Standard name
Description Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0.18 0.1 0.1 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.24 0.6 0.68 0.28 0.64 0.65 0.57 0.7 0.73 0.64 0.78 0.1 0 0.14 0 0 0.1 0 0 0 0 0 0
Nucleus 0.9 0.86 0.82 0.79 0.84 0.81 0.8 0.8 0.76 0.75 0.75 0.72 0.88 0.84 0.75 0.8 0.81 0.86 0.83 0.92 0.82 0.82 0.77 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.06 0.11 0.09 0.15 0 0 0 0 0 0 0 0 0 0 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.07 0 0 0.11 0.07 0.1 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 4 2 4 2 4 5 0 0 0 1 0 0 0 0 0 0 1 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 1 7 8 11 4 12 12 14 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 1 1 6 11 7 9 15 8 8 10 11 4 22 44 33 21 14 0 0 0 0 3 1
Endoplasmic Reticulum 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 4 2 5 0 0 0 0 0 1
Endosome 0 0 0 0 1 0 0 0 1 0 0 0 0 0 2 1 3 5 0 0 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0
Mitochondria 20 12 74 104 89 142 212 206 167 281 165 237 7 3 33 14 9 26 1 0 0 0 0 3
Nucleus 96 44 101 122 263 180 262 289 179 287 193 219 60 158 182 268 164 216 90 66 103 64 147 109
Nuclear Periphery 0 0 0 1 3 0 3 4 2 6 5 1 0 1 1 0 0 1 0 0 0 0 0 0
Nucleolus 3 2 2 2 0 4 10 11 9 24 27 27 10 4 5 3 2 0 3 1 2 2 5 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 1 1 0 0 2 1 0 0 1 3 2 3 4 0 0 0 2 2
Vac/Vac Membrane 1 3 3 0 7 2 2 2 1 4 1 4 0 6 8 31 14 17 4 2 14 5 18 24
Unique Cell Count 107 51 123 154 313 222 327 361 237 385 257 305 68 188 244 335 203 252 109 73 125 79 192 165
Labelled Cell Count 122 62 182 236 377 347 509 543 373 626 420 515 81 194 278 358 218 288 109 73 125 79 192 165


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.5 3.6 3.6 4.3 3.7 3.5 3.7 3.4 3.4 3.5 3.2 5.6 5.9 5.0 6.6 6.8 7.2 5.5 5.5 6.4
Std Deviation (1e-4) 0.7 0.6 0.7 0.9 1.4 1.0 1.0 1.2 1.4 1.1 1.2 1.0 1.0 1.1 1.3 1.6 1.6 1.5 1.2 1.4 1.9
Intensity Change (Log2) – – – 0.01 0.25 0.04 -0.05 0.04 -0.07 -0.1 -0.05 -0.17 0.62 0.71 0.47 0.86 0.92 0.99 0.59 0.6 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.5 1.8 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0496 3.4088 3.2434 3.4814 2.29 2.9876 4.4201 5.1222 4.4303 4.3754 3.9823 4.3512 1.7302 1.8273 1.4681 1.6298 1.0383 1.4994
Actin 0.0204 0.0112 0.011 0.0021 0.0285 0.0057 0.0192 0 0.0044 0.0001 0.0017 0.0024 0.0235 0 0.0008 0.0036 0 0.0002
Bud 0.0005 0 0.0003 0.0008 0.0011 0.0001 0.0005 0 0.0003 0.0002 0.0004 0.0001 0.0002 0.0006 0 0.0011 0 0
Bud Neck 0.0024 0.0002 0.0007 0.0007 0.0146 0.007 0.0016 0 0.0004 0.0002 0.0017 0.0006 0.0005 0.0002 0.0002 0.0025 0.0004 0.0005
