Standard name | |
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Description | RNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 1 | 0 | 1 | 3 | 4 | 4 | 2 | 6 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 8 | 14 | 21 | 4 | 12 | 16 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 1 | 6 | 17 | 13 | 13 | 18 | 20 | 7 | 9 | 7 | 9 | 30 | 60 | 68 | 10 | 6 | 7 | 1 | 0 | 0 | 1 | 4 | 7 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 92 | 9 | 8 | 20 | 27 | 106 | 151 | 125 | 141 | 160 | 205 | 159 | 3 | 1 | 1 | 9 | 5 | 6 | 4 | 1 | 1 | 0 | 3 | 8 |
Nucleus | 320 | 112 | 278 | 178 | 311 | 369 | 438 | 434 | 345 | 373 | 390 | 310 | 352 | 420 | 432 | 91 | 84 | 75 | 308 | 109 | 262 | 183 | 393 | 367 |
Nuclear Periphery | 1 | 0 | 0 | 4 | 2 | 2 | 8 | 10 | 3 | 4 | 14 | 11 | 2 | 10 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 1 | 0 | 0 | 1 | 3 | 9 | 21 | 25 | 31 | 46 | 59 | 33 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 9 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 5 |
Vac/Vac Membrane | 2 | 2 | 2 | 1 | 6 | 7 | 6 | 9 | 5 | 5 | 6 | 9 | 1 | 3 | 1 | 5 | 6 | 5 | 9 | 4 | 14 | 5 | 46 | 61 |
Unique Cell Count | 335 | 116 | 289 | 204 | 339 | 413 | 507 | 509 | 418 | 443 | 471 | 395 | 386 | 488 | 511 | 107 | 97 | 88 | 331 | 119 | 287 | 194 | 463 | 475 |
Labelled Cell Count | 422 | 125 | 296 | 223 | 366 | 517 | 660 | 650 | 538 | 617 | 700 | 544 | 392 | 499 | 523 | 117 | 104 | 98 | 331 | 119 | 287 | 194 | 463 | 475 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 4.5 | 5.6 | 5.8 | 5.5 | 5.1 | 4.6 | 4.6 | 4.6 | 4.6 | 4.5 | 4.3 | 4.3 | 7.2 | 7.0 | 6.8 | 7.1 | 7.6 | 7.5 | 6.6 | 7.0 | 7.4 |
Std Deviation (1e-4) | 0.6 | 1.0 | 1.3 | 2.0 | 1.4 | 1.4 | 1.3 | 1.3 | 1.1 | 1.1 | 1.0 | 1.2 | 1.7 | 1.6 | 1.5 | 1.5 | 1.4 | 1.6 | 0.8 | 1.3 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.18 | -0.34 | -0.35 | -0.33 | -0.34 | -0.38 | -0.44 | -0.42 | 0.31 | 0.27 | 0.22 | 0.29 | 0.39 | 0.37 | 0.18 | 0.27 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.2 | 1.3 | 0.9 | 1.2 | 1.4 | -0.4 | 0 | 0 | 0.2 | 3.3 | 4.9 | 5.3 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.3 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -3.7 | -2.3 | -3.3 | -4.4 | -4.8 | -5.5 | -5.1 | -5.5 | -6.6 | -2.6 | -4.5 | -5.0 | -3.9 | -3.4 | -3.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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