Standard name
Human Ortholog
Description RNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0 0 0 0 0 0 0 0 0.08 0.12 0.13 0.09 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.08 0 0.1 0.08 0.26 0.3 0.25 0.34 0.36 0.44 0.4 0 0 0 0.08 0.05 0.07 0 0 0 0 0 0
Nucleus 0.96 0.97 0.96 0.87 0.92 0.89 0.86 0.85 0.83 0.84 0.83 0.78 0.91 0.86 0.85 0.85 0.87 0.85 0.93 0.92 0.91 0.95 0.85 0.77
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0.07 0.1 0.13 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0.05 0 0.1 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 1 1 0 1 3 4 4 2 6 3 4 0 0 0 0 0 0 1 1 0 0 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 8 14 21 4 12 16 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 1 6 17 13 13 18 20 7 9 7 9 30 60 68 10 6 7 1 0 0 1 4 7
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 1 2 4 0 0 0 0 1 0
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 2 5 0 0 0 0 1 0 0 1 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0
Mitochondria 92 9 8 20 27 106 151 125 141 160 205 159 3 1 1 9 5 6 4 1 1 0 3 8
Nucleus 320 112 278 178 311 369 438 434 345 373 390 310 352 420 432 91 84 75 308 109 262 183 393 367
Nuclear Periphery 1 0 0 4 2 2 8 10 3 4 14 11 2 10 7 1 1 0 0 0 0 0 1 1
Nucleolus 1 0 0 1 3 9 21 25 31 46 59 33 1 0 2 0 0 1 0 0 1 0 3 9
Peroxisomes 0 0 0 0 1 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 2 0 1 0 0 1 1 5 0 0 0 1 0 0 0 2 5
Vac/Vac Membrane 2 2 2 1 6 7 6 9 5 5 6 9 1 3 1 5 6 5 9 4 14 5 46 61
Unique Cell Count 335 116 289 204 339 413 507 509 418 443 471 395 386 488 511 107 97 88 331 119 287 194 463 475
Labelled Cell Count 422 125 296 223 366 517 660 650 538 617 700 544 392 499 523 117 104 98 331 119 287 194 463 475


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.6 5.8 5.5 5.1 4.6 4.6 4.6 4.6 4.5 4.3 4.3 7.2 7.0 6.8 7.1 7.6 7.5 6.6 7.0 7.4
Std Deviation (1e-4) 0.6 1.0 1.3 2.0 1.4 1.4 1.3 1.3 1.1 1.1 1.0 1.2 1.7 1.6 1.5 1.5 1.4 1.6 0.8 1.3 1.7
Intensity Change (Log2) – – – -0.08 -0.18 -0.34 -0.35 -0.33 -0.34 -0.38 -0.44 -0.42 0.31 0.27 0.22 0.29 0.39 0.37 0.18 0.27 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 1.3 0.9 1.2 1.4 -0.4 0 0 0.2 3.3 4.9 5.3 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 2.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -3.7 -2.3 -3.3 -4.4 -4.8 -5.5 -5.1 -5.5 -6.6 -2.6 -4.5 -5.0 -3.9 -3.4 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6273 1.6545 1.3187 1.1783 0.7906 1.439 1.5424 1.7397 1.7776 1.1248 1.5931 1.5077 0.6947 1.3368 0.5825 0.8835 0.6428 0.8328
Actin 0.0086 0.0007 0.0143 0.0135 0.0004 0.0007 0.0211 0 0.0121 0 0.0092 0.0028 0 0 0.0022 0 0.0027 0.0083
