.4 8.7 8.6 8.4 7.8 6.9 6.5 28.6 26.9 26.8 7.3 7.3 7.1
Std Deviation (1e-4) 2.1 2.0 3.8 3.4 4.3 3.9 4.2 3.7 4.1 3.7 4.0 3.4 2.3 1.6 1.5 15.3 16.5 15.0 1.9 1.6 1.4
Intensity Change (Log2) 0.06 0.38 0.3 0.2 0.17 0.19 0.06 0.04 0.02 -0.09 -0.26 -0.34 1.78 1.7 1.69 -0.18 -0.18 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.4 -1.4 -1.9 -0.1 -0.2 -0.9 -1.8 -1.5 -1.7 0 0 0 0.9 0 0.3
Bud 0 0 0 0 0 0 2.2 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -0.4 -0.6 0 -1.0 -0.5 -0.1 -1.1 0.6 0 1.2 -0.9 3.5 1.8 2.9
Cytoplasm 0.7 0.5 -1.8 -1.5 -1.2 0.8 -0.7 0.7 0.2 0.3 3.5 5.2 -0.1 0.8 0.6
Endoplasmic Reticulum -1.0 -1.3 1.1 -0.7 -2.0 -3.8 -2.9 -3.7 -3.7 -0.7 -4.0 -5.1 -7.0 -5.3 -7.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4.0
Mitochondria 2.4 0 2.3 4.1 4.7 3.9 4.6 4.5 3.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.8 2.0 1.0 0 0 0.7 0.9 0.7 0.9 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.397 6.0507 3.4273 2.0699 2.7023 4.4595 4.962 5.958 4.5779 2.6065 3.3816 5.1887 4.5161 5.4762 2.7718 2.9405 1.2139 3.7754
Actin 0.0186 0.0048 0.0258 0.0077 0.0569 0.0071 0.0337 0.0016 0.0056 0.0082 0.0059 0.0118 0.0111 0.0005 0.0197 0.0022 0.0646 0.0143
Bud 0.0002 0.0015 0.0007 0.0016 0.0008 0.0008 0.0006 0.0008 0.0006 0.0021 0.0006 0.0003 0.0001 0 0.0001 0.0002 0.0029 0.0002
Bud Neck 0.0004 0.0006 0.0013 0.0007 0.0025 0.0039 0.0045 0.0005 0.0009 0.0019 0.001 0.0018 0.0002 0.0004 0.0006 0.037 0.0017 0.0056
Bud Periphery 0.0003 0.0078 0.002 0.0056 0.0016 0.0019 0.0017 0.0025 0.0015 0.0154 0.0008 0.0004 0.0001 0.0003 0.0001 0.0008 0.0214 0.0006
Bud Site 0.0015 0.0149 0.0113 0.0016 0.0014 0.0003 0.0052 0.0199 0.0074 0.0063 0.0017 0.0002 0.0004 0.0006 0.0012 0.0026 0.0025 0.0005
Cell Periphery 0.0023 0.0075 0.0055 0.0048 0.0017 0.0026 0.0029 0.01 0.0033 0.005 0.0012 0.0016 0.0014 0.0135 0.0023 0.0087 0.0078 0.0021
Cytoplasm 0.144 0.1697 0.2206 0.381 0.3579 0.1718 0.1528 0.1737 0.2613 0.1915 0.3378 0.1898 0.1593 0.1929 0.2872 0.2196 0.3055 0.2171
Cytoplasmic Foci 0.0128 0.0039 0.014 0.0083 0.0105 0.0052 0.0162 0.0073 0.0191 0.01 0.0215 0.008 0.0069 0.0026 0.0086 0.0058 0.0064 0.0042
Eisosomes 0.0004 0.0002 0.0005 0.0002 0.0018 0.0002 0.0005 0.0002 0.0002 0.0003 0.0001 0.0003 0.0001 0.0001 0.0002 0.0001 0.0007 0.0002
Endoplasmic Reticulum 0.7333 0.7361 0.5908 0.4073 0.3914 0.7558 0.6644 0.7202 0.5607 0.4666 0.3849 0.6944 0.7245 0.7054 0.5088 0.5594 0.2839 0.5968
Endosome 0.0109 0.0046 0.0159 0.0141 0.036 0.0092 0.0185 0.007 0.0199 0.0213 0.0313 0.0133 0.0123 0.0047 0.0112 0.0098 0.0097 0.0065
Golgi 0.0036 0.002 0.0063 0.0023 0.0126 0.0025 0.007 0.0011 0.0061 0.0041 0.0102 0.0047 0.0089 0.0007 0.0062 0.0014 0.0078 0.004
Lipid Particles 0.0063 0.0102 0.0094 0.033 0.0128 0.0039 0.0112 0.0102 0.0163 0.0166 0.0271 0.0067 0.0108 0.0135 0.0101 0.0058 0.0058 0.004
Mitochondria 0.0043 0.0006 0.0053 0.0009 0.0046 0.0004 0.0051 0.0004 0.0028 0.0053 0.0079 0.001 0.002 0.0001 0.0021 0.0001 0.003 0.019
None 0.0257 0.0227 0.063 0.1185 0.072 0.0184 0.0467 0.0261 0.0674 0.2013 0.1236 0.0458 0.0471 0.0446 0.1315 0.1354 0.2472 0.1142
Nuclear Periphery 0.0164 0.0062 0.0109 0.0051 0.0116 0.0069 0.0109 0.0101 0.0073 0.0105 0.0129 0.0054 0.0074 0.0071 0.0036 0.0055 0.0124 0.0031
