Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.97 0.94 0.94 0.91 0.84 – 0.76 0.73 0.79 0.74 1.0 1.0 1.0 0.98 0.99 0.99 0.91 0.91 0.91 0.99 0.91 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0 – 0.17 0.18 0.19 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.07 0.06 0.16 – 0.14 0.13 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 3 1
Bud 0 2 0 0 0 2 1 – 1 1 6 3 0 0 0 0 0 0 5 8 3 0 7 12
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud Site 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 2 0 2 5 4 – 6 9 6 12 0 4 1 8 6 4 0 0 0 0 0 0
Cytoplasm 318 266 177 244 244 286 349 – 205 222 271 310 242 400 387 232 177 183 297 246 174 149 266 278
Endoplasmic Reticulum 3 5 0 0 0 0 1 – 1 3 0 3 1 0 0 12 2 6 0 0 1 0 2 1
Endosome 1 0 0 0 0 0 0 – 0 1 1 3 0 0 0 0 0 0 5 1 0 0 1 0
Golgi 1 0 1 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0
Mitochondria 1 0 0 1 1 23 9 – 46 55 64 94 1 0 0 1 0 1 2 0 1 0 0 0
Nucleus 0 1 2 3 1 0 7 – 8 9 9 14 0 0 0 0 0 2 0 1 3 0 0 0
Nuclear Periphery 0 0 0 0 1 0 0 – 2 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 – 0 0 0 1 0 0 0 0 0 0 2 1 0 0 2 0
Vac/Vac Membrane 3 2 3 14 17 20 67 – 37 39 31 35 0 2 0 3 0 1 2 3 3 0 1 1
Unique Cell Count 325 273 183 259 259 315 415 268 305 341 421 243 401 387 236 178 184 328 270 193 151 292 305
Labelled Cell Count 331 278 185 263 267 336 439 306 343 389 478 244 406 388 256 185 197 328 270 193 151 292 305


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 9.3 9.6 9.6 11.6 7.5 8.7 – 6.9 7.0 6.7 6.6 9.9 9.3 10.6 35.2 34.4 34.5 9.2 9.6 10.4
Std Deviation (1e-4) 2.9 3.0 2.8 2.3 2.7 2.2 2.2 – 1.9 1.7 1.7 1.6 3.0 3.2 3.1 7.8 7.8 9.4 2.7 3.2 3.6
Intensity Change (Log2) – – – -0.0 0.27 -0.35 -0.15 – -0.47 -0.46 -0.52 -0.53 0.05 -0.05 0.15 1.87 1.84 1.84 -0.07 0.0 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 -1.2 -2.5 -4.4 – -5.9 -6.6 -5.3 -6.6 2.3 3.1 3.6 1.1 1.9 1.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.1 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.7 0 – 5.9 6.1 6.3 7.0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.0 2.5 2.4 5.1 – 4.5 4.3 3.3 3.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2229 3.4063 2.6153 1.8747 1.8774 3.1658 3.721 7.4137 5.6846 5.3304 5.3666 6.0065 6.4128 7.0857 6.1715 7.4392 6.8744 7.1967
Actin 0.0046 0.0003 0.0007 0.0006 0.0008 0.0035 0.0186 0.0012 0.0072 0.0004 0.0112 0.0015 0.022 0.0136 0.0504 0.0007 0.0388 0.0134
Bud 0.0005 0.0008 0.0003 0.0002 0.0002 0.0007 0.0003 0.0012 0.0004 0.0004 0.0001 0.0001 0.0005 0.0024 0.002 0.0004 0.0014 0.0007
Bud Neck 0.0003 0.0002 0.0002 0.0002 0.0011 0.0008 0.0005 0.0002 0.0006 0.0003 0.0005 0.0016 0.0012 0.0003 0.0004 0.001 0.0011 0.001
