TheCellVision

UBC5

Standard name	UBC5 ORF YDR059C Aliases E2 ubiquitin-conjugating protein UBC5
Human Ortholog	UBE2D1
Description	Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication

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Scale images

UBC5

Micrographs

Sub-cellular Localization

LOC-score
Cell count

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Cytoplasm	0.98	0.97	0.97	0.94	0.94	0.91	0.84	–	0.76	0.73	0.79	0.74	1.0	1.0	1.0	0.98	0.99	0.99	0.91	0.91	0.91	0.99	0.91	0.91
Endoplasmic Reticulum	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0.05	0	0	0	0	0	0	0	0
Endosome	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	0	0	0	0.07	0	–	0.17	0.18	0.19	0.22	0	0	0	0	0	0	0	0	0	0	0	0
Nucleus	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
Vac/Vac Membrane	0	0	0	0.05	0.07	0.06	0.16	–	0.14	0.13	0.09	0.08	0	0	0	0	0	0	0	0	0	0	0	0

	ensLOC																		DeepLoc
Localization	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	WT1	WT2	WT3	AF100	AF140	AF180
Cortical Patches	0	1	0	0	0	0	1	–	0	0	0	0	0	0	0	0	0	0	1	1	0	0	3	1
Bud	0	2	0	0	0	2	1	–	1	1	6	3	0	0	0	0	0	0	5	8	3	0	7	12
Bud Neck	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	1	3
Bud Site	0	1	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	–	–	–	–	–	–
Cell Periphery	4	0	2	0	2	5	4	–	6	9	6	12	0	4	1	8	6	4	0	0	0	0	0	0
Cytoplasm	318	266	177	244	244	286	349	–	205	222	271	310	242	400	387	232	177	183	297	246	174	149	266	278
Endoplasmic Reticulum	3	5	0	0	0	0	1	–	1	3	0	3	1	0	0	12	2	6	0	0	1	0	2	1
Endosome	1	0	0	0	0	0	0	–	0	1	1	3	0	0	0	0	0	0	5	1	0	0	1	0
Golgi	1	0	1	0	1	0	0	–	0	0	0	0	0	0	0	0	0	0	4	0	0	0	0	0
Mitochondria	1	0	0	1	1	23	9	–	46	55	64	94	1	0	0	1	0	1	2	0	1	0	0	0
Nucleus	0	1	2	3	1	0	7	–	8	9	9	14	0	0	0	0	0	2	0	1	3	0	0	0
Nuclear Periphery	0	0	0	0	1	0	0	–	2	2	1	2	0	0	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	0	0	0	–	0	2	0	1	0	0	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	1	0	0	0	–	0	0	0	1	0	0	0	0	0	0	2	1	0	0	2	0
Vac/Vac Membrane	3	2	3	14	17	20	67	–	37	39	31	35	0	2	0	3	0	1	2	3	3	0	1	1
Unique Cell Count	325	273	183	259	259	315	415		268	305	341	421	243	401	387	236	178	184	328	270	193	151	292	305
Labelled Cell Count	331	278	185	263	267	336	439		306	343	389	478	244	406	388	256	185	197	328	270	193	151	292	305

Yeast GFP Assignment

Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)

Protein Abundance

Screen	WT1	WT2	WT3	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3	AF100	AF140	AF180
Mean Cell GFP Intensity (1e^-4)	9.8	9.3	9.6	9.6	11.6	7.5	8.7	–	6.9	7.0	6.7	6.6	9.9	9.3	10.6	35.2	34.4	34.5	9.2	9.6	10.4
Std Deviation (1e^-4)	2.9	3.0	2.8	2.3	2.7	2.2	2.2	–	1.9	1.7	1.7	1.6	3.0	3.2	3.1	7.8	7.8	9.4	2.7	3.2	3.6
Intensity Change (Log₂)	–	–	–	-0.0	0.27	-0.35	-0.15	–	-0.47	-0.46	-0.52	-0.53	0.05	-0.05	0.15	1.87	1.84	1.84	-0.07	0.0	0.12

Localization Change

Localization	RAP60	RAP140	RAP220	RAP300	RAP380	RAP460	RAP540	RAP620	RAP700	HU80	HU120	HU160	rpd3Δ_1	rpd3Δ_2	rpd3Δ_3
Cortical Patches	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Bud	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Bud Neck	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Bud Site	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Cell Periphery	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Cytoplasm	-1.2	-1.2	-2.5	-4.4	–	-5.9	-6.6	-5.3	-6.6	2.3	3.1	3.6	1.1	1.9	1.9
Endoplasmic Reticulum	0	0	0	0	–	0	0	0	0	0	0	0	3.1	0	0
Endosome	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Golgi	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Mitochondria	0	0	3.7	0	–	5.9	6.1	6.3	7.0	0	0	0	0	0	0
Nucleus	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Nuclear Periphery	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Nucleolus	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Peroxisomes	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
SpindlePole	0	0	0	0	–	0	0	0	0	0	0	0	0	0	0
Vacuole	2.0	2.5	2.4	5.1	–	4.5	4.3	3.3	3.1	0	0	0	0	0	0

External localization resources

dHITS | Loqate | YeastGFP | YeastRGB | YeastRC

Images

Protein Concentration and Protein Localization Data

	R1						R2						R3
	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Pre-START	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Concentration	2.2229	3.4063	2.6153	1.8747	1.8774	3.1658	3.721	7.4137	5.6846	5.3304	5.3666	6.0065	6.4128	7.0857	6.1715	7.4392	6.8744	7.1967
Actin	0.0046	0.0003	0.0007	0.0006	0.0008	0.0035	0.0186	0.0012	0.0072	0.0004	0.0112	0.0015	0.022	0.0136	0.0504	0.0007	0.0388	0.0134
Bud	0.0005	0.0008	0.0003	0.0002	0.0002	0.0007	0.0003	0.0012	0.0004	0.0004	0.0001	0.0001	0.0005	0.0024	0.002	0.0004	0.0014	0.0007
Bud Neck	0.0003	0.0002	0.0002	0.0002	0.0011	0.0008	0.0005	0.0002	0.0006	0.0003	0.0005	0.0016	0.0012	0.0003	0.0004	0.001	0.0011	0.001
Bud Periphery	0.0003	0.0004	0.0001	0.0001	0.0001	0.0007	0.0002	0.0005	0.0003	0.0001	0.0001	0.0001	0.0004	0.001	0.0019	0.0003	0.001	0.0006
Bud Site	0.0008	0.002	0.0007	0.0001	0.0002	0.0002	0.0011	0.0047	0.001	0.0002	0.0002	0.0001	0.0022	0.0048	0.0036	0.0004	0.0013	0.0001
Cell Periphery	0.0001	0.0001	0.0001	0	0	0	0.0002	0.0001	0.0001	0	0.0001	0	0.0001	0.0002	0.0002	0.0001	0.0001	0.0001
Cytoplasm	0.7563	0.914	0.8537	0.8015	0.7527	0.8909	0.839	0.9482	0.9158	0.9351	0.9038	0.9463	0.6862	0.828	0.69	0.7546	0.6613	0.857
Cytoplasmic Foci	0.0159	0.0044	0.0068	0.0098	0.0079	0.0103	0.0268	0.0047	0.0072	0.0119	0.0083	0.0123	0.014	0.0055	0.0198	0.0132	0.0111	0.0054
Eisosomes	0	0	0	0	0	0	0.0001	0	0	0	0	0	0.0002	0.0002	0.0005	0	0.0005	0.0001
