ORF
Human Ortholog
Description Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.09 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0.06 0
Golgi 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0.05 0.06 0.13 0.44 0.59 0.51 0 0.08 0.05 0.08 0.09 0.07
Mitochondria 0.9 0.89 0.94 0.92 0.86 0.91 0.88 0.86 0.96 0.94 0.95 0.89 0.92 0.87 0.85 0.06 0.09 0.11 0.83 0.75 0.8 0.74 0.65 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0.06 0.05 0 0.05 0.11 0.11 0.12 0.08 0.1 0.12 0.07 0.05 0 0 0.06 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0.08 0.08 0.09 0.08 0.08 0.12 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0.08 0.06 0.09 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.13 0.07 0 0.08 0.06 0.06 0 0 0 0.05 0 0.09 0.1 0.09 0.15 0.24 0.24 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 4 1 0 0 1 0 1 0 0 1 0 1 1 1 1 4 1 3 3 4 5 9
Bud 1 2 0 12 5 7 12 14 10 20 5 3 3 1 1 0 2 0 1 2 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Bud Site 0 1 0 0 0 2 5 7 0 2 1 3 0 0 0 1 0 0
Cell Periphery 1 14 12 5 4 0 2 3 0 4 0 3 4 6 7 8 5 4 0 1 0 0 0 0
Cytoplasm 6 3 0 6 6 3 9 8 3 3 2 4 5 8 7 14 10 3 0 1 0 3 0 0
Endoplasmic Reticulum 2 3 3 4 0 3 4 2 1 1 1 2 0 2 2 3 3 0 0 0 1 0 0 0
Endosome 1 3 5 5 20 7 9 18 0 1 1 3 3 3 4 6 5 1 5 7 10 3 12 8
Golgi 0 13 24 6 5 0 2 5 2 0 0 1 7 12 29 43 64 19 2 20 15 11 18 14
Mitochondria 126 220 285 232 257 382 475 427 316 362 177 167 138 165 192 6 10 4 110 194 242 107 129 143
Nucleus 2 1 1 1 0 4 5 5 2 1 1 2 1 0 1 3 2 1 0 0 0 0 0 0
Nuclear Periphery 10 14 16 9 16 45 61 58 25 37 23 14 7 5 5 6 1 0 0 1 1 1 2 1
Nucleolus 12 4 5 6 10 32 44 42 25 29 23 13 2 1 4 4 4 1 0 0 0 1 2 2
Peroxisomes 0 6 7 6 13 6 13 7 0 2 2 3 2 4 10 5 7 0 4 11 24 8 17 15
SpindlePole 1 1 0 1 5 3 16 12 0 4 0 2 0 1 0 3 4 0 0 0 0 0 2 0
Vac/Vac Membrane 15 32 20 2 25 26 33 21 9 11 10 7 13 18 20 15 26 9 2 9 2 1 3 9
Unique Cell Count 140 248 302 252 298 419 540 494 328 384 187 187 150 189 227 97 109 37 133 258 304 146 199 220
Labelled Cell Count 177 317 382 296 366 520 691 629 394 478 246 228 185 227 283 118 144 46 133 258 304 146 199 220


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 8.0 7.4 6.7 7.0 6.3 5.7 6.2 6.1 5.4 5.6 5.8 7.4 7.7 7.9 11.0 12.7 13.9 7.9 7.8 8.7
Std Deviation (1e-4) 1.4 1.4 1.3 1.3 1.4 1.1 1.3 1.3 1.2 1.3 1.1 1.7 1.5 1.4 1.4 3.1 2.9 3.1 2.4 2.0 1.7
Intensity Change (Log2) -0.13 -0.08 -0.24 -0.37 -0.24 -0.28 -0.44 -0.39 -0.35 0.0 0.06 0.1 0.57 0.78 0.91 0.1 0.08 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 3.8 0 0 0 3.0 0 4.0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -2.0 -4.1 -3.9 -3.4 -3.6 -2.5 0 -1.5 -0.7 -0.5 -0.5 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 3.1 0 0 1.6 0 0 0 0 0 0 0 0 0 0
Golgi -2.9 -3.6 -5.9 -6.1 -5.1 -4.6 -5.6 -4.0 -3.6 -1.3 -0.7 1.8 8.3 11.1 0
Mitochondria -1.1 -3.4 -1.6 -3.0 -3.5 1.2 -0.1 0.1 -2.1 -1.0 -2.8 -3.7 -17.0 -16.9 -13.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.0 0 2.6 2.9 3.0 1.2 2.1 2.8 1.0 -0.3 -1.4 -1.8 0.3 0 0