Bud Periphery 0.0014 0 0.0005 0.0022 0.0021 0.0003 0.0006 0 0.0004 0.0005 0.001 0.0001 0.0005 0.0002 0 0.0038 0 0
Bud Site 0.0102 0.0002 0.002 0.0035 0.0111 0.0002 0.0088 0 0.0044 0.0003 0.001 0 0.0009 0.0006 0.0001 0.0023 0.0001 0.0001
Cell Periphery 0.0005 0 0.0002 0.0006 0.0006 0.0001 0.0002 0 0.0001 0.0001 0.0002 0 0.0001 0.0001 0 0.0007 0 0
Cytoplasm 0.0016 0.0001 0.0015 0.0007 0.0006 0.0001 0.0048 0 0.0058 0.0007 0.004 0.0003 0.0035 0.0491 0.0006 0.0011 0.0001 0.0002
Cytoplasmic Foci 0.009 0.0001 0.0022 0.0005 0.0079 0.0004 0.0102 0 0.0025 0.0003 0.0049 0.0005 0.0041 0.0015 0.0034 0.0006 0 0.0012
Eisosomes 0.0002 0 0.0001 0.0001 0.0006 0 0.0002 0 0.0001 0 0.0001 0 0.0004 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0031 0 0.0008 0.0013 0.0012 0.0007 0.0025 0 0.0003 0.0007 0.0017 0.001 0.0011 0.0004 0.0001 0.0008 0 0
Endosome 0.0155 0 0.0068 0.008 0.0338 0.0018 0.0159 0 0.0032 0.0151 0.0229 0.0043 0.006 0.0014 0.001 0.0043 0 0.0007
Golgi 0.0053 0.0001 0.0023 0.0034 0.0124 0.0018 0.0055 0 0.001 0.0006 0.0064 0.0022 0.0022 0.0001 0.0011 0.0014 0 0.0008
Lipid Particles 0.0141 0 0.0038 0.0034 0.0421 0.0017 0.0139 0 0.0037 0.0003 0.0262 0.0003 0.003 0 0.0032 0.0017 0 0.0072
Mitochondria 0.0083 0.0001 0.0135 0.0295 0.0214 0.0031 0.0068 0.0002 0.0067 0.0028 0.0248 0.0009 0.025 0.0002 0.0021 0.0233 0.0002 0.0006
None 0.0014 0 0.0016 0.0004 0.0008 0.0001 0.0026 0 0.0005 0.0001 0.0015 0.0001 0.0026 0.0015 0.0007 0.0004 0 0.0002
Nuclear Periphery 0.0085 0.0008 0.0058 0.0077 0.0234 0.0039 0.0139 0.0002 0.0029 0.0027 0.0111 0.0012 0.0063 0.0009 0.0015 0.0232 0.0019 0.0006
Nucleolus 0.0151 0.0098 0.0118 0.0121 0.0341 0.0219 0.0062 0.006 0.0048 0.0059 0.0174 0.0159 0.0065 0.0105 0.0057 0.0084 0.0174 0.0132
Nucleus 0.8646 0.9769 0.9196 0.9027 0.7124 0.9496 0.858 0.9903 0.9544 0.9483 0.8335 0.9629 0.8904 0.9251 0.961 0.9117 0.9783 0.9708
Peroxisomes 0.008 0.0001 0.0095 0.0005 0.0076 0.0003 0.0126 0 0.0022 0.0001 0.0022 0.0001 0.0021 0 0.0089 0.0009 0 0.0013
Punctate Nuclear 0.0055 0.0001 0.0032 0.0011 0.0309 0.0005 0.0114 0.0031 0.001 0.0002 0.0283 0.0069 0.0193 0.002 0.0093 0.0024 0.0014 0.0024
Vacuole 0.0031 0.0001 0.0017 0.0091 0.0067 0.0005 0.002 0 0.0008 0.0145 0.0054 0.0001 0.0008 0.0048 0.0001 0.004 0.0001 0.0001
Vacuole Periphery 0.0013 0 0.0011 0.0096 0.006 0.0004 0.0025 0 0.0002 0.0064 0.0037 0.0002 0.0011 0.0008 0 0.0018 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.4906 8.5216 11.066 16.2542 17.2518 15.482 16.6939 18.0518 15.3675 18.4139
Translational Efficiency 0.7046 1.041 0.9407 0.5889 0.4921 0.7052 0.6191 0.5466 0.7087 0.7322

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