Standard name | |
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Description | Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 4 | 2 | 4 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 7 | 8 | 11 | 4 | 12 | 12 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 1 | 1 | 6 | 11 | 7 | 9 | 15 | 8 | 8 | 10 | 11 | 4 | 22 | 44 | 33 | 21 | 14 | 0 | 0 | 0 | 0 | 3 | 1 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 5 | 0 | 0 | 1 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 20 | 12 | 74 | 104 | 89 | 142 | 212 | 206 | 167 | 281 | 165 | 237 | 7 | 3 | 33 | 14 | 9 | 26 | 1 | 0 | 0 | 0 | 0 | 3 |
Nucleus | 96 | 44 | 101 | 122 | 263 | 180 | 262 | 289 | 179 | 287 | 193 | 219 | 60 | 158 | 182 | 268 | 164 | 216 | 90 | 66 | 103 | 64 | 147 | 109 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 3 | 0 | 3 | 4 | 2 | 6 | 5 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3 | 2 | 2 | 2 | 0 | 4 | 10 | 11 | 9 | 24 | 27 | 27 | 10 | 4 | 5 | 3 | 2 | 0 | 3 | 1 | 2 | 2 | 5 | 12 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 3 | 2 | 3 | 4 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 1 | 3 | 3 | 0 | 7 | 2 | 2 | 2 | 1 | 4 | 1 | 4 | 0 | 6 | 8 | 31 | 14 | 17 | 4 | 2 | 14 | 5 | 18 | 24 |
Unique Cell Count | 107 | 51 | 123 | 154 | 313 | 222 | 327 | 361 | 237 | 385 | 257 | 305 | 68 | 188 | 244 | 335 | 203 | 252 | 109 | 73 | 125 | 79 | 192 | 165 |
Labelled Cell Count | 122 | 62 | 182 | 236 | 377 | 347 | 509 | 543 | 373 | 626 | 420 | 515 | 81 | 194 | 278 | 358 | 218 | 288 | 109 | 73 | 125 | 79 | 192 | 165 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.5 | 3.6 | 3.6 | 4.3 | 3.7 | 3.5 | 3.7 | 3.4 | 3.4 | 3.5 | 3.2 | 5.6 | 5.9 | 5.0 | 6.6 | 6.8 | 7.2 | 5.5 | 5.5 | 6.4 |
Std Deviation (1e-4) | 0.7 | 0.6 | 0.7 | 0.9 | 1.4 | 1.0 | 1.0 | 1.2 | 1.4 | 1.1 | 1.2 | 1.0 | 1.0 | 1.1 | 1.3 | 1.6 | 1.6 | 1.5 | 1.2 | 1.4 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.25 | 0.04 | -0.05 | 0.04 | -0.07 | -0.1 | -0.05 | -0.17 | 0.62 | 0.71 | 0.47 | 0.86 | 0.92 | 0.99 | 0.59 | 0.6 | 0.83 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.5 | 1.8 | 1.7 |