Standard name | |
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Description | Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 4 | 2 | 4 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 7 | 8 | 11 | 4 | 12 | 12 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 1 | 6 | 11 | 7 | 9 | 15 | 8 | 8 | 10 | 11 | 4 | 22 | 44 | 33 | 21 | 14 | 0 | 0 | 0 | 0 | 3 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 5 | 0 | 0 | 1 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 20 | 12 | 74 | 104 | 89 | 142 | 212 | 206 | 167 | 281 | 165 | 237 | 7 | 3 | 33 | 14 | 9 | 26 | 1 | 0 | 0 | 0 | 0 | 3 |
Nucleus | 96 | 44 | 101 | 122 | 263 | 180 | 262 | 289 | 179 | 287 | 193 | 219 | 60 | 158 | 182 | 268 | 164 | 216 | 90 | 66 | 103 | 64 | 147 | 109 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 3 | 0 | 3 | 4 | 2 | 6 | 5 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 2 | 2 | 2 | 0 | 4 | 10 | 11 | 9 | 24 | 27 | 27 | 10 | 4 | 5 | 3 | 2 | 0 | 3 | 1 | 2 | 2 | 5 | 12 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 3 | 2 | 3 | 4 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 1 | 3 | 3 | 0 | 7 | 2 | 2 | 2 | 1 | 4 | 1 | 4 | 0 | 6 | 8 | 31 | 14 | 17 | 4 | 2 | 14 | 5 | 18 | 24 |
Unique Cell Count | 107 | 51 | 123 | 154 | 313 | 222 | 327 | 361 | 237 | 385 | 257 | 305 | 68 | 188 | 244 | 335 | 203 | 252 | 109 | 73 | 125 | 79 | 192 | 165 |
Labelled Cell Count | 122 | 62 | 182 | 236 | 377 | 347 | 509 | 543 | 373 | 626 | 420 | 515 | 81 | 194 | 278 | 358 | 218 | 288 | 109 | 73 | 125 | 79 | 192 | 165 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.5 | 3.6 | 3.6 | 4.3 | 3.7 | 3.5 | 3.7 | 3.4 | 3.4 | 3.5 | 3.2 | 5.6 | 5.9 | 5.0 | 6.6 | 6.8 | 7.2 | 5.5 | 5.5 | 6.4 |
Std Deviation (1e-4) | 0.7 | 0.6 | 0.7 | 0.9 | 1.4 | 1.0 | 1.0 | 1.2 | 1.4 | 1.1 | 1.2 | 1.0 | 1.0 | 1.1 | 1.3 | 1.6 | 1.6 | 1.5 | 1.2 | 1.4 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.25 | 0.04 | -0.05 | 0.04 | -0.07 | -0.1 | -0.05 | -0.17 | 0.62 | 0.71 | 0.47 | 0.86 | 0.92 | 0.99 | 0.59 | 0.6 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.5 | 1.8 | 1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0496 | 3.4088 | 3.2434 | 3.4814 | 2.29 | 2.9876 | 4.4201 | 5.1222 | 4.4303 | 4.3754 | 3.9823 | 4.3512 | 1.7302 | 1.8273 | 1.4681 | 1.6298 | 1.0383 | 1.4994 |
Actin | 0.0204 | 0.0112 | 0.011 | 0.0021 | 0.0285 | 0.0057 | 0.0192 | 0 | 0.0044 | 0.0001 | 0.0017 | 0.0024 | 0.0235 | 0 | 0.0008 | 0.0036 | 0 | 0.0002 |
Bud | 0.0005 | 0 | 0.0003 | 0.0008 | 0.0011 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0006 | 0 | 0.0011 | 0 | 0 |
Bud Neck | 0.0024 | 0.0002 | 0.0007 | 0.0007 | 0.0146 | 0.007 | 0.0016 | 0 | 0.0004 | 0.0002 | 0.0017 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0025 | 0.0004 | 0.0005 |
Bud Periphery | 0.0014 | 0 | 0.0005 | 0.0022 | 0.0021 | 0.0003 | 0.0006 | 0 | 0.0004 | 0.0005 | 0.001 | 0.0001 | 0.0005 | 0.0002 | 0 | 0.0038 | 0 | 0 |