Bud 0.0004 0.0002 0.0001 0 0.0002 0.0001 0.0004 0 0.0006 0 0.0012 0.0006 0 0 0.0001 0 0.0011 0.0001
Bud Neck 0.0016 0.0001 0.0003 0.0005 0.0004 0.0005 0.001 0.0001 0.0012 0 0.0003 0.0014 0 0 0.0001 0 0.0005 0.0007
Bud Periphery 0.0011 0 0.0004 0.0001 0.0002 0.0004 0.0007 0 0.0016 0 0.0014 0.0007 0 0 0.0001 0 0.0019 0.0002
Bud Site 0.0012 0.0001 0.0006 0.0008 0.0007 0.0002 0.0011 0 0.0022 0 0.0016 0.0021 0 0 0.0002 0 0.0062 0.0001
Cell Periphery 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0003 0 0.0002 0.0001 0 0 0 0 0.0001 0
Cytoplasm 0.0078 0.0004 0.0011 0.0005 0.0026 0.0001 0.0127 0.0002 0.0026 0 0.0046 0.0009 0.0008 0.0001 0.0003 0 0.0016 0.0004
Cytoplasmic Foci 0.0075 0.0026 0.0081 0.0049 0.0014 0.0002 0.0109 0 0.004 0 0.0022 0.0058 0.0028 0 0.0064 0 0.0095 0.0003
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0.0002 0 0 0 0 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0027 0 0.001 0.0001 0.0006 0.0001 0.001 0 0.0004 0 0.008 0.0004 0.0004 0 0.0002 0 0.0004 0.001
Endosome 0.0142 0.0005 0.0032 0.0013 0.0065 0.0014 0.0124 0 0.0053 0 0.0502 0.0092 0.0029 0 0.0027 0 0.0068 0.0015
Golgi 0.0027 0.0004 0.0015 0.0011 0.0004 0.0006 0.0042 0 0.0017 0 0.0086 0.002 0.0001 0 0.0009 0 0.0021 0.0005
Lipid Particles 0.0067 0.0022 0.0062 0.0019 0.0033 0.0007 0.0186 0 0.0036 0 0.001 0.0018 0.0025 0 0.0062 0 0.0053 0.001
Mitochondria 0.003 0.0002 0.0076 0.0002 0.0006 0.0018 0.0015 0.0001 0.0045 0 0.0065 0.0034 0.0002 0.0001 0.0011 0.0002 0.0051 0.0005
None 0.0048 0.0002 0.0017 0.0021 0.0022 0.0002 0.0012 0 0.0004 0 0.0007 0.0003 0.0004 0 0.0002 0 0.0029 0.0004
Nuclear Periphery 0.035 0.0037 0.008 0.0012 0.0082 0.0013 0.0048 0.0018 0.002 0.0001 0.0122 0.0052 0.0012 0.0023 0.0018 0.0005 0.0124 0.0055
Nucleolus 0.0074 0.007 0.0054 0.0025 0.0131 0.0182 0.0055 0.0035 0.003 0.0016 0.0128 0.0105 0.0063 0.004 0.0042 0.0031 0.0082 0.0092
Nucleus 0.8695 0.9693 0.9341 0.9608 0.9455 0.9676 0.8911 0.994 0.9474 0.9983 0.8706 0.9439 0.9774 0.9933 0.9637 0.9961 0.8809 0.9679
Peroxisomes 0.002 0.0029 0.0013 0.0027 0.0005 0.0002 0.0045 0 0.0031 0 0.0002 0.0026 0.0001 0 0.0051 0 0.0122 0.0005
Punctate Nuclear 0.0185 0.0091 0.0037 0.0054 0.0108 0.0051 0.0056 0.0001 0.001 0 0.0007 0.004 0.0045 0.0001 0.0015 0.0001 0.0387 0.0012
Vacuole 0.0031 0.0001 0.0007 0 0.0016 0.0004 0.001 0.0001 0.0022 0 0.0039 0.0018 0.0001 0 0.0021 0 0.001 0.0003
Vacuole Periphery 0.0019 0.0001 0.0005 0 0.0006 0.0003 0.0005 0.0001 0.0007 0 0.004 0.0007 0.0001 0 0.0007 0 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.2024 6.8677 9.5553 10.0943 11.2058 7.6783 9.1139 9.3734 9.6328 9.4452
Translational Efficiency 2.969 2.0736 1.9119 1.7833 1.7531 2.4411 1.503 1.66 1.6409 1.8615