Nucleolus 0.0004 0.0001 0.0004 0.0001 0.0016 0.0001 0.0004 0.0002 0.0004 0.0004 0.0009 0.0005 0 0 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0043 0.0011 0.0026 0.0017 0.0026 0.0018 0.0014 0.0015 0.0022 0.0042 0.0046 0.0021 0.0008 0.0009 0.0011 0.001 0.0025 0.0009
Peroxisomes 0.0044 0.0001 0.0014 0.0002 0.0051 0.0009 0.0062 0.0001 0.0065 0.0111 0.0021 0.001 0.0034 0 0.0013 0.0001 0.0039 0.0027
Punctate Nuclear 0.0042 0.0008 0.0053 0.0007 0.0106 0.0018 0.0034 0.0004 0.0027 0.0044 0.0139 0.0063 0.0005 0.0003 0.0008 0.0005 0.0008 0.0004
Vacuole 0.0049 0.0041 0.0064 0.004 0.0027 0.0039 0.0058 0.0056 0.0072 0.0121 0.0073 0.0042 0.002 0.0114 0.003 0.0036 0.0084 0.0033
Vacuole Periphery 0.0008 0.0003 0.0008 0.0004 0.0013 0.0006 0.0011 0.0006 0.0006 0.0014 0.0029 0.0004 0.0003 0.0003 0.0002 0.0002 0.001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.7856 11.8217 47.1508 192.7303 45.5438 10.7388 7.4706 9.5801 138.441 101.8135
Translational Efficiency 2.0384 0.6923 1.6224 1.2388 1.7628 0.7248 1.2332 1.7329 1.8839 1.6133

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1678 1857 361 1196 1446 1725 1294 135 3124 3582 1655 1331

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 759.63 891.51 1291.62 1158.59 818.35 835.82 1121.25 1411.24 786.81 864.69 1158.41 1184.22
Standard Deviation 112.75 152.87 246.09 331.65 140.75 124.18 234.13 338.38 129.83 142.53 247.02 340.98
Intensity Change Log 2 0.230954 0.765813 0.609001 0.030474 0.454318 0.786173 0.130465 0.612671 0.703594

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000341 0.002854 0.001260 0.008151 0.001002 0.000961 0.004563 0.018446 0.000647 0.001943 0.003843 0.009195
Bud Neck 0.008865 0.006945 0.002802 0.016947 0.007516 0.008720 0.010239 0.010797 0.008240 0.007800 0.008617 0.016323
Bud Site 0.007669 0.023555 0.015447 0.061117 0.014675 0.014924 0.045357 0.113721* 0.010912 0.019398 0.038833 0.066452
Cell Periphery 0.001132 0.004100 0.007299 0.014951 0.002545 0.002239 0.012388 0.014296 0.001786 0.003204 0.011278 0.014885
Cytoplasm 0.392978* 0.265783* 0.024904 0.266084* 0.389366* 0.280726* 0.198010* 0.144462* 0.391306* 0.272979* 0.160251* 0.253748*
Cytoplasmic Foci 0.135018 0.070841 0.004119 0.051526 0.104511 0.108884 0.019087 0.054685 0.120898 0.089162 0.015822 0.051846
Eisosomes 0.000641 0.001213 0.000153 0.000824 0.000825 0.000825 0.000946 0.000542 0.000726 0.001026 0.000773 0.000795
Endoplasmic Reticulum 0.236810* 0.475801* 0.904503* 0.288380* 0.294029* 0.348687* 0.590200* 0.344775* 0.263295* 0.414586* 0.658758* 0.294100*
Endosome 0.012741 0.013074 0.006840 0.016286 0.010660 0.025915 0.010727 0.028755 0.011778 0.019258 0.009879 0.017551
Golgi 0.009948 0.015070 0.001315 0.004227 0.010399 0.022053 0.005096 0.012282 0.010157 0.018433 0.004271 0.005044
Lipid Particles 0.038551 0.028310 0.006755 0.052496 0.036247 0.057928 0.020668 0.059081 0.037485 0.042573 0.017633 0.053164
Mitochondria 0.006291 0.008861 0.000352 0.002369 0.007342 0.013128 0.004403 0.000396 0.006777 0.010916 0.003519 0.002169
Mitotic Spindle 0.001478 0.001077 0.000248 0.006830 0.001251 0.002882 0.000848 0.004301 0.001373 0.001946 0.000717 0.006574
