Standard name
Human Ortholog
Description Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.97 0.94 0.9 0.8 0.78 0.72 0.76 1.0 1.0 1.0 0.95 0.98 0.95 0.93 0.92 0.94 0.95 0.96 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.08 0.15 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0.11 0.13 0.14 0.08 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Bud 1 0 0 1 0 1 1 2 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 0 3 2 5 3 14 1 11 0 1 2 4 2 5 0 0 0 0 0 0
Cytoplasm 116 138 250 131 250 221 242 122 221 219 335 369 146 145 115 39 112 31 181 272 281
Endoplasmic Reticulum 0 0 1 1 2 5 6 3 20 0 0 0 1 3 7 0 0 0 1 2 0
Endosome 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 2 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 0 0 1 0 1
Mitochondria 1 1 2 6 4 16 26 26 31 0 0 0 2 2 1 0 3 0 0 0 0
Nucleus 1 2 1 4 1 12 6 3 8 1 0 0 1 4 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 1 2 6 2 29 31 41 24 24 1 0 0 5 3 4 0 0 0 1 2 1
Unique Cell Count 119 141 257 140 278 275 309 169 291 220 335 369 153 148 121 42 122 33 192 285 294
Labelled Cell Count 123 143 264 147 291 291 337 181 317 221 336 371 161 160 135 42 122 33 192 285 294


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 9.2 10.7 7.9 10.1 9.6 9.0 9.0 9.4 11.2 11.2 11.2 12.0 12.7 12.9 9.2 10.0 9.8
Std Deviation (1e-4) 1.7 1.6 1.7 1.9 1.7 1.9 1.7 2.2 2.0 1.8 1.8 1.8 2.9 3.8 3.5 1.9 2.2 2.0
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.3992 11.7552 10.4921 9.6433 9.5609 10.0194 11.3343 15.9582 14.6119 12.6929 12.6346 14.7022 12.8951 16.0757 14.8286 13.8623 13.0597 14.2052
Actin 0.0057 0.0002 0.0062 0.0002 0.0182 0.0003 0.0337 0.0001 0.0062 0.0182 0.0513 0.0001 0.0072 0.0003 0.0003 0.0007 0.0165 0.018
Bud 0.0007 0.0001 0.0001 0.0001 0.0006 0.0001 0.0005 0 0.0001 0.0004 0.0005 0 0.0005 0.0001 0.0001 0.0001 0.0006 0.0001
Bud Neck 0.0008 0.0001 0.0004 0.0004 0.0013 0.0009 0.0018 0.0001 0.0004 0.0008 0.0014 0.0005 0.0005 0.0001 0.0002 0.0004 0.0012 0.0012
Bud Periphery 0.0004 0 0.0001 0 0.0008 0 0.0006 0 0 0.0007 0.0008 0 0.0003 0.0001 0 0 0.0005 0.0001
Bud Site 0.0006 0.0001 0.0001 0 0.0004 0 0.0041 0 0.0004 0.0026 0.0013 0 0.0005 0.0002 0.0001 0.0001 0.0005 0.0004
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0002 0 0 0.001 0.0001 0 0.0001 0.0001 0 0 0.0001 0.0002
Cytoplasm 0.8671 0.9925 0.9784 0.9874 0.9218 0.9736 0.8485 0.996 0.9711 0.9122 0.8832 0.9862 0.9094 0.9833 0.9741 0.9476 0.89 0.9521
Cytoplasmic Foci 0.0104 0.0009 0.0014 0.0012 0.0026 0.0027 0.0218 0.0004 0.0019 0.0072 0.0034 0.0007 0.0116 0.0005 0.0086 0.0096 0.0037 0.0033
Eisosomes 0 0 0 0 0 0 0.0004 0 0 0.001 0.0002 0 0 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0055 0.0006 0.0027 0.0012 0.0086 0.0039 0.0061 0.0002 0.0015 0.006 0.002 0.0006 0.0086 0.0003 0.0003 0.0003 0.0014 0.0023
Endosome 0.0354 0.0005 0.0013 0.0015 0.0175 0.0021 0.0146 0.0001 0.0011 0.0094 0.0188 0.0002 0.016 0.0001 0.0012 0.003 0.0099 0.0046
Golgi 0.0041 0.0001 0.0004 0 0.0035 0.0001 0.0037 0 0.0002 0.0039 0.0054 0 0.0062 0 0.0007 0.0021 0.0277 0.0016
Lipid Particles 0.0014 0 0.0001 0 0.0007 0 0.0054 0 0.0003 0.0211 0.0074 0 0.0119 0 0.0081 0.0122 0.0056 0.0035
Mitochondria 0.0071 0 0.0001 0.0001 0.0024 0.0001 0.0126 0 0.0085 0.0028 0.0009 0 0.0013 0.0121 0.0003 0.0004 0.0261 0.0014
None 0.0067 0.0007 0.0009 0.0006 0.0008 0.0011 0.0088 0.0005 0.0011 0.0016 0.0015 0.0005 0.0043 0.0005 0.001 0.0005 0.0016 0.0009
Nuclear Periphery 0.0177 0.0007 0.0014 0.0013 0.0104 0.0022 0.0105 0.0003 0.0008 0.0013 0.0068 0.0011 0.0054 0.0002 0.0004 0.0003 0.0011 0.0006
Nucleolus 0.0004 0 0 0 0.0001 0 0.0006 0 0 0.0009 0.0003 0 0.0001 0 0 0 0.0001 0.0001
Nucleus 0.0267 0.0033 0.0057 0.005 0.0063 0.0094 0.0135 0.0021 0.0046 0.0043 0.0051 0.0097 0.005 0.0018 0.0034 0.0025 0.0099 0.0061
Peroxisomes 0.001 0 0 0 0.0002 0 0.0045 0 0.001 0.0018 0.0048 0 0.0035 0 0.0009 0.0198 0.0018 0.0022
Punctate Nuclear 0.0036 0.0001 0.0003 0.0002 0.0005 0.0028 0.0055 0.0001 0.0002 0.0009 0.0039 0.0002 0.0062 0.0001 0.0003 0.0003 0.0005 0.0004
Vacuole 0.003 0.0001 0.0004 0.0007 0.0024 0.0003 0.0018 0 0.0002 0.0017 0.0006 0.0001 0.001 0.0001 0.0001 0.0001 0.0006 0.0006
Vacuole Periphery 0.0015 0 0.0001 0.0002 0.0009 0.0001 0.0009 0 0.0001 0.0005 0.0005 0 0.0004 0 0 0.0001 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 108.2332 97.6101 99.116 138.2845 86.9296 74.6683 118.6054 108.0976 102.7869 128.8334
Translational Efficiency 2.2945 2.3759 2.4106 1.3407 2.6279 3.8942 2.1326 1.7881 2.4297 2.2621

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency