Standard name
Description Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0.18 0.1 0.1 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.24 0.6 0.68 0.28 0.64 0.65 0.57 0.7 0.73 0.64 0.78 0.1 0 0.14 0 0 0.1 0 0 0 0 0 0
Nucleus 0.9 0.86 0.82 0.79 0.84 0.81 0.8 0.8 0.76 0.75 0.75 0.72 0.88 0.84 0.75 0.8 0.81 0.86 0.83 0.92 0.82 0.82 0.77 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.06 0.11 0.09 0.15 0 0 0 0 0 0 0 0 0 0 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.07 0 0 0.11 0.07 0.1 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 4 2 4 2 4 5 0 0 0 1 0 0 0 0 0 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 7 8 11 4 12 12 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 1 1 6 11 7 9 15 8 8 10 11 4 22 44 33 21 14 0 0 0 0 3 1
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 4 2 5 0 0 0 0 0 1
Endosome 0 0 0 0 1 0 0 0 1 0 0 0 0 0 2 1 3 5 0 0 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0
Mitochondria 20 12 74 104 89 142 212 206 167 281 165 237 7 3 33 14 9 26 1 0 0 0 0 3
Nucleus 96 44 101 122 263 180 262 289 179 287 193 219 60 158 182 268 164 216 90 66 103 64 147 109
Nuclear Periphery 0 0 0 1 3 0 3 4 2 6 5 1 0 1 1 0 0 1 0 0 0 0 0 0
Nucleolus 3 2 2 2 0 4 10 11 9 24 27 27 10 4 5 3 2 0 3 1 2 2 5 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 1 1 0 0 2 1 0 0 1 3 2 3 4 0 0 0 2 2
Vac/Vac Membrane 1 3 3 0 7 2 2 2 1 4 1 4 0 6 8 31 14 17 4 2 14 5 18 24
Unique Cell Count 107 51 123 154 313 222 327 361 237 385 257 305 68 188 244 335 203 252 109 73 125 79 192 165
Labelled Cell Count 122 62 182 236 377 347 509 543 373 626 420 515 81 194 278 358 218 288 109 73 125 79 192 165


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.5 3.6 3.6 4.3 3.7 3.5 3.7 3.4 3.4 3.5 3.2 5.6 5.9 5.0 6.6 6.8 7.2 5.5 5.5 6.4
Std Deviation (1e-4) 0.7 0.6 0.7 0.9 1.4 1.0 1.0 1.2 1.4 1.1 1.2 1.0 1.0 1.1 1.3 1.6 1.6 1.5 1.2 1.4 1.9
Intensity Change (Log2) – – – 0.01 0.25 0.04 -0.05 0.04 -0.07 -0.1 -0.05 -0.17 0.62 0.71 0.47 0.86 0.92 0.99 0.59 0.6 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.5 1.8 1.7