Nucleolus 0.6 1.3 3.6 3.9 4.0 3.5 3.5 4.9 3.0 0 0 0 0 0 0
Peroxisomes 0 1.4 -0.9 0.1 -0.9 0 0 0 0 0 0 1.3 0 0 0
SpindlePole 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.5 0.8 -0.2 -0.3 -1.5 -2.3 -2.4 -0.6 -1.4 0.8 1.2 0.9 2.7 4.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0542 0.0276 0.0221 0.0008 0.0011 0.007 0.0562 0.0095 0.0237 0.0016 0.0046 0.0032 0.1132 0.0766 0.0464 0.0007 0.0034 0.0254
Bud 0.0004 0.0016 0.0061 0.0002 0.0002 0.0007 0.001 0.0005 0.0011 0.0002 0.0043 0.0002 0.0003 0.0005 0.0009 0.0001 0.0003 0.0001
Bud Neck 0.0004 0.0004 0.0033 0.0001 0.0002 0.0009 0.0011 0.0003 0.0014 0 0.0003 0.0002 0.0004 0.0006 0.0009 0.0006 0.0001 0.0005
Bud Periphery 0.0009 0.0018 0.0229 0.0005 0.0005 0.003 0.0018 0.0011 0.0014 0.0011 0.0273 0.0004 0.0004 0.0007 0.0031 0.0001 0.0012 0.0003
Bud Site 0.0013 0.0195 0.0117 0.0002 0.0004 0.0004 0.0056 0.003 0.0344 0.0002 0.0009 0.0002 0.0008 0.0017 0.0033 0.0001 0.0002 0.0002
Cell Periphery 0.0003 0.0003 0.0122 0.0001 0.0002 0.0005 0.0008 0.0004 0.0027 0.0003 0.0019 0.0001 0.0002 0.0001 0.0007 0.0002 0.0002 0.0002
Cytoplasm 0.0021 0.001 0.0013 0 0.0001 0.0003 0.0057 0.0003 0.0045 0.0011 0.0005 0.0018 0 0.0006 0.0007 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0054 0.0138 0.0034 0.001 0.0034 0.0031 0.0604 0.0239 0.0281 0.0027 0.0146 0.0163 0.0017 0.0093 0.0061 0.0272 0.0007 0.0038
Eisosomes 0.0012 0.0005 0.0014 0.0001 0.0001 0.0003 0.0008 0.0005 0.001 0.0003 0.0003 0.0002 0.0017 0.0005 0.0015 0.0001 0.0003 0.0003
Endoplasmic Reticulum 0.0035 0.0007 0.0006 0.0001 0.0002 0.0008 0.0457 0.0002 0.0003 0.0007 0.0001 0.001 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001
Endosome 0.0168 0.024 0.0049 0.0089 0.0231 0.0202 0.1005 0.0088 0.0263 0.0154 0.0084 0.018 0.0036 0.0086 0.0082 0.0066 0.0119 0.0057
Golgi 0.0167 0.0207 0.0101 0.008 0.022 0.0172 0.0596 0.0147 0.0191 0.0037 0.0161 0.032 0.027 0.0393 0.0308 0.0153 0.0168 0.0184
Lipid Particles 0.0169 0.0384 0.011 0.0013 0.0302 0.0096 0.0567 0.0533 0.0136 0.0017 0.0625 0.0311 0.0033 0.0015 0.001 0.0508 0.0022 0.0086
Mitochondria 0.8109 0.798 0.8642 0.9724 0.8947 0.9047 0.5136 0.8559 0.7794 0.959 0.7754 0.8441 0.8396 0.8331 0.8782 0.798 0.9544 0.9203
None 0.0068 0.0002 0.0003 0 0.0001 0.0002 0.0045 0.0001 0.0007 0.0018 0.0004 0.0112 0.0001 0.0001 0.0011 0 0.0001 0.0001
Nuclear Periphery 0.0184 0.0002 0.0006 0.0001 0.0001 0.0017 0.0304 0.0001 0.0001 0.0002 0.0001 0.0003 0 0 0.0001 0 0.0002 0.0001
Nucleolus 0.0008 0.0008 0.0004 0 0.0003 0.0001 0.0006 0.0004 0.0003 0.0001 0.0004 0.0001 0 0 0.0001 0.0002 0.0001 0.0001
Nucleus 0.0045 0.0002 0.0003 0.0001 0.0002 0.0002 0.0028 0.0001 0.0002 0.0002 0.0002 0.0001 0 0 0.0001 0.0001 0.0001 0.0001
Peroxisomes 0.025 0.0453 0.0164 0.0025 0.0154 0.022 0.0349 0.0248 0.0529 0.0056 0.0777 0.0369 0.0065 0.0258 0.0143 0.0968 0.0011 0.013