Bud Site | 0.0102 | 0.0002 | 0.002 | 0.0035 | 0.0111 | 0.0002 | 0.0088 | 0 | 0.0044 | 0.0003 | 0.001 | 0 | 0.0009 | 0.0006 | 0.0001 | 0.0023 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0 | 0.0002 | 0.0006 | 0.0006 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0007 | 0 | 0 |
Cytoplasm | 0.0016 | 0.0001 | 0.0015 | 0.0007 | 0.0006 | 0.0001 | 0.0048 | 0 | 0.0058 | 0.0007 | 0.004 | 0.0003 | 0.0035 | 0.0491 | 0.0006 | 0.0011 | 0.0001 | 0.0002 |
Cytoplasmic Foci | 0.009 | 0.0001 | 0.0022 | 0.0005 | 0.0079 | 0.0004 | 0.0102 | 0 | 0.0025 | 0.0003 | 0.0049 | 0.0005 | 0.0041 | 0.0015 | 0.0034 | 0.0006 | 0 | 0.0012 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0031 | 0 | 0.0008 | 0.0013 | 0.0012 | 0.0007 | 0.0025 | 0 | 0.0003 | 0.0007 | 0.0017 | 0.001 | 0.0011 | 0.0004 | 0.0001 | 0.0008 | 0 | 0 |
Endosome | 0.0155 | 0 | 0.0068 | 0.008 | 0.0338 | 0.0018 | 0.0159 | 0 | 0.0032 | 0.0151 | 0.0229 | 0.0043 | 0.006 | 0.0014 | 0.001 | 0.0043 | 0 | 0.0007 |
Golgi | 0.0053 | 0.0001 | 0.0023 | 0.0034 | 0.0124 | 0.0018 | 0.0055 | 0 | 0.001 | 0.0006 | 0.0064 | 0.0022 | 0.0022 | 0.0001 | 0.0011 | 0.0014 | 0 | 0.0008 |
Lipid Particles | 0.0141 | 0 | 0.0038 | 0.0034 | 0.0421 | 0.0017 | 0.0139 | 0 | 0.0037 | 0.0003 | 0.0262 | 0.0003 | 0.003 | 0 | 0.0032 | 0.0017 | 0 | 0.0072 |
Mitochondria | 0.0083 | 0.0001 | 0.0135 | 0.0295 | 0.0214 | 0.0031 | 0.0068 | 0.0002 | 0.0067 | 0.0028 | 0.0248 | 0.0009 | 0.025 | 0.0002 | 0.0021 | 0.0233 | 0.0002 | 0.0006 |
None | 0.0014 | 0 | 0.0016 | 0.0004 | 0.0008 | 0.0001 | 0.0026 | 0 | 0.0005 | 0.0001 | 0.0015 | 0.0001 | 0.0026 | 0.0015 | 0.0007 | 0.0004 | 0 | 0.0002 |
Nuclear Periphery | 0.0085 | 0.0008 | 0.0058 | 0.0077 | 0.0234 | 0.0039 | 0.0139 | 0.0002 | 0.0029 | 0.0027 | 0.0111 | 0.0012 | 0.0063 | 0.0009 | 0.0015 | 0.0232 | 0.0019 | 0.0006 |
Nucleolus | 0.0151 | 0.0098 | 0.0118 | 0.0121 | 0.0341 | 0.0219 | 0.0062 | 0.006 | 0.0048 | 0.0059 | 0.0174 | 0.0159 | 0.0065 | 0.0105 | 0.0057 | 0.0084 | 0.0174 | 0.0132 |
Nucleus | 0.8646 | 0.9769 | 0.9196 | 0.9027 | 0.7124 | 0.9496 | 0.858 | 0.9903 | 0.9544 | 0.9483 | 0.8335 | 0.9629 | 0.8904 | 0.9251 | 0.961 | 0.9117 | 0.9783 | 0.9708 |
Peroxisomes | 0.008 | 0.0001 | 0.0095 | 0.0005 | 0.0076 | 0.0003 | 0.0126 | 0 | 0.0022 | 0.0001 | 0.0022 | 0.0001 | 0.0021 | 0 | 0.0089 | 0.0009 | 0 | 0.0013 |
Punctate Nuclear | 0.0055 | 0.0001 | 0.0032 | 0.0011 | 0.0309 | 0.0005 | 0.0114 | 0.0031 | 0.001 | 0.0002 | 0.0283 | 0.0069 | 0.0193 | 0.002 | 0.0093 | 0.0024 | 0.0014 | 0.0024 |
Vacuole | 0.0031 | 0.0001 | 0.0017 | 0.0091 | 0.0067 | 0.0005 | 0.002 | 0 | 0.0008 | 0.0145 | 0.0054 | 0.0001 | 0.0008 | 0.0048 | 0.0001 | 0.004 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0013 | 0 | 0.0011 | 0.0096 | 0.006 | 0.0004 | 0.0025 | 0 | 0.0002 | 0.0064 | 0.0037 | 0.0002 | 0.0011 | 0.0008 | 0 | 0.0018 | 0 | 0 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.4906 | 8.5216 | 11.066 | 16.2542 | 17.2518 | 15.482 | 16.6939 | 18.0518 | 15.3675 | 18.4139 |
Translational Efficiency | 0.7046 | 1.041 | 0.9407 | 0.5889 | 0.4921 | 0.7052 | 0.6191 | 0.5466 | 0.7087 | 0.7322 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |