Standard name
Description RNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0 0 0 0 0 0 0 0 0.08 0.12 0.13 0.09 0.06 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.08 0 0.1 0.08 0.26 0.3 0.25 0.34 0.36 0.44 0.4 0 0 0 0.08 0.05 0.07 0 0 0 0 0 0
Nucleus 0.96 0.97 0.96 0.87 0.92 0.89 0.86 0.85 0.83 0.84 0.83 0.78 0.91 0.86 0.85 0.85 0.87 0.85 0.93 0.92 0.91 0.95 0.85 0.77
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0.07 0.1 0.13 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0.05 0 0.1 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 1 1 0 1 3 4 4 2 6 3 4 0 0 0 0 0 0 1 1 0 0 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 8 14 21 4 12 16 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 1 6 17 13 13 18 20 7 9 7 9 30 60 68 10 6 7 1 0 0 1 4 7
Endoplasmic Reticulum 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 1 2 4 0 0 0 0 1 0
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 2 5 0 0 0 0 1 0 0 1 2
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0
Mitochondria 92 9 8 20 27 106 151 125 141 160 205 159 3 1 1 9 5 6 4 1 1 0 3 8
Nucleus 320 112 278 178 311 369 438 434 345 373 390 310 352 420 432 91 84 75 308 109 262 183 393 367
Nuclear Periphery 1 0 0 4 2 2 8 10 3 4 14 11 2 10 7 1 1 0 0 0 0 0 1 1
Nucleolus 1 0 0 1 3 9 21 25 31 46 59 33 1 0 2 0 0 1 0 0 1 0 3 9
Peroxisomes 0 0 0 0 1 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 2 0 1 0 0 1 1 5 0 0 0 1 0 0 0 2 5
Vac/Vac Membrane 2 2 2 1 6 7 6 9 5 5 6 9 1 3 1 5 6 5 9 4 14 5 46 61
Unique Cell Count 335 116 289 204 339 413 507 509 418 443 471 395 386 488 511 107 97 88 331 119 287 194 463 475
Labelled Cell Count 422 125 296 223 366 517 660 650 538 617 700 544 392 499 523 117 104 98 331 119 287 194 463 475


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.6 5.8 5.5 5.1 4.6 4.6 4.6 4.6 4.5 4.3 4.3 7.2 7.0 6.8 7.1 7.6 7.5 6.6 7.0 7.4
Std Deviation (1e-4) 0.6 1.0 1.3 2.0 1.4 1.4 1.3 1.3 1.1 1.1 1.0 1.2 1.7 1.6 1.5 1.5 1.4 1.6 0.8 1.3 1.7
Intensity Change (Log2) – – – -0.08 -0.18 -0.34 -0.35 -0.33 -0.34 -0.38 -0.44 -0.42 0.31 0.27 0.22 0.29 0.39 0.37 0.18 0.27 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.2 1.3 0.9 1.2 1.4 -0.4 0 0 0.2 3.3 4.9 5.3 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 2.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -3.7 -2.3 -3.3 -4.4 -4.8 -5.5 -5.1 -5.5 -6.6 -2.6 -4.5 -5.0 -3.9 -3.4 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slu7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available