Punctate Nuclear 0.0062 0.0001 0.0001 0 0.0001 0.0001 0.0095 0.0006 0.0002 0 0.0007 0.0003 0 0.0001 0 0.0002 0 0.0001
Vacuole 0.0029 0.0026 0.0032 0.0012 0.0032 0.0026 0.0039 0.0008 0.0037 0.0023 0.0017 0.0008 0.0002 0.0002 0.0007 0.0011 0.0018 0.0008
Vacuole Periphery 0.0043 0.0024 0.0037 0.0022 0.0042 0.0044 0.004 0.0007 0.0048 0.0018 0.0016 0.0013 0.0006 0.0004 0.0015 0.0018 0.0044 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.9412 18.2662 18.5242 19.2755 12.8049 21.1028 27.0878 28.878 24.5089 23.8271
Translational Efficiency 0.5351 0.8275 0.5444 0.6254 0.8241 0.5074 0.6944 0.5124 0.6439 0.6135

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1281 1484 587 1818 1530 1939 1548 59 2811 3423 2135 1877

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1058.98 1483.90 1832.16 1458.58 1346.81 1494.82 1546.07 1609.09 1215.64 1490.09 1624.73 1463.31
Standard Deviation 156.42 289.65 233.94 233.38 217.96 259.08 260.33 292.62 239.91 272.81 283.73 236.93
Intensity Change Log 2 0.486719 0.790870 0.461889 0.150425 0.199059 0.256699 0.308182 0.489757 0.350633

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000139 0.000379 0.000400 0.001565 0.000202 0.000118 0.000369 0.001391 0.000174 0.000231 0.000377 0.001559
Bud Neck 0.000379 0.000482 0.002081 0.003423 0.000531 0.000412 0.001651 0.003351 0.000462 0.000443 0.001769 0.003421
Bud Site 0.001922 0.002374 0.001775 0.010614 0.003053 0.001171 0.003979 0.008926 0.002538 0.001693 0.003373 0.010561
Cell Periphery 0.000075 0.000074 0.000201 0.000102 0.000060 0.000031 0.000154 0.000216 0.000067 0.000050 0.000167 0.000105
Cytoplasm 0.818962* 0.713393* 0.430066* 0.541956* 0.762880* 0.723950* 0.502569* 0.511334* 0.788437* 0.719373* 0.482635* 0.540994*
Cytoplasmic Foci 0.019452 0.006316 0.004611 0.005158 0.008847 0.004736 0.003441 0.027911 0.013680 0.005421 0.003763 0.005873
Eisosomes 0.000007 0.000003 0.000004 0.000007 0.000004 0.000001 0.000013 0.000025 0.000005 0.000002 0.000010 0.000008
Endoplasmic Reticulum 0.000795 0.000353 0.000534 0.000512 0.000666 0.000141 0.001162 0.000782 0.000725 0.000233 0.000990 0.000520
Endosome 0.000774 0.000594 0.000467 0.005099 0.000972 0.000291 0.001117 0.074714 0.000882 0.000422 0.000939 0.007287
Golgi 0.000632 0.001127 0.000254 0.005424 0.000521 0.000734 0.000541 0.002320 0.000572 0.000905 0.000462 0.005326
Lipid Particles 0.000494 0.000268 0.000779 0.000709 0.000251 0.000093 0.000604 0.005279 0.000362 0.000169 0.000652 0.000853
Mitochondria 0.000385 0.000957 0.000500 0.003364 0.000180 0.000176 0.000982 0.000730 0.000273 0.000515 0.000849 0.003282
Mitotic Spindle 0.001777 0.001869 0.005610 0.061276 0.002100 0.005201 0.006458 0.069789 0.001953 0.003757 0.006225 0.061543
None 0.007718 0.015394 0.012081 0.019154 0.007748 0.015490 0.012641 0.007208 0.007734 0.015448 0.012487 0.018778
Nuclear Periphery 0.000125 0.000113 0.000829 0.001293 0.000166 0.000095 0.000747 0.000433 0.000147 0.000103 0.000770 0.001266
