Standard name
Description Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in synthesis of the storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.06 0.07 0 0.05 0.05 0.07 0.06 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.94 0.97 0.95 0.94 0.86 0.76 0.75 0.72 0.76 0.66 0.97 0.96 0.97 0.96 0.95 0.88 0.95 0.94 0.92 0.8 0.78 0.84
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.09 0.14 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0.13 0.14 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.13 0.18 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0.1 0.12 0.07 0.15 0 0 0 0 0 0.15 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 1 3 7 7 6 6 4 7 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 1 0 0 1 1 3 0 0 1 1 2 1 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 5 5 1 3 9 14 6 6 7 8 10 1 4 4 4 22 6 0 0 0 0 0 0
Cytoplasm 244 257 106 83 126 148 175 163 90 99 88 103 171 214 253 225 283 304 220 254 114 146 203 219
Endoplasmic Reticulum 3 4 5 1 7 5 18 31 6 6 1 8 3 7 8 6 6 14 4 2 4 23 36 24
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 2 2 2 1 1 0 1 0 1 4 3 0 0 0 0 1 1
Mitochondria 1 2 0 1 0 0 6 2 15 18 21 19 1 1 0 1 0 0 0 1 0 2 4 2
Nucleus 0 1 1 0 0 0 0 3 0 1 1 1 1 0 0 1 2 0 1 2 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 1 1 1 1 2 12 12 16 8 23 3 1 1 8 4 53 1 2 1 7 9 5
Unique Cell Count 246 264 113 86 133 158 204 215 120 138 116 156 177 222 260 235 298 345 233 269 124 184 263 262
Labelled Cell Count 251 271 118 88 139 166 223 227 138 158 133 172 181 229 268 247 321 381 233 269 124 184 263 262


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.6 13.3 14.8 11.7 15.4 15.2 16.6 17.4 17.0 15.6 15.9 18.3 17.6 31.5 37.8 22.9 24.3 22.3 11.7 13.1 15.4
Std Deviation (1e-4) 3.3 3.2 5.5 2.6 3.0 3.1 4.6 5.1 6.1 5.6 5.4 8.1 6.2 13.4 17.3 6.3 5.7 8.1 1.9 2.7 3.2
Intensity Change (Log2) – – – -0.34 0.06 0.04 0.17 0.23 0.2 0.07 0.1 0.31 0.25 1.09 1.35 0.63 0.71 0.59 -0.34 -0.18 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.5 0.9 0 0.2 0.2 0.8 0.7 0 0 0 0 1.1 0
Cytoplasm 0.9 0.3 0 -2.2 -4.0 -3.9 -4.5 -3.8 -5.4 1.1 1.1 1.6 0.8 0.5 -1.7
Endoplasmic Reticulum 0 0.3 0 1.4 2.8 0.2 0 0 0.3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.9 4.0 4.7 3.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 3.0 3.4 0 3.9 0 0 0 0 0 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.8843 20.4326 18.1599 17.8803 13.8025 18.2435 23.6733 27.9892 25.192 23.8693 21.9574 23.9876 28.6639 32.1801 30.6731 29.734 28.3934 29.3144
Actin 0.0123 0.0009 0.0053 0.0005 0.004 0.0013 0.0004 0.0009 0.0007 0.0014 0.0006 0.0004 0.0008 0.0003 0.0005 0.0001 0.0002 0.0003
Bud 0.0011 0.001 0.0012 0.0063 0.0012 0.0006 0.0008 0.0015 0.0026 0.0015 0.0016 0.0014 0.0009 0.0032 0.0012 0.0006 0.0008 0.0008