Bud Periphery 0.0003 0.0004 0.0001 0.0001 0.0001 0.0007 0.0002 0.0005 0.0003 0.0001 0.0001 0.0001 0.0004 0.001 0.0019 0.0003 0.001 0.0006
Bud Site 0.0008 0.002 0.0007 0.0001 0.0002 0.0002 0.0011 0.0047 0.001 0.0002 0.0002 0.0001 0.0022 0.0048 0.0036 0.0004 0.0013 0.0001
Cell Periphery 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001
Cytoplasm 0.7563 0.914 0.8537 0.8015 0.7527 0.8909 0.839 0.9482 0.9158 0.9351 0.9038 0.9463 0.6862 0.828 0.69 0.7546 0.6613 0.857
Cytoplasmic Foci 0.0159 0.0044 0.0068 0.0098 0.0079 0.0103 0.0268 0.0047 0.0072 0.0119 0.0083 0.0123 0.014 0.0055 0.0198 0.0132 0.0111 0.0054
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0002 0.0002 0.0005 0 0.0005 0.0001
Endoplasmic Reticulum 0.0065 0.0009 0.003 0.0083 0.0054 0.0017 0.0075 0.001 0.0025 0.0042 0.0046 0.0035 0.0058 0.0026 0.0034 0.0042 0.0025 0.0018
Endosome 0.0081 0.0007 0.0042 0.0063 0.0108 0.0034 0.0058 0.0054 0.0032 0.0327 0.0141 0.0031 0.0178 0.0026 0.0198 0.0708 0.0081 0.0016
Golgi 0.0015 0.0001 0.0002 0.0003 0.0003 0.0003 0.0018 0.0005 0.0013 0.0011 0.0347 0.0005 0.0047 0.0018 0.0062 0.0048 0.0044 0.0006
Lipid Particles 0.0018 0 0.0001 0.0001 0.0003 0.0001 0.0022 0.0001 0.0012 0.0005 0.0009 0.0001 0.0073 0.0006 0.0052 0.002 0.027 0.0003
Mitochondria 0.0038 0.0001 0.0003 0.0003 0.0009 0.0003 0.0004 0.0003 0.0003 0.0003 0.0033 0.0003 0.0017 0.0008 0.0201 0.0035 0.0333 0.0003
None 0.1782 0.0714 0.1195 0.1593 0.1641 0.0781 0.0785 0.0273 0.0501 0.0069 0.0099 0.0181 0.1805 0.1312 0.1445 0.1282 0.1182 0.113
Nuclear Periphery 0.0082 0.0004 0.0025 0.0041 0.0256 0.001 0.0015 0.0005 0.0009 0.0009 0.0014 0.0011 0.0193 0.0007 0.0061 0.0026 0.0038 0.0005
Nucleolus 0.0002 0 0.0001 0 0.0004 0 0.0001 0.0001 0.0001 0 0 0 0.0008 0.0001 0.0006 0.0002 0.0015 0
Nucleus 0.0052 0.003 0.0042 0.0056 0.0214 0.0044 0.003 0.0019 0.0032 0.0022 0.003 0.0048 0.0141 0.0016 0.0038 0.0043 0.0205 0.0024
Peroxisomes 0.0023 0.0001 0.0002 0.0001 0.0002 0.0013 0.0023 0.0001 0.0029 0.0001 0.0004 0.0046 0.0044 0.0008 0.0095 0.0011 0.0276 0.0002
Punctate Nuclear 0.0031 0.0003 0.0013 0.0019 0.0042 0.0015 0.0082 0.0009 0.0008 0.0003 0.0005 0.0009 0.015 0.0005 0.0054 0.0012 0.0351 0.0005
Vacuole 0.0015 0.0007 0.0016 0.001 0.0024 0.0006 0.0016 0.0006 0.0008 0.0018 0.0026 0.0007 0.0011 0.0007 0.0055 0.005 0.0008 0.0003
Vacuole Periphery 0.0006 0.0001 0.0004 0.0003 0.0008 0.0001 0.0002 0.0001 0.0001 0.0003 0.0003 0.0001 0.0007 0.0001 0.0013 0.0013 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.2162 33.0999 29.4738 25.8362 25.6038 27.1381 37.9295 33.1293 33.8004 29.0032
Translational Efficiency 1.7462 1.2669 1.4164 1.4831 1.0553 2.5931 1.4347 2.1263 1.624 1.6173