None 0.007345 0.007034 0.000191 0.009252 0.006319 0.005375 0.006415 0.000113 0.006870 0.006235 0.005057 0.008325
Nuclear Periphery 0.003276 0.001092 0.000595 0.002410 0.002781 0.002903 0.000628 0.000679 0.003047 0.001964 0.000621 0.002234
Nuclear Periphery Foci 0.004290 0.004235 0.006695 0.021219 0.004069 0.005124 0.007114 0.027502 0.004188 0.004663 0.007023 0.021856
Nucleolus 0.000891 0.000377 0.000026 0.000847 0.000789 0.001110 0.000298 0.000061 0.000844 0.000730 0.000239 0.000767
Nucleus 0.074843 0.029335 0.002472 0.056812 0.059712 0.032391 0.022991 0.006627 0.067839 0.030807 0.018515 0.051722
Peroxisomes 0.003665 0.005122 0.000248 0.002352 0.002958 0.005368 0.002007 0.001610 0.003337 0.005241 0.001624 0.002277
Vacuole 0.051340 0.032647 0.012970 0.115755 0.041295 0.054110 0.036531 0.155017 0.046691 0.042983 0.031392 0.119737
Vacuole Periphery 0.001887 0.002672 0.000806 0.001165 0.001710 0.005747 0.001482 0.001853 0.001805 0.004153 0.001335 0.001235

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.53 -3.10 -7.97 -5.05 -6.47 0.19 -6.06 -2.96 -2.97 -2.29 -6.60 -7.09 -8.04 -6.70 -4.48
Bud Neck 1.73 5.08 -4.73 -6.09 -8.43 -1.11 -2.24 -1.88 -1.24 -0.36 0.57 -0.49 -5.59 -5.98 -4.86
Bud Site -7.55 -2.09 -13.26 -8.51 -8.03 -0.12 -8.25 -5.59 -5.58 -3.78 -5.49 -9.73 -13.56 -11.24 -5.48
Cell Periphery -7.01 -3.95 -9.34 -6.86 -3.09 0.50 -8.04 -2.80 -2.89 -0.23 -3.91 -9.80 -9.23 -8.09 -1.52
Cytoplasm 14.17 45.86 11.43 -0.82 -27.77 11.01 18.88 11.35 6.02 1.86 17.81 30.63 14.35 1.20 -10.94
Cytoplasmic Foci 12.85 31.18 16.79 4.83 -15.10 -0.83 20.94 5.40 5.90 -4.02 8.79 33.89 17.73 10.55 -11.53
Eisosomes -3.94 6.79 -1.34 2.89 -6.84 0.00 -0.88 1.91 1.94 2.49 -3.20 -0.47 -0.16 2.70 0.29
Endoplasmic Reticulum -19.49 -47.60* -1.77 15.91 42.47* -4.06 -20.13 -0.62 0.97 7.81 -16.59 -34.93 -0.21 12.72 29.04
Endosome -0.34 4.85 -2.27 -2.06 -6.02 -10.82 -0.17 -4.92 -0.65 -4.87 -8.74 2.35 -4.47 1.78 -5.82
Golgi -4.73 11.48 7.40 11.37 -4.22 -6.97 4.93 -0.21 2.35 -1.42 -8.44 8.31 6.66 13.59 -0.39
Lipid Particles 4.51 14.15 -2.87 -5.65 -11.38 -6.85 5.50 -2.16 -0.05 -3.67 -2.70 9.83 -3.68 -2.21 -9.05
Mitochondria -2.85 8.07 4.38 8.51 -3.33 -3.97 2.96 7.55 11.15 9.29 -4.92 4.94 6.16 11.14 2.42
Mitotic Spindle 0.52 1.93 -4.34 -4.77 -5.63 -1.90 0.64 -1.51 -0.93 -1.63 -1.03 1.33 -4.84 -4.31 -5.44
None 0.27 9.28 -0.48 -0.62 -4.17 0.78 0.02 6.69 6.10 5.49 0.77 1.76 -0.33 -0.68 -1.27
Nuclear Periphery 4.59 5.80 1.25 -3.42 -4.77 -0.22 4.32 4.15 6.67 -0.33 2.90 7.13 1.46 -0.98 -4.80
Nuclear Periphery Foci 0.10 -2.17 -9.99 -9.97 -7.18 -1.64 -3.61 -5.19 -4.97 -4.44 -1.18 -4.30 -11.14 -10.85 -8.70
Nucleolus 3.27 5.31 0.75 -2.71 -5.13 -0.63 2.20 3.16 3.12 7.22 0.82 4.46 0.97 0.06 -3.52
Nucleus 16.87 29.36 3.66 -9.04 -17.76 11.07 13.72 21.38 13.20 7.97 20.03 23.80 3.88 -7.96 -11.30
Peroxisomes -2.97 11.23 3.89 6.48 -8.33 -5.51 2.86 3.59 7.95 1.20 -5.76 6.43 4.21 9.20 -1.95
Vacuole 6.93 12.38 -14.53 -18.31 -20.99 -4.04 1.11 -6.76 -6.04 -6.93 1.78 5.94 -17.06 -17.76 -19.23
Vacuole Periphery -3.74 3.50 4.62 7.65 -0.93 -7.78 0.87 -0.10 5.78 -0.52 -8.59 2.46 4.12 10.76 0.95