Endoplasmic Reticulum	0.0065	0.0009	0.003	0.0083	0.0054	0.0017	0.0075	0.001	0.0025	0.0042	0.0046	0.0035	0.0058	0.0026	0.0034	0.0042	0.0025	0.0018
Endosome	0.0081	0.0007	0.0042	0.0063	0.0108	0.0034	0.0058	0.0054	0.0032	0.0327	0.0141	0.0031	0.0178	0.0026	0.0198	0.0708	0.0081	0.0016
Golgi	0.0015	0.0001	0.0002	0.0003	0.0003	0.0003	0.0018	0.0005	0.0013	0.0011	0.0347	0.0005	0.0047	0.0018	0.0062	0.0048	0.0044	0.0006
Lipid Particles	0.0018	0	0.0001	0.0001	0.0003	0.0001	0.0022	0.0001	0.0012	0.0005	0.0009	0.0001	0.0073	0.0006	0.0052	0.002	0.027	0.0003
Mitochondria	0.0038	0.0001	0.0003	0.0003	0.0009	0.0003	0.0004	0.0003	0.0003	0.0003	0.0033	0.0003	0.0017	0.0008	0.0201	0.0035	0.0333	0.0003
None	0.1782	0.0714	0.1195	0.1593	0.1641	0.0781	0.0785	0.0273	0.0501	0.0069	0.0099	0.0181	0.1805	0.1312	0.1445	0.1282	0.1182	0.113
Nuclear Periphery	0.0082	0.0004	0.0025	0.0041	0.0256	0.001	0.0015	0.0005	0.0009	0.0009	0.0014	0.0011	0.0193	0.0007	0.0061	0.0026	0.0038	0.0005
Nucleolus	0.0002	0	0.0001	0	0.0004	0	0.0001	0.0001	0.0001	0	0	0	0.0008	0.0001	0.0006	0.0002	0.0015	0
Nucleus	0.0052	0.003	0.0042	0.0056	0.0214	0.0044	0.003	0.0019	0.0032	0.0022	0.003	0.0048	0.0141	0.0016	0.0038	0.0043	0.0205	0.0024
Peroxisomes	0.0023	0.0001	0.0002	0.0001	0.0002	0.0013	0.0023	0.0001	0.0029	0.0001	0.0004	0.0046	0.0044	0.0008	0.0095	0.0011	0.0276	0.0002
Punctate Nuclear	0.0031	0.0003	0.0013	0.0019	0.0042	0.0015	0.0082	0.0009	0.0008	0.0003	0.0005	0.0009	0.015	0.0005	0.0054	0.0012	0.0351	0.0005
Vacuole	0.0015	0.0007	0.0016	0.001	0.0024	0.0006	0.0016	0.0006	0.0008	0.0018	0.0026	0.0007	0.0011	0.0007	0.0055	0.005	0.0008	0.0003
Vacuole Periphery	0.0006	0.0001	0.0004	0.0003	0.0008	0.0001	0.0002	0.0001	0.0001	0.0003	0.0003	0.0001	0.0007	0.0001	0.0013	0.0013	0.0005	0.0001

Sequencing Data

	R1					R2
	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase	G1 Post-START	S/G2	Metaphase	Anaphase	Telophase
Gene Expression	22.2162	33.0999	29.4738	25.8362	25.6038	27.1381	37.9295	33.1293	33.8004	29.0032
Translational Efficiency	1.7462	1.2669	1.4164	1.4831	1.0553	2.5931	1.4347	2.1263	1.624	1.6173

Hit Data

Dataset	Hit
Protein Concentration	✘
Protein Localization	✘
Gene Expression	✔
Translational Efficiency	✘

Proteome Dynamics

Micrographs

Cell Count

R1				R2				R1 & R2
WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
1736	400	282	125	1624	2112	199	1979	3360	2512	481	2104

Protein Abundance

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Mean	832.88	1040.16	1001.47	960.28	797.38	996.00	1012.24	917.87	815.72	1003.03	1005.93	920.39
Standard Deviation	145.34	248.70	131.63	152.06	144.28	177.42	117.32	138.20	145.91	191.24	126.02	139.43
Intensity Change Log ₂	—	0.320625	0.265939	0.205346	—	0.320878	0.344212	0.203022	—	0.320749	0.304754	0.204210