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1736 400 282 125 1624 2112 199 1979 3360 2512 481 2104

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 832.88 1040.16 1001.47 960.28 797.38 996.00 1012.24 917.87 815.72 1003.03 1005.93 920.39
Standard Deviation 145.34 248.70 131.63 152.06 144.28 177.42 117.32 138.20 145.91 191.24 126.02 139.43
Intensity Change Log 2 — 0.320625 0.265939 0.205346 — 0.320878 0.344212 0.203022 — 0.320749 0.304754 0.204210

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000111 0.000273 0.000463 0.000274 0.000104 0.000274 0.000402 0.000238 0.000108 0.000274 0.000438 0.000240
Bud Neck 0.003798 0.003145 0.002520 0.001399 0.002269 0.004649 0.001810 0.005824 0.003059 0.004410 0.002226 0.005561
Bud Site 0.005674 0.020780 0.006161 0.003902 0.005525 0.010776 0.004077 0.024340 0.005602 0.012369 0.005299 0.023126
Cell Periphery 0.000080 0.000144 0.000206 0.000154 0.000080 0.000068 0.000268 0.000100 0.000080 0.000080 0.000231 0.000103
Cytoplasm 0.740297* 0.770375* 0.397576* 0.337579* 0.770562* 0.785704* 0.308957* 0.638876* 0.754925* 0.783263* 0.360912* 0.620976*
Cytoplasmic Foci 0.102604 0.093112 0.013401 0.034130 0.082785 0.067504 0.022436 0.016903 0.093025 0.071582 0.017139 0.017926
Eisosomes 0.000027 0.000022 0.000053 0.000086 0.000024 0.000021 0.000112 0.000028 0.000026 0.000021 0.000078 0.000032
Endoplasmic Reticulum 0.001335 0.000757 0.005196 0.003700 0.001058 0.000646 0.005461 0.001013 0.001201 0.000664 0.005306 0.001173
Endosome 0.005379 0.005523 0.003579 0.010995 0.004559 0.004684 0.005660 0.002555 0.004983 0.004817 0.004440 0.003057
Golgi 0.001409 0.001014 0.000116 0.051550 0.000731 0.001223 0.000127 0.002052 0.001081 0.001190 0.000121 0.004993
Lipid Particles 0.002084 0.001065 0.002198 0.002257 0.001776 0.001061 0.003053 0.000618 0.001935 0.001062 0.002552 0.000716
Mitochondria 0.001218 0.001004 0.000362 0.000467 0.001545 0.001164 0.000266 0.001096 0.001376 0.001138 0.000322 0.001059
Mitotic Spindle 0.000938 0.004586 0.001999 0.004511 0.001856 0.009138 0.000699 0.026083 0.001382 0.008413 0.001461 0.024802
None 0.007476 0.004565 0.003806 0.001419 0.011166 0.006202 0.004101 0.005536 0.009259 0.005942 0.003928 0.005291
Nuclear Periphery 0.000209 0.000112 0.001765 0.001326 0.000182 0.000186 0.001381 0.001716 0.000196 0.000174 0.001606 0.001692
Nuclear Periphery Foci 0.001115 0.002244 0.003392 0.003544 0.001500 0.000693 0.005240 0.000689 0.001301 0.000940 0.004157 0.000858
Nucleolus 0.000742 0.000637 0.000450 0.000142 0.000676 0.000389 0.000551 0.000322 0.000710 0.000428 0.000492 0.000312
Nucleus 0.068894 0.034080 0.457645* 0.242963* 0.070839 0.054533 0.456094* 0.159168* 0.069834 0.051276 0.457004* 0.164146*