Nuclear Periphery Foci 0.002501 0.002475 0.001773 0.002448 0.003845 0.001024 0.002195 0.004315 0.003232 0.001653 0.002079 0.002506
Nucleolus 0.000214 0.000135 0.000171 0.000287 0.000103 0.000208 0.000175 0.000165 0.000154 0.000176 0.000174 0.000283
Nucleus 0.131363* 0.245039* 0.516863* 0.300054* 0.196367* 0.241047* 0.415400* 0.155525* 0.166744* 0.242777* 0.443296* 0.295511*
Peroxisomes 0.000199 0.000250 0.000042 0.001042 0.000213 0.000137 0.000236 0.006098 0.000206 0.000186 0.000182 0.001201
Vacuole 0.011780 0.007826 0.020873 0.033036 0.011170 0.004826 0.045020 0.119211 0.011448 0.006126 0.038381 0.035745
Vacuole Periphery 0.000304 0.000578 0.000086 0.003477 0.000123 0.000117 0.000547 0.000278 0.000206 0.000317 0.000420 0.003376

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.02 -10.30 -2.87 -0.36 2.51 2.41 -3.12 -3.25 -3.95 -2.02 -2.31 -8.35 -2.63 -1.85 0.02
Bud Neck -5.50 -15.45 -15.30 -13.68 3.27 0.87 -7.83 -5.53 -5.73 -2.08 -1.44 -14.15 -14.97 -14.73 -2.34
Bud Site -1.41 -4.57 -5.01 -3.37 -0.55 2.25 -0.16 -3.95 -6.90 -4.32 1.41 -1.76 -3.71 -5.52 -2.26
Cell Periphery -2.26 -13.10 -6.14 -2.92 11.44 6.76 -5.41 -4.53 -5.37 -2.18 1.69 -11.51 -9.33 -10.61 7.01
Cytoplasm 17.15 49.13 52.72 34.57 -9.14 6.79 47.09 11.05 9.93 1.56 16.59 66.92 54.72 42.85 -6.01
Cytoplasmic Foci 9.34 8.77 13.58 4.09 6.30 4.83 9.08 -0.31 -2.15 -3.37 10.31 14.33 14.03 3.66 -0.71
Eisosomes 3.17 -4.93 -7.88 -11.94 -1.91 5.22 -7.56 -7.66 -8.49 -4.60 5.61 -8.00 -13.90 -20.25 -1.38
Endoplasmic Reticulum 4.88 -1.61 2.29 -4.31 4.15 6.13 -2.90 -1.67 -5.42 0.07 7.95 -3.31 1.60 -10.49 6.17
Endosome 1.30 -1.65 -1.08 -1.99 -0.01 1.65 0.38 -1.05 -1.14 -1.07 2.05 0.07 -1.12 -2.87 -1.39
Golgi -0.74 0.38 -0.26 0.44 -0.67 -0.42 1.10 0.03 0.43 -0.92 -0.82 1.14 -0.48 0.07 -1.10
Lipid Particles 0.54 -6.90 -3.99 -11.70 5.45 5.36 -8.05 -4.85 -5.21 -3.75 1.53 -7.44 -9.03 -18.10 -1.54
Mitochondria -0.91 -1.89 -1.76 -0.38 -0.95 0.03 -2.79 -1.66 -1.56 1.98 -0.85 -2.79 -2.30 -1.52 -0.82
Mitotic Spindle -0.07 -4.38 -8.75 -9.06 -5.06 -1.99 -5.02 -2.39 -1.99 -1.38 -1.79 -6.46 -9.20 -8.33 -5.65
None -6.71 -1.75 -6.47 0.52 -4.55 -11.31 -5.93 0.53 3.90 2.48 -12.44 -5.99 -7.44 1.35 -2.70
Nuclear Periphery -1.55 -19.69 -22.90 -22.82 0.47 5.76 -22.84 -6.88 -7.23 -1.31 3.05 -30.03 -23.17 -23.66 -4.34
Nuclear Periphery Foci -1.07 -2.22 4.46 5.61 6.26 9.51 6.50 0.67 -3.12 -2.12 6.56 4.49 8.75 2.71 4.59
Nucleolus 0.85 -0.80 -1.37 -7.11 -2.49 -1.33 -4.31 -2.70 0.47 1.72 -0.63 -2.31 -3.91 -2.11 -3.39
Nucleus -19.74 -38.43 -33.15 -12.94 16.07 -8.65 -36.35 0.62 2.66 11.43 -19.52 -52.92 -29.39 -15.11 19.28
Peroxisomes -0.10 1.26 0.23 0.32 -0.81 0.46 0.76 -0.88 -0.98 -1.04 0.31 1.25 0.04 -0.20 -0.91
Vacuole 0.27 -12.27 -27.89 -28.16 -8.89 4.63 -16.06 -9.15 -9.33 -7.42 3.82 -20.19 -28.83 -29.87 -12.37
Vacuole Periphery -0.68 1.02 -0.88 -0.09 -1.76 0.25 -1.03 -0.71 -0.89 0.77 -0.55 -0.07 -1.48 -1.06 -1.43