Localization Score (DeepLoc)

	R1				R2				R1 & R2
	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14	WT	UBP2	UBP14	UBP2UBP14
Actin	0.000111	0.000273	0.000463	0.000274	0.000104	0.000274	0.000402	0.000238	0.000108	0.000274	0.000438	0.000240
Bud Neck	0.003798	0.003145	0.002520	0.001399	0.002269	0.004649	0.001810	0.005824	0.003059	0.004410	0.002226	0.005561
Bud Site	0.005674	0.020780	0.006161	0.003902	0.005525	0.010776	0.004077	0.024340	0.005602	0.012369	0.005299	0.023126
Cell Periphery	0.000080	0.000144	0.000206	0.000154	0.000080	0.000068	0.000268	0.000100	0.000080	0.000080	0.000231	0.000103
Cytoplasm	0.740297*	0.770375*	0.397576*	0.337579*	0.770562*	0.785704*	0.308957*	0.638876*	0.754925*	0.783263*	0.360912*	0.620976*
Cytoplasmic Foci	0.102604	0.093112	0.013401	0.034130	0.082785	0.067504	0.022436	0.016903	0.093025	0.071582	0.017139	0.017926
Eisosomes	0.000027	0.000022	0.000053	0.000086	0.000024	0.000021	0.000112	0.000028	0.000026	0.000021	0.000078	0.000032
Endoplasmic Reticulum	0.001335	0.000757	0.005196	0.003700	0.001058	0.000646	0.005461	0.001013	0.001201	0.000664	0.005306	0.001173
Endosome	0.005379	0.005523	0.003579	0.010995	0.004559	0.004684	0.005660	0.002555	0.004983	0.004817	0.004440	0.003057
Golgi	0.001409	0.001014	0.000116	0.051550	0.000731	0.001223	0.000127	0.002052	0.001081	0.001190	0.000121	0.004993
Lipid Particles	0.002084	0.001065	0.002198	0.002257	0.001776	0.001061	0.003053	0.000618	0.001935	0.001062	0.002552	0.000716
Mitochondria	0.001218	0.001004	0.000362	0.000467	0.001545	0.001164	0.000266	0.001096	0.001376	0.001138	0.000322	0.001059
Mitotic Spindle	0.000938	0.004586	0.001999	0.004511	0.001856	0.009138	0.000699	0.026083	0.001382	0.008413	0.001461	0.024802
None	0.007476	0.004565	0.003806	0.001419	0.011166	0.006202	0.004101	0.005536	0.009259	0.005942	0.003928	0.005291
Nuclear Periphery	0.000209	0.000112	0.001765	0.001326	0.000182	0.000186	0.001381	0.001716	0.000196	0.000174	0.001606	0.001692
Nuclear Periphery Foci	0.001115	0.002244	0.003392	0.003544	0.001500	0.000693	0.005240	0.000689	0.001301	0.000940	0.004157	0.000858
Nucleolus	0.000742	0.000637	0.000450	0.000142	0.000676	0.000389	0.000551	0.000322	0.000710	0.000428	0.000492	0.000312
Nucleus	0.068894	0.034080	0.457645*	0.242963*	0.070839	0.054533	0.456094*	0.159168*	0.069834	0.051276	0.457004*	0.164146*
Peroxisomes	0.000970	0.003207	0.000295	0.000215	0.000679	0.001194	0.000364	0.000197	0.000830	0.001515	0.000323	0.000198
Vacuole	0.054338	0.050886	0.098630	0.294171*	0.041251	0.049056	0.178738	0.110400	0.048013	0.049347	0.131773	0.121318
Vacuole Periphery	0.001299	0.002471	0.000189	0.005216	0.000831	0.000836	0.000204	0.002245	0.001073	0.001096	0.000195	0.002421

Localization Changes (T-score)