Peroxisomes 0.000970 0.003207 0.000295 0.000215 0.000679 0.001194 0.000364 0.000197 0.000830 0.001515 0.000323 0.000198
Vacuole 0.054338 0.050886 0.098630 0.294171* 0.041251 0.049056 0.178738 0.110400 0.048013 0.049347 0.131773 0.121318
Vacuole Periphery 0.001299 0.002471 0.000189 0.005216 0.000831 0.000836 0.000204 0.002245 0.001073 0.001096 0.000195 0.002421

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.86 -7.63 -4.24 -0.44 3.58 -1.96 -8.29 -3.44 0.61 4.99 -2.33 -10.56 -3.78 0.68 6.57
Bud Neck 1.01 0.71 3.31 1.73 2.96 -4.91 -0.45 -3.18 0.50 -2.96 -2.92 0.25 -1.76 0.37 -1.95
Bud Site -4.13 -0.30 1.10 4.33 0.80 -3.90 0.10 -4.44 -1.18 -4.27 -5.74 -0.23 -4.68 -0.05 -3.75
Cell Periphery -3.11 -4.97 -3.74 0.05 2.37 2.52 -7.66 0.97 -1.64 8.04 0.43 -7.98 0.24 -0.17 7.96
Cytoplasm -1.37 24.71 19.61 18.26 1.07 -1.60 27.23 30.65 33.16 -12.94 -4.03 36.33 34.21 35.63 -14.68
Cytoplasmic Foci 1.32 24.88 17.33 9.55 -2.57 3.89 16.84 22.10 21.29 2.35 6.63 29.95 33.42 23.83 0.90
Eisosomes 1.75 -7.52 -9.08 -9.33 -2.59 1.33 -10.82 -6.27 -6.83 9.53 2.60 -12.82 -8.19 -8.95 10.08
Endoplasmic Reticulum 2.41 -8.97 -3.80 -4.98 3.75 1.65 -8.63 -0.76 -3.43 8.74 2.78 -12.44 -1.02 -4.64 12.48
Endosome 0.31 1.98 0.42 0.17 -0.61 0.09 -0.79 6.15 5.83 7.10 0.74 0.93 9.18 6.32 8.16
Golgi 1.57 5.07 -1.23 -1.30 -1.40 -1.71 7.34 0.01 1.04 -1.70 -0.22 6.99 -0.38 -0.27 -2.13
Lipid Particles 2.56 -1.13 -0.47 -3.53 0.80 1.85 -3.01 3.04 0.89 7.78 2.92 -2.82 4.81 0.74 9.29
Mitochondria 0.52 3.34 3.89 1.04 2.22 0.83 2.91 1.94 1.64 -3.87 0.84 4.17 2.76 1.72 -3.09
Mitotic Spindle -1.76 -1.20 -1.60 -0.80 -0.89 -4.29 -0.68 -7.24 -3.58 -6.29 -5.05 -1.32 -7.82 -3.92 -5.58
None 2.71 4.22 1.95 -0.04 -0.57 3.71 4.26 5.24 1.57 -0.40 3.62 6.17 5.60 1.40 -1.67
Nuclear Periphery 2.48 -12.50 -11.80 -12.63 2.98 -0.13 -14.04 -5.94 -5.91 0.19 0.54 -17.36 -6.25 -6.30 1.26
Nuclear Periphery Foci -4.18 -7.04 -5.87 -2.74 0.66 6.10 -5.06 5.96 -0.92 6.08 3.28 -8.33 3.85 0.06 9.22
Nucleolus 0.76 1.85 2.85 1.47 2.90 2.52 1.99 3.62 1.35 2.06 2.50 2.57 3.76 1.79 2.59
Nucleus 9.08 -21.67 -7.02 -8.84 10.12 5.32 -14.85 -26.93 -31.46 8.56 7.89 -26.00 -29.44 -33.38 15.55
Peroxisomes -1.67 4.58 5.63 2.37 1.66 -2.59 3.71 5.78 6.21 4.86 -2.46 5.78 8.04 5.64 4.03
Vacuole -1.27 -8.56 -14.23 -13.53 -9.03 -3.68 -11.73 -29.55 -27.50 2.70 -2.07 -14.22 -31.16 -29.41 -1.56
Vacuole Periphery -0.78 4.35 0.17 0.73 -1.06 0.11 5.19 -0.04 -0.11 -2.43 0.17 6.13 0.70 0.46 -2.56
Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ubc5

Ubc5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubc5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available