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1680 1356 1641 1201 1188 1889 1104 1210 2868 3245 2745 2411

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 776.38 857.20 946.61 962.55 740.40 846.46 956.01 1009.60 761.48 850.95 950.39 986.16
Standard Deviation 104.10 117.96 128.27 140.71 100.31 111.44 130.35 142.23 104.07 114.33 129.19 143.42
Intensity Change Log 2 — 0.142869 0.286007 0.310099 — 0.193137 0.368721 0.447407 — 0.167626 0.326976 0.378757

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000152 0.000360 0.000484 0.001440 0.000124 0.000246 0.000686 0.001134 0.000141 0.000294 0.000565 0.001287
Bud Neck 0.003152 0.005571 0.015728 0.016296 0.001416 0.003388 0.007749 0.017768 0.002433 0.004300 0.012519 0.017035
Bud Site 0.000535 0.001654 0.001275 0.002761 0.000446 0.001059 0.000815 0.007800 0.000498 0.001308 0.001090 0.005290
Cell Periphery 0.000092 0.000166 0.000083 0.000074 0.000247 0.000351 0.000063 0.000209 0.000156 0.000274 0.000075 0.000142
Cytoplasm 0.007049 0.002581 0.003035 0.002292 0.010934 0.003722 0.003103 0.009564 0.008658 0.003245 0.003062 0.005941
Cytoplasmic Foci 0.000726 0.000866 0.000198 0.000686 0.000879 0.001194 0.000058 0.003175 0.000789 0.001056 0.000142 0.001935
Eisosomes 0.000010 0.000020 0.000025 0.000027 0.000014 0.000017 0.000024 0.000028 0.000012 0.000018 0.000024 0.000028
Endoplasmic Reticulum 0.003425 0.007791 0.005340 0.007568 0.003363 0.004020 0.005643 0.009278 0.003399 0.005596 0.005462 0.008426
Endosome 0.000617 0.000658 0.000267 0.000513 0.000578 0.000978 0.000165 0.005028 0.000601 0.000844 0.000226 0.002779
Golgi 0.000326 0.000184 0.000043 0.000573 0.000037 0.001121 0.000042 0.004153 0.000206 0.000730 0.000042 0.002370
Lipid Particles 0.000391 0.000324 0.000100 0.000234 0.000448 0.000428 0.000114 0.000498 0.000415 0.000385 0.000106 0.000367
Mitochondria 0.001704 0.002064 0.001096 0.001739 0.001111 0.002632 0.000685 0.006587 0.001458 0.002395 0.000931 0.004172
Mitotic Spindle 0.002131 0.004104 0.000890 0.000623 0.000186 0.003769 0.000310 0.022228 0.001325 0.003909 0.000657 0.011466
None 0.003179 0.002984 0.003055 0.001991 0.008710 0.003326 0.003685 0.005244 0.005470 0.003183 0.003308 0.003624
Nuclear Periphery 0.000842 0.000827 0.000557 0.000952 0.000784 0.000914 0.000383 0.002465 0.000818 0.000878 0.000487 0.001711
Nuclear Periphery Foci 0.000378 0.000306 0.000108 0.000121 0.000225 0.000409 0.000075 0.000836 0.000314 0.000366 0.000095 0.000480
Nucleolus 0.004073 0.004821 0.003503 0.003119 0.003995 0.004159 0.003495 0.002347 0.004040 0.004435 0.003500 0.002731
Nucleus 0.968965* 0.961482* 0.962046* 0.956899* 0.964404* 0.965026* 0.971476* 0.886666* 0.967075* 0.963545* 0.965839* 0.921651*
