Standard name
Description Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.09 0.09 0.10 0.16 0.12 0.11 0.10 0.09 0.08 0.10 0.13 0.17 0.23 0.29 0.13 0.17 0.14 0 0.06 0 0.09 0.06 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.19 0.13 0.12 0.08 0.59 0.67 0.66 0.71 0.68 0.75 0.68 0 0 0 0.05 0.07 0 0 0 0 0 0 0
Nucleus 0.82 0.74 0.72 0.78 0.63 0.51 0.48 0.52 0.44 0.43 0.43 0.37 0.76 0.74 0.66 0.68 0.72 0.70 0.82 0.69 0.72 0.53 0.54 0.47
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.10 0.15 0.10 0.19 0.11 0.08 0.07 0.16 0.10 0.09 0.17 0.09 0.06 0.11 0.23 0.11 0.23 0 0.14 0.16 0.25 0.29 0.30
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 4 5 5 7 7 6 1 0 0 0 0 0 0 0 2 3 1 3 5
Bud Neck 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 1 0 0 1 5
Bud Site 0 0 0 0 0 1 8 6 9 14 17 4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 4 27 16 19 44 46 44 55 27 28 41 52 20 45 51 12 22 16 2 19 8 12 18 15
Endoplasmic Reticulum 0 1 0 1 0 0 1 0 1 0 0 1 2 0 1 7 7 6 0 0 1 0 1 1
Endosome 0 3 4 0 17 2 1 0 2 3 1 5 0 1 1 0 2 0 0 5 1 0 4 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 1 1 0 0
Mitochondria 7 56 24 24 23 225 266 351 214 248 306 281 3 0 0 5 9 1 2 10 2 6 5 8
Nucleus 31 220 133 155 172 194 188 280 132 159 176 153 88 144 116 62 94 80 58 234 144 76 174 125
Nuclear Periphery 0 0 0 2 0 1 2 5 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 3 3 3 1 2 0 0 0 0 0 0 0 0 0 0 0 0 3 4
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 2 2 1 0 1 0 1 1 0 1 0 0 1 0 1 7 0 4 10 8
Vac/Vac Membrane 7 29 27 19 51 42 30 37 47 37 38 71 10 12 20 21 14 26 2 48 33 36 95 79
Unique Cell Count 38 298 184 200 271 381 395 535 301 366 407 411 116 194 176 91 131 114 71 339 201 145 324 266
Labelled Cell Count 50 337 206 220 310 520 549 743 442 499 588 571 123 203 190 107 150 131 71 339 201 145 324 266

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 4.6 4.6 4.7 5.3 3.7 3.7 3.9 3.5 3.7 3.5 3.5 5.3 5.4 5.6 6.4 6.4 6.5 4.8 5.1 5.2
Std Deviation (1e-4) 0.5 0.9 0.9 1.8 4.2 1.8 2.1 1.1 0.6 1.1 1.0 1.1 1.1 1.0 1.1 1.1 1.2 1.3 0.8 1.1 1.0
Intensity Change (Log2) 0.05 0.22 -0.3 -0.29 -0.24 -0.4 -0.31 -0.39 -0.38 0.22 0.23 0.29 0.49 0.49 0.5 0.07 0.15 0.2

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 0 0 0