Bud Neck 0.0031 0.0005 0.0008 0.0013 0.001 0.0014 0.0005 0.0006 0.0008 0.001 0.0009 0.0012 0.0006 0.0006 0.0006 0.0004 0.0006 0.0012
Bud Periphery 0.0012 0.0006 0.0009 0.0071 0.0013 0.0004 0.0007 0.0011 0.0023 0.0012 0.0007 0.0023 0.001 0.0016 0.001 0.0005 0.0004 0.0009
Bud Site 0.0022 0.0027 0.0009 0.001 0.0009 0.0002 0.001 0.0014 0.0019 0.0004 0.0006 0.0002 0.0011 0.0051 0.0009 0.0003 0.0004 0.0001
Cell Periphery 0.0014 0.0008 0.0011 0.0033 0.0005 0.0003 0.0023 0.0012 0.0012 0.0008 0.0004 0.0007 0.0029 0.0021 0.0024 0.001 0.0007 0.0008
Cytoplasm 0.7347 0.9315 0.8446 0.8759 0.7469 0.8948 0.8835 0.927 0.902 0.8921 0.8958 0.901 0.8923 0.9282 0.9163 0.9159 0.9422 0.9137
Cytoplasmic Foci 0.0168 0.0048 0.0103 0.0059 0.0215 0.0092 0.0057 0.0036 0.0053 0.0042 0.0107 0.0076 0.0033 0.0037 0.0032 0.0026 0.004 0.0044
Eisosomes 0.0002 0 0 0 0.0002 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0538 0.0279 0.035 0.0256 0.0207 0.0284 0.0461 0.0287 0.02 0.0218 0.022 0.0384 0.0575 0.0157 0.0287 0.0322 0.0053 0.0466
Endosome 0.055 0.012 0.0302 0.0184 0.0674 0.0307 0.0165 0.0087 0.0182 0.0155 0.0227 0.0186 0.0087 0.0079 0.0077 0.0075 0.0076 0.0115
Golgi 0.008 0.0019 0.004 0.0008 0.0072 0.0031 0.0019 0.0015 0.0012 0.0008 0.0011 0.0025 0.0014 0.0006 0.0007 0.0004 0.0003 0.0017
Lipid Particles 0.0046 0.0002 0.0019 0.0002 0.0147 0.0002 0.0007 0.0002 0.0003 0.0004 0.0001 0.0001 0.0003 0.0003 0.0003 0.0001 0.0002 0.0001
Mitochondria 0.0062 0.0011 0.0098 0.0006 0.027 0.0132 0.0013 0.0011 0.0013 0.0005 0.0007 0.0046 0.0019 0.0006 0.0004 0.0004 0.0002 0.003
None 0.0088 0.0006 0.0011 0.0005 0.029 0.0006 0.0009 0.0005 0.0006 0.0004 0.0006 0.0008 0.0007 0.0007 0.0005 0.0005 0.0005 0.0005
Nuclear Periphery 0.0109 0.0024 0.0093 0.005 0.0128 0.0036 0.0025 0.0017 0.0018 0.0033 0.0023 0.0018 0.0028 0.0013 0.0021 0.0021 0.0011 0.0013
Nucleolus 0.0005 0 0.0002 0.0001 0.001 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0001 0 0
Nucleus 0.0049 0.0013 0.0065 0.0031 0.0097 0.0013 0.0015 0.0018 0.0018 0.0024 0.0021 0.0013 0.0016 0.0022 0.0033 0.0018 0.0016 0.0011
Peroxisomes 0.0075 0 0.0005 0.0001 0.0027 0.0001 0.0001 0 0 0 0.0002 0 0 0 0 0 0 0
Punctate Nuclear 0.0042 0.0001 0.0075 0.0001 0.0017 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0
Vacuole 0.0535 0.0084 0.024 0.0394 0.0222 0.0078 0.03 0.0168 0.0346 0.0471 0.0323 0.0136 0.0204 0.0244 0.0282 0.0304 0.0302 0.0099
Vacuole Periphery 0.0089 0.0013 0.005 0.005 0.0067 0.0027 0.0034 0.0017 0.0034 0.0049 0.0043 0.0033 0.0018 0.0014 0.0018 0.003 0.0036 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.4222 26.3913 28.2873 67.4873 31.1003 45.4887 43.8252 65.2865 85.7199 50.8147
Translational Efficiency 0.9632 0.6697 1.0997 1.0055 0.9426 1.0412 0.8363 0.7842 0.967 0.8577

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