Peroxisomes 0.000873 0.000608 0.000109 0.000142 0.000167 0.000769 0.000124 0.000501 0.000580 0.000702 0.000115 0.000322
Vacuole 0.000851 0.002336 0.001903 0.001811 0.001628 0.001775 0.001241 0.010442 0.001173 0.002009 0.001637 0.006143
Vacuole Periphery 0.000530 0.000294 0.000157 0.000139 0.000304 0.000698 0.000062 0.004049 0.000437 0.000529 0.000119 0.002101

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.20 -11.36 -3.09 -2.54 -2.06 -2.49 -3.34 -9.28 -7.87 -1.41 -4.19 -5.75 -5.77 -4.99 -2.77
Bud Neck -3.24 -11.51 -10.30 -8.40 -1.17 -4.31 -11.69 -14.39 -12.58 -7.74 -4.16 -14.87 -16.91 -14.84 -4.91
Bud Site -2.62 -4.60 -3.25 -1.33 -1.72 -1.79 -3.09 -5.10 -4.38 -4.39 -3.05 -5.55 -6.03 -4.42 -4.53
Cell Periphery -2.43 0.55 1.11 3.04 0.80 -0.38 3.22 1.52 0.76 -2.69 -0.76 3.22 1.45 1.04 -2.27
Cytoplasm 4.23 3.72 4.54 0.36 1.12 5.13 4.92 0.55 -4.81 -4.57 6.34 6.13 2.47 -4.19 -4.03
Cytoplasmic Foci -0.62 2.37 0.60 0.98 -0.64 -0.71 4.49 -1.18 -0.67 -2.85 -1.05 4.30 -0.58 0.16 -2.59
Eisosomes -5.79 -13.03 -5.83 -2.70 -1.03 -0.85 -8.87 -11.79 -6.77 -4.02 -3.53 -15.54 -11.21 -6.35 -3.15
Endoplasmic Reticulum -7.78 -6.52 -9.25 -0.03 -4.56 -1.29 -6.48 -11.31 -11.32 -7.67 -6.31 -9.14 -14.86 -8.49 -8.89
Endosome -0.24 1.43 0.57 1.04 -1.14 -1.13 1.54 -2.92 -2.37 -3.55 -1.09 2.06 -2.57 -1.95 -3.67
Golgi 0.74 1.64 -0.28 -0.76 -1.25 -2.18 -2.34 -2.63 -1.02 -2.60 -1.71 1.54 -2.44 -1.01 -2.88
Lipid Particles 0.36 1.97 1.01 0.90 -0.99 0.25 3.22 0.14 -0.09 -2.70 0.29 3.13 0.81 0.66 -2.68
Mitochondria -0.43 0.72 -0.64 -0.04 -4.34 -1.90 0.51 -3.80 -2.09 -4.86 -1.57 0.87 -3.40 -1.71 -5.59
Mitotic Spindle -1.26 0.81 -0.41 1.03 -1.39 -3.19 -2.16 -6.12 -4.77 -5.94 -2.63 0.35 -5.78 -3.95 -6.01
None 0.22 0.33 2.21 1.28 1.45 3.34 3.08 2.88 -0.57 -0.31 2.73 2.80 3.16 0.30 0.31
Nuclear Periphery -0.01 1.72 -3.10 -2.76 -5.38 -0.65 1.86 -6.72 -6.06 -8.06 -0.45 2.48 -7.30 -6.53 -9.50
Nuclear Periphery Foci 0.17 1.65 0.14 0.03 -0.89 -1.19 0.81 -2.96 -1.70 -3.33 -0.54 1.83 -1.51 -1.09 -2.81
Nucleolus -1.08 0.25 0.19 1.59 -0.06 -0.30 0.16 1.79 1.68 1.43 -0.80 0.30 1.12 2.10 1.06
Nucleus 1.91 3.19 5.26 2.94 2.73 -0.16 -0.47 11.18 11.34 12.12 1.24 2.30 12.29 11.09 11.44
Peroxisomes 0.65 2.01 1.94 2.66 -0.84 -2.33 0.35 -2.36 1.62 -2.57 -0.38 2.03 1.54 2.73 -2.81
Vacuole -1.85 -3.34 -5.33 -1.22 -1.99 -0.26 -0.98 -7.77 -7.65 -7.62 -1.71 -3.05 -8.87 -7.51 -7.77
Vacuole Periphery 0.80 1.28 1.35 1.83 0.53 -1.56 1.60 -2.42 -1.83 -2.80 -0.44 1.74 -1.68 -1.52 -2.73
Standard name
Human Ortholog
Description RNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Slu7

Slu7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slu7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available