	R1					R2					R1 & R2
	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14	UBP2_WT	UBP14_WT	UBP2UBP14_WT	UBP2UBP14_UBP2	UBP2UBP14_UBP14
Actin	-5.86	-7.63	-4.24	-0.44	3.58	-1.96	-8.29	-3.44	0.61	4.99	-2.33	-10.56	-3.78	0.68	6.57
Bud Neck	1.01	0.71	3.31	1.73	2.96	-4.91	-0.45	-3.18	0.50	-2.96	-2.92	0.25	-1.76	0.37	-1.95
Bud Site	-4.13	-0.30	1.10	4.33	0.80	-3.90	0.10	-4.44	-1.18	-4.27	-5.74	-0.23	-4.68	-0.05	-3.75
Cell Periphery	-3.11	-4.97	-3.74	0.05	2.37	2.52	-7.66	0.97	-1.64	8.04	0.43	-7.98	0.24	-0.17	7.96
Cytoplasm	-1.37	24.71	19.61	18.26	1.07	-1.60	27.23	30.65	33.16	-12.94	-4.03	36.33	34.21	35.63	-14.68
Cytoplasmic Foci	1.32	24.88	17.33	9.55	-2.57	3.89	16.84	22.10	21.29	2.35	6.63	29.95	33.42	23.83	0.90
Eisosomes	1.75	-7.52	-9.08	-9.33	-2.59	1.33	-10.82	-6.27	-6.83	9.53	2.60	-12.82	-8.19	-8.95	10.08
Endoplasmic Reticulum	2.41	-8.97	-3.80	-4.98	3.75	1.65	-8.63	-0.76	-3.43	8.74	2.78	-12.44	-1.02	-4.64	12.48
Endosome	0.31	1.98	0.42	0.17	-0.61	0.09	-0.79	6.15	5.83	7.10	0.74	0.93	9.18	6.32	8.16
Golgi	1.57	5.07	-1.23	-1.30	-1.40	-1.71	7.34	0.01	1.04	-1.70	-0.22	6.99	-0.38	-0.27	-2.13
Lipid Particles	2.56	-1.13	-0.47	-3.53	0.80	1.85	-3.01	3.04	0.89	7.78	2.92	-2.82	4.81	0.74	9.29
Mitochondria	0.52	3.34	3.89	1.04	2.22	0.83	2.91	1.94	1.64	-3.87	0.84	4.17	2.76	1.72	-3.09
Mitotic Spindle	-1.76	-1.20	-1.60	-0.80	-0.89	-4.29	-0.68	-7.24	-3.58	-6.29	-5.05	-1.32	-7.82	-3.92	-5.58
None	2.71	4.22	1.95	-0.04	-0.57	3.71	4.26	5.24	1.57	-0.40	3.62	6.17	5.60	1.40	-1.67
Nuclear Periphery	2.48	-12.50	-11.80	-12.63	2.98	-0.13	-14.04	-5.94	-5.91	0.19	0.54	-17.36	-6.25	-6.30	1.26
Nuclear Periphery Foci	-4.18	-7.04	-5.87	-2.74	0.66	6.10	-5.06	5.96	-0.92	6.08	3.28	-8.33	3.85	0.06	9.22
Nucleolus	0.76	1.85	2.85	1.47	2.90	2.52	1.99	3.62	1.35	2.06	2.50	2.57	3.76	1.79	2.59
Nucleus	9.08	-21.67	-7.02	-8.84	10.12	5.32	-14.85	-26.93	-31.46	8.56	7.89	-26.00	-29.44	-33.38	15.55
Peroxisomes	-1.67	4.58	5.63	2.37	1.66	-2.59	3.71	5.78	6.21	4.86	-2.46	5.78	8.04	5.64	4.03
Vacuole	-1.27	-8.56	-14.23	-13.53	-9.03	-3.68	-11.73	-29.55	-27.50	2.70	-2.07	-14.22	-31.16	-29.41	-1.56
Vacuole Periphery	-0.78	4.35	0.17	0.73	-1.06	0.11	5.19	-0.04	-0.11	-2.43	0.17	6.13	0.70	0.46	-2.56